{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T04:28:21Z","timestamp":1787113701739,"version":"build-2736575974"},"reference-count":64,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Jiangsu Natural Science Foundation","doi-asserted-by":"crossref","award":["BK20181138"],"award-info":[{"award-number":["BK20181138"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The Taiwan loach (Paramisgurnus dabryanus ssp. Taiwan) is a popular cultured fish in southern China due to its rich nutritional content and rapid growth. Oxidative stress and homeostatic imbalance induced by low temperature are among the key factors restricting its large-scale aquaculture. Therefore, it is of great significance to investigate the oxidative stress damage and the adaptive mechanisms employed by this species in response to low temperature. In this study, the water temperature was lowered from 24 \u00b0C to 8 \u00b0C at a rate of 2 \u00b0C\/h. Afterwards, the temperature was held at 8 \u00b0C for two durations: 12 h and 48 h. Antioxidant indices indicated that the Taiwan loach suffered from oxidative stress damage at the 12 h stage, but the antioxidant enzyme defense system was not fully activated. As the cold stress extended to 48 h, the activities of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) increased significantly (p &lt; 0.01). Histological observations revealed that the livers exhibited cellular vacuolation, sinusoid congestion and karyolysis under cold stress. Transcriptomic data revealed that the Taiwan loach underwent adaptive alterations in response to low temperature through diverse pathways. We speculate that, at low temperature, the Taiwan loach may, on the one hand, regulate lipid metabolism to maintain cell membrane fluidity and meet energy demands, and, on the other hand, reprogram protein synthesis and processing to avert the overaccumulation of misfolded proteins. In addition, it also adopts a strategy of lysine and polyamine accumulation. This study provides a novel theoretical basis for breeding cold-tolerant varieties of Taiwan loach and optimizing overwintering aquaculture management.<\/jats:p>","DOI":"10.3390\/antiox15081022","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T08:05:41Z","timestamp":1786953941000},"page":"1022","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Physiological and Transcriptomic Responses to Cold Stress in Taiwan Loach (Paramisgurnus dabryanus ssp. Taiwan)"],"prefix":"10.3390","volume":"15","author":[{"given":"Wei","family":"Zhou","sequence":"first","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"},{"name":"Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiale","family":"Chen","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"},{"name":"Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4408-9150","authenticated-orcid":false,"given":"Yacheng","family":"Hu","sequence":"additional","affiliation":[{"name":"Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang 443100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dezhi","family":"Li","sequence":"additional","affiliation":[{"name":"College of Fisheries and Life Sciences, Dalian Ocean University, Dalian 116023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tengfei","family":"Yu","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"},{"name":"Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaishun","family":"Shen","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"},{"name":"Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1111\/jwas.12853","article-title":"Responses of aquaculture fish to climate change-induced extreme temperatures: A review","volume":"53","author":"Islam","year":"2022","journal-title":"J. World Aquac. Soc."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Menon, S.V., Kumar, A., Middha, S.K., Paital, B., Mathur, S., Johnson, R., Kademan, A., Usha, T., Hemavathi, K., and Dayal, S. (2023). Water physicochemical factors and oxidative stress physiology in fish, a review. Front. Environ. Sci., 11.","DOI":"10.3389\/fenvs.2023.1240813"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"110078","DOI":"10.1016\/j.fsi.2024.110078","article-title":"Effects of chronic cold stress and thermal stress on growth performance, hepatic apoptosis, oxidative stress, immune response and gut microbiota of juvenile hybrid sturgeon (Acipenser baerii \u2640 \u00d7 A. schrenkii \u2642)","volume":"157","author":"Liu","year":"2025","journal-title":"Fish Shellfish Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Chen, J., Li, H., Xu, P., Tang, Y., Su, S., Liu, G., Wu, N., Xue, M., Yu, F., and Feng, W. (2022). Hypothermia-mediated apoptosis and inflammation contribute to antioxidant and immune adaption in freshwater drum, Aplodinotus grunniens. Antioxidants, 11.","DOI":"10.3390\/antiox11091657"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Jin, M., Zheng, A., Mkulo, E.M., Wang, L., Zhang, H., Tang, B., Zhou, H., Wang, B., Huang, J., and Wang, Z. (2025). Metabolomics-based analysis of adaptive mechanism of Eleutheronema tetradactylum to low-temperature stress. Animals, 15.","DOI":"10.3390\/ani15081174"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"bbab563","DOI":"10.1093\/bib\/bbab563","article-title":"A simple guide to de novo transcriptome assembly and annotation","volume":"23","author":"Raghavan","year":"2022","journal-title":"Brief. Bioinform."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"100657","DOI":"10.1016\/j.aqrep.2021.100657","article-title":"Comparative transcriptomic analysis brings new insights into the response to acute temperature acclimation in burbot (Lota lota lota)","volume":"20","author":"Yang","year":"2021","journal-title":"Aquac. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Liu, L., Zhang, R., Wang, X., Zhu, H., and Tian, Z. (2020). Transcriptome analysis reveals molecular mechanisms responsive to acute cold stress in the tropical stenothermal fish tiger barb (Puntius tetrazona). BMC Genom., 21.","DOI":"10.1186\/s12864-020-07139-z"},{"key":"ref_9","first-page":"101358","article-title":"Investigation of cold adaptation mechanisms by transcriptome analysis in the liver of yellowtail kingfish (Seriola aureovittata)","volume":"52","author":"Cao","year":"2024","journal-title":"Comp. Biochem. Physiol. Part D Genom. Proteom."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"116835","DOI":"10.1016\/j.ecoenv.2024.116835","article-title":"Transcriptional dynamic changes in energy metabolism, protein synthesis and cell cycle regulation reveal the biological adaptation mechanisms of juvenile Acrossocheilus wenchowensis under acute temperature changes","volume":"283","author":"He","year":"2024","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_11","unstructured":"Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, National Fisheries Technology Extension Center, and China Society of Fisheries (2025). China Fishery Statistical Yearbook 2025, (In Chinese)."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"736979","DOI":"10.1016\/j.aquaculture.2021.736979","article-title":"Comparative transcriptome analysis of the molecular mechanism underlying the golden red colour in mutant Taiwanese loach","volume":"543","author":"Huang","year":"2021","journal-title":"Aquaculture"},{"key":"ref_13","first-page":"1","article-title":"Comparative study on body index, nutrient composition, and digestive enzyme activity of Misgurnus anguillicaudatus, Paramisgurnus dabryanus, and Paramisgurnus dabryanus ssp","volume":"74","author":"Mao","year":"2022","journal-title":"Isr. J. Aquac. Bamidgeh"},{"key":"ref_14","unstructured":"Li, J., Hu, Q., Xia, Y., Zhan, D., and Li, Y. (2024). Indoor Factory Breeding Experiment of Paramisgurnus dabryanus. China Fish., 65\u201366. (In Chinese)."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e70026","DOI":"10.1111\/fwb.70026","article-title":"Asian loaches: An emerging threat as global invaders","volume":"70","author":"Haubrock","year":"2025","journal-title":"Freshw. Biol."},{"key":"ref_16","unstructured":"Tao, Z., Ma, B., Wang, H., Li, Q., and Xu, X. (2020). The Effects of Cold Stress on Antioxidant Defense and Energy Metabolism of Paramisgurnus Dabryanus. Jiangxi Fish. Sci. Technol., 3\u20138. (In Chinese)."},{"key":"ref_17","first-page":"58","article-title":"Effects of Temperature Stress on the Partial Antioxidative and Non-Specific Immunity Indices of Paramisgurnus dabryanus","volume":"40","author":"Fan","year":"2019","journal-title":"Prog. Fish. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Refaey, M.M., Mehrim, A.I., El-Komy, M.M., Zenhom, O.A., and Mansour, A.T. (2023). Chronic cold-stress induced histopathological changes, oxidative stress, and alterations in liver functions and nutrient composition of hybrid red tilapia and the potential protection of unsaturated fatty acids. Front. Mar. Sci., 10.","DOI":"10.3389\/fmars.2023.1148978"},{"key":"ref_19","unstructured":"Yu, J. (2022). Adaptive Regulation of Larimichthys crocea Under LowTemperature and Rapids Stress. [Master\u2019s Thesis, Shanghai Ocean University]. (In Chinese)."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-Seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_21","first-page":"410","article-title":"Corset: Enabling differential gene expression analysis for de novo assembled transcriptomes","volume":"15","author":"Davidson","year":"2014","journal-title":"Genome Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1111\/jfb.15037","article-title":"An updated review of cold shock and cold stress in fish","volume":"100","author":"Reid","year":"2022","journal-title":"J. Fish Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"740200","DOI":"10.1016\/j.aquaculture.2023.740200","article-title":"Integration of transcriptome and metabolome reveals molecular mechanisms responsive to cold stress in gynogenetic mrigal carp (Cirrhinus mrigala)","volume":"579","author":"Li","year":"2024","journal-title":"Aquaculture"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"736277","DOI":"10.1016\/j.aquaculture.2020.736277","article-title":"Effects of heat stress on histopathology, antioxidant enzymes, and transcriptomic profiles in gills of pikeperch Sander lucioperca","volume":"534","author":"Chen","year":"2021","journal-title":"Aquaculture"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Saxena, A., Lakshmi, J., Bhattacharjya, R., Singh, P.K., Mishra, B., and Tiwari, A. (2023). The role of antioxidant enzymes in diatoms and their therapeutic role. Marine Antioxidants, Elsevier.","DOI":"10.1016\/B978-0-323-95086-2.00019-9"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Sun, Y., Fu, Z., Liu, X., and Ma, Z. (2024). The Impact of Acute ammonia nitrogen stress on the gill tissue structure and antioxidant ability of gills and red and white muscle in juvenile yellowfin tuna (Thunnus albacares). Antioxidants, 13.","DOI":"10.3390\/antiox13111357"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"172318","DOI":"10.1016\/j.scitotenv.2024.172318","article-title":"Comprehensive analysis of histophysiology, transcriptome and metabolome tolerance mechanisms in black porgy (Acanthopagrus schlegelii) under low temperature stress","volume":"927","author":"Wang","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_28","first-page":"3","article-title":"Apoptosis, oncosis, and necrosis. An overview of cell death","volume":"146","author":"Majno","year":"1995","journal-title":"Am. J. Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"738543","DOI":"10.1016\/j.aquaculture.2022.738543","article-title":"Comparative transcriptomes and metabolomes reveal different tolerance mechanisms to cold stress in two different catfish species","volume":"560","author":"Liu","year":"2022","journal-title":"Aquaculture"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Wu, N., Wen, H., Xu, P., Chen, J., Xue, M., Li, J., Wang, M., Song, C., and Li, H. (2023). PPAR signaling maintains metabolic homeostasis under hypothermia in freshwater drum (Aplodinotus grunniens). Metabolites, 13.","DOI":"10.3390\/metabo13010102"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"741103","DOI":"10.1016\/j.aquaculture.2024.741103","article-title":"An integrated analysis of transcriptome and metabolome to reveal the effects of temperature stress on energy metabolism and physiological responses in Schizothorax wangchiachii muscles","volume":"591","author":"Zhao","year":"2024","journal-title":"Aquaculture"},{"key":"ref_32","first-page":"80","article-title":"Transcriptional response to low temperature in the yellow drum (Nibea albiflora) and identification of genes related to cold stress","volume":"28","author":"Xu","year":"2018","journal-title":"Comp. Biochem. Physiol. Part D Genom. Proteom."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Wu, G., Baumeister, R., and Heimbucher, T. (2023). Molecular mechanisms of lipid-based metabolic adaptation strategies in response to cold. Cells, 12.","DOI":"10.3390\/cells12101353"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Miao, B.-B., Niu, S.-F., Wu, R.-X., Liang, Z.-B., and Zhai, Y. (2022). The microRNAs-transcription factors-mRNA regulatory network plays an important role in resistance to cold stress in the pearl gentian grouper. Front. Mar. Sci., 8.","DOI":"10.3389\/fmars.2021.824533"},{"key":"ref_35","first-page":"123","article-title":"Effects of Low Temperature Stress on Physiology and Biochemistry of Lipid Metabolism of Juvenile Cobia, Rachycentron canadum","volume":"41","author":"Cai","year":"2021","journal-title":"J. Guangdong Ocean Univ."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.fsi.2022.01.017","article-title":"Transcriptome, antioxidant enzymes and histological analysis reveal molecular mechanisms responsive to long-term cold stress in silver pomfret (Pampus argenteus)","volume":"121","author":"Zhang","year":"2022","journal-title":"Fish Shellfish Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Guo, Z., Chi, R., Peng, Y., Sun, K., Liu, H., Guo, F., and Guo, J. (2024). The role and interactive mechanism of endoplasmic reticulum stress and ferroptosis in musculoskeletal disorders. Biomolecules, 14.","DOI":"10.3390\/biom14111369"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"742886","DOI":"10.1016\/j.aquaculture.2025.742886","article-title":"Molecular mechanisms of cold stress-induced energy stress, cholesterol metabolic disorders, and apoptosis in Pangasianodon hypophthalmus","volume":"610","author":"Wang","year":"2026","journal-title":"Aquaculture"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"102847","DOI":"10.1016\/j.aqrep.2025.102847","article-title":"Integration of histology, transcriptome and metabolome reveals the effect of cold stress on the liver of cobia (Rachycentron canadum)","volume":"42","author":"Zhao","year":"2025","journal-title":"Aquac. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"6869","DOI":"10.1038\/s41467-021-27244-1","article-title":"The structural basis for the phospholipid remodeling by lysophosphatidylcholine acyltransferase 3","volume":"12","author":"Zhang","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"101470","DOI":"10.1016\/j.jbc.2021.101470","article-title":"Update and nomenclature proposal for mammalian lysophospholipid acyltransferases, which create membrane phospholipid diversity","volume":"298","author":"Valentine","year":"2022","journal-title":"J. Biol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.cryobiol.2022.03.004","article-title":"Insect cold-tolerance and lipidome: Membrane lipid composition of two chironomid species differently adapted to cold","volume":"106","author":"Trenti","year":"2022","journal-title":"Cryobiology"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2400006","DOI":"10.1002\/adbi.202400006","article-title":"Spliceosomal snRNAs, the Essential Players in pre-mRNA Processing in Eukaryotic Nucleus: From Biogenesis to Functions and Spatiotemporal Characteristics","volume":"8","author":"Su","year":"2024","journal-title":"Adv. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1080\/15476286.2022.2100971","article-title":"Principles and correction of 5\u2032-splice site selection","volume":"19","author":"Malard","year":"2022","journal-title":"RNA Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"102042","DOI":"10.1016\/j.aqrep.2024.102042","article-title":"Integrated analysis of transcriptomic and physiological responses to cold stress in Macrobrachium rosenbergii","volume":"36","author":"Yu","year":"2024","journal-title":"Aquac. Rep."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"6580","DOI":"10.1038\/s41467-023-42324-0","article-title":"The SMN complex drives structural changes in human snRNAs to enable snRNP assembly","volume":"14","author":"Prusty","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1261\/rna.2030510","article-title":"Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing","volume":"16","author":"Lardelli","year":"2010","journal-title":"Rna"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"8319","DOI":"10.1038\/s41467-025-63911-3","article-title":"Stabilizing a mammalian RNA thermometer confers neuroprotection in subarachnoid hemorrhage","volume":"16","author":"Zhang","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1042\/BCJ20230410","article-title":"Thermoregulated transcriptomics: The molecular basis and biological significance of temperature-dependent alternative splicing","volume":"481","author":"Haltenhof","year":"2024","journal-title":"Biochem. J."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Liu, S., Tian, F., Qi, D., Qi, H., Wang, Y., Xu, S., and Zhao, K. (2023). Physiological, metabolomic, and transcriptomic reveal metabolic pathway alterations in Gymnocypris przewalskii due to cold exposure. BMC Genom., 24.","DOI":"10.1186\/s12864-023-09587-9"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Sun, Z., Huang, L., Kong, Y., Wang, L., and Kang, B. (2022). Regulating strategies of transcription and alternative splicing for cold tolerance Harpadon nehereus fish. Front. Ecol. Evol., 10.","DOI":"10.3389\/fevo.2022.912113"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1146\/annurev-biochem-071921-122857","article-title":"The structural dynamics of translation","volume":"91","author":"Korostelev","year":"2022","journal-title":"Annu. Rev. Biochem."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Long, Y., Song, G., Yan, J., He, X., Li, Q., and Cui, Z. (2013). Transcriptomic characterization of cold acclimation in larval zebrafish. BMC Genom., 14.","DOI":"10.1186\/1471-2164-14-612"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.cryobiol.2020.06.014","article-title":"Transcriptome analysis of the Larimichthys polyactis under heat and cold stress","volume":"96","author":"Chu","year":"2020","journal-title":"Cryobiology"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Mininni, A.N., Milan, M., Ferraresso, S., Petochi, T., Di Marco, P., Marino, G., Livi, S., Romualdi, C., Bargelloni, L., and Patarnello, T. (2014). Liver transcriptome analysis in gilthead sea bream upon exposure to low temperature. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-765"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"jcs217216","DOI":"10.1242\/jcs.217216","article-title":"The unfolded protein response in metazoan development","volume":"132","author":"Mitra","year":"2019","journal-title":"J. Cell Sci."},{"key":"ref_57","first-page":"001276","article-title":"Endoplasmic reticulum and inner nuclear membrane ubiquitin-conjugating enzymes Ubc6 and Ubc7 confer resistance to hygromycin B in Saccharomyces cerevisiae","volume":"2024","author":"Owutey","year":"2024","journal-title":"Micropublication Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1242\/jcs.189332","article-title":"Cdc48 and Ubx1 participate in a pathway associated with the inner nuclear membrane that governs Asi1 degradation","volume":"129","author":"Pantazopoulou","year":"2016","journal-title":"J. Cell Sci."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Liu, Z., Zhu, L., Wang, X., Liu, S., Ma, A., Chang, H., Sun, Z., Xu, F., and Zhao, H. (2022). Application of transcriptome analysis to investigate the effects of long-term low temperature stress on liver function in the tiger puffer (Takifugu rubripes). Front. Mar. Sci., 9.","DOI":"10.3389\/fmars.2022.1069711"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"739573","DOI":"10.1016\/j.aquaculture.2023.739573","article-title":"Acute temperature stresses trigger liver transcriptome and microbial community remodeling in largemouth bass (Micropterus salmoides)","volume":"573","author":"Yu","year":"2023","journal-title":"Aquaculture"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Xu, X., Zhang, G., Chen, Y., Xu, W., Liu, Y., Ji, G., and Xu, H. (2023). Can proline dehydrogenase\u2014A key enzyme involved in proline metabolism\u2014Be a novel target for cancer therapy?. Front. Oncol., 13.","DOI":"10.3389\/fonc.2023.1254439"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Patriarca, E.J., Cermola, F., D\u2019Aniello, C., Fico, A., Guardiola, O., De Cesare, D., and Minchiotti, G. (2021). The multifaceted roles of proline in cell behavior. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.728576"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"103648","DOI":"10.1016\/j.redox.2025.103648","article-title":"Defence Warriors: Exploring the crosstalk between polyamines and oxidative stress during microbial pathogenesis","volume":"83","author":"Nair","year":"2025","journal-title":"Redox Biol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s00018-021-04043-x","article-title":"Acute hypoxia elevates arginase 2 and induces polyamine stress response in zebrafish via evolutionarily conserved mechanism","volume":"79","author":"Banerjee","year":"2022","journal-title":"Cell. Mol. Life Sci."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/8\/1022\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T04:23:31Z","timestamp":1787113411000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/8\/1022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":64,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["antiox15081022"],"URL":"https:\/\/doi.org\/10.3390\/antiox15081022","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]}}}