{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T19:27:54Z","timestamp":1778182074657,"version":"3.51.4"},"reference-count":43,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T00:00:00Z","timestamp":1698883200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"AFIRM 2","award":["CF-04 W81-XWH-14-2-0004"],"award-info":[{"award-number":["CF-04 W81-XWH-14-2-0004"]}]},{"name":"AFIRM 2","award":["R01 DE026731"],"award-info":[{"award-number":["R01 DE026731"]}]},{"name":"NIH\/NIDCR\/NIBIB","award":["CF-04 W81-XWH-14-2-0004"],"award-info":[{"award-number":["CF-04 W81-XWH-14-2-0004"]}]},{"name":"NIH\/NIDCR\/NIBIB","award":["R01 DE026731"],"award-info":[{"award-number":["R01 DE026731"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Currently used methods to repair craniomaxillofacial (CMF) bone and tooth defects require a multi-staged surgical approach for bone repair followed by dental implant placement. Our previously published results demonstrated significant bioengineered bone formation using human dental pulp stem cell (hDPSC)-seeded tyrosine-derived polycarbonate scaffolds (E1001(1K)-bTCP). Here, we improved upon this approach using a modified TyroFill (E1001(1K)\/dicalcium phosphate dihydrate (DCPD)) scaffold-supported titanium dental implant model for simultaneous bone\u2013dental implant repair. TyroFill scaffolds containing an embedded titanium implant, with (n = 3 each time point) or without (n = 2 each time point) seeded hDPCs and Human Umbilical Vein Endothelial Cells (HUVECs), were cultured in vitro. Each implant was then implanted into a 10 mm full-thickness critical-sized defect prepared on a rabbit mandibulee. After 1 and 3 months, replicate constructs were harvested and analyzed using Micro-CT histological and IHC analyses. Our results showed significant new bone formation surrounding the titanium implants in cell-seeded TyroFill constructs. This study indicates the potential utility of hDPSC\/HUVEC-seeded TyroFill scaffolds for coordinated CMF bone\u2013dental implant repair.<\/jats:p>","DOI":"10.3390\/bioengineering10111277","type":"journal-article","created":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T09:16:21Z","timestamp":1698916581000},"page":"1277","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["TyroFill\u2013Titanium Implant Constructs for the Coordinated Repair of Rabbit Mandible and Tooth Defects"],"prefix":"10.3390","volume":"10","author":[{"given":"Weibo","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthodontics, Division of Craniofacial and Molecular Genetics, Tufts University School of Dental Medicine, Boston, MA 02111, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim","family":"Kohn","sequence":"additional","affiliation":[{"name":"New Jersey Center for Biomaterials, Rutgers University, Piscataway, NJ 08854, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Yelick","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, Division of Craniofacial and Molecular Genetics, Tufts University School of Dental Medicine, Boston, MA 02111, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1038\/nrendo.2014.234","article-title":"Stromal cells and stem cells in clinical bone regeneration","volume":"11","author":"Grayson","year":"2015","journal-title":"Nat. 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