{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:47:56Z","timestamp":1785415676697,"version":"3.56.0"},"reference-count":85,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Special Fund Project China Agriculture Research System of MOF and MARA","award":["CARS-47"],"award-info":[{"award-number":["CARS-47"]}]},{"award":["CARS-47"],"award-info":[{"award-number":["CARS-47"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Guangdong Provincial Special Fund Project for Talent Development Strategy","award":["2024R1004"],"award-info":[{"award-number":["2024R1004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>This study investigated the effects of high-lipid diets (HLD) with methionine (Met) supplementations on golden pompano (Trachinotus ovatus). T. ovatus (initial weight 82 \u00b1 0.04 g) were fed with a normal lipid control diet (11.30% crude lipid and 1.04% Met, NLM) and HLDs (18% crude lipid) supplemented with varying Met levels (1.04%, 1.14%, 1.24%, 1.34%, 1.44%, 1.54% and 1.64%) namely HLM1, HLM2, HLM3, HLM4, HLM5, HLM6 and HLM7, respectively. After 56 days of feeding trial, the growth performance such as weight gain rate was significantly elevated in the HLM3 (p &lt; 0.05). The liver lipid droplets area, sum of n-3 and n-6 poly-unsaturated fatty acids and fatty acid synthesis genes were elevated in the HLM1 and HLM7, while the genes for lipid breakdown were elevated in the HLM3. Based on the groups NLM, HLM1, and HLM3, the transcriptome sequence data revealed critical associated lipid metabolism, classic antioxidant pathway nrf2 and ferroptosis markers were influenced by Met and higher lipid. The fish livers in HLD groups with lower or higher Met showed the phenomenon of lipid oxidation obviously, while the reactive oxygen species and malondialdehyde were considerably lowered in the liver of fish in group HLM3 (p &lt; 0.05). The hepatic Met was significantly reduced while cysteine was elevated in the HLM3 compared to HLM7 (p &lt; 0.05). Fe and ferroptosis inducers were significantly upregulated in the liver of HLM1 and HLM7. HLM3 elevated anti-ferroptosis and anti-inflammation markers. In conclusion, Met inclusion in the HLD was associated with nrf2\/keap1 and critical anti-ferroptosis-related transcriptional responses and regulated lipid metabolism in T. ovatus. The quadratic regression model revealed the optimal dietary Met in the HLD as 1.32%, which will help formulators make more rational and effective use of dietary lipid to support the better growth of golden pompano.<\/jats:p>","DOI":"10.3390\/antiox15070873","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T05:32:01Z","timestamp":1784007121000},"page":"873","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary Methionine Affects Lipid Metabolism and Ferroptosis-Related Responses to Modulate Oxidative Stress Induced by High-Lipid-Diet in Golden Pompano (Trachinotus ovatus)"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9587-7894","authenticated-orcid":false,"given":"Bissih","family":"Fred","sequence":"first","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaimin","family":"Cheng","sequence":"additional","affiliation":[{"name":"Guangdong Yuehai Feeds Group, Zhanjiang 524000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agyenim Godfred","family":"Boateng","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2102-2092","authenticated-orcid":false,"given":"Junming","family":"Deng","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beiping","family":"Tan","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asare","family":"Derrick","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyan","family":"Chi","sequence":"additional","affiliation":[{"name":"College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China"},{"name":"Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1007\/s00360-025-01626-z","article-title":"Nutritional and physiological effects of high-fat diets in finfish: Effects on growth, immunity, lipid metabolism, and intestinal health: A review","volume":"195","author":"Desouky","year":"2025","journal-title":"J. Comp. Physiol. B"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hardy, R.W., Kaushik, S.J., Mai, K., and Bai, S.C. (2022). Fish nutrition\u2014History and perspectives. Fish Nutrition, Academic Press.","DOI":"10.1016\/B978-0-12-819587-1.00006-9"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"100496","DOI":"10.1016\/j.aqrep.2020.100496","article-title":"Precision nutritional regulation and aquaculture","volume":"18","author":"Zhang","year":"2020","journal-title":"Aquac. Rep."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"213","DOI":"10.2331\/suisan.42.213","article-title":"Protein nutrition in fish. VI. Effects of dietary energy sources on the utilization of proteins by rainbow trout and carp","volume":"42","author":"Ogino","year":"1976","journal-title":"Nippon Suisan Gakkaishi"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1007\/s10695-024-01392-9","article-title":"Protein-sparing effects of lipids in the diet of golden pompano (Trachinotus ovatus): Evaluation of growth, feed utilization, and lipid metabolism","volume":"50","author":"Li","year":"2024","journal-title":"Fish Physiol. Biochem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1080\/23308249.2024.2388563","article-title":"Omega-3 futures in aquaculture: Exploring the supply and demands for long-chain omega-3 essential fatty acids by aquaculture species","volume":"33","author":"Glencross","year":"2025","journal-title":"Rev. Fish. Sci. Aquac."},{"key":"ref_7","first-page":"e431","article-title":"Effects of high carbohydrate and high lipid diets on growth, body composition and glucose metabolism in southern catfish at two temperatures","volume":"41","author":"Luo","year":"2010","journal-title":"Aquac. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s10695-022-01077-1","article-title":"Dietary calcium pyruvate could improve growth performance and reduce excessive lipid deposition in juvenile golden pompano (Trachinotus ovatus) fed a high-fat diet","volume":"48","author":"Shao","year":"2022","journal-title":"Fish Physiol. Biochem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5889","DOI":"10.7150\/thno.56157","article-title":"High fat diet, gut microbiome and gastrointestinal cancer","volume":"11","author":"Tong","year":"2021","journal-title":"Theranostics"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1111\/jpn.13759","article-title":"The risk assessment of high-fat diet in farmed fish and its mitigation approaches: A review","volume":"107","author":"Naiel","year":"2023","journal-title":"J. Anim. Physiol. Anim. Nutr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1005455","DOI":"10.1155\/anu\/1005455","article-title":"Tissue-specific mechanism of fat distribution in teleosts: Comparative analysis between two carnivorous marine species, golden pompano (Trachinotus ovatus) and spotted sea bass (Lateolabrax maculatus)","volume":"2025","author":"Su","year":"2025","journal-title":"Aquac. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Jin, M., Shen, Y., Pan, T., Zhu, T., Li, X., Xu, F., Betancor, M.B., Jiao, L., Tocher, D.R., and Zhou, Q. (2021). Dietary betaine mitigates hepatic steatosis and inflammation induced by a high-fat diet by modulating the Sirt1\/Srebp-1\/Ppar\u03b1 pathway in juvenile black seabream (Acanthopagrus schlegelii). Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.694720"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"740306","DOI":"10.1016\/j.aquaculture.2023.740306","article-title":"The ameliorative role of methionine in hepatic steatosis and stress response in juvenile black seabream (Acanthopagrus schlegelii) fed with a high-fat diet","volume":"580","author":"Yang","year":"2024","journal-title":"Aquaculture"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"736388","DOI":"10.1016\/j.aquaculture.2021.736388","article-title":"Effect of dietary methionine on growth performance, lipid metabolism and antioxidant capacity of large yellow croaker (Larimichthys crocea) fed with high lipid diets","volume":"536","author":"Li","year":"2021","journal-title":"Aquaculture"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"739310","DOI":"10.1016\/j.aquaculture.2023.739310","article-title":"Methionine in fish health and nutrition: Potential mechanisms, affecting factors, and future perspectives","volume":"568","author":"Wang","year":"2023","journal-title":"Aquaculture"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1017\/S0007114512001663","article-title":"Methionine restriction affects the phenotypic and transcriptional response of rainbow trout (Oncorhynchus mykiss) to carbohydrate-enriched diets","volume":"109","author":"Craig","year":"2013","journal-title":"Br. J. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"9970252","DOI":"10.1155\/2023\/9970252","article-title":"Effects of Dietary Protein and Lipid Levels in Practical Formulation on Growth, Feed Utilization, Body Composition, and Serum Biochemical Parameters of Growing Rockfish (Sebastes schlegeli)","volume":"2023","author":"Li","year":"2023","journal-title":"Aquac. Nutr."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Ma, C., Xu, A., Zuo, L., Li, Q., Fan, F., Hu, Y., and Sun, C. (2025). Methionine Dependency and Restriction in Cancer: Exploring the Pathogenic Function and Therapeutic Potential. Pharmaceuticals, 18.","DOI":"10.3390\/ph18050640"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"742562","DOI":"10.1016\/j.aquaculture.2025.742562","article-title":"Dietary bile acids reduced ferroptosis, improved the flesh quality and intestinal health of Pacific white shrimp (Litopenaeus vannamei) fed the high plant protein-based diets","volume":"605","author":"Qu","year":"2025","journal-title":"Aquaculture"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1111\/raq.12719","article-title":"The role of ferroptosis in fish inflammation","volume":"15","author":"Gao","year":"2023","journal-title":"Rev. Aquac."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"736760","DOI":"10.1016\/j.aquaculture.2021.736760","article-title":"Ferroptosis in aquaculture research","volume":"541","author":"Xia","year":"2021","journal-title":"Aquaculture"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"He, L., Zhang, Y., Cao, Q., Shan, H., Zong, J., Feng, L., Jiang, W., Wu, P., Zhao, J., and Liu, H. (2024). Hepatic Oxidative Stress and Cell Death Influenced by Dietary Lipid Levels in a Fresh Teleost. Antioxidants, 13.","DOI":"10.3390\/antiox13070808"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jare.2023.11.030","article-title":"The male and female genomes of golden pompano (Trachinotus ovatus) provide insights into the sex chromosome evolution and rapid growth","volume":"65","author":"Luo","year":"2023","journal-title":"J. Adv. Res."},{"key":"ref_24","unstructured":"FAO (2024). The State of World Fisheries and Aquaculture 2024\u2014Blue Transformation in Action, FAO."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"101","DOI":"10.26599\/FSHW.2022.9250008","article-title":"Insights into microbiota community dynamics and flavor development mechanism during golden pomfret (Trachinotus ovatus) fermentation based on single-molecule real-time sequencing and molecular networking analysis","volume":"13","author":"Wang","year":"2024","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_26","unstructured":"Bureau of Fisheries, Ministry of Agriculture and Rural Affairs, National Fisheries Technology Extension Center, and China Society of Fisheries (2025). China Fishery Statistical Yearbook 2025, China Agriculture Press."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Chen, X., Niu, J., Wang, J., and Zhao, W. (2022). Effects of L-carnitine supplementation in high-fat diet on growth, antioxidant capacity and lipid metabolism of golden pompano (Trachinotus ovatus). Front. Mar. Sci., 9.","DOI":"10.3389\/fmars.2022.831153"},{"key":"ref_28","unstructured":"(2019). Determination of Amino Acids in Feeds (Standard No. GB\/T 18246-2019)."},{"key":"ref_29","unstructured":"(2016). National Food Safety Standard\u2014Determination of Fatty Acids in Foods (Standard No. GB 5009.168-2016)."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"735014","DOI":"10.1016\/j.aquaculture.2020.735014","article-title":"The optimum methionine requirement in diets of juvenile hybrid grouper (Epinephelus fuscoguttatus \u2640 \u00d7 Epinephelus lanceolatus \u2642): Effects on survival, growth performance, gut micromorphology and immunity","volume":"520","author":"Li","year":"2020","journal-title":"Aquaculture"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/anu.13006","article-title":"Dietary methionine affects growth and the expression of key genes involved in hepatic lipogenesis and glucose metabolism in cobia (Rachycentron canadum)","volume":"26","author":"Chi","year":"2020","journal-title":"Aquac. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.aquaculture.2018.08.053","article-title":"Effect of dietary methionine levels on growth performance, amino acid metabolism and intestinal homeostasis in turbot (Scophthalmus maximus L.)","volume":"498","author":"Gao","year":"2019","journal-title":"Aquaculture"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.aquaculture.2017.06.017","article-title":"Effects of dietary methionine and taurine supplementation to low-fish meal diets on growth performance and oxidative status of European sea bass (Dicentrarchus labrax) juveniles","volume":"479","author":"Coutinho","year":"2017","journal-title":"Aquaculture"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1111\/anu.12015","article-title":"Quantitative dietary methionine requirement of juvenile golden pompano Trachinotus ovatus at a constant dietary cystine level","volume":"19","author":"Niu","year":"2013","journal-title":"Aquac. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"114592","DOI":"10.1016\/j.anifeedsci.2020.114592","article-title":"Optimum dietary methionine requirement of juvenile silver pompano, Trachinotus blochii (Lacepede, 1801)","volume":"268","author":"Ebeneezar","year":"2020","journal-title":"Anim. Feed Sci. Technol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"110960","DOI":"10.1016\/j.ygeno.2024.110960","article-title":"Methionine restriction reduced growth performance and exacerbated lipid deposition in hybrid grouper (Epinephelus fuscoguttatus \u2640 \u00d7 E. lanceolatus \u2642) under high-lipid diets by suppressing the lipid degradation pathways with the single-cell RNA-seq analysis","volume":"116","author":"Zhang","year":"2024","journal-title":"Genomics"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"101701","DOI":"10.1016\/j.aqrep.2023.101701","article-title":"Methionine supplemented in a high-fat diet: Effects of growth performance and lipid metabolism in rice field eel (Monopterus albus)","volume":"32","author":"Hu","year":"2023","journal-title":"Aquac. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1016\/j.marpolbul.2010.03.004","article-title":"Seasonal baseline levels of physiological and biochemical parameters in polar cod (Boreogadus saida): Implications for environmental monitoring","volume":"60","author":"Nahrgang","year":"2010","journal-title":"Mar. Pollut. Bull."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Shen, Y., and Lau-Cam, C.A. (2019). Taurine enhances the protective actions of fish oil against D-galactosamine-induced metabolic changes and hepatic lipid accumulation and injury in the rat. Taurine 11, Springer.","DOI":"10.1007\/978-981-13-8023-5_7"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"734632","DOI":"10.1016\/j.aquaculture.2019.734632","article-title":"Dietary supplementation with n-3 high unsaturated fatty acids decreases serum lipid levels and improves flesh quality in the marine teleost golden pompano Trachinotus ovatus","volume":"516","author":"Li","year":"2020","journal-title":"Aquaculture"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.aquaculture.2018.06.002","article-title":"Effect of dietary cholesterol and phospholipids on feed intake, growth performance and cholesterol metabolism in juvenile turbot (Scophthalmus maximus L.)","volume":"495","author":"Zhu","year":"2018","journal-title":"Aquaculture"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.aquaculture.2014.03.027","article-title":"Feed intake, growth performance and cholesterol metabolism in juvenile turbot (Scophthalmus maximus L.) fed defatted fish meal diets with graded levels of cholesterol","volume":"428\u2013429","author":"Zhu","year":"2014","journal-title":"Aquaculture"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.aquaculture.2011.06.028","article-title":"Effects of dietary cholesterol on growth performance, feed intake and cholesterol metabolism in juvenile turbot (Scophthalmus maximus L.) fed high plant protein diets","volume":"319","author":"Yun","year":"2011","journal-title":"Aquaculture"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1159\/000330072","article-title":"High insulin concentrations repress insulin receptor gene expression in calf hepatocytes cultured in vitro","volume":"27","author":"Zhang","year":"2011","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Xu, J., Yao, X., Li, X., Xie, S., Chi, S., Zhang, S., Cao, J., and Tan, B. (2022). Bile acids reduced the lipid deposition in fatty-degenerated hepatocytes of pearl gentian grouper (Epinephelus fuscoguttatus\u2642 \u00d7 Epinephelus lanceolatus\u2640) in vitro. Front. Mar. Sci., 9.","DOI":"10.3389\/fmars.2022.861117"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Bojarski, B., Witeska, M., and Kondera, E. (2025). Blood biochemical biomarkers in fish toxicology\u2014A review. Animals, 15.","DOI":"10.3390\/ani15070965"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1007\/s10695-017-0359-y","article-title":"Hemolysis interference in measuring fish plasma biochemical indicators","volume":"43","author":"Mirghaed","year":"2017","journal-title":"Fish Physiol. Biochem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1111\/raq.12753","article-title":"Application of haematology parameters for health management in fish farms","volume":"15","author":"Chen","year":"2023","journal-title":"Rev. Aquac."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1016\/j.tcb.2023.05.003","article-title":"Regulation of ferroptosis by lipid metabolism","volume":"33","author":"Pope","year":"2023","journal-title":"Trends Cell Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"738959","DOI":"10.1016\/j.aquaculture.2022.738959","article-title":"Transcriptome analysis reveals the effects of dietary lipid level on growth performance and immune response in golden pompano (Trachinotus ovatus)","volume":"563","author":"Song","year":"2023","journal-title":"Aquaculture"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/j.ijbiomac.2020.06.085","article-title":"Transcription factor ppar\u03b1b activates fads2s to promote LC-PUFA biosynthesis in the golden pompano Trachinotus ovatus (Linnaeus 1758)","volume":"161","author":"Zhu","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"738532","DOI":"10.1016\/j.aquaculture.2022.738532","article-title":"Differential regulatory effects of optimal or excessive dietary lipid levels on growth, lipid metabolism and physiological response in black seabream (Acanthopagrus schlegelii)","volume":"560","author":"Shen","year":"2022","journal-title":"Aquaculture"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"9555855","DOI":"10.1155\/2023\/9555855","article-title":"Effects of genistein on lipid metabolism, antioxidant activity, and immunity of common carp (Cyprinus carpio L.) fed with high-carbohydrate and high-fat diets","volume":"2023","author":"Yang","year":"2023","journal-title":"Aquac. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.cbpb.2019.01.005","article-title":"Dietary methionine increased the lipid accumulation in juvenile tiger puffer Takifugu rubripes","volume":"230","author":"Xu","year":"2019","journal-title":"Comp. Biochem. Physiol. Part B Biochem. Mol. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"8592097","DOI":"10.1155\/anu\/8592097","article-title":"Methionine promoted the growth of golden pompano by activating intestinal transport and TOR signaling pathway","volume":"2025","author":"Song","year":"2025","journal-title":"Aquac. Nutr."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"737690","DOI":"10.1016\/j.aquaculture.2021.737690","article-title":"Evaluation of four macro-algae on growth performance, anti-oxidant capacity and non-specific immunity in golden pompano (Trachinotus ovatus)","volume":"548","author":"Xie","year":"2022","journal-title":"Aquaculture"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.redox.2016.07.001","article-title":"Dual NRF2 paralogs in Coho salmon and their antioxidant response element targets","volume":"9","author":"Ramsden","year":"2016","journal-title":"Redox Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3221","DOI":"10.1007\/s00018-016-2223-0","article-title":"Role of Nrf2\/HO-1 system in development, oxidative stress response and diseases: An evolutionarily conserved mechanism","volume":"73","author":"Loboda","year":"2016","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.fsi.2020.07.005","article-title":"The immunoreaction and antioxidant capacity of juvenile blunt snout bream (Megalobrama amblycephala) involves the PI3K\/Akt\/Nrf2 and NF-\u03baB signal pathways in response to dietary methionine levels","volume":"105","author":"Ji","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"102531","DOI":"10.1016\/j.aqrep.2024.102531","article-title":"A study on the function of methionine in growth, immunity, antioxidant, endoplasmic reticulum stress and apoptosis of largemouth bass (Micropterus salmoides)","volume":"39","author":"Liang","year":"2024","journal-title":"Aquac. Rep."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.fsi.2016.07.020","article-title":"Methionine hydroxy analogue enhanced fish immunity via modulation of NF-\u03baB, TOR, MLCK, MAPKs and Nrf2 signaling in young grass carp (Ctenopharyngodon idella)","volume":"56","author":"Pan","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Gao, J., Liu, M., Guo, H., Zhu, K., Liu, B., Liu, B., Zhang, N., and Zhang, D. (2022). ROS induced by Streptococcus agalactiae activate inflammatory responses via the TNF-\u03b1\/NF-\u03baB signaling pathway in golden pompano Trachinotus ovatus (Linnaeus, 1758). Antioxidants, 11.","DOI":"10.3390\/antiox11091809"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1111\/anu.13225","article-title":"Dietary lipid levels affected growth performance, lipid accumulation, inflammatory response and apoptosis of Japanese seabass (Lateolabrax japonicus)","volume":"27","author":"Xie","year":"2021","journal-title":"Aquac. Nutr."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1016\/j.cell.2012.03.042","article-title":"Ferroptosis: An iron-dependent form of nonapoptotic cell death","volume":"149","author":"Dixon","year":"2012","journal-title":"Cell"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Duan, Y., Xiao, M., Zhu, R., Nan, Y., Yang, Y., Huang, X., and Zhang, D. (2025). Effects of ammonia stress on the antioxidant, ferroptosis, and immune response in the liver of golden pompano Trachinotus ovatus. Antioxidants, 14.","DOI":"10.3390\/antiox14040419"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1038\/s41580-024-00703-5","article-title":"The cell biology of ferroptosis","volume":"25","author":"Dixon","year":"2024","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s00011-022-01672-1","article-title":"Molecular mechanisms of ferroptosis and relevance to inflammation","volume":"72","author":"Deng","year":"2023","journal-title":"Inflamm. Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"177534","DOI":"10.1016\/j.scitotenv.2024.177534","article-title":"A comprehensive review of ferroptosis in environmental pollutants-induced chronic obstructive pulmonary disease","volume":"957","author":"Jiang","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"106437","DOI":"10.1016\/j.jff.2024.106437","article-title":"Astaxanthin attenuates doxorubicin-induced liver injury via suppression of ferroptosis in rats","volume":"121","author":"Yin","year":"2024","journal-title":"J. Funct. Foods"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"107038","DOI":"10.1016\/j.envint.2021.107038","article-title":"Glyphosate damages blood-testis barrier via NOX1-triggered oxidative stress in rats: Long-term exposure as a potential risk for male reproductive health","volume":"159","author":"Liu","year":"2022","journal-title":"Environ. Int."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2494S","DOI":"10.1093\/jn\/nxaa094","article-title":"Metabolism of sulfur-containing amino acids: How the body copes with excess methionine, cysteine, and sulfide","volume":"150","author":"Stipanuk","year":"2020","journal-title":"J. Nutr."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/bph.14446","article-title":"Regulators of the transsulfuration pathway","volume":"176","author":"Sbodio","year":"2019","journal-title":"Br. J. Pharmacol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.freeradbiomed.2017.07.010","article-title":"Glutathione peroxidase 4-catalyzed reduction of lipid hydroperoxides in membranes: The polar head of membrane phospholipids binds the enzyme and addresses the fatty acid hydroperoxide group toward the redox center","volume":"112","author":"Cozza","year":"2017","journal-title":"Free Radic. Biol. Med."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.freeradbiomed.2018.09.014","article-title":"Role of GPX4 in ferroptosis and its pharmacological implication","volume":"133","author":"Seibt","year":"2019","journal-title":"Free Radic. Biol. Med."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"114889","DOI":"10.1016\/j.ecoenv.2023.114889","article-title":"Methionine alleviates heat stress-induced ferroptosis in bovine mammary epithelial cells through the Nrf2 pathway","volume":"256","author":"Xu","year":"2023","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Homma, T., Kobayashi, S., and Fujii, J. (2022). Methionine deprivation reveals the pivotal roles of cell cycle progression in ferroptosis that is induced by cysteine starvation. Cells, 11.","DOI":"10.3390\/cells11101603"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/s40104-017-0194-0","article-title":"The relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (Ctenopharyngodon idella)","volume":"8","author":"Wu","year":"2017","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"125627","DOI":"10.1016\/j.envpol.2024.125627","article-title":"Aquatic high iron induces hepatic ferroptosis in zebrafish (Danio rerio) via interleukin-22 signaling pathway","volume":"367","author":"Feng","year":"2025","journal-title":"Environ. Pollut."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"2538S","DOI":"10.1093\/jn\/nxaa254","article-title":"Metabolic consequences of supplemented methionine in a clinical context","volume":"150","author":"Engelen","year":"2020","journal-title":"J. Nutr."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"5706177","DOI":"10.1155\/2023\/5706177","article-title":"Methionine: An indispensable amino acid in cellular metabolism and health of Atlantic salmon","volume":"2023","author":"Espe","year":"2023","journal-title":"Aquac. Nutr."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"8971","DOI":"10.1038\/s41467-024-53380-5","article-title":"Methionine-SAM metabolism-dependent ubiquinone synthesis is crucial for ROS accumulation in ferroptosis induction","volume":"15","author":"Xia","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.placenta.2025.04.016","article-title":"Homocysteine induces endometrial ferroptosis via MAPK pathway in recurrent pregnancy loss","volume":"165","author":"Yang","year":"2025","journal-title":"Placenta"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.cell.2017.09.021","article-title":"Ferroptosis: A regulated cell death nexus linking metabolism, redox biology, and disease","volume":"171","author":"Stockwell","year":"2017","journal-title":"Cell"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.freeradbiomed.2020.02.027","article-title":"Lipid peroxidation and ferroptosis: The role of GSH and GPx4","volume":"152","author":"Ursini","year":"2020","journal-title":"Free Radic. Biol. Med."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.tcb.2015.10.014","article-title":"Ferroptosis: Death by lipid peroxidation","volume":"26","author":"Yang","year":"2016","journal-title":"Trends Cell Biol."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/7\/873\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T05:34:30Z","timestamp":1784007270000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/7\/873"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,14]]},"references-count":85,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["antiox15070873"],"URL":"https:\/\/doi.org\/10.3390\/antiox15070873","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,14]]}}}