{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T23:21:25Z","timestamp":1785194485022,"version":"3.55.0"},"reference-count":49,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T00:00:00Z","timestamp":1580083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidad de Las Am\u00e9ricas","award":["VET.JMA.17.05"],"award-info":[{"award-number":["VET.JMA.17.05"]}]},{"DOI":"10.13039\/501100010198","name":"MINECO","doi-asserted-by":"publisher","award":["AGL2015-64522-C2"],"award-info":[{"award-number":["AGL2015-64522-C2"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FEDER-Interreg Espa\u00f1a-Portugal Programme","award":["0377_IBERPHENOL_6_E"],"award-info":[{"award-number":["0377_IBERPHENOL_6_E"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Pechiche fruits (Vitex cymosa Berteo ex Speng) from Ecuador were studied to determine their phenolic acid profile, nutrient minerals and capacity to protect primary human dermal fibroblasts (HDFa) against oxidative-induced damage. Up to five phenolic acids were identified, with homovanillic acid as the main one. Vitamin C, \u03b2-carotene and lutein were also determined. Phosphorus and potassium were the main macrominerals, while iron was the principal micromineral. HDFa were preincubated with a crude pechiche extract (PCext) and then subjected to oxidative stress. The activity of five antioxidant enzymes, intracellular reactive oxygen species (ROS) and ATP levels and lipid peroxidation and protein oxidation were used as markers of oxidative damage. Preincubation with PCext for 24 h allowed for the significant reduction of intracellular ROS levels, improved the intracellular ATP levels and protected lipids and proteins against oxidative damage (p &lt; 0.05). Additionally, preincubation with PCext was also able to significantly (p &lt; 0.05) improve the activity of the antioxidant enzymes catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and glutathione transferase, compared to the stressed group without pretreatment. The results obtained in this study suggest the potential of pechiche as a source of bioactive compounds, as well as its beneficial effect against oxidative stress.<\/jats:p>","DOI":"10.3390\/antiox9020109","type":"journal-article","created":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T11:41:57Z","timestamp":1580125317000},"page":"109","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Pechiche (Vitex cymosa Berteo ex Speng), a Nontraditional Fruit from Ecuador, is a Dietary Source of Phenolic Acids and Nutrient Minerals, in Addition to Efficiently Counteracting the Oxidative-Induced Damage in Human Dermal Fibroblasts"],"prefix":"10.3390","volume":"9","author":[{"given":"Mabel","family":"Guevara","sequence":"first","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Biotecnolog\u00eda Aplicada a Biomedicina (BIOMED). Universidad de Las Am\u00e9ricas, Quito 170125, Ecuador"},{"name":"Grupo de Investigaci\u00f3n en Polifenoles, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1587-2234","authenticated-orcid":false,"given":"Luis A.","family":"Vald\u00e9s-Silverio","sequence":"additional","affiliation":[{"name":"Glendale Community College, Glendale, AZ 85302, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda G.","family":"Granda-Albuja","sequence":"additional","affiliation":[{"name":"Laboratorios de Investigaci\u00f3n. Universidad de Las Am\u00e9ricas, Quito 170125, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Iturralde","sequence":"additional","affiliation":[{"name":"Laboratorios de Investigaci\u00f3n. Universidad de Las Am\u00e9ricas, Quito 170125, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Jaramillo-Vivanco","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Biotecnolog\u00eda Aplicada a Biomedicina (BIOMED). Universidad de Las Am\u00e9ricas, Quito 170125, Ecuador"},{"name":"Jard\u00edn Bot\u00e1nico de Quito, Quito 170125, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8151-9132","authenticated-orcid":false,"given":"Francesca","family":"Giampieri","sequence":"additional","affiliation":[{"name":"Nutrition and Food Science Group, Department of Analytical and Food Chemistry, CITACA, CACTI, University of Vigo, Vigo Campus, 36310 Vigo, Spain"},{"name":"Department of Clinical Sciences, Universit\u00e1 Politecnica delle Marche, 60131 Ancona, Italy"},{"name":"College of Food Science and Technology, Northwest University, Xi\u2019an 710069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6592-5299","authenticated-orcid":false,"given":"Celestino","family":"Santos-Buelga","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Polifenoles, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5477-0703","authenticated-orcid":false,"given":"Ana M.","family":"Gonz\u00e1lez-Param\u00e1s","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Polifenoles, Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7250-1782","authenticated-orcid":false,"given":"Maurizio","family":"Battino","sequence":"additional","affiliation":[{"name":"Nutrition and Food Science Group, Department of Analytical and Food Chemistry, CITACA, CACTI, University of Vigo, Vigo Campus, 36310 Vigo, Spain"},{"name":"Department of Clinical Sciences, Universit\u00e1 Politecnica delle Marche, 60131 Ancona, Italy"},{"name":"International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8509-1498","authenticated-orcid":false,"given":"Jos\u00e9 M.","family":"\u00c1lvarez-Suarez","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Biotecnolog\u00eda Aplicada a Biomedicina (BIOMED). Universidad de Las Am\u00e9ricas, Quito 170125, Ecuador"},{"name":"King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"757","DOI":"10.2147\/CIA.S158513","article-title":"Oxidative stress, aging, and diseases","volume":"13","author":"Liguori","year":"2018","journal-title":"Clin. Interv. Aging"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1093\/gerona\/glx227","article-title":"Health Benefits of the Mediterranean Diet: Metabolic and Molecular Mechanisms","volume":"73","author":"Tosti","year":"2018","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1007\/s00394-012-0380-y","article-title":"Critical review: vegetables and fruit in the prevention of chronic diseases","volume":"51","author":"Boeing","year":"2012","journal-title":"Eur. J. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"223","DOI":"10.3233\/JBR-180316","article-title":"Wild Andean blackberry (Rubus glaucus Benth) and Andean blueberry (Vaccinium floribundum Kunth) from the Highlands of Ecuador: Nutritional composition and protective effect on human dermal fibroblasts against cytotoxic oxidative damage","volume":"8","author":"Iturralde","year":"2018","journal-title":"J. Berry Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.fct.2017.01.024","article-title":"Anti-inflammatory effect of Capuli cherry against LPS-induced cytotoxic damage in RAW 264.7 macrophages","volume":"102","author":"Aller","year":"2017","journal-title":"Food Chem. Toxicol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1007\/s11130-019-00744-8","article-title":"Chemical Composition and Antioxidant Activity of the Main Fruits, Tubers and Legumes Traditionally Consumed in the Andean Regions of Ecuador as a Source of Health-Promoting Compounds","volume":"74","author":"Tejera","year":"2019","journal-title":"Plant Foods Hum. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"49","DOI":"10.3233\/JBR-140093","article-title":"Andean berries from Ecuador: A review on Botany, Agronomy, Chemistry and Health Potential","volume":"5","author":"Aller","year":"2015","journal-title":"J. Berry Res."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Guevara, M., Tejera, E., Granda-Albuja, M.G., Iturralde, G., Chisaguano-Tonato, M., Granda-Albuja, S., Jaramillo-Vivanco, T., Giampieri, F., Battino, M., and Alvarez-Suarez, J.M. (2019). Chemical Composition and Antioxidant Activity of the Main Fruits Consumed in the Western Coastal Region of Ecuador as a Source of Health-Promoting Compounds. Antioxidants, 8.","DOI":"10.3390\/antiox8090387"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1021\/jf802656r","article-title":"Phenolic Compounds in Rosaceae Fruits from Ecuador","volume":"57","author":"Vasco","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"8274","DOI":"10.1021\/jf9013586","article-title":"Chemical composition and phenolic compound profile of morti\u00f1o (vaccinium floribundum kunth)","volume":"57","author":"Vasco","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1016\/j.foodchem.2008.04.054","article-title":"Total phenolic compounds and antioxidant capacities of major fruits from Ecuador","volume":"111","author":"Vasco","year":"2008","journal-title":"Food Chem."},{"key":"ref_12","unstructured":"Aguirre, Z. (2020, January 26). Especies forestales de los bosques secos del Ecuador. Guia dendrol\u00f3gica 393 para su identificaci\u00f3n y caracterizaci\u00f3n. Proyecto Manejo Forestal Sostenible ante el Cambio; Quito, Ecuador. Available online: https:\/\/coin.fao.org\/coin-static\/cms\/media\/21\/14042335632720\/especies_forestales_bosques_secos_del_ecuador.pdf."},{"key":"ref_13","first-page":"19","article-title":"Caracterizaci\u00f3n nutricional y capacidad antioxidante del fruto del pechiche (Vitex gigantea Kunth)","volume":"27","author":"Alicia","year":"2018","journal-title":"Cienc. Tecnol. Aliment."},{"key":"ref_14","unstructured":"De la Torre, L., Muriel, M., Navarrete, H., Mac\u00eda, M.J., and Balslev, H. (2020, January 26). Enciclopedia de las Plantas \u00datiles del Ecuador; 2008. Available online: https:\/\/scholarspace.manoa.hawaii.edu\/bitstream\/10125\/47330\/de%20la%20Torre%20et%20al.%202008%20Encyclopedia%20of%20useful%20plants%20of%20Ecuador.pdf."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4603","DOI":"10.1021\/jf3011239","article-title":"Comparison of phenolic acids profile and antioxidant potential of six varieties of spelt (Triticum spelta L.)","volume":"60","author":"Dziki","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_16","unstructured":"Maines, M.D. (1998). Current Protocols in Toxicology, John Wiley & Sons, Inc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0076-6879(84)05016-3","article-title":"Catalase in vitro","volume":"105","author":"Aebi","year":"1984","journal-title":"Methods Enzymol."},{"key":"ref_18","first-page":"130","article-title":"A modified spectrophotometric assay of superoxide dismutase","volume":"21","author":"Kakkar","year":"1984","journal-title":"Indian J. Biochem. Biophys."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.fct.2016.06.003","article-title":"Strawberry consumption alleviates doxorubicin-induced toxicity by suppressing oxidative stress","volume":"94","author":"Giampieri","year":"2016","journal-title":"Food Chem. Toxicol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0003-2697(79)90738-3","article-title":"Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction","volume":"95","author":"Ohkawa","year":"1979","journal-title":"Anal. Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/0003-2697(92)90122-N","article-title":"Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low density lipoprotein","volume":"202","author":"Jiang","year":"1992","journal-title":"Anal. Biochem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/0076-6879(90)86141-H","article-title":"Determination of carbonyl content in oxidatively modified proteins","volume":"186","author":"Levine","year":"1990","journal-title":"Methods Enzymol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1089\/ars.2012.4581","article-title":"Dietary (poly)phenolics in human health: Structures, bioavailability, and evidence of protective effects against chronic diseases","volume":"18","author":"Spencer","year":"2013","journal-title":"Antioxid. Redox Signal."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/S0963-9969(00)00072-7","article-title":"Biophenolic components of olives","volume":"33","author":"Bianco","year":"2000","journal-title":"Food Res. Int."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1007\/BF02662292","article-title":"Antioxidant effect of natural phenols on olive oil","volume":"68","author":"Papadopoulos","year":"1991","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0308-8146(03)00257-7","article-title":"Determination of free and bound phenolic acids in beer","volume":"84","author":"Nardini","year":"2004","journal-title":"Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1158\/0008-5472.CAN-03-1670","article-title":"Induction of Apoptosis in Leukemic Cells by Homovanillic Acid Derivative, Capsaicin, through Oxidative Stress","volume":"64","author":"Ito","year":"2004","journal-title":"Cancer Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2017\/2367453","article-title":"Changes in Polyphenols Contents and Antioxidant Capacities of Organically and Conventionally Cultivated Tomato (Solanum lycopersicum L.) Fruits during Ripening","volume":"2017","author":"Anton","year":"2017","journal-title":"Int. J. Anal. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/S0278-6915(03)00011-5","article-title":"Isolation, structure elucidation and antioxidant potential of the major phenolic and flavonoid compounds in brined olive drupes","volume":"41","author":"Owen","year":"2003","journal-title":"Food Chem. Toxicol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1093\/aob\/mcp105","article-title":"Phylogenetics of Olea (Oleaceae) based on plastid and nuclear ribosomal DNA sequences: Tertiary climatic shifts and lineage differentiation times","volume":"104","author":"Besnard","year":"2009","journal-title":"Ann. Bot."},{"key":"ref_32","unstructured":"Lorenzi, H. (2002). Brazilian Trees: A Guide to the Identification and Cultivation of Brazilian Native Trees, Instituto Plantarum de Estudos da Flora."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.1016\/j.foodres.2011.03.051","article-title":"Bioactive compounds and antioxidant activity of fresh exotic fruits from northeastern Brazil","volume":"44","author":"Almeida","year":"2011","journal-title":"Food Res. Int."},{"key":"ref_34","unstructured":"USDA (1985). United States Standards for Grades of Extracted Honey. Agricultural Marketing Service Fruit and Vegetable Division Processed Products Branch, US Department of Agriculture."},{"key":"ref_35","unstructured":"Food and Nutrition Board Staff, Panel on Dietary Antioxidants, and Institute of Medicine Staff (2000). Dietary Reference Intakes for Vitamin C, Vitamin E, Selenium and Carotenoids: A Report of the Panel on Dietary Antioxidants and Related Compounds, Subcommitties on Upper Reference Levels of Nutrients and of the Interpretation and Use of Dietary Reference Intakes, and the Standing Committee on the Scientific Evaluation of Dietary Reference Intakes, Food and Nutrition Board, Institute of Medicine, National Academy Press."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2088","DOI":"10.1016\/j.foodres.2011.03.056","article-title":"Polyphenol composition, vitamin C content and antioxidant capacity of Mauritian citrus fruit pulps","volume":"44","author":"Ramful","year":"2011","journal-title":"Food Res. Int."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3233\/JBR-170166","article-title":"Changes in fruit maturity indices and growth pattern along the harvest season in seedless barberry under different altitude conditions","volume":"8","author":"Khayyat","year":"2018","journal-title":"J. Berry Res."},{"key":"ref_38","unstructured":"FAO, and WHO (2004). Vitamin and mineral requirements in human nutrition, Second edition, World Health Organization."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"368S","DOI":"10.3945\/an.112.003533","article-title":"Potassium and Health","volume":"4","author":"Weaver","year":"2013","journal-title":"Adv. Nutr."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1023\/A:1019998702946","article-title":"Protective role of magnesium in cardiovascular diseases: A review","volume":"238","author":"Chakraborti","year":"2002","journal-title":"Mol. Cell. Biochem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.fshw.2016.01.001","article-title":"Calcium intake, calcium homeostasis and health","volume":"5","author":"Pu","year":"2016","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0098-2997(00)00006-6","article-title":"The roles of iron in health and disease","volume":"22","author":"Lieu","year":"2001","journal-title":"Mol. Asp. Med."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Cherasse, Y., and Urade, Y. (2017). Dietary Zinc Acts as a Sleep Modulator. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18112334"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.fct.2018.01.022","article-title":"Dietary polyphenols: Structures, bioavailability and protective effects against atherosclerosis","volume":"113","author":"Santhakumar","year":"2018","journal-title":"Food Chem. Toxicol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3307","DOI":"10.1002\/jps.22578","article-title":"Analysis of 2,2\u2019-Azobis (2-Amidinopropane) Dihydrochloride Degradation and Hydrolysis in Aqueous Solutions","volume":"100","author":"Werber","year":"2011","journal-title":"J. Pharm. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Terry, L.A. (2011). Health-Promoting Properties of Fruits and Vegetables, CABI.","DOI":"10.1079\/9781845935283.0000"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3250","DOI":"10.1039\/C7FO00859G","article-title":"The protective effect of acerola (Malpighia emarginata) against oxidative damage in human dermal fibroblasts through the improvement of antioxidant enzyme activity and mitochondrial functionality","volume":"8","author":"Giampieri","year":"2017","journal-title":"Food Funct."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1007\/s11130-018-0657-2","article-title":"Guava (Psidium guajava L. cv. Red Suprema) Crude Extract Protect Human Dermal Fibroblasts against Cytotoxic Damage Mediated by Oxidative Stress","volume":"73","author":"Giampieri","year":"2018","journal-title":"Plant Foods Hum. Nutr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"7798","DOI":"10.3390\/molecules19067798","article-title":"Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality","volume":"19","author":"Giampieri","year":"2014","journal-title":"Molecules"}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/9\/2\/109\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:08:40Z","timestamp":1760364520000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/9\/2\/109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,27]]},"references-count":49,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["antiox9020109"],"URL":"https:\/\/doi.org\/10.3390\/antiox9020109","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,27]]}}}