{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:23:44Z","timestamp":1789179824997,"version":"build-2803163510"},"reference-count":32,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T00:00:00Z","timestamp":1704412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Career Advancement Award from Research to Prevent Blindness (RPB)","award":["NIH P30 EY026877"],"award-info":[{"award-number":["NIH P30 EY026877"]}]},{"name":"National Eye Institute","award":["NIH P30 EY026877"],"award-info":[{"award-number":["NIH P30 EY026877"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>The corneal endothelium, comprising densely packed corneal endothelial cells (CECs) adhering to Descemet\u2019s membrane (DM), plays a critical role in maintaining corneal transparency by regulating water and ion movement. CECs have limited regenerative capacity within the body, and globally, there is a shortage of donor corneas to replace damaged corneal endothelia. The development of a carrier for cultured CECs may address this worldwide clinical need. In this study we successfully manufactured a gelatin nanofiber membrane (gelNF membrane) using electrospinning, followed by crosslinking with glutaraldehyde (GA). The fabricated gelNF membrane exhibited approximately 80% transparency compared with glass and maintained a thickness of 20 \u00b5m. The gelNF membrane demonstrated desirable permeability and degradability for a Descemet\u2019s membrane analog. Importantly, CECs cultured on the gelNF membrane at high densities showed no cytotoxic effects, and the expression of key CEC functional biomarkers was verified. To assess the potential of this gelNF membrane as a carrier for cultured CEC transplantation, we used it to conduct Descemet\u2019s membrane endothelial keratoplasty (DMEK) on rabbit eyes. The outcomes suggest this gelNF membrane holds promise as a suitable carrier for cultured CEC transplantation, offering advantages in terms of transparency, permeability, and sufficient mechanical properties required for successful transplantation.<\/jats:p>","DOI":"10.3390\/bioengineering11010054","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T07:38:31Z","timestamp":1704440311000},"page":"54","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Electrospun Nanofiber Membrane for Cultured Corneal Endothelial Cell Transplantation"],"prefix":"10.3390","volume":"11","author":[{"given":"Euisun","family":"Song","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Spencer Center for Vision Research, Byers Eye Institute at Stanford University, Palo Alto, CA 94304, USA"},{"name":"VA Palo Alto Health Care System, Palo Alto, CA 94304, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen M.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 120-749, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathew S.","family":"Margolis","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Spencer Center for Vision Research, Byers Eye Institute at Stanford University, Palo Alto, CA 94304, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thitima","family":"Wungcharoen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Spencer Center for Vision Research, Byers Eye Institute at Stanford University, Palo Alto, CA 94304, USA"},{"name":"Ophthalmology, MedPark Hospital, Bangkok 10110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won-Gun","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 120-749, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Myung","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Spencer Center for Vision Research, Byers Eye Institute at Stanford University, Palo Alto, CA 94304, USA"},{"name":"VA Palo Alto Health Care System, Palo Alto, CA 94304, USA"},{"name":"Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1038\/sj.eye.6700559","article-title":"Biology of the corneal endothelium in health and disease","volume":"17","author":"Bourne","year":"2003","journal-title":"Eye"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"E239","DOI":"10.1097\/OPX.0b013e3181d39464","article-title":"Dynamic regulation of barrier integrity of the corneal endothelium","volume":"87","author":"Srinivas","year":"2010","journal-title":"Optom. 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