{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T03:23:14Z","timestamp":1793157794756,"version":"4.5.0"},"reference-count":32,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T00:00:00Z","timestamp":1708300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Apollo Medical Optics, Ltd.","award":["XMRPG3L0841"],"award-info":[{"award-number":["XMRPG3L0841"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Vitiligo, a psychologically distressing pigmentary disorder characterized by white depigmented patches due to melanocyte loss, necessitates non-invasive tools for early detection and treatment response monitoring. High-cellular-resolution full-field optical coherence tomography (CRFF-OCT) is emerging in pigmentary disorder assessment, but its applicability in vitiligo repigmentation after tissue grafting remains unexplored. To investigate the feasibility of CRFF-OCT for evaluating vitiligo lesions following tissue grafting, our investigation involved ten vitiligo patients who underwent suction blister epidermal grafting and laser ablation at a tertiary center between 2021 and 2022. Over a six-month period, clinical features, dermoscopy, and photography data were recorded. Utilizing CRFF-OCT along with artificial intelligence (AI) applications, repigmentation features were captured and analyzed. The CRFF-OCT analysis revealed a distinct dark band in vitiligo lesion skin, indicating melanin loss. Grafted areas exhibited melanocytes with dendrites around the epidermal-dermal junction and hair follicles. CRFF-OCT demonstrated its efficacy in the early detection of melanocyte recovery and accurate melanin quantification. This study introduces CRFF-OCT as a real-time, non-invasive, and in vivo evaluation tool for assessing vitiligo repigmentation, offering valuable insights into pigmentary disorders and treatment responses.<\/jats:p>","DOI":"10.3390\/bioengineering11020196","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T05:39:56Z","timestamp":1708321196000},"page":"196","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Feasibility of High-Cellular-Resolution Full-Field, Artificial-Intelligence-Assisted, Real-Time Optical Coherence Tomography in the Evaluation of Vitiligo: A Prospective Longitudinal Follow-Up Study"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2122-5471","authenticated-orcid":false,"given":"Lai-Ying","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Dermatology, Chang Gung Memorial Hospital, Linkou Main Branch, Taoyuan 333423, Taiwan"},{"name":"Department of Dermatology and Aesthetic Medicine Center, Jen-Ai Hospital, Taichung 412224, Taiwan"},{"name":"College of Medicine, Chang Gung University, Taoyuan 333323, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Apollo Medical Optics, Ltd., Taipei 114, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1270-4198","authenticated-orcid":false,"given":"I-Ling","family":"Chen","sequence":"additional","affiliation":[{"name":"Apollo Medical Optics, Ltd., Taipei 114, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chang","family":"Shih","sequence":"additional","affiliation":[{"name":"Apollo Medical Optics, Ltd., Taipei 114, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0910-8558","authenticated-orcid":false,"given":"Rosalie Tzu-Li","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Chang Gung Memorial Hospital, Linkou Main Branch, Taoyuan 333423, Taiwan"},{"name":"College of Medicine, Chang Gung University, Taoyuan 333323, Taiwan"},{"name":"Vitiligo Clinic and Pigment Research Center, Chang Gung Memorial Hospital, Linkou District, New Taipei 33305, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Jing","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Chang Gung Memorial Hospital, Linkou Main Branch, Taoyuan 333423, Taiwan"},{"name":"Vitiligo Clinic and Pigment Research Center, Chang Gung Memorial Hospital, Linkou District, New Taipei 33305, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chau Yee","family":"Ng","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Chang Gung Memorial Hospital, Linkou Main Branch, Taoyuan 333423, Taiwan"},{"name":"Department of Dermatology and Aesthetic Medicine Center, Jen-Ai Hospital, Taichung 412224, Taiwan"},{"name":"College of Medicine, Chang Gung University, Taoyuan 333323, Taiwan"},{"name":"Apollo Medical Optics, Ltd., Taipei 114, Taiwan"},{"name":"Vitiligo Clinic and Pigment Research Center, Chang Gung Memorial Hospital, Linkou District, New Taipei 33305, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1111\/j.1468-3083.2012.04505.x","article-title":"Assessment methods for the evaluation of vitiligo","volume":"26","author":"Alghamdi","year":"2012","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1159\/000506103","article-title":"Vitiligo: A Review","volume":"236","author":"Bergqvist","year":"2020","journal-title":"Dermatology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1001\/archderm.140.6.677","article-title":"Parametric modeling of narrowband UV-B phototherapy for vitiligo using a novel quantitative tool: The Vitiligo Area Scoring Index","volume":"140","author":"Hamzavi","year":"2004","journal-title":"Arch. Dermatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1016\/j.jaad.2019.10.055","article-title":"Standardizing serial photography for assessing and monitoring vitiligo: A core set of international recommendations for essential clinical and technical specifications","volume":"83","author":"Hamzavi","year":"2020","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"505","DOI":"10.4103\/0378-6323.144144","article-title":"The utility of dermoscopy in the diagnosis of evolving lesions of vitiligo","volume":"80","author":"Thatte","year":"2014","journal-title":"Indian. J. Dermatol. Venereol. Leprol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Hou, Y., Wei, Z., Jiang, Q., Chen, H., Chen, L., and Wu, J. (2023). In-depth study of Wood\u2019s lamp examination combined with reflective confocal laser scanning microscopy for the guidance of vitiligo staging and treatment. J. Cosmet. Dermatol.","DOI":"10.1111\/jocd.16145"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1111\/jdv.16226","article-title":"Ultraviolet photography in vitiligo: Image quality, validity and reliability","volume":"34","author":"Uitentuis","year":"2020","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1111\/j.1600-0781.2008.00383.x","article-title":"The perilesional skin in vitiligo: A colorimetric in vivo study of 25 patients","volume":"24","author":"Brazzelli","year":"2008","journal-title":"Photodermatol. Photoimmunol. Photomed."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"429","DOI":"10.4103\/0019-5154.119948","article-title":"Role of in vivo reflectance confocal microscopy in determining stability in vitiligo: A preliminary study","volume":"58","author":"Li","year":"2013","journal-title":"Indian. J. Dermatol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1111\/srt.12638","article-title":"The role of in vivo reflectance confocal microscopy in assessing the stability of vitiligo vulgaris prior to cellular grafting","volume":"25","author":"Chuah","year":"2019","journal-title":"Skin. Res. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1111\/j.1600-0846.2011.00521.x","article-title":"In vivo reflectance confocal microscopy imaging of vitiligo, nevus depigmentosus and nevus anemicus","volume":"17","author":"Lai","year":"2011","journal-title":"Skin. Res. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1111\/j.1468-3083.2007.02275.x","article-title":"Preliminary evaluation of vitiligo using in vivo reflectance confocal microscopy","volume":"21","author":"Ardigo","year":"2007","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_13","first-page":"7366","article-title":"Vitiligo management: Combination of surgical treatment and phototherapy under reflectance confocal microscopy monitoring","volume":"24","author":"Cortelazzi","year":"2020","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1117\/1.JBO.23.4.040901","article-title":"Advances in optical coherence tomography in dermatology-a review","volume":"23","author":"Olsen","year":"2018","journal-title":"J. Biomed. Opt."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Chen, I.L., Wang, Y.J., Chang, C.C., Wu, Y.H., Lu, C.W., Shen, J.W., Huang, L., Lin, B.S., and Chiang, H.M. (2021). Computer-Aided Detection (CADe) System with Optical Coherent Tomography for Melanin Morphology Quantification in Melasma Patients. Diagnostics, 11.","DOI":"10.3390\/diagnostics11081498"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/s10103-018-2696-1","article-title":"Optical coherence tomography imaging of melanoma skin cancer","volume":"34","author":"Bittar","year":"2019","journal-title":"Lasers Med. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1002\/lsm.23386","article-title":"Reflectance Confocal Microscopy, Optical Coherence Tomography, and Multiphoton Microscopy in Inflammatory Skin Disease Diagnosis","volume":"53","author":"Csuka","year":"2021","journal-title":"Lasers Surg. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1002\/lsm.23219","article-title":"Use of Optical Coherence Tomography (OCT) in Aesthetic Skin Assessment-A Short Review","volume":"52","author":"Kislevitz","year":"2020","journal-title":"Lasers Surg. Med."},{"key":"ref_19","first-page":"187","article-title":"Artificial Intelligence and Optical Coherence Tomography Imaging","volume":"8","author":"Kapoor","year":"2019","journal-title":"Asia Pac. J. Ophthalmol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1080\/08820538.2021.1901123","article-title":"Artificial Intelligence (AI) and Retinal Optical Coherence Tomography (OCT)","volume":"36","author":"Dahrouj","year":"2021","journal-title":"Semin. Ophthalmol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1001\/jamaophthalmol.2023.1829","article-title":"Optical Coherence Tomography Angiography, Artificial Intelligence, and the Missing Capillaries","volume":"141","author":"Kashani","year":"2023","journal-title":"JAMA Ophthalmol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Tsai, M.R., Ho, T.S., Wu, Y.H., and Lu, C.W. (2021). In Vivo dual-mode full-field optical coherence tomography for differentiation of types of melanocytic nevi. J. Biomed. Opt., 26.","DOI":"10.1117\/1.JBO.26.2.020501"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1159\/000514686","article-title":"Application of Cellular Resolution Full-Field Optical Coherence Tomography in vivo for the Diagnosis of Skin Tumours and Inflammatory Skin Diseases: A Pilot Study","volume":"238","author":"Wang","year":"2022","journal-title":"Dermatology"},{"key":"ref_24","unstructured":"Heckbert, P.S. (1994). Graphics Gems, Academic Press."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Frangi, A.F., Niessen, W.J., Vincken, K.L., and Viergever, M.A. (1998, January 11\u201313). Multiscale vessel enhancement filtering. Proceedings of the International Conference on Medical Image Computing and Computer-Assisted Intervention, Cambridge, MA, USA.","DOI":"10.1007\/BFb0056195"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1111\/jdv.12954","article-title":"High-definition optical coherence tomography algorithm for the discrimination of actinic keratosis from normal skin and from squamous cell carcinoma","volume":"29","author":"Boone","year":"2015","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.1111\/jdv.12935","article-title":"Assessment of a scoring system for Basal Cell Carcinoma with multi-beam optical coherence tomography","volume":"29","author":"Wahrlich","year":"2015","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1111\/jdv.14369","article-title":"Acne: Morphologic and vascular study of lesions and surrounding skin by means of optical coherence tomography","volume":"31","author":"Manfredini","year":"2017","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1159\/000449230","article-title":"Vascular Features of Nail Psoriasis Using Dynamic Optical Coherence Tomography","volume":"2","author":"Aldahan","year":"2017","journal-title":"Skin Appendage Disord."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s00403-014-1492-4","article-title":"Differentiating allergic and irritant contact dermatitis by high-definition optical coherence tomography: A pilot study","volume":"307","author":"Boone","year":"2015","journal-title":"Arch. Dermatol. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e97","DOI":"10.1016\/j.jaad.2010.02.010","article-title":"A noninvasive technique, reflectance confocal microscopy, for the characterization of melanocyte loss in untreated and treated vitiligo lesions","volume":"63","author":"Kang","year":"2010","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1111\/j.1365-4632.2010.04841.x","article-title":"In vivo reflectance confocal microscopy for the differential diagnosis between vitiligo and nevus depigmentosus","volume":"50","author":"Pan","year":"2011","journal-title":"Int. J. Dermatol."}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/11\/2\/196\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:02:12Z","timestamp":1760104932000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/11\/2\/196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,19]]},"references-count":32,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["bioengineering11020196"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering11020196","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,19]]}}}