{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T05:08:27Z","timestamp":1789016907097,"version":"build-2803163510"},"reference-count":53,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Higher Education, Science and Technology","award":["4303\/83\/DT.03.08\/2025"],"award-info":[{"award-number":["4303\/83\/DT.03.08\/2025"]}]},{"name":"Ministry of Higher Education, Science and Technology","award":["3927\/UN6.RKT\/HK.07.00\/2025"],"award-info":[{"award-number":["3927\/UN6.RKT\/HK.07.00\/2025"]}]},{"award":["4303\/83\/DT.03.08\/2025"],"award-info":[{"award-number":["4303\/83\/DT.03.08\/2025"]}],"id":[{"id":"https:\/\/ror.org\/015snc593","id-type":"ROR","asserted-by":"publisher"}]},{"award":["3927\/UN6.RKT\/HK.07.00\/2025"],"award-info":[{"award-number":["3927\/UN6.RKT\/HK.07.00\/2025"]}],"id":[{"id":"https:\/\/ror.org\/015snc593","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The freeze\u2013thaw process in sperm cryopreservation generates reactive oxygen species (ROS) that impair motility, viability, and fertilizing capacity in fish, including the Javaen barb (Systomus rubripinnis), an endemic Indonesian cyprinid of conservation concern. Cryopreservation underpins gene bank development for aquatic genetic resources and supports biodiversity conservation alongside hatchery-based stock enhancement. This study evaluated five natural antioxidants: basil leaf extract (BLE), noni fruit extract (NFE), moringa leaf extract (MLE), sambiloto leaf extract (SLE), and honey\u2013egg yolk (HEY). Each antioxidant was supplemented into the Kurokura\u2019s cryopreservation extender across a graded concentration series (BLE 1\u20134%, NFE and MLE 0.5\u20131.5%, SLE 6\u20139%, and HEY 1\u20132% with a fixed 5% egg yolk) and compared with an antioxidant-free control. Radical scavenging capacity was measured by DPPH assay (IC50: BLE 134.06, MLE 297.04, NFE 422.86, SLE 3010.46 ppm), and post-thaw motility, duration of motility, viability, abnormality, fertilization rate, and ATP bioluminescence were assessed. The most effective doses tested were doses of 1% for BLE, NFE, and MLE, 1.25% for HEY, and 7% for SLE. BLE at 1% gave the best overall profile (motility 85.00 \u00b1 0.88%; duration 7.14 \u00b1 0.38 min; viability 82.00 \u00b1 1.86%; abnormality 18.26 \u00b1 0.16%), significantly exceeding the control (65.00 \u00b1 2.31%; 4.56 \u00b1 0.33 min; 68.00 \u00b1 2.42%; 23.54 \u00b1 0.28%; p &lt; 0.05), whereas SLE sustained the longest motility. All extracts supported fertilization rates above 82% versus 70.08 \u00b1 3.82% in the control, and 1% BLE preserved post-thaw luminescence equivalent to fresh sperm (165,217 vs. 164,087 RLU), interpreted comparatively. These locally available, plant-derived antioxidants offer a low-cost route to germplasm conservation of Javaen barb, strengthening conservation hatchery and broodstock management, sustainable aquaculture production, and food security for inland fisheries communities.<\/jats:p>","DOI":"10.3390\/antiox15091135","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T08:10:22Z","timestamp":1788855022000},"page":"1135","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Natural Antioxidants Enhance Post-Thaw Sperm Quality and Fertilization Performance in Javaen Barb (Systomus rubripinnis)"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4382-8513","authenticated-orcid":false,"given":"Irfan","family":"Zidni","sequence":"first","affiliation":[{"name":"Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang Regency 45363, West Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0181-9336","authenticated-orcid":false,"given":"Abinawanto","family":"Abinawanto","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, West Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Akhmad Taufiq","family":"Mukti","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Jl. 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Soekarno, Surabaya 60115, East Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4269-7689","authenticated-orcid":false,"given":"Aisyah","family":"Aisyah","sequence":"additional","affiliation":[{"name":"Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang Regency 45363, West Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0244-3150","authenticated-orcid":false,"given":"Suci","family":"Lestari","sequence":"additional","affiliation":[{"name":"Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Prof. Dr. Hamka, East Jakarta 13830, Jakarta, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1213-6867","authenticated-orcid":false,"given":"Yuli","family":"Andriani","sequence":"additional","affiliation":[{"name":"Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang Regency 45363, West Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Iskandar","family":"Iskandar","sequence":"additional","affiliation":[{"name":"Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang Regency 45363, West Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Roffi","family":"Grandiosa","sequence":"additional","affiliation":[{"name":"Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang Regency 45363, West Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Atikah","family":"Nurhayati","sequence":"additional","affiliation":[{"name":"Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang Regency 45363, West Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3081-9063","authenticated-orcid":false,"given":"Pringgo Kusuma Dwi Noor Yadi","family":"Putra","sequence":"additional","affiliation":[{"name":"Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang Regency 45363, West Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4613-7104","authenticated-orcid":false,"given":"Mochammad Ikhsan Cahya","family":"Utama","sequence":"additional","affiliation":[{"name":"Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang Regency 45363, West Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Elva Yenizar","family":"Anggraeni","sequence":"additional","affiliation":[{"name":"Department of Fisheries, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang Regency 45363, West Java, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.13057\/biodiv\/d230340","article-title":"Reproductive Aspects of Javaen Barb Fish, Systomus orphoides in the Initial Domestication Program","volume":"23","author":"Susatyo","year":"2022","journal-title":"Biodiversitas"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hilia, S., Dwiranti, A., Bowolaksono, A., Lestari, R., and Kritanto, A. 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