{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:38:04Z","timestamp":1790735884085,"version":"4.1.0"},"reference-count":65,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T00:00:00Z","timestamp":1682553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"Ministry of Education","doi-asserted-by":"publisher","award":["2022R1I1A1A01071220"],"award-info":[{"award-number":["2022R1I1A1A01071220"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Ministry of Education","doi-asserted-by":"publisher","award":["2020R1A5A1018052"],"award-info":[{"award-number":["2020R1A5A1018052"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"Ministry of Education","doi-asserted-by":"publisher","award":["20021979"],"award-info":[{"award-number":["20021979"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Korean government (MSIT)","award":["2022R1I1A1A01071220"],"award-info":[{"award-number":["2022R1I1A1A01071220"]}]},{"name":"Korean government (MSIT)","award":["2020R1A5A1018052"],"award-info":[{"award-number":["2020R1A5A1018052"]}]},{"name":"Korean government (MSIT)","award":["20021979"],"award-info":[{"award-number":["20021979"]}]},{"name":"Technology Innovation Program","award":["2022R1I1A1A01071220"],"award-info":[{"award-number":["2022R1I1A1A01071220"]}]},{"name":"Technology Innovation Program","award":["2020R1A5A1018052"],"award-info":[{"award-number":["2020R1A5A1018052"]}]},{"name":"Technology Innovation Program","award":["20021979"],"award-info":[{"award-number":["20021979"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>A diabetic wound presents a severe risk of infections and other complications because of its slow healing. Evaluating the pathophysiology during wound healing is imperative for wound care, necessitating a proper diabetic wound model and assay for monitoring. The adult zebrafish is a rapid and robust model for studying human cutaneous wound healing because of its fecundity and high similarities to human wound repair. OCTA as an assay can provide three-dimensional (3D) imaging of the tissue structure and vasculature in the epidermis, enabling monitoring of the pathophysiologic alterations in the zebrafish skin wound. We present a longitudinal study for assessing the cutaneous wound healing of the diabetic adult zebrafish model using OCTA, which is of importance for the diabetes research using the alternative animal models. We used non-diabetic (n = 9) and type 1 diabetes mellitus (DM) adult zebrafish models (n = 9). The full-thickness wound was generated on the fish skin, and the wound healing was monitored with OCTA for 15 days. The OCTA results demonstrated significant differences between diabetic and non-diabetic wound healing, involving delayed tissue remodeling and impaired angiogenesis for the diabetic wound, leading to slow wound recovery. The adult zebrafish model and OCTA technique may benefit long-term metabolic disease studies using zebrafish for drug development.<\/jats:p>","DOI":"10.3390\/bioengineering10050538","type":"journal-article","created":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T02:02:23Z","timestamp":1682647343000},"page":"538","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Non-Invasive Monitoring of Cutaneous Wound Healing in Non-Diabetic and Diabetic Model of Adult Zebrafish Using OCT Angiography"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8889-6953","authenticated-orcid":false,"given":"Jaeyoung","family":"Kim","sequence":"first","affiliation":[{"name":"Research Institute for Skin Image, Korea University College of Medicine, Seoul 08308, Republic of Korea"},{"name":"Department of Dermatology and Skin Science, University of British Columbia, Vancouver, BC V6T 1Z1, Canada"},{"name":"Departments of Cancer Control Research and Integrative Oncology, British Columbia Cancer Agency, Vancouver, BC V5Z 1L3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7425-1310","authenticated-orcid":false,"given":"Suhyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Korea University College of Medicine, Seoul 02841, Republic of Korea"},{"name":"Zebrafish Translational Medical Research Center, Korea University Ansan Hospital, Ansan 15355, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0793-2735","authenticated-orcid":false,"given":"Woo June","family":"Choi","sequence":"additional","affiliation":[{"name":"School of Electrical and Electronics Engineering, Chung-Ang University, Seoul 06974, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"American Diabetes Association (2010). Diagnosis and Classification of Diabetes Mellitus. Diabetes Care, 33, S62\u2013S69.","DOI":"10.2337\/dc10-S062"},{"key":"ref_2","unstructured":"International Diabetes Federation (2022, November 29). IDF Diabetes Atlas 2021: IDF Diabetes Atlas 10th Edition. Available online: https:\/\/diabetesatlas.org\/atlas\/tenth-edition\/."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1111\/j.2040-1124.2010.00070.x","article-title":"Mechanism of diabetic neuropathy: Where are we now and where to go?","volume":"2","author":"Yagihashi","year":"2011","journal-title":"J. Diabetes Investig."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Weledji, E.P., and Fokam, P. (2014). Treatment of the diabetic foot\u2013to amputate or not?. BMC Surg., 14.","DOI":"10.1186\/1471-2482-14-83"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1056\/NEJM199909023411006","article-title":"Cutaneous Wound Healing","volume":"341","author":"Singer","year":"1999","journal-title":"N. Engl. J. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1177\/147323000903700531","article-title":"The Wound Healing Process: An Overview of the Cellular and Molecular Mechanisms","volume":"37","author":"Velnar","year":"2009","journal-title":"J. Int. Med. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"265sr6","DOI":"10.1126\/scitranslmed.3009337","article-title":"Wound repair and regeneration: Mechanisms, signaling, and translation","volume":"6","author":"Eming","year":"2014","journal-title":"Sci. Transl. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1067-1927.2005.130102.x","article-title":"Tissue repair, contraction, and the myofibroblast","volume":"13","author":"Chaponnier","year":"2005","journal-title":"Wound Repair Regen."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1038\/sj.jid.5700613","article-title":"Formation and Function of the Myofibroblast during Tissue Repair","volume":"127","author":"Hinz","year":"2007","journal-title":"J. Investig. Dermatol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1111\/wrr.12937","article-title":"Myofibroblast progeny in wound biology and wound healing studies","volume":"29","author":"Monika","year":"2021","journal-title":"Wound Repair Regen."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"791899","DOI":"10.3389\/fnut.2021.791899","article-title":"Challenges in Healing Wound: Role of Complementary and Alternative Medicine","volume":"8","author":"Monika","year":"2021","journal-title":"Front. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/iep.12346","article-title":"Experimental models and methods for cutaneous wound healing assessment","volume":"101","author":"Andrade","year":"2020","journal-title":"Int. J. Exp. Pathol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1016\/j.jid.2018.08.005","article-title":"Research Techniques Made Simple: Animal Models of Wound Healing","volume":"138","author":"Grada","year":"2018","journal-title":"J. Investig. Dermatol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1038\/jid.2013.16","article-title":"Adult Zebrafish as a Model System for Cutaneous Wound-Healing Research","volume":"133","author":"Richardson","year":"2013","journal-title":"J. Investig. Dermatol."},{"key":"ref_15","first-page":"2077","article-title":"Re-epithelialization of cutaneous wounds in adult zebrafish combines mechanisms of wound closure in embryonic and adult mammals","volume":"15","author":"Richardson","year":"2016","journal-title":"Development"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"R113","DOI":"10.1186\/gb-2013-14-10-r113","article-title":"The genetic heterogeneity and mutational burden of engineered melanomas in zebrafish models","volume":"14","author":"Yen","year":"2013","journal-title":"Genome Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1089\/zeb.2006.3.235","article-title":"Modulatory Neurotransmitter Systems and Behavior: Towards Zebrafish Models of Neurodegenerative Diseases","volume":"3","author":"Panula","year":"2006","journal-title":"Zebrafish"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.aanat.2015.11.003","article-title":"Morphological differences in adipose tissue and changes in BDNF\/Trkb expression in brain and gut of a diet induced obese zebrafish model","volume":"204","author":"Montalbano","year":"2016","journal-title":"Ann. Anat."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Tsuji, N., Ninov, N., Delawary, M., Osman, S., Roh, A.S., Gut, P., and Stainier, D.Y.R. (2014). Whole Organism High Content Screening Identifies Stimulators of Pancreatic Beta-Cell Proliferation. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0104112"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/s41536-018-0059-y","article-title":"Parallels between vertebrate cardiac and cutaneous wound healing and regeneration","volume":"3","author":"Richardson","year":"2018","journal-title":"NPJ Regen. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s10456-018-09660-y","article-title":"Live imaging of angiogenesis during cutaneous wound healing in adult zebrafish","volume":"22","author":"Noishiki","year":"2019","journal-title":"Angiogenesis"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1109\/2944.796348","article-title":"Optical coherence tomography (OCT): A review","volume":"5","author":"Schmitt","year":"1999","journal-title":"IEEE J. Sel. Top. Quantum Electron."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1088\/0031-9155\/49\/7\/002","article-title":"The potential of optical coherence tomography in the engineering of living tissue","volume":"49","author":"Mason","year":"2004","journal-title":"Phys. Med. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3407","DOI":"10.1364\/BOE.389342","article-title":"Nanosensitive optical coherence tomography to assess wound healing within the cornea","volume":"11","author":"Lal","year":"2020","journal-title":"Biomed. Opt. Express"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e202000357","DOI":"10.1002\/jbio.202000357","article-title":"Attenuationcorrected-opticalcoherence tomography for quantitative assessment of skin wound healing and scar morphology","volume":"14","author":"Ghosh","year":"2020","journal-title":"J. Biophotonics"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1038\/nm.1971","article-title":"Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging","volume":"15","author":"Vakoc","year":"2009","journal-title":"Nat. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.preteyeres.2017.11.003","article-title":"Optical coherence tomography angiography","volume":"64","author":"Spaide","year":"2018","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"135","DOI":"10.21037\/qims.2018.02.07","article-title":"Optical coherence tomography angiography monitors human cutaneous wound healing over time","volume":"8","author":"Deegan","year":"2018","journal-title":"Quant. Imaging Med. Surg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1111\/exd.13053","article-title":"Optical coherence tomography angiography for longitudinal monitoring of vascular changes in human cutaneous burns","volume":"25","author":"Gong","year":"2016","journal-title":"Exp. Dermatol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"061213","DOI":"10.1117\/1.JBO.18.6.061213","article-title":"In vivo assessment of human burn scars through automated quantification of vascularity using optical coherence tomography","volume":"18","author":"Liew","year":"2013","journal-title":"J. Biomed. Opt."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e001479","DOI":"10.1136\/bmjdrc-2020-001479","article-title":"Optical coherence tomography: A novel imaging approach to visualize and quantify cutaneous microvascular structure and function in patients with diabetes","volume":"8","author":"Argarini","year":"2020","journal-title":"BMJ Open Diabetes Res. Care"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.2522\/ptj.20080008","article-title":"Diabetes-related microvascular and macrovascular diseases in the physical therapy setting","volume":"88","author":"Cade","year":"2008","journal-title":"Phys. Ther."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1038\/s41598-022-26871-y","article-title":"Pilot study of optical coherence tomography angiography-derived microvascular metrics in hands and feet of healthy and diabetic people","volume":"13","author":"Untracht","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_34","unstructured":"Rieger, S. (2022, December 15). Ablation of \u03b2-Cells in Tg(ins:NTR mCherry) Transgenic Zebrafish Using Metronidazole. Available online: https:\/\/www.diacomp.org\/shared\/document.aspx?id=221&docType=Protocol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1002\/dvdy.21100","article-title":"Conditional targeted cell ablation in zebrafish: A new tool for regeneration studies","volume":"236","author":"Curado","year":"2007","journal-title":"Dev. Dyn."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1097\/POC.0000000000000125","article-title":"Type 1 Diabetes\u2014A Clinical Perspective","volume":"16","author":"Kahanovitz","year":"2017","journal-title":"Point Care"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"557","DOI":"10.21037\/qims.2016.10.03","article-title":"Highly efficient eigen decomposition based statistical optical microangiography","volume":"6","author":"Zhang","year":"2016","journal-title":"Quant. Imaging Med. Surg."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.jneumeth.2016.06.014","article-title":"Automated segmentation and enhancement of optical coherence tomography-acquired images of rodent brain","volume":"270","author":"Baran","year":"2016","journal-title":"J. Neurosci. Methods"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e1056","DOI":"10.31661\/gmj.v8i0.1056","article-title":"Generation of a Transgenic Zebrafish Model for Pancreatic Beta Cell Regeneration","volume":"8","author":"Pourghadamyari","year":"2019","journal-title":"Galen Med. J."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e23","DOI":"10.1017\/S1462399411001943","article-title":"Inflammation and wound healing: The role of the macrophage","volume":"13","author":"Koh","year":"2011","journal-title":"Expert Rev. Mol. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"17","DOI":"10.4049\/jimmunol.1700223","article-title":"Macrophage-Mediated Inflammation in Normal and Diabetic Wound Healing","volume":"199","author":"Boniakowski","year":"2017","journal-title":"J. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2446","DOI":"10.1111\/raq.12443","article-title":"Mechanical induced wounds in fish\u2014A review on models and healing mechanisms","volume":"12","author":"Sveen","year":"2020","journal-title":"Rev. Aquac."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1089\/wound.2013.0517","article-title":"Vascular Endothelial Growth Factor and Angiogenesis in the Regulation of Cutaneous Wound Repair","volume":"3","author":"Johnson","year":"2014","journal-title":"Adv. Wound Care"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Okonkwo, U.A., and DiPietro, L.A. (2017). Diabetes and Wound Angiogenesis. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18071419"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Okonkwo, U.A., Chen, L., Ma, D., Haywood, V.A., Barakat, M., Urao, N., and DiPietro, L.A. (2020). Compromised angiogenesis and vascular Integrity in impaired diabetic wound healing. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0231962"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Pfister, M., Sch\u00fctzenberger, K., Sch\u00e4fer, B.J., Puchner, S., Stegmann, H., Hohenadl, C., Mildner, M., Garh\u00f6fer, G., Schmetterer, L., and Werkmeister, R.M. (2021). Optical Coherence Tomography Angiography Monitors Cutaneous Wound Healing under Angiogenesis-Promoting Treatment in Diabetic and Non-Diabetic Mice. Appl. Sci., 11.","DOI":"10.3390\/app11052447"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1844","DOI":"10.2337\/db08-0628","article-title":"Regeneration of the Pancreas in Adult Zebrafish","volume":"58","author":"Moss","year":"2009","journal-title":"Diabetes"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1364\/BOE.8.001056","article-title":"Optical coherence tomography based angiography [Invited]","volume":"8","author":"Chen","year":"2017","journal-title":"Biomed. Opt. Express"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1364\/BOE.9.001244","article-title":"Quantitative biometry of zebrafish retinal vasculature using optical coherence tomographic angiography","volume":"9","author":"Bozic","year":"2018","journal-title":"Biomed. Opt. Express"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2150022","DOI":"10.1142\/S179354582150022X","article-title":"High-resolution polarization-sensitive optical coherence tomography and optical coherence tomography angiography for zebrafish skin imaging","volume":"14","author":"Yang","year":"2021","journal-title":"J. Innov. Opt. Health Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"016001","DOI":"10.1117\/1.JBO.27.1.016001","article-title":"Multicontrast investigation of in vivo wildtype zebrafish in three development stages using polarization-sensitive optical coherence tomography","volume":"27","author":"Lichtenegger","year":"2022","journal-title":"J. Biomed. Opt."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"025601","DOI":"10.1088\/1555-6611\/aa995f","article-title":"Feasibility of speckle variance OCT for imaging cutaneous microvasculature regeneration during healing of wounds in diabetic mice","volume":"28","author":"Sharma","year":"2018","journal-title":"Laser Phys."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e000974","DOI":"10.1136\/bmjdrc-2019-000974","article-title":"Non-invasive monitoring of pharmacodynamics during the skin wound healing process using multimodal optical microscopy","volume":"8","author":"Lee","year":"2020","journal-title":"BMJ Open Diabetes Res. Care"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e201900076","DOI":"10.1002\/jbio.201900076","article-title":"Dynamic imaging and quantification of subcellular motion with eigen-decomposition optical coherence tomography-based variance analysis","volume":"12","author":"Wei","year":"2019","journal-title":"J. Biophotonics"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.1016\/S0002-9440(10)63754-6","article-title":"Topical Vascular Endothelial Growth Factor Accelerates Diabetic Wound Healing through Increased Angiogenesis and by Mobilizing and Recruiting Bone Marrow-Derived Cells","volume":"164","author":"Galiano","year":"2004","journal-title":"Am. J. Pathol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1215\/S1152851705000232","article-title":"The role of pericytes in blood-vessel formation and maintenance","volume":"7","author":"Bergers","year":"2005","journal-title":"Neuro Oncol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1080\/01926230600936290","article-title":"Review of the Pericyte during Angiogenesis and its Role in Cancer and Diabetic Retinopathy","volume":"34","author":"Hall","year":"2006","journal-title":"Toxicol. Pathol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1038\/nature07039","article-title":"Wound repair and regeneration","volume":"453","author":"Gurtner","year":"2008","journal-title":"Nature"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1016\/j.jacc.2004.11.067","article-title":"Circulating Endothelial Progenitor Cells Are Reduced in Peripheral Vascular Complications of Type 2 Diabetes Mellitus","volume":"45","author":"Fadini","year":"2005","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.mod.2006.11.005","article-title":"Targeted ablation of beta cells in the embryonic zebrafish pancreas using E. coli nitroreductase","volume":"124","author":"Pisharath","year":"2007","journal-title":"Mech. Dev."},{"key":"ref_61","first-page":"319","article-title":"Genetic and chemically-induced Zebrafish models for the study of diabetes mellitus","volume":"5","author":"Sarras","year":"2018","journal-title":"MOJ Anat. Physiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1111\/dgd.12565","article-title":"Zebrafish as a model for studying functional pancreatic \u03b2 cells development and regeneration","volume":"60","author":"Matsuda","year":"2018","journal-title":"Dev. Growth Differ."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"5037","DOI":"10.1364\/OL.44.005037","article-title":"Electrically tunable lens integrated with optical coherence tomography angiography for cerebral blood flow imaging in deep cortical layers in mice","volume":"44","author":"Li","year":"2019","journal-title":"Opt. Lett."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Untracht, G.R., Matos, R.S., Dikaios, N., Bapir, M., Durrani, A.K., Butsabong, T., Campagnolo, P., Sampson, D.D., Heiss, C., and Sampson, D.M. (2021). OCTAVA: An open-source toolbox for quantitative analysis of optical coherence tomography angiography images. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0261052"},{"key":"ref_65","unstructured":"Armi, L., and Fekri-Ershad, S. (2019). Texture image analysis and texture classification methods\u2014A review. arXiv."}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/10\/5\/538\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:24:40Z","timestamp":1760124280000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/10\/5\/538"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,27]]},"references-count":65,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2023,5]]}},"alternative-id":["bioengineering10050538"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering10050538","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,27]]}}}