{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T04:47:22Z","timestamp":1785646042473,"version":"3.56.0"},"reference-count":39,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32172792"],"award-info":[{"award-number":["32172792"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32372943"],"award-info":[{"award-number":["32372943"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["32172792"],"award-info":[{"award-number":["32172792"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["32372943"],"award-info":[{"award-number":["32372943"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS-44-KXJ7"],"award-info":[{"award-number":["CARS-44-KXJ7"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]},{"award":["CARS-44-KXJ7"],"award-info":[{"award-number":["CARS-44-KXJ7"]}],"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Master Supervisor Team Fund of Fujian Agriculture and Forestry University"},{"id":[{"id":"https:\/\/ror.org\/04kx2sy84","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Special Fund for Science and Technology Innovation of Fujian Agriculture and Forestry University","award":["KFb22060XA"],"award-info":[{"award-number":["KFb22060XA"]}]},{"award":["KFb22060XA"],"award-info":[{"award-number":["KFb22060XA"]}],"id":[{"id":"https:\/\/ror.org\/04kx2sy84","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Long non-coding RNAs (lncRNAs) participate in insect immune regulation, but their relationship with antioxidant responses during fungal infection remains unclear. Here, we examined a candidate regulatory relationship among lncRNA1386.1, novel-miR0032-5p, and the catalase gene (CAT) in Apis cerana worker larvae infected with Ascosphaera apis. Dual-luciferase reporter assays showed that M-miR0032-5p reduced the activity of reporters containing the predicted miRNA response element within lncRNA1386.1 or CAT, whereas mutation of these sequences weakened or abolished the response. In infected larvae, lncRNA1386.1 silencing and novel-miR0032-5p overexpression reduced CAT transcript abundance and CAT protein concentration, and increased dihydroethidium (DHE) fluorescence intensity. These treatments were also accompanied by alterations in the transcript abundance of the host genes Dorsal1 and Relish and the fungal genes Chit3 and STE11-like. Novel-miR0032-5p inhibition generally produced opposite changes in CAT-related measurements, DHE fluorescence intensity, and the selected host and fungal transcripts. lncRNA1386.1 silencing was associated with increased larval survival, whereas novel-miR0032-5p inhibition was associated with a higher hazard of first visible external mycelial growth without significantly affecting survival. These findings support a negative regulatory role of novel-miR0032-5p in CAT expression and indicate that lncRNA1386.1 participates in a candidate shared miRNA-responsive regulatory relationship. This candidate regulatory relationship was associated with CAT-related antioxidant regulation, superoxide-associated DHE fluorescence, immune-related transcription, selected fungal transcriptional responses, and visible external mycelial growth during A. apis infection.<\/jats:p>","DOI":"10.3390\/antiox15080959","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T13:23:20Z","timestamp":1785504200000},"page":"959","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["lncRNA1386.1\/novel-miR0032-5p Axis Targets CAT Gene to Modulate Redox and Immune Reponses of Apis cerana Larvae to Ascosphaera apis Infection"],"prefix":"10.3390","volume":"15","author":[{"given":"Xiaoxue","family":"Fan","sequence":"first","affiliation":[{"name":"College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"National & Local United Engineering Laboratory of Natural Biotoxin, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Apitherapy Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nian","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunze","family":"Li","sequence":"additional","affiliation":[{"name":"College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiyao","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"National & Local United Engineering Laboratory of Natural Biotoxin, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Apitherapy Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiarun","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4262-9013","authenticated-orcid":false,"given":"Jianfeng","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"National & Local United Engineering Laboratory of Natural Biotoxin, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Apitherapy Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingwei","family":"Tan","sequence":"additional","affiliation":[{"name":"College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"National & Local United Engineering Laboratory of Natural Biotoxin, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Apitherapy Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dafu","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"National & Local United Engineering Laboratory of Natural Biotoxin, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Apitherapy Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"National & Local United Engineering Laboratory of Natural Biotoxin, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Apitherapy Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"558","DOI":"10.3390\/insects4040558","article-title":"Ecology, behaviour and control of Apis cerana with a focus on relevance to the Australian incursion","volume":"4","author":"Koetz","year":"2013","journal-title":"Insects"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1080\/00218839.2018.1475943","article-title":"Morphological and molecular identification of chalkbrood disease pathogen Ascosphaera apis in Apis cerana cerana","volume":"57","author":"Chen","year":"2018","journal-title":"J. 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