{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T16:43:09Z","timestamp":1787330589421,"version":"build-2736575974"},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Professional Technical Service Platform","award":["21DZ2292200"],"award-info":[{"award-number":["21DZ2292200"]}]},{"name":"Agri-food Storage and Processing Engineering Technology Research Center of Shanghai","award":["19DZ2251600"],"award-info":[{"award-number":["19DZ2251600"]}]},{"name":"Shanghai Agri cultural Science and Technology Innovation Program","award":["I2023007"],"award-info":[{"award-number":["I2023007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Differences in the composition, microstructure, infrared spectral characteristics, and enzymatic hydrolysis properties of rice bran protein extracted by four methods were investigated, and the antioxidant and anti-photoaging activities of peptides derived from the resulting hydrolysates were further evaluated. Rice bran protein obtained via ultrasonic pretreatment combined with alkaline solublilization and acid precipitation (URP) exhibited relatively high purity (59.17%) and extraction yield (47.61%), together with increased surface porosity, enhanced hydration capacity, and improved enzymatic hydrolysis performance. The URP hydrolysate (URPP) showed a protein content of 81.00%, a degree of hydrolysis of 33.57%, and marked antioxidant activity (ABTS, 684.21; ORAC, 2016.15 \u03bcmol TE\/g sample). The identified peptides were predominantly short and enriched in hydrophobic amino acids. Structural analysis suggested that the indole N-H group of tryptophan may play an important role in the antioxidant activity of these peptides. Moreover, these peptides alleviated UVB-induced photoaging in HaCaT cells by reducing oxidative stress and inflammatory responses and downregulating the mRNA expressions of AP-1, MMP-1 and MMP-3. Overall, these findings reveal an association between the extraction method, structural characteristics, and enzymatic hydrolysis properties of rice bran protein and the biological activities of its derived peptides, providing a basis for the high-value utilization of rice bran protein and the development of antioxidant and anti-photoaging functional ingredients.<\/jats:p>","DOI":"10.3390\/antiox15081049","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T15:51:05Z","timestamp":1787327465000},"page":"1049","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rice Bran Proteins Extracted by Different Methods: Structural Properties and Antioxidant and Anti-Photoaging Activities of Their Hydrolysates"],"prefix":"10.3390","volume":"15","author":[{"given":"Xiao","family":"Wang","sequence":"first","affiliation":[{"name":"Crop Breeding and Cultivation Research Institution, Research 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Products Preservation and Processing, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1350-1890","authenticated-orcid":false,"given":"Jinglin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Crop Breeding and Cultivation Research Institution, Research Center for Agricultural Products Preservation and Processing, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"He","sequence":"additional","affiliation":[{"name":"Crop Breeding and Cultivation Research Institution, Research Center for Agricultural Products Preservation and Processing, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxia","family":"Liu","sequence":"additional","affiliation":[{"name":"Crop Breeding and Cultivation 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jucai","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2374","DOI":"10.1007\/s11694-022-01796-2","article-title":"Physicochemical and antioxidant properties of rice bran protein hydrolysates obtained from different proteases","volume":"17","author":"Onsaard","year":"2023","journal-title":"J. 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