{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:59:57Z","timestamp":1760144397991,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,4,20]],"date-time":"2024-04-20T00:00:00Z","timestamp":1713571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["19H03741"],"award-info":[{"award-number":["19H03741"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>This study investigated six-month outcomes of first models of ascending aortic replacement. The molds used to produce the Biotube were implanted subcutaneously in goats. After 2\u20133 months, the molds were explanted to obtain the Biotubes (inner diameter, 12 mm; wall thickness, 1.5 mm). Next, we performed ascending aortic replacement using the Biotube in five allogenic goats. At 6 months, the animals underwent computed tomography (CT) and histologic evaluation. As a comparison, we performed similar surgeries using glutaraldehyde-fixed autologous pericardial rolls or pig-derived heterogenous Biotubes. At 6 months, CT revealed no aneurysmalization of the Biotube or pseudoaneurysm formation. The histologic evaluation showed development of endothelial cells, smooth muscle cells, and elastic fibers along the Biotube. In the autologous pericardium group, there was no evidence of new cell development, but there was calcification. The histologic changes observed in the heterologous Biotube group were similar to those in the allogenic Biotube group. However, there was inflammatory cell infiltration in some heterologous Biotubes. Based on the above, we could successfully create the world\u2019s first Biotube-based ascending aortic replacement models. The present results indicate that the Biotube may serve as a scaffold for aortic tissue regeneration.<\/jats:p>","DOI":"10.3390\/bioengineering11040405","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T07:44:09Z","timestamp":1713771849000},"page":"405","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Breaking the Limit of Cardiovascular Regenerative Medicine: Successful 6-Month Goat Implant in World\u2019s First Ascending Aortic Replacement Using Biotube Blood Vessels"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8608-7888","authenticated-orcid":false,"given":"Kazuki","family":"Mori","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Surgery, Oita University, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadashi","family":"Umeno","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Oita University, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1631-8497","authenticated-orcid":false,"given":"Takayuki","family":"Kawashima","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Oita University, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Wada","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Oita University, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuro","family":"Genda","sequence":"additional","affiliation":[{"name":"Department of Clinical Engineering, Oita University Hospital, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanagi","family":"Arakura","sequence":"additional","affiliation":[{"name":"Department of Clinical Engineering, Oita University Hospital, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshifumi","family":"Oda","sequence":"additional","affiliation":[{"name":"Department of Clinical Engineering, Oita University Hospital, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Mizoguchi","sequence":"additional","affiliation":[{"name":"Department of Clinical Engineering, Oita University Hospital, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryosuke","family":"Iwai","sequence":"additional","affiliation":[{"name":"Institute of Frontier Science and Technology, Okayama University of Science, Okayama 700-0005, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5304-9703","authenticated-orcid":false,"given":"Tsutomu","family":"Tajikawa","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Faculty of Engineering Science, Kansai University, Osaka 564-8680, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhide","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Osaka Laboratory, Biotube Co., Ltd., Osaka 565-0842, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4972-0717","authenticated-orcid":false,"given":"Shinji","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Oita University, Oita 879-5593, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1093\/icvts\/ivv024","article-title":"Prosthetic vascular graft infection through a median sternotomy: A multicentre review","volume":"20","author":"Oda","year":"2015","journal-title":"Interact. 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