{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T09:06:32Z","timestamp":1789981592069,"version":"4.0.1"},"reference-count":119,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Portuguese national funds","doi-asserted-by":"publisher","award":["UID\/50006\/2025"],"award-info":[{"award-number":["UID\/50006\/2025"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese national funds","doi-asserted-by":"publisher","award":["2024.06368.BDANA"],"award-info":[{"award-number":["2024.06368.BDANA"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese national funds","doi-asserted-by":"publisher","award":["CEECINST\/00007\/2021"],"award-info":[{"award-number":["CEECINST\/00007\/2021"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Portuguese national funds","doi-asserted-by":"publisher","award":["UID 05748"],"award-info":[{"award-number":["UID 05748"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs) that make farmed fish valuable to human health, given their bioactivity and highly unsaturated structure that also renders them exceptionally prone to oxidation during feed manufacture and storage, within the fish, and along the supply chain to the consumer. Preserving the oxidative stability, and hence the biological value, of these fatty acids is therefore a central challenge for sustainable aquaculture, made more acute by the replacement of omega-3-rich, oxidatively buffered fish oil with terrestrial oils that lower LC-PUFA content and shift the pro-\/antioxidant balance of aquafeeds. Adopting an integrative feed-to-fish-to-consumer perspective, this review examines the strategies proposed to retain and protect EPA and DHA in farmed fish, distinguishing the physiological requirements of fish from the nutritional targets relevant to humans. We first appraise, comparatively, fish oil replacement, finishing diets and nutritional programming, showing that recovery of EPA and DHA is species-, tissue- and context-dependent and seldom complete, with EPA, a key eicosanoid precursor, preferentially catabolised. Through a focused, reproducible literature search, we then evaluate antioxidant-oriented nanoencapsulation as a means of shielding marine LC-PUFAs from oxidation and improving their stability and bioavailability. Although nanoencapsulation consistently enhances oxidative stability and, in the few in vivo studies available, fish performance, the evidence is constrained by heterogeneous and frequently incomplete physicochemical characterisation, scarce storage stability and in vivo data, and largely unaddressed safety, environmental-fate, regulatory, scalability and consumer-acceptance concerns. We conclude that protecting omega-3 oxidative stability will require combining well-characterised delivery systems with species-appropriate nutrition, and we set out the standardised characterisation, required in vivo validation, and integrated safety and life-cycle assessment needed to realise antioxidant-protective, nanotechnology-enabled aquafeeds.<\/jats:p>","DOI":"10.3390\/antiox15091184","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:03:47Z","timestamp":1789977827000},"page":"1184","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Oxidative Stability and Nanoencapsulation of Marine Omega-3 LC-PUFAs for Sustainable Aquaculture and Human Health"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5122-6243","authenticated-orcid":false,"given":"Ana Lu\u00edsa","family":"Rebelo","sequence":"first","affiliation":[{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal"},{"name":"LAQV, REQUIMTE, Pra\u00e7a Coronel Pacheco n\u00ba15, 6\u00ba Andar, 4050-453 Porto, Portugal"},{"name":"Sense Test Lda., Rua Zeferino Costa 341, 4400-345 Vila Nova de Gaia, Portugal"},{"name":"CIIMAR\/CIMAR LA, Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental, Universidade do Porto, Av. General Norton de Matos S\/N, 4450-208 Matosinhos, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds F.","family":"Bai\u00e3o","sequence":"additional","affiliation":[{"name":"Sense Test Lda., Rua Zeferino Costa 341, 4400-345 Vila Nova de Gaia, Portugal"},{"name":"GreenUPorto\u2014Sustainable Agrifood Production Research Centre\/Inov4Agro, DGAOT, Faculdade de Ci\u00eancias, Universidade do Porto, Campus de Vair\u00e3o, Rua da Agr\u00e1ria 747, 4485-646 Vairao, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal"},{"name":"CIIMAR\/CIMAR LA, Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental, Universidade do Porto, Av. General Norton de Matos S\/N, 4450-208 Matosinhos, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8777-5877","authenticated-orcid":false,"given":"Sofia A. Costa","family":"Lima","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal"},{"name":"LAQV, REQUIMTE, Pra\u00e7a Coronel Pacheco n\u00ba15, 6\u00ba Andar, 4050-453 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1038\/s41586-021-03308-6","article-title":"A 20-year retrospective review of global aquaculture","volume":"591","author":"Naylor","year":"2021","journal-title":"Nature"},{"key":"ref_2","unstructured":"FAO (2024). 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