{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T04:13:59Z","timestamp":1788581639591,"version":"build-2803163510"},"reference-count":68,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T00:00:00Z","timestamp":1676246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"King Saud University, Riyadh, Saudi Arabia","award":["RSP2023R70"],"award-info":[{"award-number":["RSP2023R70"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>In recent years, green nanotechnology has gained considerable importance for the synthesis of nanoparticles due to its economic viability and biosafety. In the current study, silver nanoparticles were synthesized using two bacterial isolates, H2 and H3, which were isolated from soil samples collected from the Western Ghats, Tamil Nadu, and identified at the species level as Pantoeastewartii (H2) and Priestiaaryabhattai (H3) by sequencing their 16s rRNA genes. Intracellularly synthesized silver nanoparticles were characterized by UV\u2013visible spectroscopy, Fourier transform infrared spectroscopy, atomic force microscopy, and particle size analysis. AFM studies show that both of the bacterial synthesized Ag NPs were circular-shaped and disaggregated, with an average size distribution of 4 nm for Pantoeastewartii and 3.6 nm for Priestiaaryabhattai. Furthermore, their larvicidal activity, antimicrobial, histopathological, and biotoxicity effects were determined. The synthesized Ag NPs exhibited potent larvicidal activity against fourth instars of Ae.aegypti, An.stephensi, and Cx.quinquefasciatus exposed to a 50 \u00b5g\/mL concentration for 24 h based on their LC50 and LC90 values. Histopathological studies of the affected mosquito larvae clearly show damage to the epithelial cells, food bolus, basement membrane, muscles, and midgut parts. The maximum antimicrobial activity of Priestiaaryabhattai-synthesized Ag NPs was observed for Streptomyces varsoviensis MTCC-1537, and that of Pantoea stewartii-synthesized Ag NPs was against Escherichia coli MTCC-43. The toxicity test on non-target organisms such as Artemia nauplii and zebrafish embryos indicates no visible abnormalities or mortality after their exposure for 48h. It is concluded that silver nanoparticles can easily be synthesized using Pantoea stewartii (H2) and Priestia aryabhattai (H3) as capping and reducing agents. Silver nanoparticles showed potent larvicidal activities and could potentially be used in integrated vector control programs because they are safe for other inhabitants of the same aquatic environment as mosquito larvae.<\/jats:p>","DOI":"10.3390\/bioengineering10020248","type":"journal-article","created":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T01:41:06Z","timestamp":1676338866000},"page":"248","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Biogenic Synthesis of Silver Nanoparticles Using Pantoea stewartii and Priestia aryabhattai and Their Antimicrobial, Larvicidal, Histopathological, and Biotoxicity Potential"],"prefix":"10.3390","volume":"10","author":[{"given":"Jeyaraj John","family":"Wilson","sequence":"first","affiliation":[{"name":"Department of Microbiology, Ayya Nadar Janaki Ammal College, Sivakasi 626124, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thangamariyappan","family":"Harimuralikrishnaa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ayya Nadar Janaki Ammal College, Sivakasi 626124, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thangavel","family":"Sivakumar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ayya Nadar Janaki Ammal College, Sivakasi 626124, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunmugiah","family":"Mahendran","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ayya Nadar Janaki Ammal College, Sivakasi 626124, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ponnirul","family":"Ponmanickam","sequence":"additional","affiliation":[{"name":"Department of Zoology, Ayya Nadar Janaki Ammal College, Sivakasi 626124, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramasamy","family":"Thangaraj","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ayya Nadar Janaki Ammal College, Sivakasi 626124, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subramanian","family":"Sevarkodiyone","sequence":"additional","affiliation":[{"name":"Department of Zoology, Ayya Nadar Janaki Ammal College, Sivakasi 626124, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naiyf S.","family":"Alharbi","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shine","family":"Kadaikunnan","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3888-7079","authenticated-orcid":false,"given":"Baskar","family":"Venkidasamy","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Saveetha Dental College and Hospitals, Saveetha University, Chennai 600077, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0986-5484","authenticated-orcid":false,"given":"Muthu","family":"Thiruvengadam","sequence":"additional","affiliation":[{"name":"Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5388-5813","authenticated-orcid":false,"given":"Rajakumar","family":"Govindasamy","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 600077, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,13]]},"reference":[{"key":"ref_1","unstructured":"WHO (2015). Dengue and Severe Dengue, World Health Organization. Fact Sheet N-117."},{"key":"ref_2","unstructured":"WHO (2011, June 10). World Malaria Report 2011. Available online: http:\/\/www.who.int\/malaria\/world_malariareport."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/nrmicro2460","article-title":"Dengue: A continuing global threat","volume":"8","author":"Maria","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1007\/s00436-016-4971-z","article-title":"Declining malaria, rising of dengue and Zika virus: Insights for mosquito vector control","volume":"115","author":"Benelli","year":"2016","journal-title":"Parasitol. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/S0031-9406(05)60500-7","article-title":"Management of patients with lymphoedema caused by filariasis in North-eastern Tanzania","volume":"89","author":"Lise","year":"2003","journal-title":"Physiotherapy"},{"key":"ref_7","unstructured":"WHO (2005). Guidelines for Laboratory and Field Testing of Mosquito Larvicides, World Health Organization. WHO\/CDS\/WHOPES\/GCDPP\/13."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.ibmb.2012.10.006","article-title":"Impact of environment on mosquito response to pyrethroid insecticides: Facts, evidences and prospects","volume":"43","author":"Theresia","year":"2013","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12934-016-0422-x","article-title":"Microbial glycolipoprotein-capped silver nanoparticles as emerging antibacterial agents against cholera","volume":"15","author":"Gahlawat","year":"2016","journal-title":"Microb. Cell Fact."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"65101","DOI":"10.1088\/1361-6528\/abc2eb","article-title":"Edible mushroom (Flammulinavelutipes) as biosource for silver nanoparticles: From synthesis to diverse biomedical and environmental applications","volume":"32","author":"Faisal","year":"2021","journal-title":"Nanotechnology"},{"key":"ref_11","first-page":"385","article-title":"Synthesis of silver nanoparticles: Chemical, physical and biological methods","volume":"9","author":"Iravani","year":"2014","journal-title":"Res. Pharm. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.arabjc.2018.05.008","article-title":"Green synthesis of platinum nanoparticles using Saudi\u2019s Dates extract and their usage on the cancer cell treatment","volume":"12","year":"2019","journal-title":"Arab. J. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1166\/jctn.2018.7317","article-title":"Artichoke (Cynara scolymus L.,) mediated rapid analysis of silver nanoparticles and their utilisation on the cancer cell treatments","volume":"15","year":"2018","journal-title":"J. Comput. Theor. Nanosci."},{"key":"ref_14","first-page":"1","article-title":"Green Biosynthesis of Flaxseed Gold Nanoparticles (Au-NPs) as Potent Anticancer Agent against Breast Cancer Cells","volume":"25","year":"2021","journal-title":"J. Saudi Chem. Soc."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1016\/j.arabjc.2014.12.014","article-title":"Synthesis of silver nanoparticles with different shapes","volume":"12","author":"Khodashenas","year":"2019","journal-title":"Arab. J. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.2147\/IJN.S58932","article-title":"Biosynthesis and characterization of silver nanoparticles using panchakavya, an Indian traditional farming formulating agent","volume":"9","author":"Govarthanan","year":"2014","journal-title":"Int. J. Nanomed."},{"key":"ref_17","first-page":"1977","article-title":"Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects","volume":"10","author":"Aravinthan","year":"2015","journal-title":"Int. J. Nanomed."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3095","DOI":"10.1007\/s11164-015-2199-7","article-title":"Green synthesis of silver nanoparticles using Solanum indicum L. and their antibacterial, splenocyte cytotoxic potentials","volume":"42","author":"Sengottaiyan","year":"2015","journal-title":"Res. Chem. Intermediat."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"127233","DOI":"10.1016\/j.molstruc.2019.127233","article-title":"Soil bacteria Cupriavidus sp. mediates the extracellular synthesis of antibacterial silver nanoparticles","volume":"1202","author":"Ameen","year":"2020","journal-title":"J. Mol. Struct."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.3109\/21691401.2015.1111230","article-title":"Low-cost and eco-friendly synthesis of silver nanoparticles using coconut (Cocos nucifera) oil cake extract and its antibacterial activity","volume":"44","author":"Govarthanan","year":"2016","journal-title":"Artif. Cell Nanomed. Biotechnol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.toxrep.2020.04.011","article-title":"Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies","volume":"7","author":"Varmazyari","year":"2020","journal-title":"Toxicol. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1007\/s12034-021-02435-0","article-title":"Bio fabrication of gold nanoparticles using Ganoderma lucidum and their cytotoxicity against human colon cancer cell line (HT-29)","volume":"44","author":"Elumalai","year":"2021","journal-title":"Bull. Mater. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"19459","DOI":"10.1007\/s11356-017-9772-0","article-title":"Biosynthesis of silver nanoparticles from Spirulina microalgae and its antibacterial activity","volume":"24","author":"Govarthanan","year":"2017","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.matlet.2018.04.111","article-title":"Utilization of market vegetable waste for silver nanoparticle synthesis and its antibacterial activity","volume":"225","author":"Mythili","year":"2018","journal-title":"Mater. Lett."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"127244","DOI":"10.1016\/j.matlet.2019.127244","article-title":"Utilization of marine seaweed Spyridia filamentosa for silver nanoparticles synthesis and its clinical applications","volume":"263","author":"Valarmathi","year":"2020","journal-title":"Mater. Lett."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5822","DOI":"10.1021\/cm801475v","article-title":"Polyaminoacid-induced growth of metal nanoparticles on layer-by-Layer templates","volume":"20","author":"Eugenia","year":"2008","journal-title":"Chem. Mater."},{"key":"ref_27","unstructured":"Helms, D.R., Helms, C.W., Kosinski, R.J., and Cummings, J.R. (1998). Biology in the Laboratory, W. H. Freeman. [3rd ed.]."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.3389\/fmicb.2018.01129","article-title":"Biogenic Synthesis of Novel Functionalized Selenium Nanoparticles by Lactobacillus casei ATCC 393 and Its Protective Effects on Intestinal Barrier Dysfunction Caused by Enterotoxigenic Escherichia coli K88","volume":"9","author":"Xu","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.enzmictec.2016.08.018","article-title":"Intracellular synthesis of gold nanoparticles with antioxidant activity by probiotic Lactobacillus kimchicus DCY51T isolated from Korean kimchi","volume":"95","author":"Markus","year":"2016","journal-title":"Enzym. Microb. Technol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s12934-020-1279-6","article-title":"Sustainable microbial cell nanofactory for zinc oxide nanoparticles production by zinc-tolerant probiotic Lactobacillus plantarum strain TA4. Microb","volume":"19","author":"Mohamad","year":"2020","journal-title":"Cell Factories"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1007\/s00253-009-2032-6","article-title":"Lactic acid bacteria as reducing and capping agent for the fast and efficient production of silver nanoparticles","volume":"84","author":"Sintubin","year":"2009","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.colsurfa.2018.05.069","article-title":"Mechanism study of intracellular zinc oxide nanocomposites formation","volume":"553","author":"Pomastowski","year":"2018","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Teleanu, D.M., Negut, I., Grumezescu, V., Grumezescu, A.M., and Teleanu, R.I. (2019). Nanomaterials for drug delivery to the central nervous system. Nanomaterials, 9.","DOI":"10.3390\/nano9030371"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/829526","article-title":"Biosynthesis of silver nanoparticles and its applications","volume":"2015","author":"Firdhouse","year":"2015","journal-title":"J. Nanotechnol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/j.carbpol.2016.03.029","article-title":"Antibacterial activity of silver nanoparticles synthesized In-situ by solution spraying onto cellulose","volume":"147","author":"Yan","year":"2016","journal-title":"Carbohydr. Polym."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Paladini, F., and Pollini, M. (2019). Antimicrobial Silver Nanoparticles for Wound Healing Application: Progress and Future Trends. Materials, 12.","DOI":"10.3390\/ma12162540"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1590\/fst.36318","article-title":"Application of silver nanoparticles in food packages: A review","volume":"39","author":"Simbine","year":"2019","journal-title":"Food Sci. Technol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.3389\/fmicb.2016.01831","article-title":"Mechanistic Basis of Antimicrobial Actions of Silver Nanoparticles","volume":"7","author":"Dakal","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.1007\/s00253-011-3249-8","article-title":"Mechanistic aspects in the biogenic synthesis of extracellular metal nanoparticles by peptides, bacteria, fungi, and plants","volume":"90","author":"Duran","year":"2011","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s10876-018-1478-z","article-title":"Larvicidal activity of silver nanoparticles synthesized by Pseudomonas fluorescens YPS3 isolated from the Eastern Ghats of India","volume":"30","author":"Kalaimurugan","year":"2019","journal-title":"J. Clust. Sci."},{"key":"ref_41","first-page":"1","article-title":"Bacillus aryabhattai SRB02 tolerates oxidative and nitrosative stress and promotes the growth of soybean by modulating the production of phytohormones","volume":"12","author":"Park","year":"2017","journal-title":"PLoS ONE"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s13205-013-0130-8","article-title":"Extracellular synthesis of silver nanoparticles by the Bacillus strain CS11 isolated from industrialized area","volume":"4","author":"Das","year":"2014","journal-title":"3 Biotech"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1007\/s00436-014-4268-z","article-title":"Antimicrobial and mosquitocidal activity of microbial synthesized silver nanoparticles","volume":"114","author":"Soni","year":"2015","journal-title":"Parasitol. Res."},{"key":"ref_44","first-page":"4637325","article-title":"Silver nanoparticles synthesized through green methods using Escherichia coli top 10 (Ec-Ts) growth culture medium exhibit antimicrobial properties against nongrowing bacterial Strains","volume":"2019","year":"2019","journal-title":"J. Nanomater."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.bcab.2018.07.006","article-title":"Biocatalysis and agricultural biotechnology antimicrobial and larvicidal activity of eco-friendly silver nanoparticles synthesized from endophytic fungi Phomopsis liquidambaris","volume":"16","author":"Kumar","year":"2018","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s40089-014-0133-4","article-title":"Green synthesis of silver nanoparticles using keratinase obtained from a strain of Bacillus safensis LAU 13","volume":"5","author":"Lateef","year":"2015","journal-title":"Int. Nano Lett."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Monowar, T., Rahman, M., Bhore, S.J., Raju, G., and Sathasivam, K.V. (2018). Silver nanoparticles synthesized by using the endophytic bacterium Pantoea ananatis are promising antimicrobial agents against multidrug resistant bacteria. Molecules, 23.","DOI":"10.3390\/molecules23123220"},{"key":"ref_48","first-page":"39","article-title":"Synthesis and characterization of silver nanoparticle from Erythrina indica","volume":"7","author":"Kalainila","year":"2014","journal-title":"Asian J. Pharm. Clin. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s40097-018-0256-7","article-title":"Biosynthesis of silver nanoparticles using stem bark extracts of Diospyros montana and their antioxidant and antibacterial activities","volume":"8","author":"Bharathi","year":"2018","journal-title":"J. Nanostructure Chem."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Shamaila, S., Zafar, N., Riaz, S., Sharif, R., Nazir, J., and Naseem, S. (2016). Gold nanoparticles: An efficient antimicrobial agent against enteric bacterial human pathogen. Nanomaterials, 6.","DOI":"10.3390\/nano6040071"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.btre.2017.02.006","article-title":"Synthesis of silver nanoparticles using bacterial exopolysaccharide and its application for degradation of azo-dyes","volume":"15","author":"Saravanan","year":"2017","journal-title":"Biotechnol. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1007\/s00436-013-3656-0","article-title":"Biosynthesis of silver nanoparticles using Bacillus thuringiensis against dengue vector, Aedes aegypti (Diptera: Culicidae)","volume":"113","author":"Banu","year":"2013","journal-title":"Parasitol. Res."},{"key":"ref_53","first-page":"2394","article-title":"Biosynthesis of silver nanoparticles for mosquito control","volume":"4","author":"Rajesh","year":"2017","journal-title":"Br. J. Med. Health Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.jphotobiol.2017.06.004","article-title":"Biopolymer zein-coated gold nanoparticles: Synthesis, antibacterial potential, toxicity and histopathological effects against the Zika virus vector Aedes aegypti","volume":"173","author":"Suganya","year":"2017","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1080\/21691401.2017.1365723","article-title":"Larvicidal and pupicidal evaluation of silver nanoparticles synthesized using Aquilaria sinensis and Pogostemon cablin essential oils against dengue and zika viruses vector Aedes albopictus mosquito and its histopathological analysis","volume":"46","author":"Fouad","year":"2018","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.psep.2017.03.027","article-title":"Control of dengue and Zika virus vector Aedes aegypti using the predatory copepod Megacyclops formosanus: Synergy with Hedychium coronarium-synthesized silver nanoparticles and related histological changes in targeted mosquitoes","volume":"109","author":"Kalimuthu","year":"2017","journal-title":"Process Saf. Environ. Prot."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"256919","DOI":"10.1155\/2014\/256919","article-title":"Toxicity effect of silver nanoparticles in brine shrimp Artemia","volume":"2014","author":"Arulvasu","year":"2014","journal-title":"Sci. World J."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.nano.2012.04.007","article-title":"Enhanced antimicrobial activity of silver nanoparticles synthesized by Cryphonectria sp. evaluated singly and in combination with antibiotics","volume":"9","author":"Dar","year":"2013","journal-title":"Nanomed. Nanotechnol. Biol. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"255102","DOI":"10.1088\/0957-4484\/19\/25\/255102","article-title":"Toxicity of silver nanoparticles in zebrafish models","volume":"19","author":"Asharani","year":"2008","journal-title":"Nanotechnology"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"105263","DOI":"10.1016\/j.aquatox.2019.105263","article-title":"Silver nanoparticle exposure impairs ion regulation in zebrafish embryos","volume":"214","author":"Lee","year":"2019","journal-title":"Aquat. Toxicol."},{"key":"ref_61","unstructured":"Cappuccino, J.G., and Sherman, N. (2014). Microbiology: A Laboratory Manual, Pearson\/Benjamin Cummings Pub. Co. Inc.. [10th ed.]."},{"key":"ref_62","first-page":"25","article-title":"Antimicrobial activity of extracellularly synthesized silver nanoparticles using Lactobacillus species obtained from VIZYLAC capsule","volume":"2","author":"Chaudhari","year":"2012","journal-title":"J. Appl. Pharm. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Rai, M., and Duran, N. (2011). Metal Nanoparticles in Microbiology, Springer. [1st ed.].","DOI":"10.1007\/978-3-642-18312-6"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5958\/0974-4576.2021.00001.3","article-title":"Mosquito larvicidal activity of synthesized silver nanoparticles from selected bacteria against Aedes aegypti","volume":"45","author":"Wilson","year":"2021","journal-title":"J. Entomol. Res."},{"key":"ref_65","first-page":"2533","article-title":"Characterization and optimization of AgNPs biosynthesis by Klebsiella pneumoniae HHHS1 isolated from silver rich environment","volume":"18","author":"Hassan","year":"2018","journal-title":"Biochem. Cell. Arch."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"773","DOI":"10.3389\/fphar.2017.00773","article-title":"Larvicidal, histopathological efficacy of Penicillium daleae against larvae of Culex quinquefasciatus and Aedes aegypti plus biotoxicity on Artemia nauplii a non-target aquatic organism","volume":"8","author":"Ragavendran","year":"2017","journal-title":"Front. Pharmacol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1016\/j.sjbs.2020.05.008","article-title":"Dye degradation, antimicrobial and larvicidal activity of silver nanoparticles biosynthesized from Cleistanthus collinus","volume":"27","author":"Lourthuraj","year":"2020","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"115101","DOI":"10.1088\/0957-4484\/24\/11\/115101","article-title":"Silver nanoparticle toxicity in the embryonic Zebrafish is governed by particle dispersion and ionic environment","volume":"24","author":"Kim","year":"2013","journal-title":"Nanotechnology"}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/10\/2\/248\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:33:56Z","timestamp":1760121236000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/10\/2\/248"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,13]]},"references-count":68,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["bioengineering10020248"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering10020248","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,13]]}}}