{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:54:54Z","timestamp":1787320494383,"version":"build-2736575974"},"reference-count":30,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T00:00:00Z","timestamp":1699401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["21H04955"],"award-info":[{"award-number":["21H04955"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Aortic dissection (AD) is a life-threatening tear of the vascular tissue with creation of a false lumen. To explore the mechanism underlying this tissue tear, this study investigated the delamination strength of AD model rats and the histological composition of the aorta at various stages of AD development. SD rats were administrated beta-amino propionitrile for 0 (Control), 3 (Pre-dissection), and 6 (Dissection) weeks. The thoracic aorta was harvested at 10\u201311 weeks of age. The Dissection group exclusively showed AD at the ascending aorta. The delamination strength, a force that separates the aorta in the radial direction, of the descending aorta decreased significantly in the order of the Control, Pre-dissection, and Dissection groups. A quantitative histological analysis of the aortic tissue demonstrated that, compared with the Control group, the area fraction of collagen was significantly higher in the Pre-dissection and Dissection groups and that of elastin was significantly lower in the Dissection group. The area fraction of the elastin fibers between the elastic laminas (interlaminar fibers) was significantly decreased in the order of the Control, Pre-dissection, and Dissection groups. Histological changes of the aortic tissue, perhaps a reduction in interlaminar fibers mainly aligned in the radial direction, decreased delamination strength, thereby causing AD.<\/jats:p>","DOI":"10.3390\/bioengineering10111292","type":"journal-article","created":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T07:02:05Z","timestamp":1699426925000},"page":"1292","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Delamination Strength and Elastin Interlaminar Fibers Decrease with the Development of Aortic Dissection in Model Rats"],"prefix":"10.3390","volume":"10","author":[{"given":"Genki","family":"Kurihara","sequence":"first","affiliation":[{"name":"Department of Electrical and Mechanical Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya 466-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5715-6688","authenticated-orcid":false,"given":"Yoshihiro","family":"Ujihara","sequence":"additional","affiliation":[{"name":"Department of Electrical and Mechanical Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya 466-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Electrical and Mechanical Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya 466-8555, Japan"},{"name":"Center of Biomedical Physics and Information Technology, Nagoya Institute of Technology, Nagoya 466-8555, Japan"},{"name":"Department of Nanopharmaceutical Sciences, Nagoya Institute of Technology, Nagoya 466-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0922-2465","authenticated-orcid":false,"given":"Shukei","family":"Sugita","sequence":"additional","affiliation":[{"name":"Department of Electrical and Mechanical Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya 466-8555, Japan"},{"name":"Center of Biomedical Physics and Information Technology, Nagoya Institute of Technology, Nagoya 466-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1093\/ejcts\/ezw235","article-title":"The incidence and mortality of acute thoracic aortic dissection: Results from a whole nation study","volume":"50","author":"Melvinsdottir","year":"2016","journal-title":"Eur. J. Cardiothorac. Surg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1161\/CIRCOUTCOMES.114.001140","article-title":"Trends in aortic dissection hospitalizations, interventions, and outcomes among medicare beneficiaries in the United States, 2000\u20132011","volume":"7","author":"Mody","year":"2014","journal-title":"Circ. Cardiovasc. Qual. Outcomes"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.surg.2005.06.006","article-title":"Decreased expression of fibulin-5 correlates with reduced elastin in thoracic aortic dissection","volume":"138","author":"Wang","year":"2005","journal-title":"Surgery"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/1479-5876-4-29","article-title":"Gene expression in acute Stanford type A dissection: A comparative microarray study","volume":"4","author":"Modlich","year":"2006","journal-title":"J. Transl. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"I200","DOI":"10.1161\/CIRCULATIONAHA.105.000240","article-title":"Increased collagen deposition and elevated expression of connective tissue growth factor in human thoracic aortic dissection","volume":"114","author":"Wang","year":"2006","journal-title":"Circulation"},{"key":"ref_6","first-page":"751","article-title":"Alterations of elastic architecture in human aortic dissecting aneurysm","volume":"62","author":"Nakashima","year":"1990","journal-title":"Lab. Investig."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1620\/tjem.187.291","article-title":"Alteration of cross-linking amino acids of elastin in human aorta in association with dissecting aneurysm: Analysis using high performance liquid chromatography","volume":"187","author":"Watanabe","year":"1999","journal-title":"Tohoku J. Exp. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1007\/s10439-020-02489-x","article-title":"Segmental variations in the peel characteristics of the porcine thoracic aorta","volume":"48","author":"Myneni","year":"2020","journal-title":"Ann. Biomed. Eng."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s11340-020-00644-y","article-title":"Effect of glycation on interlamellar bonding of arterial elastin","volume":"61","author":"Wang","year":"2021","journal-title":"Exp. Mech."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1007\/s10237-022-01627-9","article-title":"Is location a significant parameter in the layer dependent dissection properties of the aorta?","volume":"21","year":"2022","journal-title":"Biomech. Model. Mechanobiol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Sommer, G., Gasser, T.C., Regitnig, P., Auer, M., and Holzapfel, G.A. (2008). Dissection properties of the human aortic media: An experimental study. J. Biomech. Eng., 130.","DOI":"10.1115\/1.2898733"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1007\/s10439-011-0264-y","article-title":"Dissection properties and mechanical strength of tissue components in human carotid bifurcations","volume":"39","author":"Tong","year":"2011","journal-title":"Ann. Biomed. Eng."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.jtcvs.2011.07.058","article-title":"Effect of aneurysm on the mechanical dissection properties of the human ascending thoracic aorta","volume":"143","author":"Pasta","year":"2012","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1746","DOI":"10.2353\/ajpath.2009.081141","article-title":"Alterations in aortic cellular constituents during thoracic aortic aneurysm development: Myofibroblast-mediated vascular remodeling","volume":"175","author":"Jones","year":"2009","journal-title":"Am. J. Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/0741-5214(94)90169-4","article-title":"The composition and mechanical properties of abdominal aortic aneurysms","volume":"20","author":"He","year":"1994","journal-title":"J. Vasc. Surg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3667","DOI":"10.1016\/j.jbiomech.2016.09.040","article-title":"Controlled peel testing of a model tissue for diseased aorta","volume":"49","author":"Noble","year":"2016","journal-title":"J. Biomech."},{"key":"ref_17","unstructured":"Kurihara, G., Ujihara, Y., Nakamura, M., and Sugita, S. Effect of elastin on delamination strength in the porcine aortic tunica media. J. Biorheol., in press."},{"key":"ref_18","first-page":"560","article-title":"The expression and significance of IL-6, IFN-\u03b3, SM22\u03b1, and MMP-2 in rat model of aortic dissection","volume":"21","author":"Cai","year":"2017","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Luo, B.Y., Zhou, J., Guo, D., Yang, Q., Tian, Q., Cai, D.P., Zhou, R.M., Xu, Z.Z., Wang, H.J., and Chen, S.Y. (2022). Methamphetamine induces thoracic aortic aneurysm\/dissection through C\/EBP\u03b2. Biochimica Et Biophysica Acta\u2014Mol. Basis Dis., 1868.","DOI":"10.1016\/j.bbadis.2022.166447"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1016\/j.ejvs.2020.08.032","article-title":"Blocking Interleukin-1 Beta Reduces the Evolution of Thoracic Aortic Dissection in a Rodent Model","volume":"60","author":"Guo","year":"2020","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3643","DOI":"10.21037\/jtd-21-605","article-title":"BAPN-induced rodent model of aortic dissecting aneurysm and related complications","volume":"13","author":"Chen","year":"2021","journal-title":"J. Thorac. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3070","DOI":"10.1161\/CIRCULATIONAHA.112.097097","article-title":"Neutrophil-derived matrix metalloproteinase 9 triggers acute aortic dissection","volume":"126","author":"Kurihara","year":"2012","journal-title":"Circulation"},{"key":"ref_23","first-page":"959","article-title":"Alteration of elastic architecture in the lathyritic rat aorta implies the pathogenesis of aortic dissecting aneurysm","volume":"140","author":"Nakashima","year":"1992","journal-title":"Am. J. Pathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.21037\/atm-21-4933","article-title":"Changes in aortic collagen in \u03b2-aminopropionitrile-induced acute aortic dissection","volume":"9","author":"Zhou","year":"2021","journal-title":"Ann. Transl. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s10237-016-0851-9","article-title":"Multiphoton microscopy observations of 3D elastin and collagen fiber microstructure changes during pressurization in aortic media","volume":"16","author":"Sugita","year":"2017","journal-title":"Biomech. Model. Mechanobiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s10237-019-01201-w","article-title":"Three-dimensional analysis of the thoracic aorta microscopic deformation during intraluminal pressurization","volume":"19","author":"Sugita","year":"2020","journal-title":"Biomech. Model. Mechanobiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.5551\/jat1973.14.6_1225","article-title":"Effect of vitamin B6 on dissecting aneurysm of rats fed \u03b2-aminopropionitrile (Part 1)","volume":"14","author":"Kono","year":"1987","journal-title":"J. Jpn. Atheroscler. Soc."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1073\/pnas.61.2.708","article-title":"The cross-linking of collagen and elastin: Enzymatic conversion of lysine in peptide linkage to alpha-aminoadipic-delta-semialdehyde (allysine) by an extract from bone","volume":"61","author":"Pinnell","year":"1968","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.matbio.2007.10.008","article-title":"The three-dimensional micro- and nanostructure of the aortic medial lamellar unit measured using 3D confocal and electron microscopy imaging","volume":"27","author":"Murthy","year":"2008","journal-title":"Matrix Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"eaaz1173","DOI":"10.1126\/sciadv.aaz1173","article-title":"Avalanches and power law behavior in aortic dissection propagation","volume":"6","author":"Yu","year":"2020","journal-title":"Sci. Adv."}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/10\/11\/1292\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:19:14Z","timestamp":1760131154000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/10\/11\/1292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,8]]},"references-count":30,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,11]]}},"alternative-id":["bioengineering10111292"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering10111292","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,8]]}}}