{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T23:27:12Z","timestamp":1787959632686,"version":"build-2784847793"},"reference-count":0,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T00:00:00Z","timestamp":1717027200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Innovative Strategies for Prevention, Diagnosis, and Therapy of Ragweed Pollen Induced Respiratory Diseases","award":["My SMIS 103663"],"award-info":[{"award-number":["My SMIS 103663"]}]},{"name":"Innovative Strategies for Prevention, Diagnosis, and Therapy of Ragweed Pollen Induced Respiratory Diseases","award":["COP 2014-2020"],"award-info":[{"award-number":["COP 2014-2020"]}]},{"name":"Innovative Strategies for Prevention, Diagnosis, and Therapy of Ragweed Pollen Induced Respiratory Diseases","award":["92\/09.09.2016"],"award-info":[{"award-number":["92\/09.09.2016"]}]},{"name":"Innovative Strategies for Prevention, Diagnosis, and Therapy of Ragweed Pollen Induced Respiratory Diseases","award":["P_37_747"],"award-info":[{"award-number":["P_37_747"]}]},{"name":"Victor Babes University of Medicine and Pharmacy Timisoara","award":["My SMIS 103663"],"award-info":[{"award-number":["My SMIS 103663"]}]},{"name":"Victor Babes University of Medicine and Pharmacy Timisoara","award":["COP 2014-2020"],"award-info":[{"award-number":["COP 2014-2020"]}]},{"name":"Victor Babes University of Medicine and Pharmacy Timisoara","award":["92\/09.09.2016"],"award-info":[{"award-number":["92\/09.09.2016"]}]},{"name":"Victor Babes University of Medicine and Pharmacy Timisoara","award":["P_37_747"],"award-info":[{"award-number":["P_37_747"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ARM"],"abstract":"<jats:p>Ragweed pollen allergy is the most common seasonal allergy in western Romania. Prolonged exposure to ragweed pollen may induce sensitization to pan-allergens such as calcium-binding proteins (polcalcins) and progression to more severe symptoms. We aimed to detect IgE sensitization to recombinant Amb a 9 and Amb a 10 in a Romanian population, to assess their potential clinical relevance and cross-reactivity, as well as to investigate the relation with clinical symptoms. rAmb a 9 and rAmb a 10 produced in Escherichia coli were used to detect specific IgE in sera from 87 clinically characterized ragweed-allergic patients in ELISA, for basophil activation experiments and rabbit immunization. Rabbit rAmb a 9- and rAmb a 10-specific sera were used to detect possible cross-reactivity with rArt v 5 and reactivity towards ragweed and mugwort pollen extracts. The results showed an IgE reactivity of 25% to rAmb a 9 and 35% to rAmb a 10. rAmb a 10 induced basophil degranulation in three out of four patients tested. Moreover, polcalcin-negative patients reported significantly more skin symptoms, whereas polcalcin-positive patients tended to report more respiratory symptoms. Furthermore, both rabbit antisera showed low reactivity towards extracts and showed high reactivity to rArt v 5, suggesting strong cross-reactivity. Our study indicated that recombinant ragweed polcalcins might be considered for molecular diagnosis.<\/jats:p>","DOI":"10.3390\/arm92030022","type":"journal-article","created":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T12:29:47Z","timestamp":1717417787000},"page":"218-229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cross-Reactivity of Ragweed Pollen Calcium-Binding Proteins and IgE Sensitization in a Ragweed-Allergic Population from Western Romania"],"prefix":"10.3390","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1582-7740","authenticated-orcid":false,"given":"Lauriana-Eunice","family":"Zb\u00eercea","sequence":"first","affiliation":[{"name":"Center of Immuno-Physiology and Biotechnologies, Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania"},{"name":"OncoGen Center, Pius Brinzeu County Clinical Emergency Hospital, 300723 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5030-3235","authenticated-orcid":false,"given":"Maria-Roxana","family":"Buzan","sequence":"additional","affiliation":[{"name":"Center of Immuno-Physiology and Biotechnologies, Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania"},{"name":"OncoGen Center, Pius Brinzeu County Clinical Emergency Hospital, 300723 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6130-2091","authenticated-orcid":false,"given":"Manuela","family":"Grijincu","sequence":"additional","affiliation":[{"name":"Center of Immuno-Physiology and Biotechnologies, Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania"},{"name":"OncoGen Center, Pius Brinzeu County Clinical Emergency Hospital, 300723 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3034-2365","authenticated-orcid":false,"given":"Tudor-Paul","family":"Tama\u0219","sequence":"additional","affiliation":[{"name":"Center of Immuno-Physiology and Biotechnologies, Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania"},{"name":"OncoGen Center, Pius Brinzeu County Clinical Emergency Hospital, 300723 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantina Bianca","family":"Vulpe","sequence":"additional","affiliation":[{"name":"OncoGen Center, Pius Brinzeu County Clinical Emergency Hospital, 300723 Timisoara, Romania"},{"name":"Department of Chemistry-Biology, Institute for Advanced Environmental Research, West University of Timisoara, 300086 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0594-2852","authenticated-orcid":false,"given":"Ioan","family":"Hu\u021bu","sequence":"additional","affiliation":[{"name":"Horia Cernescu Research Unit, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Michael I of Romania\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virgil","family":"P\u0103unescu","sequence":"additional","affiliation":[{"name":"Center of Immuno-Physiology and Biotechnologies, Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania"},{"name":"OncoGen Center, Pius Brinzeu County Clinical Emergency Hospital, 300723 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8749-9972","authenticated-orcid":false,"given":"Carmen","family":"Panaitescu","sequence":"additional","affiliation":[{"name":"Center of Immuno-Physiology and Biotechnologies, Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania"},{"name":"OncoGen Center, Pius Brinzeu County Clinical Emergency Hospital, 300723 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5899-5766","authenticated-orcid":false,"given":"Kuan-Wei","family":"Chen","sequence":"additional","affiliation":[{"name":"OncoGen Center, Pius Brinzeu County Clinical Emergency Hospital, 300723 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,30]]},"container-title":["Advances in Respiratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2543-6031\/92\/3\/22\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:51:27Z","timestamp":1760107887000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2543-6031\/92\/3\/22"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,30]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["arm92030022"],"URL":"https:\/\/doi.org\/10.3390\/arm92030022","relation":{},"ISSN":["2543-6031"],"issn-type":[{"value":"2543-6031","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,30]]}}}