{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:32:15Z","timestamp":1791347535468,"version":"4.3.1"},"reference-count":43,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T00:00:00Z","timestamp":1691366400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"TRISH (Translational Research Institute for Space Health)","award":["T0602"],"award-info":[{"award-number":["T0602"]}]},{"name":"TRISH (Translational Research Institute for Space Health)","award":["PN-III-P2-2.1-PED-2019-5409"],"award-info":[{"award-number":["PN-III-P2-2.1-PED-2019-5409"]}]},{"name":"Romanian Ministry of Education and Research, CCCDI\u2013UEFISCDI","award":["T0602"],"award-info":[{"award-number":["T0602"]}]},{"name":"Romanian Ministry of Education and Research, CCCDI\u2013UEFISCDI","award":["PN-III-P2-2.1-PED-2019-5409"],"award-info":[{"award-number":["PN-III-P2-2.1-PED-2019-5409"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>This technical paper introduces a novel organ preservation system based on isochoric (constant volume) supercooling. The system is designed to enhance the stability of the metastable supercooling state, offering potential long-term preservation of large biological organs at subfreezing temperatures without the need for cryoprotectant additives. Detailed technical designs and usage protocols are provided for researchers interested in exploring this field. The paper also presents a control system based on the thermodynamics of isochoric freezing, utilizing pressure monitoring for process control. Sham experiments were performed using whole pig liver sourced from a local food supplier to evaluate the system\u2019s ability to sustain supercooling without ice nucleation for extended periods. The results demonstrated sustained supercooling without ice nucleation in pig liver tissue for 24 and 48 h. These findings suggest the potential of this technology for large-volume, cryoprotectant-free organ preservation with real-time control over the preservation process. The simplicity of the isochoric supercooling device and the design details provided in the paper are expected to serve as encouragement for other researchers in the field to pursue further research on isochoric supercooling. However, final evidence that these preserved organs can be successfully transplanted is still lacking.<\/jats:p>","DOI":"10.3390\/bioengineering10080934","type":"journal-article","created":{"date-parts":[[2023,8,7]],"date-time":"2023-08-07T06:38:48Z","timestamp":1691390328000},"page":"934","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Isochoric Supercooling Organ Preservation System"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4843-0619","authenticated-orcid":false,"given":"Gabriel","family":"N\u0103stase","sequence":"first","affiliation":[{"name":"Department of Building Services, Faculty of Civil Engineering, Transilvania University of Brasov, 500152 Brasov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florin","family":"Botea","sequence":"additional","affiliation":[{"name":"Center of Excellence in Translational Medicine CEMT, \u201cDan Setlacec\u201d Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, 022328 Bucharest, Romania"},{"name":"Department of Medical-Surgical and Profilactical Disciplines, \u201cTitu Maiorescu\u201d University, 040441 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George-Andrei","family":"Be\u0219chea","sequence":"additional","affiliation":[{"name":"Department of Building Services, Faculty of Civil Engineering, Transilvania University of Brasov, 500152 Brasov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0218tefan-Ioan","family":"C\u00e2mpean","sequence":"additional","affiliation":[{"name":"Department of Building Services, Faculty of Civil Engineering, Transilvania University of Brasov, 500152 Brasov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandru","family":"Barcu","sequence":"additional","affiliation":[{"name":"Center of Excellence in Translational Medicine CEMT, \u201cDan Setlacec\u201d Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, 022328 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ion","family":"Neac\u0219u","sequence":"additional","affiliation":[{"name":"Criomec SA, 800219 Galati, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0125-7815","authenticated-orcid":false,"given":"Vlad","family":"Herlea","sequence":"additional","affiliation":[{"name":"Center of Excellence in Translational Medicine CEMT, \u201cDan Setlacec\u201d Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, 022328 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irinel","family":"Popescu","sequence":"additional","affiliation":[{"name":"Center of Excellence in Translational Medicine CEMT, \u201cDan Setlacec\u201d Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, 022328 Bucharest, Romania"},{"name":"Department of Medical-Surgical and Profilactical Disciplines, \u201cTitu Maiorescu\u201d University, 040441 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tammy T.","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of California, San Francisco, CA 94143, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2794-1543","authenticated-orcid":false,"given":"Boris","family":"Rubinsky","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandru","family":"\u0218erban","sequence":"additional","affiliation":[{"name":"Department of Thermotechnics, Engines, Thermal and Refrigeration Equipment, Faculty of Mechanical Engineering and Mechatronics, University Politehnica of Bucharest, 060042 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1159\/000499453","article-title":"New Approaches to Cryopreservation of Cells, Tissues, and Organs","volume":"46","author":"Taylor","year":"2019","journal-title":"Transfus. 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