{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:43:27Z","timestamp":1790109807099,"version":"4.0.1"},"reference-count":63,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,4,28]],"date-time":"2024-04-28T00:00:00Z","timestamp":1714262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"LifeLab Program of the \u2018Consorzio per la Ricerca Sanitaria (CORIS) of the Veneto Region, Italy","award":["DGR1017, 17 July 2018"],"award-info":[{"award-number":["DGR1017, 17 July 2018"]}]},{"name":"LifeLab Program of the \u2018Consorzio per la Ricerca Sanitaria (CORIS) of the Veneto Region, Italy","award":["Progetto BIRD 238994-2023"],"award-info":[{"award-number":["Progetto BIRD 238994-2023"]}]},{"name":"Department of Industrial Engineering (University of Padova)","award":["DGR1017, 17 July 2018"],"award-info":[{"award-number":["DGR1017, 17 July 2018"]}]},{"name":"Department of Industrial Engineering (University of Padova)","award":["Progetto BIRD 238994-2023"],"award-info":[{"award-number":["Progetto BIRD 238994-2023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>The production of biomedical devices able to appropriately interact with the biological environment is still a great challenge. Synthetic materials are often employed, but they fail to replicate the biological and functional properties of native tissues, leading to a variety of adverse effects. Several commercial products are based on chemically treated xenogeneic tissues: their principal drawback is due to weak mechanical stability and low durability. Recently, decellularization has been proposed to bypass the drawbacks of both synthetic and biological materials. Acellular materials can integrate with host tissues avoiding\/mitigating any foreign body response, but they often lack sufficient patency and impermeability. The present paper investigates an innovative approach to the realization of hybrid materials that combine decellularized bovine pericardium with polycarbonate urethanes. These hybrid materials benefit from the superior biocompatibility of the biological tissue and the mechanical properties of the synthetic polymers. They were assessed from physicochemical, structural, mechanical, and biological points of view; their ability to promote cell growth was also investigated. The decellularized pericardium and the polymer appeared to well adhere to each other, and the two sides were distinguishable. The maximum elongation of hybrid materials was mainly affected by the pericardium, which allows for lower elongation than the polymer; this latter, in turn, influenced the maximum strength achieved. The results confirmed the promising features of hybrid materials for the production of vascular grafts able to be repopulated by circulating cells, thus, improving blood compatibility.<\/jats:p>","DOI":"10.3390\/bioengineering11050436","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T09:50:07Z","timestamp":1714470607000},"page":"436","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Hybrid Materials for Vascular Applications: A Preliminary In Vitro Assessment"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4906-5257","authenticated-orcid":false,"given":"Martina","family":"Todesco","sequence":"first","affiliation":[{"name":"Department of Civil, Environmental and Architectural Engineering, University of Padua, Via Marzolo 9, 35131 Padua, Italy"},{"name":"L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Giustiniani 2, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8212-1484","authenticated-orcid":false,"given":"Martina","family":"Casarin","sequence":"additional","affiliation":[{"name":"L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Giustiniani 2, 35128 Padova, Italy"},{"name":"Department of Surgery, Oncology and Gastroenterology, Giustiniani 2, 35128 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Sandrin","sequence":"additional","affiliation":[{"name":"L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Giustiniani 2, 35128 Padova, Italy"},{"name":"Department of Physics and Astronomy \u2018G. Galilei\u2019, University of Padova, Via Marzolo 8, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3291-8567","authenticated-orcid":false,"given":"Laura","family":"Astolfi","sequence":"additional","affiliation":[{"name":"L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Giustiniani 2, 35128 Padova, Italy"},{"name":"Department of Neurosciences, University of Padua, Via Giustiniani, 2, 35128 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0243-1464","authenticated-orcid":false,"given":"Filippo","family":"Romanato","sequence":"additional","affiliation":[{"name":"L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Giustiniani 2, 35128 Padova, Italy"},{"name":"Department of Physics and Astronomy \u2018G. Galilei\u2019, University of Padova, Via Marzolo 8, 35131 Padova, Italy"},{"name":"CNR-INFM TASC IOM National Laboratory, S.S. 14 Km 163.5, Basovizza, 34012 Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Germana","family":"Giuggioli","sequence":"additional","affiliation":[{"name":"Department of Prevention Veterinary Services, ULSS 3 Serenissima, P.le S.L Giustiniani 11\/D Mestre, 30174 Venice, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Conte","sequence":"additional","affiliation":[{"name":"Department of Prevention Veterinary Services, ULSS 3 Serenissima, P.le S.L Giustiniani 11\/D Mestre, 30174 Venice, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6261-699X","authenticated-orcid":false,"given":"Gino","family":"Gerosa","sequence":"additional","affiliation":[{"name":"L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, Via Giustiniani 2, 35128 Padova, Italy"},{"name":"Department of Cardiac, Thoracic Vascular Sciences and Public Health, University of Padova, Via Giustiniani 2, 35128 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3844-9842","authenticated-orcid":false,"given":"Chiara Giulia","family":"Fontanella","sequence":"additional","affiliation":[{"name":"Department of Industrial Engineering, University of Padua, Via Marzolo 9, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4296-3601","authenticated-orcid":false,"given":"Andrea","family":"Bagno","sequence":"additional","affiliation":[{"name":"L.i.f.e.L.a.b. 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Encyclopedia of Materials: Plastics and Polymers, Elsevier."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"100686","DOI":"10.1016\/j.pmatsci.2020.100686","article-title":"Advanced Hybrid Nanomaterials for Biomedical Applications","volume":"114","author":"Park","year":"2020","journal-title":"Prog. Mater. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"118179","DOI":"10.1016\/j.carbpol.2021.118179","article-title":"Fabrication and Applications of Bioactive Chitosan-Based Organic-Inorganic Hybrid Materials: A Review","volume":"267","author":"Liu","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3631","DOI":"10.1021\/acs.nanolett.3c04876","article-title":"Emergence, Pattern, and Frequency of Spontaneous Waves in Spreading Epithelial Monolayers","volume":"24","author":"Yin","year":"2024","journal-title":"Nano Lett."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1007\/s10965-014-0410-y","article-title":"Collagen Coated Electrospun Polycaprolactone (PCL) with Titanium Dioxide (TiO2) from an Environmentally Benign Solvent: Preliminary Physico-Chemical Studies for Skin Substitute","volume":"21","author":"Ghosal","year":"2014","journal-title":"J. Polym. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2933","DOI":"10.1089\/ten.2007.0052","article-title":"Fabrication of an Optimal Urethral Graft Using Collagen-Sponge Tubes Reinforced with Copoly(L-Lactide\/\u03b5-Caprolactone) Fabric","volume":"13","author":"Kanatani","year":"2007","journal-title":"Tissue Eng."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3969","DOI":"10.1016\/j.biomaterials.2011.02.012","article-title":"A Collagen-Poly(Lactic Acid-Co-\u025b-Caprolactone) Hybrid Scaffold for Bladder Tissue Regeneration","volume":"32","author":"Engelhardt","year":"2011","journal-title":"Biomaterials"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1089\/ten.tea.2008.0194","article-title":"A Poly(Lactic Acid-Co-Caprolactone)\u2013Collagen Hybrid for Tissue Engineering Applications","volume":"15","author":"Ananta","year":"2009","journal-title":"Tissue Eng. Part A"},{"key":"ref_11","first-page":"177","article-title":"Antithrombotic Stent Coatings: Hirudin\/Iloprost Combination","volume":"3","author":"Alt","year":"1998","journal-title":"Semin. Interv. Cardiol. SIIC"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1002\/jbm.b.31650","article-title":"Polymer Coating of Porcine Decellularized and Cross-Linked Aortic Grafts","volume":"94B","author":"Heidenhain","year":"2010","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1016\/j.athoracsur.2004.03.106","article-title":"Biomatrix\/Polymer Composite Material for Heart Valve Tissue Engineering","volume":"78","author":"Stamm","year":"2004","journal-title":"Ann. Thorac. Surg."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1111\/j.1525-1594.2004.00007.x","article-title":"Mechanical and Structural Properties of a Novel Hybrid Heart Valve Scaffold for Tissue Engineering","volume":"28","author":"Grabow","year":"2004","journal-title":"Artif. Organs"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1089\/ten.teb.2015.0100","article-title":"The Tissue-Engineered Vascular Graft\u2014Past, Present, and Future","volume":"22","author":"MacNeil","year":"2016","journal-title":"Tissue Eng. Part B Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2362","DOI":"10.1016\/j.jhsa.2012.07.028","article-title":"A Systematic Review of the Outcomes of Distal Upper Extremity Bypass Surgery with Arterial and Venous Conduits","volume":"37","author":"Masden","year":"2012","journal-title":"J. Hand Surg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.1016\/j.jacc.2004.08.064","article-title":"Long-Term Patency of Saphenous Vein and Left Internal Mammary Artery Grafts after Coronary Artery Bypass Surgery","volume":"44","author":"Goldman","year":"2004","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7350","DOI":"10.1021\/acs.nanolett.3c01712","article-title":"New Mechanical Markers for Tracking the Progression of Myocardial Infarction","volume":"23","author":"Chang","year":"2023","journal-title":"Nano Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1097\/SLA.0b013e318288c38d","article-title":"Saphenous Vein Graft Failure After Coronary Artery Bypass Surgery: Pathophysiology, Management, and Future Directions","volume":"257","author":"Harskamp","year":"2013","journal-title":"Ann. Surg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.ejvs.2003.12.027","article-title":"Saphenous Vein Versus PTFE for Above-Knee Femoropopliteal Bypass. A Review of the Literature","volume":"27","author":"Klinkert","year":"2004","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1089\/ten.teb.2014.0123","article-title":"In Vivo Applications of Electrospun Tissue-Engineered Vascular Grafts: A Review","volume":"20","author":"Rocco","year":"2014","journal-title":"Tissue Eng.-Part B Rev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s10047-003-0232-x","article-title":"Intimal Hyperplasia and Hemodynamic Factors in Arterial Bypass and Arteriovenous Grafts: A Review","volume":"6","author":"Haruguchi","year":"2003","journal-title":"J. Artif. Organs"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/j.ejvs.2006.01.006","article-title":"The Mechanical Properties of Infrainguinal Vascular Bypass Grafts: Their Role in Influencing Patency","volume":"31","author":"Sarkar","year":"2006","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1002\/(SICI)1096-9896(200002)190:3<292::AID-PATH528>3.0.CO;2-S","article-title":"Improving Vascular Grafts: The Importance of Mechanical and Haemodynamic Properties","volume":"190","author":"Greenwald","year":"2000","journal-title":"J. Pathol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S1078-5884(05)80218-7","article-title":"Pathophysiology of Vein Graft Failure: A Review","volume":"9","author":"Davies","year":"1995","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Hibino, N., McGillicuddy, E., Matsumura, G., Ichihara, Y., Naito, Y., Breuer, C., and Shinoka, T. (2010). Late-Term Results of Tissue-Engineered Vascular Grafts in Humans. J. Thorac. Cardiovasc. Surg., 139.","DOI":"10.1016\/j.jtcvs.2009.09.057"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1007\/BF02344876","article-title":"Preparation of a Pure Autologous Biodegradable Fibrin Matrix for Tissue Engineering","volume":"38","author":"Haisch","year":"2000","journal-title":"Med. Biol. Eng. Comput."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1177\/088532829400800303","article-title":"A Solution Grade Biostable Polyurethane Elastomer: ChronoFlex\u00ae AR","volume":"8","author":"Reed","year":"1994","journal-title":"J. Biomater. Appl."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Todesco, M., Zardin, C., Iop, L., Palmosi, T., Capaldo, P., Romanato, F., Gerosa, G., and Bagno, A. (2021). Hybrid Membranes for the Production of Blood Contacting Surfaces: Physicochemical, Structural and Biomechanical Characterization. Biomater. Res., 25.","DOI":"10.1186\/s40824-021-00227-5"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"807255","DOI":"10.3389\/fcvm.2022.807255","article-title":"A Biohybrid Material with Extracellular Matrix Core and Polymeric Coating as a Cell Honing Cardiovascular Tissue Substitute","volume":"9","author":"Mudigonda","year":"2022","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1016\/j.eng.2020.05.021","article-title":"Visible Light-Induced 3D Bioprinting Technologies and Corresponding Bioink Materials for Tissue Engineering: A Review","volume":"7","author":"Zheng","year":"2021","journal-title":"Engineering"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.ymeth.2008.10.014","article-title":"Composite Scaffolds for the Engineering of Hollow Organs and Tissues","volume":"47","author":"Eberli","year":"2009","journal-title":"Methods"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1016\/j.biomaterials.2012.10.075","article-title":"A Bilayered Hybrid Microfibrous PLGA\u2013Acellular Matrix Scaffold for Hollow Organ Tissue Engineering","volume":"34","author":"Horst","year":"2013","journal-title":"Biomaterials"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1002\/(SICI)1097-4636(1999)48:5<648::AID-JBM8>3.0.CO;2-6","article-title":"Assessing the Resistance to Calcification of Polyurethane Membranes Used in the Manufacture of Ventricles for a Totally Implantable Artificial Heart","volume":"48","author":"Yang","year":"1999","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/(SICI)1097-4636(1999)48:1<13::AID-JBM4>3.0.CO;2-4","article-title":"Totally Implantable Artificial Hearts and Left Ventricular Assist Devices: Selecting Impermeable Polycarbonate Urethane to Manufacture Ventricles","volume":"48","author":"Yang","year":"1999","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1016\/j.athoracsur.2004.06.033","article-title":"Calcification of Tissue Heart Valve Substitutes: Progress Toward Understanding and Prevention","volume":"79","author":"Schoen","year":"2005","journal-title":"Ann. Thorac. Surg."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Todesco, M., Imran, S.J., Fortunato, T.M., Sandrin, D., Borile, G., Romanato, F., Casarin, M., Giuggioli, G., Conte, F., and Marchesan, M. (2022). A New Detergent for the Effective Decellularization of Bovine and Porcine Pericardia. Biomimetics, 7.","DOI":"10.3390\/biomimetics7030104"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Cassari, L., Todesco, M., Zamuner, A., Imran, S.J., Casarin, M., Sandrin, D., R\u00f3denas-Rochina, J., Gomez Ribelles, J.L., Romanato, F., and Bagno, A. (2023). Covalently Grafted Peptides to Decellularized Pericardium: Modulation of Surface Density. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24032932"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1111\/aor.13065","article-title":"Native Bovine and Porcine Pericardia Respond to Load with Additive Recruitment of Collagen Fibers: Additive Recruitment of Collagen Fibers","volume":"42","author":"Bagno","year":"2018","journal-title":"Artif. Organs"},{"key":"ref_40","unstructured":"Todesco, M., Merigliano, G., Candela, V., Iop, L., Palmosi, T., Gerosa, G., and Bagno, A. (2020, January 10\u201312). Hybrid Membranes for Blood-Contacting Surfaces: Preliminary Characterization. Proceedings of the Seventh National Congress of Bioengineering, Trieste, Italy."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.actbio.2017.11.035","article-title":"A Sterilization Method for Decellularized Xenogeneic Cardiovascular Scaffolds","volume":"67","author":"Fidalgo","year":"2018","journal-title":"Acta Biomater."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1021\/ja0400685","article-title":"A Quantitative Reconstruction of the Amide I Contour in the IR Spectra of Globular Proteins: From Structure to Spectrum","volume":"127","author":"Brauner","year":"2005","journal-title":"J. Am. Chem. Soc."},{"key":"ref_43","unstructured":"Kurt Oldenburg (2023, August 29). LoadSpectra. Available online: https:\/\/www.mathworks.com\/matlabcentral\/fileexchange\/57904-loadspectra."},{"key":"ref_44","first-page":"091403","article-title":"Multimodal Label-Free Ex Vivo Imaging Using a Dual-Wavelength Microscope with Axial Chromatic Aberration Compensation","volume":"23","author":"Filippi","year":"2018","journal-title":"J. Biomed. Opt."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An Open-Source Platform for Biological-Image Analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Borile, G., Sandrin, D., Filippi, A., Anderson, K.I., and Romanato, F. (2021). Label-Free Multiphoton Microscopy: Much More Than Fancy Images. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22052657"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Zouhair, S., Dal Sasso, E., Tuladhar, S.R., Fidalgo, C., Vedovelli, L., Filippi, A., Borile, G., Bagno, A., Marchesan, M., and De Rossi, G. (2020). A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications. Biomolecules, 10.","DOI":"10.3390\/biom10030371"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Casarin, M., Fortunato, T.M., Imran, S., Todesco, M., Sandrin, D., Borile, G., Toniolo, I., Marchesan, M., Gerosa, G., and Bagno, A. (2022). Porcine Small Intestinal Submucosa (SIS) as a Suitable Scaffold for the Creation of a Tissue-Engineered Urinary Conduit: Decellularization, Biomechanical and Biocompatibility Characterization Using New Approaches. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23052826"},{"key":"ref_49","unstructured":"(2005). E.P. Commission Council of Europe 2.6.1. European Pharmacopeia 5.0, 2.6\u2014Biological Tests; 2.6.1 Sterility. Eur. Pharmacopoeia, 5.0, 145\u2013149."},{"key":"ref_50","unstructured":"(2009). Biological Evaluation of Medical Devices; Part 5, Tests for in Vitro Cytotoxicity. Standard No. ISO 10993-5:2009."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Huang, C.-C., Chen, Y.-J., and Liu, H.-W. (2021). Characterization of Composite Nano-Bioscaffolds Based on Collagen and Supercritical Fluids-Assisted Decellularized Fibrous Extracellular Matrix. Polymers, 13.","DOI":"10.3390\/polym13244326"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Huang, C.-C. (2021). Characteristics and Preparation of Designed Alginate-Based Composite Scaffold Membranes with Decellularized Fibrous Micro-Scaffold Structures from Porcine Skin. Polymers, 13.","DOI":"10.3390\/polym13203464"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1002\/bip.360271105","article-title":"Fourier Transform Ir Spectroscopy of Collagen and Gelatin Solutions: Deconvolution of the Amide I Band for Conformational Studies","volume":"27","author":"Payne","year":"1988","journal-title":"Biopolymers"},{"key":"ref_54","unstructured":"Twardowski, J., Anzenbacher, P., and Masson, M.R. (1994). Raman and IR Spectroscopy in Biology and Biochemistry, Polish Scientific Publishers. Ellis Horwood Series in Analytical Chemistry."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.avsg.2010.11.007","article-title":"Current Usage and Future Directions for the Bovine Pericardial Patch","volume":"25","author":"Li","year":"2011","journal-title":"Ann. Vasc. Surg."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3233","DOI":"10.1016\/j.biomaterials.2011.01.057","article-title":"An Overview of Tissue and Whole Organ Decellularization Processes","volume":"32","author":"Crapo","year":"2011","journal-title":"Biomaterials"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1038\/nrcardio.2013.77","article-title":"Small-Diameter Vascular Tissue Engineering","volume":"10","author":"Seifu","year":"2013","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Chrisikou, I., Orkoula, M., and Kontoyannis, C. (2020). FT-IR\/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam Formulation. Molecules, 25.","DOI":"10.3390\/molecules25246051"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Camas\u00e3o, D.B., and Mantovani, D. (2021). The Mechanical Characterization of Blood Vessels and Their Substitutes in the Continuous Quest for Physiological-Relevant Performances. A Critical Review. Mater. Today Bio, 10.","DOI":"10.1016\/j.mtbio.2021.100106"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.actbio.2013.08.022","article-title":"Electrospun Scaffolds for Tissue Engineering of Vascular Grafts","volume":"10","author":"Hasan","year":"2014","journal-title":"Acta Biomater."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/0021-9290(79)90039-3","article-title":"Mechanical Properties of Synthetic Arterial Grafts","volume":"12","author":"Hasegawa","year":"1979","journal-title":"J. Biomech."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1089\/ten.tea.2008.0183","article-title":"Dynamic Straining Combined with Fibrin Gel Cell Seeding Improves Strength of Tissue-Engineered Small-Diameter Vascular Grafts","volume":"15","author":"Stekelenburg","year":"2009","journal-title":"Tissue Eng. Part A"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1106\/NA5T-J57A-JTDD-FD04","article-title":"The Mechanical Behavior of Vascular Grafts: A Review","volume":"15","author":"Salacinski","year":"2001","journal-title":"J. Biomater. 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