{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T21:57:04Z","timestamp":1789077424316,"version":"build-2803163510"},"reference-count":48,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2018,6,22]],"date-time":"2018-06-22T00:00:00Z","timestamp":1529625600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The present study aims to examine the protective effect of Justicia tranquebariesis on thioacetamide (TAA)-induced oxidative stress and hepatic fibrosis. Male Wister albino rats (150\u2013200 g) were divided into five groups. Group 1 was normal control. Group 2 was J. tranquebariensis (400 mg\/kg bw\/p.o.)-treated control. Group 3 was TAA (100 mg\/kg bw\/s.c.)-treated control. Groups 4 and 5 were orally administered with the leaf extract of J. tranquebariensis (400 mg\/kg bw) and silymarin (50 mg\/kg bw) daily for 10 days with a subsequent administration of a single dose of TAA (100 mg\/kg\/s.c.). Blood and livers were collected and assayed for various antioxidant enzymes (SOD, CAT, GPx, GST, GSH, and GR). Treatment with J. tranquebariensis significantly reduced liver TBARS and enhanced the activities of antioxidant enzymes in TAA-induced fibrosis rats. Concurrently, pretreatment with J. tranquebariensis significantly reduced the elevated liver markers (AST, ALT, ALP, GGT, and TB) in the blood. In addition, J. tranquebariensis- and silymarin- administered rats demonstrated the restoration of normal liver histology and reduction in fibronectin and collagen deposition. Based on these findings, J. tranquebariensis has potent liver protective functions and can alleviate thioacetamide-induced oxidative stress, hepatic fibrosis and possible engross mechanisms connected to antioxidant potential.<\/jats:p>","DOI":"10.3390\/antiox7070078","type":"journal-article","created":{"date-parts":[[2018,6,22]],"date-time":"2018-06-22T10:56:28Z","timestamp":1529664988000},"page":"78","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Protective Effect of Aqueous Extract from the Leaves of Justicia tranquebariesis against Thioacetamide-Induced Oxidative Stress and Hepatic Fibrosis in Rats"],"prefix":"10.3390","volume":"7","author":[{"given":"Kumeshini","family":"Sukalingam","sequence":"first","affiliation":[{"name":"Faculty of Medicine, International Medical School, Management and Science University, Shah Alam 40100, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3198-6066","authenticated-orcid":false,"given":"Kumar","family":"Ganesan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, International Medical School, Management and Science University, Shah Alam 40100, Malaysia"},{"name":"Food Science and Technology Program, Beijing Normal University\u2013Hong Kong Baptist University United International College, Zhuhai 519087, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0739-3735","authenticated-orcid":false,"given":"Baojun","family":"Xu","sequence":"additional","affiliation":[{"name":"Food Science and Technology Program, Beijing Normal University\u2013Hong Kong Baptist University United International College, Zhuhai 519087, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,6,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.biopha.2017.01.165","article-title":"Hepatoprotective activity of Butea monosperma bark against thioacetamide-induced liver injury in rats","volume":"89","author":"Kaur","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.lfs.2017.08.019","article-title":"Naringin attenuates thioacetamide-induced liver fibrosis in rats through modulation of the PI3K\/Akt pathway","volume":"187","author":"Kenawy","year":"2017","journal-title":"Life Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/j.cld.2006.08.022","article-title":"Reversal of fibrosis: No longer a pipe dream?","volume":"10","author":"Fallowfield","year":"2006","journal-title":"Clin. Liver Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"72","DOI":"10.4103\/1319-3767.45072","article-title":"Reversal of liver fibrosis","volume":"15","author":"Ismail","year":"2009","journal-title":"Saudi J. Gastroenterol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Schuppan, D., Ashfaq-Khan, M., Yang, A.T., and Kim, Y.O. (2018). Liver fibrosis: Direct antifibrotic agents and targeted therapies. Matrix Biol.","DOI":"10.1016\/j.matbio.2018.04.006"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"11033","DOI":"10.3748\/wjg.v20.i32.11033","article-title":"Chronic hepatitis C and liver fibrosis","volume":"20","author":"Sebastiani","year":"2014","journal-title":"World J. Gastroenterol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1016\/j.sjbs.2016.01.029","article-title":"Chemoprotective effect of omega-3 fatty acids on thioacetamide-induced hepatic fibrosis in male rats","volume":"24","year":"2017","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Ganesan, K., Sukalingam, K., and Xu, B. (2017). Solanum trilobatum L. ameliorate thioacetamide-induced oxidative stress and hepatic damage in albino rats. Antioxidants, 6.","DOI":"10.3390\/antiox6030068"},{"key":"ref_9","first-page":"1","article-title":"Role of zinc oxide nanoparticles in alleviating hepatic fibrosis and nephrotoxicity induced by thioacetamide in rats","volume":"16","author":"Bashandy","year":"2017","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.suronc.2017.04.006","article-title":"Efficacy and safety of combination therapy of chemoembolization and radiofrequency ablation with different time intervals for hepatocellular carcinoma patients","volume":"26","author":"Feng","year":"2017","journal-title":"Surg. Oncol."},{"key":"ref_11","first-page":"507","article-title":"Hepatoprotective evaluation of aqueous-ethanolic extract of Capparis decidua (Stems) in paracetamol-induced hepatotoxicity in experimental rabbits","volume":"30","author":"Rehman","year":"2017","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_12","unstructured":"Prajapathi, N.D., Purohit, S.S., Sharma, A.K., and Kumar, T. (2003). A Hand Book of Medicinal Plants: A Complete Source of Book, Agrobios Publisher. [1st ed.]."},{"key":"ref_13","first-page":"368","article-title":"Ethnic information on treatments for snake bites in Kadapa district of Andhra Pradesh","volume":"12","author":"Sekhar","year":"2011","journal-title":"Life Sci. Leaflet"},{"key":"ref_14","first-page":"14","article-title":"Studies on anti-inflammatory activity of leaf extract of Justicia tranquebariensis L.","volume":"17","author":"Akilandeswari","year":"2001","journal-title":"Indian J. Nat. Prod."},{"key":"ref_15","first-page":"49","article-title":"Antipyretic activity of extract of leaves of Justicia tranquebariensis (Linn) in albino mice","volume":"4","author":"Begum","year":"2009","journal-title":"Pharmacist"},{"key":"ref_16","first-page":"558","article-title":"Lignans from Justicia tranquebariensis Linn","volume":"28","author":"Raju","year":"1989","journal-title":"Indian J. Chem."},{"key":"ref_17","first-page":"116","article-title":"Preliminary phytochemical screening, free radical scavenging and antimicrobial activities of Justicia tranquebariensis L.","volume":"1","author":"Balamurugan","year":"2008","journal-title":"Res. J. Pharm. Technol."},{"key":"ref_18","first-page":"989","article-title":"Protective and curative effects of Justicia tranquebariensis (Linn) leaves in acetaminophen-induced hepatotoxicity","volume":"2","author":"Begum","year":"2011","journal-title":"Int. J. Pharm. Biol. Arch."},{"key":"ref_19","first-page":"82","article-title":"Hepatoprotective activity of a phytotherapeutic formula on thioacetamide-induced liver fibrosis model","volume":"82","author":"Kantah","year":"2011","journal-title":"Acta Biomed."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.jep.2003.12.009","article-title":"Hepatoprotective activity of Trianthema portulacastrum L. against paracetamol and thioacetamide intoxication in albino rats","volume":"92","author":"Kumar","year":"2004","journal-title":"J. Ethnopharmacol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0003-2697(79)90738-3","article-title":"Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction","volume":"95","author":"Ohkawa","year":"1979","journal-title":"Anal. Biochem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1111\/j.1432-1033.1974.tb03714.x","article-title":"Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase","volume":"47","author":"Marklund","year":"1974","journal-title":"Eur. J. Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/0003-2697(72)90132-7","article-title":"Colorimetric assay of catalase","volume":"47","author":"Sinha","year":"1972","journal-title":"Anal. Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1016\/0006-2952(87)90087-6","article-title":"Formation and reduction of glutathione-protein mixed disulfides during oxidative stress: A study with isolated hepatocytes and menadione (2-methyl-1,4-naphthoquinone)","volume":"36","author":"Bellomo","year":"1987","journal-title":"Biochem. Pharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1126\/science.179.4073.588","article-title":"Selenium: Biochemical role as a component of glutathione peroxidase","volume":"179","author":"Rotruck","year":"1973","journal-title":"Science"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"7130","DOI":"10.1016\/S0021-9258(19)42083-8","article-title":"Glutathione S-transferases: The first enzymatic step in mercapturic acid formation","volume":"249","author":"Habig","year":"1974","journal-title":"J. Biol. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/0304-4165(79)90289-7","article-title":"Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver","volume":"582","author":"Moron","year":"1979","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/BF02912891","article-title":"Biochemical activity of selenium and glutathione on country made liquor (CML) induced hepatic damage in rats","volume":"22","author":"Kumar","year":"2007","journal-title":"Indian J. Clin. Biochem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.jcma.2016.10.014","article-title":"Hepatoprotective effects of naturally fermented noni juice against thioacetamide-induced liver fibrosis in rats","volume":"80","author":"Lin","year":"2017","journal-title":"J. Chin. Med. Assoc."},{"key":"ref_31","first-page":"321","article-title":"Effects of Arrack on liver protein of Mus musculus","volume":"18","author":"Kumar","year":"2006","journal-title":"J. Ecobiol."},{"key":"ref_32","first-page":"951","article-title":"Evidences of hepatoprotective and antioxidant effect of Citrullus colocynthis fruits in paracetamol-induced hepatotoxicity","volume":"28","author":"Vakiloddin","year":"2015","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1179\/1351000214Y.0000000115","article-title":"Experimental liver protection of n-butanolic extract of Astragalus monspessulanus L. on carbon tetrachloride model of toxicity in rat","volume":"20","author":"Simeonova","year":"2015","journal-title":"Redox Rep."},{"key":"ref_34","first-page":"351","article-title":"Antihepatotoxic effect of \u03b2-carotene on paracetamol induced hepatic damage in rats","volume":"43","author":"Kumar","year":"2005","journal-title":"Indian J. Exp. Biol."},{"key":"ref_35","first-page":"315","article-title":"Ameliorate the effect of Solanum trilobatum L. on hepatic enzymes in experimental diabetes","volume":"7","author":"Kumar","year":"2011","journal-title":"Nat. Prod. Indian J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1590\/s0004-2803.201700000-03","article-title":"Action of vitamin E on experimental severe acute liver failure","volume":"54","author":"Miguel","year":"2017","journal-title":"Arq. Gastroenterol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.physbeh.2017.07.010","article-title":"Silymarin ameliorates experimentally induced depressive-like behavior in rats: Involvement of hippocampal BDNF signaling, inflammatory cytokines, and oxidative stress response","volume":"179","author":"Thakare","year":"2017","journal-title":"Physiol. Behav."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Vahabzadeh, M., Amiri, N., and Karimi, G. (2018). Effects of Silymarin on the Metabolic Syndrome; a Review. J. Sci. Food Agric.","DOI":"10.1002\/jsfa.9115"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Mazhari, S., Razi, M., and Sadrkhanlou, R. (2018). Silymarin and celecoxib ameliorate experimental varicocele-induced pathogenesis: Evidences for oxidative stress and inflammation inhibition. Int. Urol. Nephrol.","DOI":"10.1007\/s11255-018-1862-5"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/j.biopha.2018.03.140","article-title":"Antioxidant effects and mechanism of silymarin in oxidative stress-induced cardiovascular diseases","volume":"102","author":"Taleb","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.biopha.2018.02.075","article-title":"Silymarin ameliorates expression of urotensin II (U-II) and its receptor (UTR) and attenuates toxic oxidative stress in the heart of rats with type 2 diabetes","volume":"101","author":"Rahimi","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.biopha.2018.02.064","article-title":"Silymarin and caffeine combination ameliorates experimentally-induced hepatic fibrosis through down-regulation of LPAR1 expression","volume":"101","author":"Eraky","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.apjtm.2017.03.023","article-title":"The protection of Thymus vulgaris leaves alcoholic extract against hepatotoxicity of alcohol in rats","volume":"10","author":"Shaffie","year":"2017","journal-title":"Asian Pac. J. Trop. Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"51","DOI":"10.2165\/00044011-200222010-00007","article-title":"Pharmacology of silymarin","volume":"22","author":"Fraschini","year":"2002","journal-title":"Clin. Drug Investig."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.2174\/092986706776360950","article-title":"Silymarin as a new hepatoprotective agent in experimental cholestasis: New possibilities for an ancient medication","volume":"13","author":"Crocenzi","year":"2006","journal-title":"Curr. Med. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"315","DOI":"10.2174\/092986707779941159","article-title":"Silybin and silymarin-New and emerging applications in medicine","volume":"14","author":"Kren","year":"2007","journal-title":"Curr. Med. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"103","DOI":"10.3892\/mmr.2016.5969","article-title":"Anti-proliferative activity of epigallocatechin-3-gallate and silibinin on soft tissue sarcoma cells","volume":"15","author":"Harati","year":"2017","journal-title":"Mol. Med. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"157456","DOI":"10.1155\/2013\/157456","article-title":"Mechanism of hepatoprotective effect of Boesenbergia rotunda in thioacetamide-induced liver damage in rats","volume":"2013","author":"Salama","year":"2013","journal-title":"Evid. Based Complement. Altern. Med."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/7\/7\/78\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:09:43Z","timestamp":1760195383000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/7\/7\/78"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,22]]},"references-count":48,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2018,7]]}},"alternative-id":["antiox7070078"],"URL":"https:\/\/doi.org\/10.3390\/antiox7070078","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,22]]}}}