{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T03:14:28Z","timestamp":1788146068391,"version":"build-2803163510"},"reference-count":49,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yunnan Key Laboratory of Veterinary Etiological Biology","award":["202449CE340019"],"award-info":[{"award-number":["202449CE340019"]}]},{"name":"Wenzhou Science and Technology Commissioner Special Project","award":["XZ2025111"],"award-info":[{"award-number":["XZ2025111"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Oxidative stress is a major consequence of environmental, metabolic, and disease-related challenges in poultry and can compromise cellular integrity and function. Although antioxidant defense mechanisms are broadly conserved across vertebrates, oxidative-stress responses in avian cells remain less well characterized than those in mammalian systems. Superoxide dismutase 1 (SOD1) is a key antioxidant enzyme, but how elevated SOD1 expression influences both oxidative injury and stress-responsive transcription in chicken cells remains incompletely understood. In this study, we established a stable SOD1-overexpressing chicken DF-1 fibroblast cell line and induced acute oxidative stress using hydrogen peroxide (H2O2). Cellular responses were evaluated using CCK-8 viability assays and Hoechst 33342\/propidium iodide staining, followed by RNA sequencing and transcriptomic analyses. SOD1 overexpression markedly improved cell viability under H2O2 challenge and substantially reduced necrotic cell death, whereas its effect on apoptosis-associated changes was comparatively limited. Transcriptomic profiling showed that H2O2 induced extensive transcriptional reprogramming, while SOD1 overexpression reshaped this response by attenuating a subset of stress-inducible gene programs. Trend clustering further identified an H2O2-responsive module associated mainly with basic amino acid transport and stress-related regulatory processes that was less strongly induced in SOD1-overexpressing cells. Collectively, these findings demonstrate that elevated SOD1 expression enhances resistance to acute oxidative injury and modifies stress-responsive transcription in chicken fibroblasts. This study extends our current understanding of SOD1-mediated antioxidant cytoprotection in an avian cellular model and provides a molecular basis for further investigation of oxidative-stress regulation in poultry.<\/jats:p>","DOI":"10.3390\/antiox15091074","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T13:26:03Z","timestamp":1787837163000},"page":"1074","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["SOD1 Overexpression Alleviates H2O2-Induced Oxidative Stress and Reshapes Transcriptomic Responses in Chicken DF-1 Cells"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9212-3508","authenticated-orcid":false,"given":"Yidan","family":"Wang","sequence":"first","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penghao","family":"Wei","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tinghao","family":"Gao","sequence":"additional","affiliation":[{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwei","family":"Li","sequence":"additional","affiliation":[{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caiqin","family":"Deng","sequence":"additional","affiliation":[{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanjing","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science, Wenzhou Vocational College of Science and Technology, Wenzhou 325000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengcai","family":"Zou","sequence":"additional","affiliation":[{"name":"The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3724-0323","authenticated-orcid":false,"given":"Haiyang","family":"Song","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China"},{"name":"College of Animal Science, Wenzhou Vocational College of Science and Technology, Wenzhou 325000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Sies, H. (2020). Oxidative Stress: Concept and Some Practical Aspects. Antioxidants, 9.","DOI":"10.3390\/antiox9090852"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1038\/s41573-021-00233-1","article-title":"Targeting Oxidative Stress in Disease: Promise and Limitations of Antioxidant Therapy","volume":"20","author":"Forman","year":"2021","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/s42255-022-00591-z","article-title":"Guidelines for Measuring Reactive Oxygen Species and Oxidative Damage in Cells and in vivo","volume":"4","author":"Murphy","year":"2022","journal-title":"Nat. Metab."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Surai, P.F., Kochish, I.I., Fisinin, V.I., and Kidd, M.T. (2019). Antioxidant Defence Systems and Oxidative Stress in Poultry Biology: An Update. Antioxidants, 8.","DOI":"10.3390\/antiox8070235"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"104003","DOI":"10.1016\/j.psj.2024.104003","article-title":"Oxidative Stress in Poultry Production","volume":"103","author":"Oke","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Li, Y., Wang, K., and Li, C. (2024). Oxidative Stress in Poultry and the Therapeutic Role of Herbal Medicine in Intestinal Health. Antioxidants, 13.","DOI":"10.3390\/antiox13111375"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1093\/icb\/icz017","article-title":"Does Cellular Metabolism from Primary Fibroblasts and Oxidative Stress in Blood Differ between Mammals and Birds? The (Lack-Thereof) Scaling of Oxidative Stress","volume":"59","author":"Jimenez","year":"2019","journal-title":"Integr. Comp. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"B468","DOI":"10.1093\/gerona\/56.11.B468","article-title":"Exceptional Cellular Resistance to Oxidative Damage in Long-Lived Birds Requires Active Gene Expression","volume":"56","author":"Ogburn","year":"2001","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1242\/jeb.054643","article-title":"Fibroblasts from Long-Lived Bird Species Are Resistant to Multiple Forms of Stress","volume":"214","author":"Harper","year":"2011","journal-title":"J. Exp. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"9215","DOI":"10.1002\/anie.202000451","article-title":"Superoxide Dismutase 1 in Health and Disease: How a Frontline Antioxidant Becomes Neurotoxic","volume":"60","author":"Trist","year":"2020","journal-title":"Angew. Chem. (Int. Ed. Engl.)"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3446","DOI":"10.1038\/ncomms4446","article-title":"Superoxide Dismutase 1 Acts as a Nuclear Transcription Factor to Regulate Oxidative Stress Resistance","volume":"5","author":"Tsang","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.jneuroim.2006.10.003","article-title":"SOD1 Overexpression Alters ROS Production and Reduces Neurotoxic Inflammatory Signaling in Microglial Cells","volume":"182","author":"Dimayuga","year":"2007","journal-title":"J. Neuroimmunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"101760","DOI":"10.1016\/j.redox.2020.101760","article-title":"SOD1 Suppresses Pro-Inflammatory Immune Responses by Protecting against Oxidative Stress in Colitis","volume":"37","author":"Hwang","year":"2020","journal-title":"Redox Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"10998","DOI":"10.3390\/ijms140610998","article-title":"Sod1 Loss Induces Intrinsic Superoxide Accumulation Leading to P53-Mediated Growth Arrest and Apoptosis","volume":"14","author":"Watanabe","year":"2013","journal-title":"Int. J. Mol. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"8359118","DOI":"10.1155\/2022\/8359118","article-title":"Protective Effect of Phloretin against Hydrogen Peroxide-Induced Oxidative Damage by Enhancing Autophagic Flux in DF-1 Cells","volume":"2022","author":"Song","year":"2022","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Xie, L., Xu, Y., Ding, X., Li, K., Liang, S., Li, D., Wang, Y., Fu, A., Yu, W., and Zhan, X. (2023). Selenomethionine Attenuated H2O2-Induced Oxidative Stress and Apoptosis by Nrf2 in Chicken Liver Cells. Antioxidants, 12.","DOI":"10.3390\/antiox12091685"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: An Ultra-Fast All-in-One FASTQ Preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast Gapped-Read Alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A Fast Spliced Aligner with Low Memory Requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie Enables Improved Reconstruction of a Transcriptome from RNA-Seq Reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1038\/nprot.2016.095","article-title":"Transcript-Level Expression Analysis of RNA-Seq Experiments with HISAT, StringTie, and Ballgown","volume":"11","author":"Pertea","year":"2016","journal-title":"Nat. Protoc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nmeth.4197","article-title":"Salmon Provides Fast and Bias-Aware Quantification of Transcript Expression","volume":"14","author":"Patro","year":"2017","journal-title":"Nat. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated Estimation of Fold Change and Dispersion for RNA-Seq Data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene Ontology: Tool for the Unification of Biology","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"D480","DOI":"10.1093\/nar\/gkm882","article-title":"KEGG for Linking Genomes to Life and the Environment","volume":"36","author":"Kanehisa","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"D447","DOI":"10.1093\/nar\/gku1003","article-title":"STRING V10: Protein\u2013Protein Interaction Networks, Integrated over the Tree of Life","volume":"43","author":"Szklarczyk","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction Networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"103141","DOI":"10.1016\/j.psj.2023.103141","article-title":"Heat Stress and Poultry Production: A Comprehensive Review","volume":"102","author":"Oluwagbenga","year":"2023","journal-title":"Poult. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"103313","DOI":"10.1016\/j.psj.2023.103313","article-title":"Impact of Broiler Breeder Hens\u2019 Age and Egg Storage on Egg Quality, Embryonic Development, and Hatching Traits of FUNAAB-Alpha Chickens","volume":"103","author":"Alo","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"108701","DOI":"10.1016\/j.abb.2020.108701","article-title":"SOD1, More than Just an Antioxidant","volume":"697","author":"Eleutherio","year":"2021","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Xu, J., Su, X., Burley, S.K., and Zheng, X.F.S. (2022). Nuclear SOD1 in Growth Control, Oxidative Stress Response, Amyotrophic Lateral Sclerosis, and Cancer. Antioxidants, 11.","DOI":"10.3390\/antiox11020427"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1046\/j.1471-4159.2001.00417.x","article-title":"Effect of Overexpression of Wild-Type and Mutant Cu\/Zn-Superoxide Dismutases on Oxidative Stress and Cell Death Induced by Hydrogen Peroxide, 4-Hydroxynonenal or Serum Deprivation: Potentiation of Injury by ALS-Related Mutant Superoxide Dismutases and Protection by Bcl-2","volume":"78","author":"Lee","year":"2001","journal-title":"J. Neurochem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0167-4889(01)00131-8","article-title":"Necrotic Cell Death by Hydrogen Peroxide in Immortal DF-1 Chicken Embryo Fibroblast Cells Expressing Deregulated MnSOD and Catalase","volume":"1540","author":"Kim","year":"2001","journal-title":"Biochim. ET Biophys. Acta (BBA) Mol. Cell Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jcp.10119","article-title":"Cellular Response to Oxidative Stress: Signaling for Suicide and Survival","volume":"192","author":"Martindale","year":"2002","journal-title":"J. Cell. Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1080\/10715760600632552","article-title":"Turning Point in Apoptosis\/Necrosis Induced by Hydrogen Peroxide","volume":"40","author":"Saito","year":"2006","journal-title":"Free Radic. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1038\/sj.cdd.4401249","article-title":"The Selection between Apoptosis and Necrosis Is Differentially Regulated in Hydrogen Peroxide-Treated and Glutathione-Depleted Human Promonocytic Cells","volume":"10","author":"Troyano","year":"2003","journal-title":"Cell Death Differ."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"25310","DOI":"10.18632\/oncotarget.15813","article-title":"Oxidative Stress-Induced Apoptosis in Granulosa Cells Involves JNK, P53 and Puma","volume":"8","author":"Yang","year":"2017","journal-title":"Oncotarget"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1089\/ars.2020.8074","article-title":"Reactive Oxygen Species Induced P53 Activation: DNA Damage, Redox Signaling, or Both?","volume":"33","author":"Shi","year":"2020","journal-title":"Antioxid. Redox Signal."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"792639","DOI":"10.1155\/2011\/792639","article-title":"Mitogen-Activated Protein Kinases and Reactive Oxygen Species: How Can ROS Activate MAPK Pathways?","volume":"2011","author":"Son","year":"2011","journal-title":"J. Signal Transduct."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1124\/dmd.121.000705","article-title":"Amino Acid Solute Carrier Transporters in Inflammation and Autoimmunity","volume":"50","author":"Sheng","year":"2022","journal-title":"Drug Metab. Dispos."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1042\/BJ20060941","article-title":"A Feedback Transcriptional Mechanism Controls the Level of the Arginine\/Lysine Transporter Cat-1 during Amino Acid Starvation","volume":"402","author":"Lopez","year":"2007","journal-title":"Biochem. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"9700","DOI":"10.1074\/jbc.M115.704254","article-title":"Amino Acid Transport Associated to Cluster of Differentiation 98 Heavy Chain (CD98hc) Is at the Cross-Road of Oxidative Stress and Amino Acid Availability","volume":"291","author":"Bial","year":"2016","journal-title":"J. Biol. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3961","DOI":"10.1096\/fj.201600698R","article-title":"LC3\/GABARAP Family Proteins: Autophagy-(Un)Related Functions","volume":"30","author":"Schaaf","year":"2016","journal-title":"FASEB J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"424","DOI":"10.5483\/BMBRep.2016.49.8.081","article-title":"Role of the Mammalian ATG8\/LC3 Family in Autophagy: Differential and Compensatory Roles in the Spatiotemporal Regulation of Autophagy","volume":"49","author":"Lee","year":"2016","journal-title":"BMB Rep."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1038\/cdd.2014.150","article-title":"Oxidative Stress and Autophagy: The Clash between Damage and Metabolic Needs","volume":"22","author":"Filomeni","year":"2015","journal-title":"Cell Death Differ."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1152\/physrev.00044.2005","article-title":"The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology","volume":"87","author":"Bedard","year":"2007","journal-title":"Physiol. Rev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.cell.2016.02.026","article-title":"BOK Is a Non-Canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation","volume":"165","author":"Llambi","year":"2016","journal-title":"Cell"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1038\/s41418-023-01158-5","article-title":"BCL-G: 20 Years of Research on a Non-Typical Protein from the BCL-2 Family","volume":"30","author":"Hartman","year":"2023","journal-title":"Cell Death Differ."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s40104-021-00565-3","article-title":"Phenolic Compounds as Natural Feed Additives in Poultry and Swine Diets: A Review","volume":"12","author":"Mahfuz","year":"2021","journal-title":"J. Anim. Sci. Biotechnol."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/9\/1074\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T02:43:11Z","timestamp":1788144191000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/9\/1074"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,27]]},"references-count":49,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["antiox15091074"],"URL":"https:\/\/doi.org\/10.3390\/antiox15091074","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,27]]}}}