{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T10:25:36Z","timestamp":1789122336438,"version":"build-2803163510"},"reference-count":60,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018994","name":"National Science, Research and Innovation Fund","doi-asserted-by":"publisher","award":["203989"],"award-info":[{"award-number":["203989"]}],"id":[{"id":"10.13039\/501100018994","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Oxidative stress and chronic inflammation are major contributors to skin photoaging, highlighting the need for natural antioxidants with multifunctional bioactivities. This study developed an ultrasound-assisted extraction (UAE) strategy to recover an astaxanthin-rich extract from the underutilized freshwater macroalga Chara corallina and evaluated its antioxidant, anti-inflammatory, and anti-photoaging properties. Different solvent systems were evaluated for extraction efficiency, and astaxanthin-equivalent recovery was quantified by high-performance liquid chromatography with photodiode array detection (HPLC\u2013PDA). Antioxidant activity was evaluated using the DPPH radical scavenging assay, whereas biological activities were assessed in human dermal fibroblasts and RAW264.7 macrophages using cell viability assays, quantitative real-time PCR, and extracellular matrix-related enzyme inhibition assays. The selected solvent system (48% ethanol in ethyl acetate) provided the highest astaxanthin-equivalent recovery (0.2598 \u00b1 0.0086% w\/w). The selected CCE exhibited DPPH radical-scavenging activity, modulated antioxidant- and inflammation-associated gene expression, increased COL1A2 mRNA expression, and inhibited collagenase, elastase, and hyaluronidase within the evaluated concentration range. HPLC\u2013PDA analysis revealed a chromatographic component with retention-time and UV\u2013visible spectral characteristics corresponding to those of an authentic astaxanthin reference standard; however, comprehensive structural and phytochemical characterization was not performed. Because CCE is a chemically complex extract, the observed biological responses cannot be attributed exclusively to astaxanthin. Overall, these findings provide an initial basis for further investigation of C. corallina as an underexplored freshwater source of carotenoid-containing bioactive extracts rather than establishing it as a commercially competitive source of natural astaxanthin.<\/jats:p>","DOI":"10.3390\/antiox15091159","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T09:09:40Z","timestamp":1789117780000},"page":"1159","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ultrasound-Assisted Recovery and Biological Evaluation of an Astaxanthin-Containing Extract from Chara corallina"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2555-6919","authenticated-orcid":false,"given":"Natthrit","family":"Roekngam","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat 80240, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Wanninee","family":"Chankaew","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat 80110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3233-0111","authenticated-orcid":false,"given":"Suwichak","family":"Chaisit","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Pharmaceutical Chemistry, School of Engineering and Technology, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonsawan","family":"Kongpuckdee","sequence":"additional","affiliation":[{"name":"Faculty of Health and Sports Science, Thaksin University, Pa Phayom, Phatthalung 93210, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunisa","family":"Khongthong","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat 80240, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Davinelli, S., Nielsen, M.E., and Scapagnini, G. (2018). Astaxanthin in Skin Health, Repair, and Disease: A Comprehensive Review. Nutrients, 10.","DOI":"10.3390\/nu10040522"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Catanzaro, E., Bishayee, A., and Fimognari, C. (2020). On a Beam of Light: Photoprotective Activities of the Marine Carotenoids Astaxanthin and Fucoxanthin in Suppression of Inflammation and Cancer. Mar. Drugs, 18.","DOI":"10.3390\/md18110544"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Hoang, H.T., Moon, J.-Y., and Lee, Y.-C. (2021). Natural Antioxidants from Plant Extracts in Skincare Cosmetics: Recent Applications, Challenges and Perspectives. Cosmetics, 8.","DOI":"10.3390\/cosmetics8040106"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"128","DOI":"10.3390\/md12010128","article-title":"Astaxanthin: Sources, Extraction, Stability, Biological Activities and Its Commercial Applications\u2014A Review","volume":"12","author":"Ambati","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"43","DOI":"10.18388\/abp.2012_2168","article-title":"Cosmetic Benefits of Astaxanthin on Human Subjects","volume":"59","author":"Tominaga","year":"2012","journal-title":"Acta Biochim. Pol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Generali\u0107 Mekini\u0107, I., \u0160imat, V., Rathod, N.B., Hamed, I., and \u010cagalj, M. (2023). Algal Carotenoids: Chemistry, Sources, and Application. Foods, 12.","DOI":"10.3390\/foods12142768"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Nishida, Y., Berg, P.C., Shakersain, B., Hecht, K., Takikawa, A., Tao, R., Kakuta, Y., Uragami, C., Hashimoto, H., and Misawa, N. (2023). Astaxanthin: Past, Present, and Future. Mar. Drugs, 21.","DOI":"10.3390\/md21100514"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Shah, M.M.R., Liang, Y., Cheng, J.J., and Daroch, M. (2016). Astaxanthin-Producing Green Microalga Haematococcus pluvialis: From Single Cell to High Value Commercial Products. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00531"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"121606","DOI":"10.1016\/j.biortech.2019.121606","article-title":"Recent Advances in Biorefinery of Astaxanthin from Haematococcus pluvialis","volume":"288","author":"Khoo","year":"2019","journal-title":"Bioresour. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"625","DOI":"10.3390\/md9040625","article-title":"Microalgae as Sources of Carotenoids","volume":"9","author":"Guedes","year":"2011","journal-title":"Mar. Drugs"},{"key":"ref_11","first-page":"296","article-title":"Evaluation of Antioxidant Activity and Phytochemical from Kamkung (Chara corallina Klein ex C.L. Willdenow)","volume":"12","author":"Chankaew","year":"2020","journal-title":"Princess Naradhiwas Univ. J."},{"key":"ref_12","first-page":"116","article-title":"In Vitro Antioxidant and Anti-Tyrosinase Activities along with Phytochemical Constituents of Brittle Wort (Chara corallina) Extracts","volume":"47","author":"Khongsai","year":"2023","journal-title":"J. Fish. Environ."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S0304-3770(99)00095-9","article-title":"Pigment Composition of Freshwater Charophyceae","volume":"67","author":"Schagerl","year":"2000","journal-title":"Aquat. Bot."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.algal.2016.09.008","article-title":"Supercritical Fluid Extraction as a Tool to Valorize Underexploited Freshwater Green Algae","volume":"19","author":"Fabrowska","year":"2016","journal-title":"Algal Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Panda, D., and Manickam, S. (2019). Cavitation Technology\u2014The Future of Greener Extraction Method: A Review on the Extraction of Natural Products and Process Intensification Mechanism and Perspectives. Appl. Sci., 9.","DOI":"10.3390\/app9040766"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"105325","DOI":"10.1016\/j.ultsonch.2020.105325","article-title":"Ultrasound Assisted Extraction (UAE) of Bioactive Compounds from Fruit and Vegetable Processing By-Products: A Review","volume":"70","author":"Kumar","year":"2021","journal-title":"Ultrason. Sonochem."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Carreira-Casais, A., Otero, P., Garcia-Perez, P., Garcia-Oliveira, P., Pereira, A.G., Carpena, M., Soria-Lopez, A., Simal-Gandara, J., and Prieto, M.A. (2021). Benefits and Drawbacks of Ultrasound-Assisted Extraction for the Recovery of Bioactive Compounds from Marine Algae. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18179153"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Britton, G., Liaaen-Jensen, S., and Pfander, H. (2004). Carotenoids Handbook, Birkh\u00e4user Verlag.","DOI":"10.1007\/978-3-0348-7836-4"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"106932","DOI":"10.1016\/j.jfca.2024.106932","article-title":"Development of a Simple and Economical Analytical Method for Investigating the Distribution of Astaxanthin E\/Z-Isomers in Foods and Cosmetics","volume":"137","author":"Ghosh","year":"2025","journal-title":"J. Food Compos. Anal."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0023-6438(95)80008-5","article-title":"Use of a Free Radical Method to Evaluate Antioxidant Activity","volume":"28","author":"Cuvelier","year":"1995","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_21","unstructured":"Freshney, R.I. (2015). Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, John Wiley & Sons."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.7150\/ijms.46265","article-title":"Global Gene Expression of Cultured Human Dermal Fibroblasts: Focus on Cell Cycle and Proliferation Status in Improving the Condition of Face Skin","volume":"18","author":"Wilk","year":"2021","journal-title":"Int. J. Med. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Lahmar, A., Abdelaziz, B., Gouader, N., Salek, A., Waer, I., and Ghedira, L.C. (2026). Astaxanthin as a Natural Photoprotective Agent: In Vitro and In Silico Approach to Explore a Multi-Targeted Compound. Sci. Pharm., 94.","DOI":"10.3390\/scipharm94010008"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1111\/php.13034","article-title":"Intracellular Signaling Mechanisms Involved in the Biological Effects of the Xanthophyll Carotenoid Astaxanthin to Prevent the Photo-Aging of the Skin in a Reactive Oxygen Species Depletion-Independent Manner: The Key Role of Mitogen and Stress-Activated Protein Kinase 1","volume":"95","author":"Imokawa","year":"2019","journal-title":"Photochem. Photobiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1038\/nri3073","article-title":"Protective and Pathogenic Functions of Macrophage Subsets","volume":"11","author":"Murray","year":"2011","journal-title":"Nat. Rev. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"116362","DOI":"10.1016\/j.jep.2023.116362","article-title":"Anti-Inflammatory Effects of Peucedanum japonicum Thunberg Leaves Extract in Lipopolysaccharide-Stimulated RAW264.7 Cells","volume":"309","author":"Park","year":"2023","journal-title":"J. Ethnopharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1159\/000371708","article-title":"Role of Age-Associated Alterations of the Dermal Extracellular Matrix Microenvironment in Human Skin Aging: A Mini-Review","volume":"61","author":"Quan","year":"2015","journal-title":"Gerontology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"a015370","DOI":"10.1101\/cshperspect.a015370","article-title":"Natural and Sun-Induced Aging of Human Skin","volume":"5","author":"Fisher","year":"2015","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1093\/biomet\/52.3-4.591","article-title":"An Analysis of Variance Test for Normality (Complete Samples)","volume":"52","author":"Shapiro","year":"1965","journal-title":"Biometrika"},{"key":"ref_33","unstructured":"Levene, H. (1960). Robust Tests for Equality of Variances. Contributions to Probability and Statistics, Stanford University Press."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/j.ultsonch.2016.06.035","article-title":"Ultrasound Assisted Extraction of Food and Natural Products. Mechanisms, Techniques, Combinations, Protocols and Applications. A Review","volume":"34","author":"Chemat","year":"2017","journal-title":"Ultrason. Sonochem."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Patel, M.G.C., Ajith, B.S., Shettigar, A.K., and Samuel, O.D. (2025). Studies on Oil Extraction, Biodiesel Production Methods, and Engine Performance and Emission Characteristics. Biofuel Production, Performance, and Emission Optimization: A Comprehensive Approach to Modelling and Optimization, Springer.","DOI":"10.1007\/978-3-031-84806-3_2"},{"key":"ref_36","unstructured":"Rodriguez-Amaya, D.B. (2001). A Guide to Carotenoid Analysis in Foods, ILSI Press."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s13399-025-06951-5","article-title":"Ultrasound Assisted Extraction as an Emerging Sustainable Platform towards Establishment of Seaweed Biorefinery\u2014A Detailed Overview","volume":"16","author":"Shambhulingaiah","year":"2026","journal-title":"Biomass Convers. Biorefinery"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1111\/pre.12191","article-title":"Isolation of Chlorophylls and Carotenoids from Freshwater Algae Using Different Extraction Methods","volume":"66","author":"Fabrowska","year":"2018","journal-title":"Phycol. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jbiotec.2018.07.010","article-title":"Extraction of Astaxanthin from Microalga Haematococcus pluvialis in Red Phase by Using Generally Recognized as Safe Solvents and Accelerated Extraction","volume":"283","author":"Molino","year":"2018","journal-title":"J. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"13898","DOI":"10.1021\/acssuschemeng.9b02089","article-title":"One-Pot, Simultaneous Cell Wall Disruption and Complete Extraction of Astaxanthin from Haematococcus pluvialis at Room Temperature","volume":"7","author":"Irshad","year":"2019","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Antunes Filho, S., Santana dos Santos, M., dos Santos, O.A.L., Pizzorno Backx, B., Soran, M.-L., Opri\u015f, O., Lung, I., Stegarescu, A., and Boudina, M. (2023). Biosynthesis of Nanoparticles Using Plant Extracts and Essential Oils. Molecules, 28.","DOI":"10.3390\/molecules28073060"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"137437","DOI":"10.1016\/j.foodchem.2023.137437","article-title":"Ultrasound-Assisted Extraction of Carotenoids from Phytoene-Accumulating Chlorella sorokiniana Microalgae: Effect of Milling and Performance of the Green Biosolvents 2-Methyltetrahydrofuran and Ethyl Lactate","volume":"434","year":"2024","journal-title":"Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Horta, A., Duarte, A.M., Barroso, S., Pinto, F.R., Mendes, S., Lima, V., Saraiva, J.A., and Gil, M.M. (2024). Extraction of Antioxidants from Brown Macroalgae Fucus spiralis. Molecules, 29.","DOI":"10.3390\/molecules29102271"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Oslan, S.N.H., Shoparwe, N.F., Yusoff, A.H., Rahim, A.A., Chang, C.S., Tan, J.S., Oslan, S.N., Arumugam, K., Ariff, A.B., and Sulaiman, A.Z. (2021). A Review on Haematococcus pluvialis Bioprocess Optimization of Green and Red Stage Culture Conditions for the Production of Natural Astaxanthin. Biomolecules, 11.","DOI":"10.3390\/biom11020256"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Ganjeh, A.M., da Silva, L.P., Pinto, C.A., Martins, R., Marques, A.P., and Saraiva, J.A. (2026). Recent Advances of Emergent Extraction Technologies to Enhance Bioactive Compounds Extraction of Microalgae\u2014An Overview towards Application in Cosmetics. Int. J. Cosmet. Sci., in press.","DOI":"10.1111\/ics.70078"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"125736","DOI":"10.1016\/j.biortech.2021.125736","article-title":"Using Green Alga Haematococcus pluvialis for Astaxanthin and Lipid Co-Production: Advances and Outlook","volume":"340","author":"Ren","year":"2021","journal-title":"Bioresour. Technol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Bj\u00f8rklund, G., Gasmi, A., Lenchyk, L., Shanaida, M., Zafar, S., Mujawdiya, P.K., Lysiuk, R., Antonyak, H., Noor, S., and Akram, M. (2022). The Role of Astaxanthin as a Nutraceutical in Health and Age-Related Conditions. Molecules, 27.","DOI":"10.3390\/molecules27217167"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.2147\/CCID.S461753","article-title":"Ex Vivo Evaluation of a Liposome-Mediated Antioxidant Delivery System on Markers of Skin Photoaging and Skin Penetration","volume":"17","author":"Min","year":"2024","journal-title":"Clin. Cosmet. Investig. Dermatol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3289","DOI":"10.1016\/j.bbagen.2012.11.020","article-title":"Glutathione Peroxidases","volume":"1830","author":"Maiorino","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_50","first-page":"191","article-title":"The Potential of Astaxanthin as a Natural Compound for Decelerating Skin Aging: An Update Review","volume":"6","author":"Sariharyanti","year":"2024","journal-title":"Aging Pathobiol. Ther."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Ma, Y., Li, C., Su, W., Sun, Z., Gao, S., Xie, W., Zhang, B., and Sui, L. (2025). Carotenoids in Skin Photoaging: Unveiling Protective Effects, Molecular Insights, and Safety and Bioavailability Frontiers. Antioxidants, 14.","DOI":"10.3390\/antiox14050577"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"a001651","DOI":"10.1101\/cshperspect.a001651","article-title":"The Nuclear Factor NF-\u03baB Pathway in Inflammation","volume":"1","author":"Lawrence","year":"2009","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1038\/nature07201","article-title":"Origin and Physiological Roles of Inflammation","volume":"454","author":"Medzhitov","year":"2008","journal-title":"Nature"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s12079-023-00743-0","article-title":"Skin Aging from the Perspective of Dermal Fibroblasts: The Interplay between the Adaptation to the Extracellular Matrix Microenvironment and Cell Autonomous Processes","volume":"17","author":"Fisher","year":"2023","journal-title":"J. Cell Commun. Signal."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1001\/archderm.138.11.1462","article-title":"Mechanisms of Photoaging and Chronological Skin Aging","volume":"138","author":"Fisher","year":"2002","journal-title":"Arch. Dermatol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1146\/annurev.biochem.77.032207.120833","article-title":"Collagen Structure and Stability","volume":"78","author":"Shoulders","year":"2009","journal-title":"Annu. Rev. Biochem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s00403-007-0798-x","article-title":"Recent Advances in Characterizing Biological Mechanisms Underlying UV-Induced Wrinkles: A Pivotal Role of Fibroblast-Derived Elastase","volume":"300","author":"Imokawa","year":"2008","journal-title":"Arch. Dermatol. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"253","DOI":"10.4161\/derm.21923","article-title":"Hyaluronic Acid: A Key Molecule in Skin Aging","volume":"4","author":"Papakonstantinou","year":"2012","journal-title":"Dermato-endocrinology"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3164\/jcbn.17-35","article-title":"Protective Effects of Astaxanthin on Skin Deterioration","volume":"61","author":"Tominaga","year":"2017","journal-title":"J. Clin. Biochem. Nutr."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e13730","DOI":"10.1111\/srt.13730","article-title":"Cosmeceuticals in Photoaging: A Review","volume":"30","author":"Chan","year":"2024","journal-title":"Skin Res. Technol."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/9\/1159\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T09:23:15Z","timestamp":1789118595000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/15\/9\/1159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":60,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["antiox15091159"],"URL":"https:\/\/doi.org\/10.3390\/antiox15091159","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]}}}