{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:51:46Z","timestamp":1773287506952,"version":"3.50.1"},"reference-count":25,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T00:00:00Z","timestamp":1601078400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["APVV-19-0154"],"award-info":[{"award-number":["APVV-19-0154"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["APVV-17-0384"],"award-info":[{"award-number":["APVV-17-0384"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"publisher","award":["1\/0026\/18"],"award-info":[{"award-number":["1\/0026\/18"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"publisher","award":["2\/0053\/19"],"award-info":[{"award-number":["2\/0053\/19"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"publisher","award":["2\/0079\/19"],"award-info":[{"award-number":["2\/0079\/19"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"publisher","award":["2\/0014\/17"],"award-info":[{"award-number":["2\/0014\/17"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antioxidants"],"abstract":"<jats:p>Lipid hydroperoxides play an important role in various pathophysiological processes. Therefore, a simple model for organic hydroperoxides could be helpful to monitor the biologic effects of endogenous and exogenous compounds. The electron paramagnetic resonance (EPR) spin-trapping technique is a useful method to study superoxide (O2\u2022\u2212) and hydroxyl radicals. The aim of our work was to use EPR with the spin trap 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO), which, by trapping O2\u2022\u2212 produces relatively stable \u2022BMPO-OOH spin-adduct, a valuable model for organic hydroperoxides. We used this experimental setup to investigate the effects of selected sulfur\/selenium compounds on \u2022BMPO-OOH and to evaluate the antioxidant potential of these compounds. Second, using the simulation of time-dependent individual BMPO adducts in the experimental EPR spectra, the ratio of \u2022BMPO-OH\/\u2022BMPO-OOH\u2014which is proportional to the transformation\/decomposition of \u2022BMPO-OOH\u2014was evaluated. The order of potency of the studied compounds to alter \u2022BMPO-OOH concentration estimated from the time-dependent \u2022BMPO-OH\/\u2022BMPO-OOH ratio was as follows: Na2S4 &gt; Na2S4\/SeO32\u2212 &gt; H2S\/SeO32\u2212 &gt; Na2S2 ~Na2S2\/SeO32\u2212 ~H2S &gt; SeO32\u2212 ~SeO42\u2212 ~control. In conclusion, the presented approach of the EPR measurement of the time-dependent ratio of \u2022BMPO-OH\/\u2022BMPO-OOH could be useful to study the impact of compounds to influence the transformation of \u2022BMPO-OOH.<\/jats:p>","DOI":"10.3390\/antiox9100918","type":"journal-article","created":{"date-parts":[[2020,9,27]],"date-time":"2020-09-27T22:24:42Z","timestamp":1601245482000},"page":"918","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["\u2022BMPO-OOH Spin-Adduct as a Model for Study of Decomposition of Organic Hydroperoxides and the Effects of Sulfide\/Selenite Derivatives. An EPR Spin-Trapping Approach"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6331-1004","authenticated-orcid":false,"given":"Anton","family":"Misak","sequence":"first","affiliation":[{"name":"Department of Molecular Physiology, Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, D\u00fabravsk\u00e1 cesta 9, 84505 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vlasta","family":"Brezova","sequence":"additional","affiliation":[{"name":"Faculty of Chemical and Food Technology, Institute of Physical Chemistry and Chemical Physics, Slovak University of Technology in Bratislava, Radlinsk\u00e9ho 9, 81237 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2846-7924","authenticated-orcid":false,"given":"Marian","family":"Grman","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology, Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, D\u00fabravsk\u00e1 cesta 9, 84505 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1943-0858","authenticated-orcid":false,"given":"Lenka","family":"Tomasova","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology, Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, D\u00fabravsk\u00e1 cesta 9, 84505 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9120-5084","authenticated-orcid":false,"given":"Miroslav","family":"Chovanec","sequence":"additional","affiliation":[{"name":"Department of Genetics, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, D\u00fabravsk\u00e1 cesta 9, 84505 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8329-3563","authenticated-orcid":false,"given":"Karol","family":"Ondrias","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology, Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, D\u00fabravsk\u00e1 cesta 9, 84505 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1152\/physrev.00017.2011","article-title":"Physiological implications of hydrogen sulfide: A whiff exploration that blossomed","volume":"92","author":"Wang","year":"2012","journal-title":"Physiol. 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