{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:47:03Z","timestamp":1791895623017,"version":"4.3.4"},"reference-count":59,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T00:00:00Z","timestamp":1598400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>The aim of this study was to compare the chemical composition, as well as antioxidant, anti-inflammatory, antiacne, and cytotoxic activites of various extracts of Cephalaria gigantea and C. uralensis. It is worth underlining that we are the first to characterize the composition and evaluate the biological properties of extracts from Cephalaria gigantea and C. uralensis. Thus, the LC-DAD-MS3 analysis revealed the presence of 41 natural products in studied extracts. The 5-O-caffeoylquinic acid, isoorinetin, and swertiajaponin were the main detected compounds. Among the tested samples, ethanol extract of the aerial parts of C. uralensis (CUE) possessed the most suitable biological properties. It exhibited moderate ability to scavenge free radicals and good capacity to inhibit cyclooxygenase-1, as well as cyclooxygenase-2. Moreover, CUE possessed moderate antibacterial activity against all tested bacterial strains (S. aureus, S. epidermidis, and P. acnes), and importantly, it was non-toxic towards normal skin fibroblasts. Taking into account the value of calculated therapeutic index (&gt;10), it is worth noting that CUE can be subjected to in vivo study. Thus, CUE constitutes a very promising antiacne agent.<\/jats:p>","DOI":"10.3390\/antiox9090796","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T08:05:18Z","timestamp":1598515518000},"page":"796","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Extracts from Cephalaria Uralensis (Murray) Roem. &amp; Schult. and Cephalaria Gigantea (Ledeb.) Bobrov as Potential Agents for Treatment of Acne Vulgaris: Chemical Characterization and In Vitro Biological Evaluation"],"prefix":"10.3390","volume":"9","author":[{"given":"Ma\u0142gorzata","family":"Chrz\u0105szcz","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chod\u017aki Str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0353-123X","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Miazga-Karska","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Medical University of Lublin, 1 Chod\u017aki Str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4236-9087","authenticated-orcid":false,"given":"Katarzyna","family":"Klimek","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Medical University of Lublin, 1 Chod\u017aki Str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1876-1321","authenticated-orcid":false,"given":"Sebastian","family":"Granica","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota","family":"Tch\u00f3rzewska","sequence":"additional","affiliation":[{"name":"Department of Plant Anatomy and Cytology, Maria Curie-Sk\u0142odowska University, Akademicka 19, 20-033 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9961-4226","authenticated-orcid":false,"given":"Gra\u017cyna","family":"Ginalska","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Medical University of Lublin, 1 Chod\u017aki Str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4884-9110","authenticated-orcid":false,"given":"Katarzyna","family":"Szewczyk","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chod\u017aki Str., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,26]]},"reference":[{"key":"ref_1","unstructured":"(2020, June 14). Available online: https:\/\/www.reportlinker.com\/p05251482."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1155\/MI.2005.380","article-title":"Oxidative stress in patients with acne vulgaris","volume":"6","author":"African","year":"2005","journal-title":"Mediat. Inflamm."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.paed.2007.07.005","article-title":"The treatment of acne","volume":"17","author":"Shaw","year":"2007","journal-title":"Paediatr. Child. Health."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.clindermatol.2013.01.001","article-title":"Seborrheic dermatitis: Etiology, risk factors, and treatments: Facts and controversies","volume":"31","author":"Dessinioti","year":"2013","journal-title":"Clin. Dermatol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1111\/dth.12282","article-title":"Contact dermatitis to topical acne drugs: A review of the literature","volume":"28","author":"Foti","year":"2015","journal-title":"Dermatol. Ther."},{"key":"ref_6","first-page":"1","article-title":"Antibiotic resistance in acne treatment","volume":"17","author":"Humphrey","year":"2012","journal-title":"Skin Ther. Lett."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.ijantimicag.2016.11.021","article-title":"Revival of old antibiotics: Needs, the state of evidence and expectations","volume":"49","author":"Zayyad","year":"2017","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.drudis.2008.07.002","article-title":"Where is the hope for drug discovery? Let history tell the future","volume":"3\u20134","author":"Kong","year":"2009","journal-title":"Drug Discov. Today"},{"key":"ref_9","unstructured":"Patwardhan, B. (2005). Traditional Medicine: Modern Approach for Affordable Global Health, WHO. Available online: http:\/\/www.who.int\/intellectualproperty\/studies\/traditional_medicine\/en\/index.html."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1016\/j.drudis.2009.05.009","article-title":"Traditional medicine\u2014inspired approaches to drug discovery: Can Ayurveda show the way forward?","volume":"14","author":"Patwardhan","year":"2009","journal-title":"Drug Discov. Today"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/nrd4510","article-title":"The re-emergence of natural products for drug discovery in the genomic era","volume":"14","author":"Harvey","year":"2015","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jep.2015.06.052","article-title":"Ethnopharmacological survey of medicinal plants used in traditional medicine by the communities of Mount Hermon, Lebanon","volume":"173","author":"Baydoun","year":"2015","journal-title":"J. Ethnopharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jep.2018.08.004","article-title":"Traditional medicinal plants of A\u011fr\u0131 Province, Turkey","volume":"226","author":"Dalar","year":"2018","journal-title":"J. Ethopharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e25580","DOI":"10.5812\/jjm.25580","article-title":"Medicinal plants for the treatment of acne vulgaris: A review of recent evidences","volume":"8","author":"Nasri","year":"2015","journal-title":"Jundishapur J. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"52","DOI":"10.4103\/0973-7847.125531","article-title":"Plants used to treat skin diseases","volume":"8","author":"Tabassum","year":"2014","journal-title":"Pharmacogn. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.jep.2004.01.015","article-title":"Ethnopharmacognostic survey on the natural ingredients used in folk cosmetics, cosmeceuticals and remedies for healing skin diseases in the inland Marches, Central-Eastern Italy","volume":"91","author":"Pieroni","year":"2004","journal-title":"J. Ethnopharmacol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1055\/s-0031-1298415","article-title":"Antimicrobially active hederagenin glycosides from Cephalaria elmaliensis","volume":"78","year":"2012","journal-title":"Planta Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"497","DOI":"10.25135\/rnp.64.17.03.056","article-title":"Phytochemical and biological investigations on Cephalaria anatolica","volume":"11","author":"Sumer","year":"2017","journal-title":"Rec. Nat. Prod."},{"key":"ref_19","first-page":"141","article-title":"Chemical constituents of two endemic Cephalaria species","volume":"4","author":"Kayce","year":"2010","journal-title":"Rec. Nat. Prod."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1016\/0031-9422(94)00789-V","article-title":"A bidesmodic triterpene saponin from Cephalaria transsyvanica","volume":"38","author":"Anil","year":"1995","journal-title":"Phytochemistry"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1007\/s10600-020-02980-w","article-title":"Isolation and structural studies on saponins from three Cephalaria species from Anatolia","volume":"56","author":"Celenk","year":"2020","journal-title":"Chem. Nat. Compd."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1002\/(SICI)1099-1573(199605)10:3<274::AID-PTR822>3.0.CO;2-V","article-title":"Antimicrobial and antifungal activities of three new triterpenoid glycosides","volume":"10","author":"Akdemir","year":"1996","journal-title":"Phytoter. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.phytochem.2017.02.015","article-title":"Immunomodulatory, hemolytic properties and cytotoxic activity potent of triterpenoid saponins from Cephalaria balansae","volume":"137","author":"Top","year":"2017","journal-title":"Phytochemistry"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1007\/s10600-008-0041-9","article-title":"Iridoid glycosides from Cephalaria kotschyi roots","volume":"44","author":"Mustafayeva","year":"2008","journal-title":"Chem. Nat. Compd."},{"key":"ref_25","first-page":"667","article-title":"Alkaloids from certain Cephalaria species","volume":"5","author":"Aliev","year":"1975","journal-title":"Khim. Prir. Soedin."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1007\/s10600-009-9328-8","article-title":"Flavonoids from Cephalaria grossheimii","volume":"45","author":"Movsumov","year":"2009","journal-title":"Chem. Nat. Compd."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"345","DOI":"10.3906\/kim-1607-68","article-title":"The structure and cytotoxic activity of a new saponin: Cephoside A from Cephalaria elazigensis var","volume":"41","author":"Kayce","year":"2017","journal-title":"purpurea. Turk. J. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1021\/np900724u","article-title":"Antimicrobial triterpenoid glycosides from Cephalaria scoparia","volume":"73","year":"2010","journal-title":"J. Nat. Prod."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1021\/np960193x","article-title":"Triterpenoid saponins from Cephalaria transsylvanica","volume":"59","author":"Rose","year":"1996","journal-title":"J. Nat. Prod."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.jep.2004.03.033","article-title":"Studies on the in vitro and in vivo hypoglycemic activities of some medicinal plants used in treatment of diabetes in Jordanian traditional medicine","volume":"93","author":"Hamdan","year":"2004","journal-title":"J. Ethopharmacol."},{"key":"ref_31","unstructured":"(2011). Polish Pharmacopoeia IX, PTFarm, Polish Pharmaceutical Society."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Szewczyk, K., Bogucka-Kocka, A., Vorobets, N., Grzywa-Celi\u0144ska, A., and Granica, S. (2020). Phenolic composition of the leaves of Pyrola rotundifolia L. and their antioxidant and cytotoxic activity. Molecules, 25.","DOI":"10.3390\/molecules25071749"},{"key":"ref_33","first-page":"96","article-title":"Antioxidant properties of the extracts from different parts of broccoli in Taiwan","volume":"9","author":"Guo","year":"2001","journal-title":"J. Food Drug Anal."},{"key":"ref_34","unstructured":"Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C., and Yolke, R.H. (1995). Manual of Clinical Microbiology, Mosby Year Book. [6th ed.]."},{"key":"ref_35","unstructured":"Clinical Laboratory Standards Institute (2008). Performance Standards for Antimicrobial Susceptibility Testing. Eighteenth International Supplement, Clinical Laboratory Standards Institute. CLSI Document M7-MIC."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.phymed.2011.12.003","article-title":"Antibacterial activity of Thymus maroccanus and Thymus broussonetii essential oils against nosocomial infection\u2014bacteria and their synergistic potential with antibiotics","volume":"19","author":"Fadli","year":"2012","journal-title":"Phytomedicine"},{"key":"ref_37","first-page":"710","article-title":"In vitro activity of peptide fractions from Impatiens glandulifera against caries causing bacteria","volume":"74","author":"Szewczyk","year":"2017","journal-title":"Acta Pol. Pharm."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1007\/s00044-016-1599-6","article-title":"Synthesis, antibacterial and antiproliferative potential of some new 1-pyridinecarbonyl-4-substituted thiosemicarbazide derivatives","volume":"25","author":"Pitucha","year":"2016","journal-title":"Med. Chem. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2900","DOI":"10.1021\/jf026187q","article-title":"Hierarchical scheme for LC-MSn identification of chlorogenic acids","volume":"51","author":"Clifford","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_40","unstructured":"Clifford, M.N. (2020, June 14). Some Notes on the Chlorogenic Acids. 3. LC and LC\u2013MS. Version 3. Available online: https:\/\/www.researchgate.net\/publication\/312590947_Some_Notes_on_the_Chlorogenic_Acids_3_LC_and_LC-MS_Version_3_January_2017."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3821","DOI":"10.1021\/jf050046h","article-title":"Discriminating between the six isomers of dicaffeoylquinic acid by LC-MS(n)","volume":"53","author":"Clifford","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1007\/BF00563469","article-title":"A chemical study of Cephalaria gigantea","volume":"6","author":"Zemtsova","year":"1970","journal-title":"Chem. Nat. Compd."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1002\/pca.820","article-title":"Distinction of the C-glycosylflavone isomer pairs orientin\/isoorientin and vitexin\/isovitexin using HPLC-MS exact mass measurement and in-source CID","volume":"16","author":"Pereira","year":"2005","journal-title":"Phytochem. Anal."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.jpba.2019.05.019","article-title":"Simultaneous determination of several flavonoids and phenolic compounds in nineteen different Cephalaria species by HPLC-MS\/MS","volume":"173","author":"Goren","year":"2019","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1111\/1541-4337.12389","article-title":"A review on the dietary flavonoid tiliroside","volume":"17","author":"Grochowski","year":"2018","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1007\/s10600-011-0079-y","article-title":"Chemical constituents from the roots of Cephalaria kotschyi","volume":"47","author":"Mustafayeva","year":"2011","journal-title":"Chem. Nat. Compd."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/S0031-9422(00)00306-X","article-title":"Chemotaxonomy of Plantago. Iridoid glucosides and caffeoyl phenylethanoid glycosides","volume":"55","author":"Franzyk","year":"2000","journal-title":"Phytochemistry"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.phytol.2011.05.006","article-title":"Isolation and characterization of biologically active glycosides from endemic Cephalaria species in Anatolia","volume":"4","author":"Pekmez","year":"2011","journal-title":"Phytochem. Lett."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Gawe\u0142-B\u0119ben, K., Strz\u0119pek-Gom\u00f3\u0142ka, M., Czop, M., Sakipova, Z., G\u0142owniak, K., and Kukula-Koch, W. (2020). Achillea millefolium L. and Achillea biebersteinii Afan. hydroglycolic extracts\u2013bioactive ingredients for cosmetic use. Molecules, 25.","DOI":"10.3390\/molecules25153368"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3203","DOI":"10.1039\/C3AY41807C","article-title":"Caffeic acid: A review of its potential use in medications and cosmetic","volume":"6","author":"Magnani","year":"2014","journal-title":"Anal. Methods"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1111\/j.1468-3083.2009.03505.x","article-title":"Oxidative stress in acne vulgaris","volume":"24","author":"Sarici","year":"2010","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"156","DOI":"10.17628\/ecb.2018.7.156-161","article-title":"Total phenolic content, antioxidant capacity and antifungal activity of extracts of Carthamus tenuis and Cephalaria joppensis","volume":"7","author":"Azab","year":"2018","journal-title":"Eur. Chem. Bull."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.foodchem.2013.11.018","article-title":"Lemon grass (Cymbopogon citratus (D.C.) Stapf) polyphenols protect human umbilical vein endothelial cell (HUVECs) from oxidative damage induced by high glucose, hydrogen peroxide and oxidized low-density lipoprotein","volume":"151","author":"Campos","year":"2014","journal-title":"Food Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/S0733-8635(05)70418-6","article-title":"Acne: An overview of clinical research findings","volume":"15","author":"Thiboutot","year":"1997","journal-title":"Dermatol. Clin."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.fitote.2009.07.004","article-title":"Anti-inflammatory, cyclooxygenase (COX)-2, COX-1 inhibitory and antioxidant effects of Dysophylla stellate Benth","volume":"81","author":"Gautam","year":"2010","journal-title":"Fitoterapia"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.sajb.2007.11.009","article-title":"Cyclooxygenase inhibition and antimycobacterial effects of extracts from Sudanese medicinal plants","volume":"74","author":"Eldeen","year":"2008","journal-title":"S. Afr. J. Bot."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.indcrop.2018.06.026","article-title":"Phenolic composition, antioxidant, antibacterial and anti-inflammatory activities of leaf and stem extracts from Cryptotaenia japonica Hassk","volume":"122","author":"Lu","year":"2018","journal-title":"Ind. Crop. Prod."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1111\/j.1742-7843.2008.00301.x","article-title":"In vivo skin irritation potential of a Castanea sativa (chestnut) leaf extract, a putative natural antioxidant for topical application","volume":"103","author":"Almeida","year":"2008","journal-title":"Basic. Clin. Pharmacol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.ejpb.2004.02.013","article-title":"In vivo investigation, in mice and in man, into the irritation potential of novel amphiphilogels being studied as transdermal drug carriers","volume":"58","author":"Jibry","year":"2004","journal-title":"Eur. J. Pharm. Biopharm."}],"container-title":["Antioxidants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3921\/9\/9\/796\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:07:18Z","timestamp":1760177238000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3921\/9\/9\/796"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,26]]},"references-count":59,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,9]]}},"alternative-id":["antiox9090796"],"URL":"https:\/\/doi.org\/10.3390\/antiox9090796","relation":{},"ISSN":["2076-3921"],"issn-type":[{"value":"2076-3921","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,26]]}}}