{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:03:45Z","timestamp":1792015425524,"version":"4.4.0"},"reference-count":48,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,28]],"date-time":"2023-05-28T00:00:00Z","timestamp":1685232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation (DFG)","doi-asserted-by":"publisher","award":["346772917"],"award-info":[{"award-number":["346772917"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Monoclonal antibodies are increasingly dominating the market for human therapeutic and diagnostic agents. For this reason, continuous methods\u2014such as perfusion processes\u2014are being explored and optimized in an ongoing effort to increase product yields. Unfortunately, many established cell retention devices\u2014such as tangential flow filtration\u2014rely on membranes that are prone to clogging, fouling, and undesirable product retention at high cell densities. To circumvent these problems, in this work, we have developed a 3D-printed microfluidic spiral separator for cell retention, which can readily be adapted and replaced according to process conditions (i.e., a plug-and-play system) due to the fast and flexible 3D printing technique. In addition, this system was also expanded to include automatic flushing, web-based control, and notification via a cellphone application. This set-up constitutes a proof of concept that was successful at inducing a stable process operation at a viable cell concentration of 10\u201317 \u00d7 106 cells\/mL in a hybrid mode (with alternating cell retention and cell bleed phases) while significantly reducing both shear stress and channel blockage. In addition to increasing efficiency to nearly 100%, this microfluidic device also improved production conditions by successfully separating dead cells and cell debris and increasing cell viability within the bioreactor.<\/jats:p>","DOI":"10.3390\/bioengineering10060656","type":"journal-article","created":{"date-parts":[[2023,5,28]],"date-time":"2023-05-28T15:30:47Z","timestamp":1685287847000},"page":"656","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Establishment of a Perfusion Process with Antibody-Producing CHO Cells Using a 3D-Printed Microfluidic Spiral Separator with Web-Based Flow Control"],"prefix":"10.3390","volume":"10","author":[{"given":"Jana","family":"Schellenberg","sequence":"first","affiliation":[{"name":"Institute of Technical Chemistry, Leibniz University Hannover, Callinstra\u00dfe 5, 30167 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Dehne","sequence":"additional","affiliation":[{"name":"Institute of Technical Chemistry, Leibniz University Hannover, Callinstra\u00dfe 5, 30167 Hannover, Germany"},{"name":"Institute of Physics, University of Augsburg, Universit\u00e4tsstr. 1, 86159 Augsburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferdinand","family":"Lange","sequence":"additional","affiliation":[{"name":"Institute of Technical Chemistry, Leibniz University Hannover, Callinstra\u00dfe 5, 30167 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Scheper","sequence":"additional","affiliation":[{"name":"Institute of Technical Chemistry, Leibniz University Hannover, Callinstra\u00dfe 5, 30167 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00f6rte","family":"Solle","sequence":"additional","affiliation":[{"name":"Institute of Technical Chemistry, Leibniz University Hannover, Callinstra\u00dfe 5, 30167 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7008-1673","authenticated-orcid":false,"given":"Janina","family":"Bahnemann","sequence":"additional","affiliation":[{"name":"Institute of Physics, University of Augsburg, Universit\u00e4tsstr. 1, 86159 Augsburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,28]]},"reference":[{"key":"ref_1","unstructured":"Moyle, D. 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