{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:38:49Z","timestamp":1760150329349,"version":"build-2065373602"},"reference-count":60,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:00:00Z","timestamp":1700006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Qassim University, represented by the Deanship of Scientific Research","award":["10236-des-2020-1-3-I"],"award-info":[{"award-number":["10236-des-2020-1-3-I"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ARM"],"abstract":"<jats:p>House dust mite (HDM)-allergic asthma is an abnormal immune response to extrinsic aeroallergens found in human vicinities. Studying the role of the associated immunity biomarkers and their interplay helps in discovering novel therapeutic strategies that can be used in adjunct with effective long-term immunotherapy. This study investigates the total serum IgE, FoxO1, and Sirtuin 1 (SIRT1) gene expressions in HDM-allergic asthma patients. We enrolled 40 patients for each of the following three groups: an HV group of healthy volunteers and HDM\/AA and HDM\/SCIT groups of HDM-allergic asthma patients who did not and who did receive immunotherapy before recruitment in this study, respectively. The results elucidated that total IgE was strikingly elevated in the HDM\/AA group and showed little decline in the HDM\/SCIT group. Both FoxO1 and SIRT1 gene expressions showed the highest levels in the HDM\/SCIT group. There was a negative correlation between total IgE and both FoxO1 and SIRT1 in the HDM\/AA group while there was a positive correlation with SIRT1 in the HDM\/SCIT group. In conclusion, the interplay of the three immunity biomarkers related to HDM-allergic asthma after the course of immunotherapy treatment suggests further, broader studies on the feasibility of their role as immunity biomarkers in the control and remission of HDM-allergic asthma.<\/jats:p>","DOI":"10.3390\/arm91060039","type":"journal-article","created":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T08:19:43Z","timestamp":1700122783000},"page":"532-545","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Investigating Forkhead Box O Transcription Factor 1 Gene\u2019s Relation to Immunoglobulin E in House Dust Mite-Allergic Asthma Patients"],"prefix":"10.3390","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9540-6297","authenticated-orcid":false,"given":"Rania A.","family":"Mohamed","sequence":"first","affiliation":[{"name":"Department of Biology, Deanship of Educational Services, Qassim University, P.O. Box 5888, Unaizah 56219, Qassim, Saudi Arabia"},{"name":"Department of Parasitology, Faculty of Veterinary Medicine, Zagazig University, P.O. Box 44519, Zagazig 44516, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0497-4908","authenticated-orcid":false,"given":"Ahmed ElSadek","family":"Fakhr","sequence":"additional","affiliation":[{"name":"Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, P.O. Box 44516, Zagazig 44519, Egypt"},{"name":"Laboratory Pathology and Blood Bank, International Medical Center, P.O. Box 21589, Jeddah 23214, Makkah, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2188-6790","authenticated-orcid":false,"given":"Shereen A.","family":"Baioumy","sequence":"additional","affiliation":[{"name":"Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, P.O. Box 44516, Zagazig 44519, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ando, T., and Kitaura, J. (2021). Tuning IgE: IgE-Associating Molecules and Their Effects on IgE-Dependent Mast Cell Reactions. Cells, 10.","DOI":"10.3390\/cells10071697"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/S0091-6749(05)80006-5","article-title":"Prevalence of dust mites in the homes of people with asthma living in eight different geographic areas of the United States","volume":"90","author":"Arlian","year":"1992","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_3","unstructured":"Australasian Society of Clinical Immunology and Allergy (ASCIA) (2023, June 16). Skin Prick Testing Guide for Diagnosis of Allergic Disease. Available online: https:\/\/www.allergy.org.au\/images\/ASCIA_HP_SPT_Guide_2020.pdf."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/j.1365-2796.2012.02559.x","article-title":"Chronic rhinosinusitis and asthma: Novel understanding of the role of IgE \u2018above atopy\u2019","volume":"272","author":"Bachert","year":"2012","journal-title":"J. Intern. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s13223-021-00586-7","article-title":"Association between serum zonulin level and severity of house dust mite allergic asthma","volume":"17","author":"Baioumy","year":"2021","journal-title":"Allergy Asthma Clin. Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1034\/j.1399-3003.2000.16d07.x","article-title":"Elevation of total serum immunoglobulin E is associated with asthma in nonallergic individuals","volume":"16","author":"Beeh","year":"2000","journal-title":"Eur. Respir. J."},{"key":"ref_7","first-page":"S1","article-title":"Allergy Diagnostic Testing: An Updated Practice Parameter","volume":"100","author":"Bernstein","year":"2008","journal-title":"Ann. Allergy Asthma Immunol."},{"key":"ref_8","first-page":"149","article-title":"An understanding of the genetic basis of asthma","volume":"134","author":"Bijanzadeh","year":"2011","journal-title":"Indian J. Med. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.coi.2021.03.015","article-title":"The high affinity IgE receptor: A signaling update","volume":"72","author":"Blank","year":"2021","journal-title":"Curr. Opin. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1016\/j.jaci.2022.01.011","article-title":"Which patients with asthma are most likely to benefit from allergen immunotherapy?","volume":"149","author":"Gherasim","year":"2022","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.cellimm.2010.04.011","article-title":"IgE regulates T helper cell differentiation through Fc\u03b3RIII mediated dendritic cell cytokine modulation","volume":"264","author":"Blink","year":"2010","journal-title":"Cell. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1126\/science.1094637","article-title":"Stress-Dependent Regulation of FOXO Transcription Factors by the SIRT1 Deacetylase","volume":"303","author":"Brunet","year":"2004","journal-title":"Science"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1056\/NEJM198902023200502","article-title":"Association of Asthma with Serum IgE Levels and Skin-Test Reactivity to Allergens","volume":"320","author":"Burrows","year":"1989","journal-title":"N. Engl. J. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S1055-7903(02)00008-8","article-title":"Analysis of the mitochondrial 12S rRNA gene supports a two-clade hypothesis of the evolutionary history of scleractinian corals","volume":"23","author":"Chen","year":"2002","journal-title":"Mol. Phylogenet. Evol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7481","DOI":"10.1038\/srep07481","article-title":"High levels of SIRT1 expression enhance tumorigenesis and associate with a poor prognosis of colorectal carcinoma patients","volume":"4","author":"Chen","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1093\/advances\/nmab122","article-title":"Potential Biomarkers, Risk Factors, and Their Associations with IgE-Mediated Food Allergy in Early Life: A Narrative Review","volume":"13","author":"Childs","year":"2022","journal-title":"Adv. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/1465-9921-14-26","article-title":"Exploring factors influencing asthma control and asthma-specific health-related quality of life among children","volume":"14","author":"Gandhi","year":"2013","journal-title":"Respir. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1111\/all.13626","article-title":"FoxO1 is a critical regulator of M2-like macrophage activation in allergic asthma","volume":"74","author":"Chung","year":"2019","journal-title":"Allergy Eur. J. Allergy Clin. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1016\/j.jaci.2015.10.013","article-title":"Defective sirtuin-1 increases IL-4 expression through acetylation of GATA-3 in patients with severe asthma","volume":"137","author":"Colley","year":"2016","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1111\/cea.12450","article-title":"To what extent is allergen exposure a risk factor for the development of allergic disease?","volume":"45","author":"Custovic","year":"2015","journal-title":"Clin. Exp. Allergy"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Dilmac, S., Kuscu, N., Caner, A., Yildirim, S., Yoldas, B., Farooqi, A.A., and Tanriover, G. (2022). SIRT1\/FOXO Signaling Pathway in Breast Cancer Progression and Metastasis. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231810227"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.4049\/jimmunol.1301060","article-title":"Mst1\/Mst2 Regulate Development and Function of Regulatory T Cells through Modulation of Foxo1\/Foxo3 Stability in Autoimmune Disease","volume":"192","author":"Du","year":"2014","journal-title":"J. Immunol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"815","DOI":"10.7150\/ijbs.20052","article-title":"FOXO Signaling Pathways as Therapeutic Targets in Cancer","volume":"13","author":"Farhan","year":"2017","journal-title":"Int. J. Biol. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1183\/13993003.01824-2014","article-title":"Asthma phenotypes and IgE responses","volume":"47","author":"Froidure","year":"2016","journal-title":"Eur. Respir. J."},{"key":"ref_25","unstructured":"National Institutes of Health (2021, October 10). Global Initiative for Asthma\u2014GINA. Global Strategy for Asthma Management and Prevention Revision, Available online: https:\/\/ginasthma.org\/."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2530","DOI":"10.3389\/fimmu.2019.02530","article-title":"Mucosal Immunity and the FOXO1 Transcription Factors","volume":"10","author":"Graves","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"11844","DOI":"10.18632\/aging.102454","article-title":"Hydrogen sulfide attenuates mitochondrial dysfunction-induced cellular senescence and apoptosis in alveolar epithelial cells by upregulating sirtuin 1","volume":"11","author":"Guan","year":"2019","journal-title":"Aging"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1084\/jem.20100004","article-title":"Transcription factors Foxo3a and Foxo1 couple the E3 ligase Cbl-b to the induction of Foxp3 expression in induced regulatory T cells","volume":"207","author":"Harada","year":"2010","journal-title":"J. Exp. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3665","DOI":"10.4049\/jimmunol.1402898","article-title":"IL-10 Potentiates Differentiation of Human Induced Regulatory T Cells via STAT3 and Foxo1","volume":"195","author":"Hsu","year":"2015","journal-title":"J. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1164\/ajrccm.154.5.8912771","article-title":"IL-4 and IL-5 mRNA and protein in bronchial biopsies from patients with atopic and nonatopic asthma: Evidence against \u201cintrinsic\u201d asthma being a distinct immunopathologic entity","volume":"154","author":"Humbert","year":"1996","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1111\/j.1440-1843.2012.02284.x","article-title":"Sirtuin 1 activator SRT1720 suppresses inflammation in an ovalbumin-induced mouse model of asthma","volume":"18","author":"Ichikawa","year":"2013","journal-title":"Respirology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/03946320221114194","article-title":"Identification of potential biomarkers and immune infiltration characteristics in severe asthma","volume":"36","author":"Jiang","year":"2022","journal-title":"Int. J. Immunopathol. Pharmacol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1038\/nri914","article-title":"Regulation of mast-cell and basophil function and survival by IgE","volume":"2","author":"Kawakami","year":"2002","journal-title":"Nat. Rev. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1186\/s12967-020-02632-1","article-title":"Regulatory T cells mediated immunomodulation during asthma: A therapeutic standpoint","volume":"18","author":"Khan","year":"2020","journal-title":"J. Transl. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.jaci.2009.08.009","article-title":"Involvement of sirtuin 1 in airway inflammation and hyperresponsiveness of allergic airway disease","volume":"125","author":"Kim","year":"2010","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"6920","DOI":"10.1038\/s41598-020-63895-8","article-title":"The high protein expression of FOXO3, but not that of FOXO1, is associated with markers of good prognosis","volume":"10","author":"Lallemand","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Lee, J., Kim, J., Lee, J.H., Choi, Y.M., Choi, H., Cho, H.D., Cha, G.H., Lee, Y.H., Jo, E.K., and Park, B.H. (2022). SIRT1 Promotes Host Protective Immunity against Toxoplasma gondii by Controlling the FoxO-Autophagy Axis via the AMPK and PI3K\/AKT Signalling Pathways. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232113578"},{"key":"ref_38","first-page":"2033","article-title":"Sirt1 promotes autophagy and inhibits apoptosis to protect cardiomyocytes from hypoxic stress","volume":"43","author":"Luo","year":"2019","journal-title":"Int. J. Mol. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1165\/rcmb.2016-0356OC","article-title":"House dust mite-induced allergic airway disease is independent of IgE and FceRIa","volume":"57","author":"McKnight","year":"2017","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1038\/ncb0710-642","article-title":"Cytosolic FoxO1: Alive and killing","volume":"12","author":"Medema","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.1002\/jcp.24399","article-title":"Sirtuins\u2019 modulation of autophagy","volume":"228","author":"Ng","year":"2013","journal-title":"J. Cell. Physiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1172\/JCI8205","article-title":"IgE in asthma and atopy: Cellular and molecular connections","volume":"104","author":"Oettgen","year":"1999","journal-title":"J. Clin. Investig."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.immuni.2009.02.003","article-title":"An Essential Role of the Forkhead-Box Transcription Factor Foxo1 in Control of T Cell Homeostasis and Tolerance","volume":"30","author":"Ouyang","year":"2009","journal-title":"Immunity"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1038\/ni.1884","article-title":"Foxo proteins cooperatively control the differentiation of Foxp3+ regulatory T cells","volume":"11","author":"Ouyang","year":"2010","journal-title":"Nat. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1038\/nature11581","article-title":"Novel Foxo1-dependent transcriptional programs control Treg cell function","volume":"491","author":"Ouyang","year":"2012","journal-title":"Nature"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4700","DOI":"10.1038\/s41598-020-61455-8","article-title":"Cell-bound IgE and plasma IgE as a combined clinical diagnostic indicator for allergic patients","volume":"10","author":"Qiu","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.jaci.2015.08.005","article-title":"IgE antibodies, Fc\u03b5RI\u03b1, and IgE-mediated local anaphylaxis can limit snake venom toxicity","volume":"137","author":"Starkl","year":"2016","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1950","DOI":"10.1159\/000484119","article-title":"Suppression of Sirtuin-1 Increases IL-6 Expression by Activation of the Akt Pathway During Allergic Asthma","volume":"43","author":"Tang","year":"2017","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_49","first-page":"28","article-title":"Subcutaneous and Sublingual Immunotherapy in Allergic Asthma in Children","volume":"5","author":"Tsabouri","year":"2017","journal-title":"Front. Pediatr."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Tsilogianni, Z., Baker, J.R., Papaporfyriou, A., Papaioannou, A.I., Papathanasiou, E., Koulouris, N.G., Daly, L., Ito, K., Hillas, G., and Papiris, S. (2020). Sirtuin 1: Endocan and Sestrin 2 in Different Biological Samples in Patients with Asthma. Does Severity Make the Difference?. J. Clin. Med., 9.","DOI":"10.3390\/jcm9020473"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1097\/ACI.0b013e328344575b","article-title":"Reduction of exposure in the management of occupational asthma","volume":"11","author":"Vandenplas","year":"2011","journal-title":"Curr. Opin. Allergy Clin. Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1111\/resp.12558","article-title":"Increases in peripheral SIRT1: A new biological characteristic of asthma","volume":"20","author":"Wang","year":"2015","journal-title":"Respirology"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.3892\/mmr.2020.11338","article-title":"Suppression of sirtuin 1 alleviates airway inflammation through mTOR-mediated autophagy","volume":"22","author":"Wu","year":"2020","journal-title":"Mol. Med. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s13601-018-0220-0","article-title":"Maternal exposure to farming environment protects offspring against allergic diseases by modulating the neonatal TLR-Tregs-Th axis","volume":"8","author":"Yu","year":"2018","journal-title":"Clin. Transl. Allergy"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1080\/01902148.2018.1533051","article-title":"miR-221 participates in the airway epithelial cells injury in asthma via targeting SIRT1. Exp","volume":"44","author":"Zhang","year":"2018","journal-title":"Lung Res."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/s12931-022-01986-y","article-title":"Roles of sirtuin family members in chronic obstructive pulmonary disease","volume":"23","author":"Zhang","year":"2022","journal-title":"Respir. Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.4110\/in.2022.22.e21","article-title":"As a Modulator, Multitasking Roles of SIRT1 in Respiratory Diseases","volume":"22","author":"Zhou","year":"2022","journal-title":"Immune Netw."},{"key":"ref_58","first-page":"309","article-title":"House Dust Mite Prevalence in the House of Patients with Atopic Dermatitis in Mashhad","volume":"11","author":"Ziyaei","year":"2017","journal-title":"Iran. J Arthropod-Borne Dis."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"102034","DOI":"10.1016\/j.pupt.2021.102034","article-title":"Gentiopicroside ameliorates ovalbumin-induced airway inflammation in a mouse model of allergic asthma via regulating SIRT1\/NF-\u03baB signaling pathway","volume":"68","author":"Zou","year":"2021","journal-title":"Pulm. Pharmacol. Ther."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Biomarkers Definitions Working Group (2001). Biomarkers and surrogate endpoints: Preferred definitions and conceptual framework. Clin. Pharmacol. Ther., 69, 89\u201395.","DOI":"10.1067\/mcp.2001.113989"}],"container-title":["Advances in Respiratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2543-6031\/91\/6\/39\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:23:44Z","timestamp":1760131424000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2543-6031\/91\/6\/39"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,15]]},"references-count":60,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["arm91060039"],"URL":"https:\/\/doi.org\/10.3390\/arm91060039","relation":{},"ISSN":["2543-6031"],"issn-type":[{"type":"electronic","value":"2543-6031"}],"subject":[],"published":{"date-parts":[[2023,11,15]]}}}