{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T12:17:37Z","timestamp":1785932257392,"version":"3.56.0"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,8,8]],"date-time":"2020-08-08T00:00:00Z","timestamp":1596844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Penelitian Dasar Unggulan Perguruan Tinggi","award":["No 4160\/IT3.I.1\/PN\/2019"],"award-info":[{"award-number":["No 4160\/IT3.I.1\/PN\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Background: The extract of Adenostemma lavenia (L.) O. Kuntze leaves has anti-inflammatory activities and is used as a folk medicine to treat patients with hepatitis and pneumonia in China and Taiwan. The diterpenoid ent-11\u03b1-hydroxy-15-oxo-kaur-16-en-19-oic acid (11\u03b1OH-KA) is the major ingredient in the extract and has wide-spectrum biological activities, such as antitumor and antimelanogenic activities, as well as anti-inflammatory activity. However, the physical and biological properties of this compound as an antioxidant or antiaging agent have not been reported yet. Methods: In addition to in vitro assays, we monitored antioxidative and antiaging signals in Schizosaccharomyces pombe (yeast) and mouse melanoma B16F10 cells. Results: A. lavenia water and chloroform fractions showed antioxidant properties in vitro. The A. lavenia extracts and 11\u03b1OH-KA conferred resistance to H2O2 to S. pombe and B16F10 cells and extended the yeast lifespan in a concentration-dependent manner. These materials maintained the yeast mitochondrial activity, even in a high-glucose medium, and induced an antioxidant gene program, the transcriptional factor pap1+ and its downstream ctt1+. Accordingly, 11\u03b1OH-KA activated the antioxidative transcription factor NF-E2-related factor 2, NRF2, the mammalian ortholog of pap1+, in B16F10 cells, which was accompanied by enhanced hemeoxygenase expression levels. These results suggest that 11\u03b1OH-KA and A. lavenia extracts may protect yeast and mammalian cells from oxidative stress and aging. Finally, we hope that these materials could be helpful in treating COVID-19 patients, because A. lavenia extracts and NRF2 activators have been reported to alleviate the symptoms of pneumonia in model animals.<\/jats:p>","DOI":"10.3390\/antiox9080719","type":"journal-article","created":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T05:07:23Z","timestamp":1597036043000},"page":"719","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["The Antiaging Effect of Active Fractions and Ent-11\u03b1-Hydroxy-15-Oxo-Kaur-16-En-19-Oic Acid Isolated from Adenostemma lavenia (L.) O. Kuntze at the Cellular Level"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8201-7807","authenticated-orcid":false,"given":"Irmanida","family":"Batubara","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, IPB Dramaga Campus, Bogor, West Java 16680, Indonesia"},{"name":"Tropical Biopharmaca Research Center, IPB University, Taman Kencana Street No. 3, Bogor 16128, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1561-6943","authenticated-orcid":false,"given":"Rika Indri","family":"Astuti","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, IPB Dramaga Campus, Bogor, West Java 16680, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2500-1264","authenticated-orcid":false,"given":"Muhammad Eka","family":"Prastya","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University, IPB Dramaga Campus, Bogor, West Java 16680, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Auliya","family":"Ilmiawati","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, IPB Dramaga Campus, Bogor, West Java 16680, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miwa","family":"Maeda","sequence":"additional","affiliation":[{"name":"Graduate School of Natural Science and Technology, Gifu University; Gifu 501-1193, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayu","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University; Gifu 501-1193, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akie","family":"Hamamoto","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University; Gifu 501-1193, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1815-2964","authenticated-orcid":false,"given":"Hiroshi","family":"Takemori","sequence":"additional","affiliation":[{"name":"Graduate School of Natural Science and Technology, Gifu University; Gifu 501-1193, Japan"},{"name":"Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University; Gifu 501-1193, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1021\/np50002a009","article-title":"Isolation of 11-hydroxyated kauranic acids from Adenostemma lavenia","volume":"42","author":"Cheng","year":"1979","journal-title":"J. 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