{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:29:19Z","timestamp":1791394159492,"version":"4.3.3"},"reference-count":59,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T00:00:00Z","timestamp":1600041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Oxidative stress would play a role in the pathophysiology of sickle cell anemia (SCA). We tested the impact of common SCA genetic modifiers (alpha-thalassemia, G6PD deficiency, HbF quantitative trait loci; QTL) and pro\/antioxidant genes polymorphisms (SOD2 rs4880, XO rs207454, MPO rs2333227) on oxidative stress biomarkers (AOPP, MDA, MPO, XO, MnSOD, CAT, GPx) and clinical severity in 301 Senegalese SCA hydroxyurea-free children at steady-state (median age 9.1 years, sex ratio H\/F = 1.3). Plasma oxidative stress biomarkers were compared with those of a control group (AA). CAT activity, AOPP, and MDA levels were higher in SCA than in AA individuals while XO, GPX, and MnSOD activities were lower. The presence of alpha-thalassemia decreased MDA level and MPO activity but no effect of the HbF QTL or G6PD deficiency was observed. SCA children who experienced their first hospitalized complication before 3 years old had higher MnSOD and CAT activities than the other children while those with no hospitalized VOC in the previous 2 years presented higher GPX activity. Age of the first hospitalized complication and AOPP levels were affected by the MPO rs2333227 SNP. Our results suggest that alpha-thalassemia modulates oxidative stress in SCA, presumably because of a reduction in the MPO activity.<\/jats:p>","DOI":"10.3390\/antiox9090863","type":"journal-article","created":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T09:04:53Z","timestamp":1600074293000},"page":"863","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Influence of Oxidative Stress Biomarkers and Genetic Polymorphisms on the Clinical Severity of Hydroxyurea-Free Senegalese Children with Sickle Cell Anemia"],"prefix":"10.3390","volume":"9","author":[{"given":"Fatou","family":"Gueye Tall","sequence":"first","affiliation":[{"name":"Laboratoire de Biochimie Pharmaceutique-FMPO, Universite Cheikh Anta Diop, Dakar BP 5005, Senegal"},{"name":"Laboratoire Interuniversitaire de Biologie de la Motricit\u00e9 (LIBM) EA7424, Equipe Biologie Vasculaire et du Globule Rouge, Universite Claude Bernard Lyon 1, COMUE Lyon, 69100 Villeurbanne, France"},{"name":"Centre Hospitalier National d\u2019Enfants Albert Royer-Dakar, Dakar BP 5005, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3378-5249","authenticated-orcid":false,"given":"Cyril","family":"Martin","sequence":"additional","affiliation":[{"name":"Centre Hospitalier National d\u2019Enfants Albert Royer-Dakar, Dakar BP 5005, Senegal"},{"name":"Laboratoire d\u2019Excellence sur le Globule Rouge (Labex GR-Ex), 75000 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El hadji Malick","family":"Ndour","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Pharmaceutique-FMPO, Universite Cheikh Anta Diop, Dakar BP 5005, Senegal"},{"name":"Centre Hospitalier National d\u2019Enfants Albert Royer-Dakar, Dakar BP 5005, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Faes","sequence":"additional","affiliation":[{"name":"Laboratoire Interuniversitaire de Biologie de la Motricit\u00e9 (LIBM) EA7424, Equipe Biologie Vasculaire et du Globule Rouge, Universite Claude Bernard Lyon 1, COMUE Lyon, 69100 Villeurbanne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indou","family":"D\u00e9me Ly","sequence":"additional","affiliation":[{"name":"Centre Hospitalier National d\u2019Enfants Albert Royer-Dakar, Dakar BP 5005, Senegal"},{"name":"Service Universitaire de P\u00e9diatrie-FMPO, Universite Cheikh Anta Diop, Dakar BP 5005, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Pialoux","sequence":"additional","affiliation":[{"name":"Laboratoire Interuniversitaire de Biologie de la Motricit\u00e9 (LIBM) EA7424, Equipe Biologie Vasculaire et du Globule Rouge, Universite Claude Bernard Lyon 1, COMUE Lyon, 69100 Villeurbanne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9232-0268","authenticated-orcid":false,"given":"Philippe","family":"Connes","sequence":"additional","affiliation":[{"name":"Laboratoire Interuniversitaire de Biologie de la Motricit\u00e9 (LIBM) EA7424, Equipe Biologie Vasculaire et du Globule Rouge, Universite Claude Bernard Lyon 1, COMUE Lyon, 69100 Villeurbanne, France"},{"name":"Laboratoire d\u2019Excellence sur le Globule Rouge (Labex GR-Ex), 75000 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Papa Madieye","family":"Gueye","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Pharmaceutique-FMPO, Universite Cheikh Anta Diop, Dakar BP 5005, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4287-9178","authenticated-orcid":false,"given":"Rokhaya","family":"Ndiaye Diallo","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Pharmaceutique-FMPO, Universite Cheikh Anta Diop, Dakar BP 5005, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Renoux","sequence":"additional","affiliation":[{"name":"Laboratoire Interuniversitaire de Biologie de la Motricit\u00e9 (LIBM) EA7424, Equipe Biologie Vasculaire et du Globule Rouge, Universite Claude Bernard Lyon 1, COMUE Lyon, 69100 Villeurbanne, France"},{"name":"UF Biochimie des Pathologies Erythrocytaires, Laboratoire de Biochimie et Biologie Moleculaire Grand-Est, Groupement Hospitalier Est, Hospices Civils de Lyon, 69500 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahima","family":"Diagne","sequence":"additional","affiliation":[{"name":"Service Universitaire de P\u00e9diatrie-FMPO, Universite Cheikh Anta Diop, Dakar BP 5005, Senegal"},{"name":"UFR des Sciences de la Sant\u00e9\u2013Universite Gaston Berger, Saint-Louis 32002, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pape Amadou","family":"Diop","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Pharmaceutique-FMPO, Universite Cheikh Anta Diop, Dakar BP 5005, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aynina","family":"Ciss\u00e9","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Pharmaceutique-FMPO, Universite Cheikh Anta Diop, Dakar BP 5005, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philom\u00e8ne Lopez","family":"Sall","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Pharmaceutique-FMPO, Universite Cheikh Anta Diop, Dakar BP 5005, Senegal"},{"name":"Centre Hospitalier National d\u2019Enfants Albert Royer-Dakar, Dakar BP 5005, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2351-9441","authenticated-orcid":false,"given":"Philippe","family":"Joly","sequence":"additional","affiliation":[{"name":"Laboratoire Interuniversitaire de Biologie de la Motricit\u00e9 (LIBM) EA7424, Equipe Biologie Vasculaire et du Globule Rouge, Universite Claude Bernard Lyon 1, COMUE Lyon, 69100 Villeurbanne, France"},{"name":"Laboratoire d\u2019Excellence sur le Globule Rouge (Labex GR-Ex), 75000 Paris, France"},{"name":"UF Biochimie des Pathologies Erythrocytaires, Laboratoire de Biochimie et Biologie Moleculaire Grand-Est, Groupement Hospitalier Est, Hospices Civils de Lyon, 69500 Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1097\/01.moh.0000219656.50291.73","article-title":"Modifier genes and sickle cell 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