{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T18:22:34Z","timestamp":1787595754840,"version":"build-2736575974"},"reference-count":50,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Agriculture Research System"},{"id":[{"id":"https:\/\/ror.org\/03p5eky76","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Shanghai Agricultural Products Preservation and Processing Technical Service Platform","award":["21DZ2292200"],"award-info":[{"award-number":["21DZ2292200"]}]},{"name":"Shanghai Agri-food Storage and Processing Engineering Technology Research Center","award":["19DZ2251600"],"award-info":[{"award-number":["19DZ2251600"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Fourteen novel antioxidant peptides were screened from the enzymatic hydrolysates of Asian swamp eel bone (ASEB) through an in silico analysis. Their ABTS and ORAC radical scavenging capacities were 1.70\u20134.29-fold and 2.79\u20135.90-fold higher than those of Trolox, respectively. Additionally, two novel peptide sequences (NVGW and WALN) were identified. Quantum chemical calculations combined with active\u2013-site methylation experiments demonstrated that hydrogen atoms on tryptophan\u2019s indole nitrogen, tyrosine\u2019s phenolic hydroxyl, and arginine\u2019s guanidinium group play crucial roles in enhancing ABTS and ORAC activities. Molecular docking further showed stable binding of ASEB peptides to myeloperoxidase (MPO) through hydrogen bonds and electrostatic interactions. Further studies indicated that ASEB alleviated oxidative stress in zebrafish by effectively reducing ROS accumulation, restoring redox homeostasis, and modulating the expression of Keap1\u2013Nrf2 pathway-related antioxidant genes. These results enhance our understanding of the antioxidant properties of ASEB-derived peptides and support the high-value utilization of animal byproducts.<\/jats:p>","DOI":"10.3390\/antiox15091057","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:20:28Z","timestamp":1787592028000},"page":"1057","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and In Silico Selection of Novel Antioxidant Peptides from Asian Swamp Eel Bone: Quantum Chemical Calculations, Molecular Docking, and Zebrafish Model Validation"],"prefix":"10.3390","volume":"15","author":[{"given":"Xiao","family":"Wang","sequence":"first","affiliation":[{"name":"Crop Breeding and Cultivation Research Institution, Research Center for Agricultural Products Preservation and Processing, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5820-547X","authenticated-orcid":false,"given":"Jianan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hunan Key Laboratory of Forestry Edible Sources Safety and Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingjie","family":"Chen","sequence":"additional","affiliation":[{"name":"Crop Breeding and Cultivation Research Institution, Research Center for Agricultural Products Preservation and Processing, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinlu","family":"Wang","sequence":"additional","affiliation":[{"name":"Crop Breeding and Cultivation Research Institution, Research Center for Agricultural Products Preservation and Processing, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8257-4408","authenticated-orcid":false,"given":"Khushwant S.","family":"Bhullar","sequence":"additional","affiliation":[{"name":"Department of Agricultural Food & Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, Shanghai 201403, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongru","family":"Liu","sequence":"additional","affiliation":[{"name":"Crop Breeding and Cultivation Research Institution, Research Center for Agricultural Products Preservation and Processing, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Products Cold Chain Logistics, Ministry of Agriculture and Rural Affairs, Institute of Agro-Products Processing and Nuclear agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9519-9803","authenticated-orcid":false,"given":"Chenggang","family":"Cai","sequence":"additional","affiliation":[{"name":"College of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Integrated Rice\u2013Fish Farming Ecosystem, Ministry of Agriculture and Rural Affairs, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Krishnamurthy, H.K., Pereira, M., and Rajavelu, I. 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