{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T03:22:14Z","timestamp":1783999334687,"version":"3.55.0"},"reference-count":82,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T00:00:00Z","timestamp":1699833600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NSERC Discovery","award":["RGPIN-2019-05731"],"award-info":[{"award-number":["RGPIN-2019-05731"]}]},{"name":"NSERC Discovery","award":["PJT 180556"],"award-info":[{"award-number":["PJT 180556"]}]},{"name":"NSERC Discovery","award":["RGPIN-2022-03898"],"award-info":[{"award-number":["RGPIN-2022-03898"]}]},{"name":"Li Ka Shing Foundation","award":["RGPIN-2019-05731"],"award-info":[{"award-number":["RGPIN-2019-05731"]}]},{"name":"Li Ka Shing Foundation","award":["PJT 180556"],"award-info":[{"award-number":["PJT 180556"]}]},{"name":"Li Ka Shing Foundation","award":["RGPIN-2022-03898"],"award-info":[{"award-number":["RGPIN-2022-03898"]}]},{"name":"Canadian Institute of Health Research Project","award":["RGPIN-2019-05731"],"award-info":[{"award-number":["RGPIN-2019-05731"]}]},{"name":"Canadian Institute of Health Research Project","award":["PJT 180556"],"award-info":[{"award-number":["PJT 180556"]}]},{"name":"Canadian Institute of Health Research Project","award":["RGPIN-2022-03898"],"award-info":[{"award-number":["RGPIN-2022-03898"]}]},{"name":"NSERC Discovery","award":["RGPIN-2019-05731"],"award-info":[{"award-number":["RGPIN-2019-05731"]}]},{"name":"NSERC Discovery","award":["PJT 180556"],"award-info":[{"award-number":["PJT 180556"]}]},{"name":"NSERC Discovery","award":["RGPIN-2022-03898"],"award-info":[{"award-number":["RGPIN-2022-03898"]}]},{"name":"CIHR (Canada Graduate Scholarship)","award":["RGPIN-2019-05731"],"award-info":[{"award-number":["RGPIN-2019-05731"]}]},{"name":"CIHR (Canada Graduate Scholarship)","award":["PJT 180556"],"award-info":[{"award-number":["PJT 180556"]}]},{"name":"CIHR (Canada Graduate Scholarship)","award":["RGPIN-2022-03898"],"award-info":[{"award-number":["RGPIN-2022-03898"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Neural stem cell (NSC)-based therapies are at the forefront of regenerative medicine strategies for various neural defects and injuries such as stroke, traumatic brain injury, and spinal cord injury. For several clinical applications, NSC therapies require biocompatible scaffolds to support cell survival and to direct differentiation. Here, we investigate decellularized plant tissue as a novel scaffold for three-dimensional (3D), in vitro culture of NSCs. Plant cellulose scaffolds were shown to support the attachment and proliferation of adult rat hippocampal neural stem cells (NSCs). Further, NSCs differentiated on the cellulose scaffold had significant increases in their expression of neuron-specific beta-III tubulin and glial fibrillary acidic protein compared to 2D culture on a polystyrene plate, indicating that the scaffold may enhance the differentiation of NSCs towards astrocytic and neuronal lineages. Our findings suggest that plant-derived cellulose scaffolds have the potential to be used in neural tissue engineering and can be harnessed to direct the differentiation of NSCs.<\/jats:p>","DOI":"10.3390\/bioengineering10111309","type":"journal-article","created":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T02:02:42Z","timestamp":1699840962000},"page":"1309","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Plant Cellulose as a Substrate for 3D Neural Stem Cell Culture"],"prefix":"10.3390","volume":"10","author":[{"given":"Lauren J.","family":"Couvrette","sequence":"first","affiliation":[{"name":"Department of Biology, University of Ottawa, Gendron Hall, 30 Marie Curie, Ottawa, ON K1N 5N5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krystal L. 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