{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T16:19:35Z","timestamp":1782749975451,"version":"3.54.5"},"reference-count":95,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T00:00:00Z","timestamp":1714348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005632","name":"The National Centre for Research and Development","doi-asserted-by":"publisher","award":["TECHMATSTRATEG-III\/0027\/2019"],"award-info":[{"award-number":["TECHMATSTRATEG-III\/0027\/2019"]}],"id":[{"id":"10.13039\/501100005632","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>There is a growing interest in the production of bioinks that on the one hand, are biocompatible and, on the other hand, have mechanical properties that allow for the production of stable constructs that can survive for a long time after transplantation. While the selection of the right material is crucial for bioprinting, there is another equally important issue that is currently being extensively researched\u2014the incorporation of the vascular system into the fabricated scaffolds. Therefore, in the following manuscript, we present the results of research on bioink with unique physico-chemical and biological properties. In this article, two methods of seeding cells were tested using bioink B and seeding after bioprinting the whole model. After 2, 5, 8, or 24 h of incubation, the flow medium was used in the tested systems. At the end of the experimental trial, for each time variant, the canals were stored in formaldehyde, and immunohistochemical staining was performed to examine the presence of cells on the canal walls and roof. Cells adhered to both ways of fiber arrangement; however, a parallel bioprint with the 5 h incubation and the intermediate plating of cells resulted in better adhesion efficiency. For this test variant, the percentage of cells that adhered was at least 20% higher than in the other analyzed variants. In addition, it was for this variant that the lowest percentage of viable cells was found that were washed out of the tested model. Importantly, hematoxylin and eosin staining showed that after 8 days of culture, the cells were evenly distributed throughout the canal roof. Our study clearly shows that neovascularization-promoting cells effectively adhere to ECM-based pancreatic bioink. Summarizing the presented results, it was demonstrated that the proposed bioink compositions can be used for bioprinting bionic organs with a vascular system formed by endothelial cells and fibroblasts.<\/jats:p>","DOI":"10.3390\/bioengineering11050439","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T09:50:07Z","timestamp":1714470607000},"page":"439","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Bioprinting of Perfusable, Biocompatible Vessel-like Channels with dECM-Based Bioinks and Living Cells"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3901-586X","authenticated-orcid":false,"given":"Marta","family":"Klak","sequence":"first","affiliation":[{"name":"Foundation of Research and Science Development, 01-242 Warsaw, Poland"},{"name":"Polbionica sp. z o.o., 01-242 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9701-8164","authenticated-orcid":false,"given":"Micha\u0142","family":"Rachalewski","sequence":"additional","affiliation":[{"name":"Foundation of Research and Science Development, 01-242 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Filip","sequence":"additional","affiliation":[{"name":"Foundation of Research and Science Development, 01-242 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Dobrza\u0144ski","sequence":"additional","affiliation":[{"name":"Polbionica sp. z o.o., 01-242 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Berman","sequence":"additional","affiliation":[{"name":"Polbionica sp. z o.o., 01-242 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Wszo\u0142a","sequence":"additional","affiliation":[{"name":"Foundation of Research and Science Development, 01-242 Warsaw, Poland"},{"name":"Polbionica sp. z o.o., 01-242 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10608","DOI":"10.1021\/acs.chemrev.9b00808","article-title":"Decellularized Extracellular Matrix-Based Bioinks for Engineering Tissue- and Organ-Specific Microenvironments","volume":"120","author":"Kim","year":"2020","journal-title":"Chem. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s13534-020-00171-8","article-title":"Freeform 3D Printing of Soft Matters: Recent Advances in Technology for Biomedical Engineering","volume":"10","author":"Chen","year":"2020","journal-title":"Biomed. Eng. Lett."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"549","DOI":"10.2217\/rme-2020-0091","article-title":"Vascularization Strategies for Tissue Engineering for Tracheal Reconstruction","volume":"16","author":"Sun","year":"2021","journal-title":"Regen. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.22203\/eCM.v014a01","article-title":"Engineering Thick Tissues\u2014The Vascularisation Problem","volume":"14","author":"Ko","year":"2007","journal-title":"Eur. Cells Mater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1038\/nbt0705-821","article-title":"Engineering Vascularized Tissue","volume":"23","author":"Jain","year":"2005","journal-title":"Nat. Biotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1002\/bit.27641","article-title":"Effect of Alginate Matrix Engineered to Mimic the Pancreatic Microenvironment on Encapsulated Islet Function","volume":"118","author":"Enck","year":"2021","journal-title":"Biotechnol. Bioeng."},{"key":"ref_7","first-page":"3675","article-title":"Decellularization of Tissues and Organs","volume":"27","author":"Gilbert","year":"2006","journal-title":"Biomaterials"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3233","DOI":"10.1016\/j.biomaterials.2011.01.057","article-title":"An Overview of Tissue and Whole Organ Decellularization Processes","volume":"32","author":"Crapo","year":"2011","journal-title":"Biomaterials"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e2005476","DOI":"10.1002\/adma.202005476","article-title":"Extracellular-Matrix-Reinforced Bioinks for 3D Bioprinting Human Tissue","volume":"33","author":"Alsafadi","year":"2021","journal-title":"Adv. Mater."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1097\/MOT.0000000000000691","article-title":"Extracellular Matrix-Based Hydrogels Obtained from Human Tissues: A Work Still in Progress","volume":"24","author":"Gazia","year":"2019","journal-title":"Curr. Opin. Organ Transplant."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1016\/j.biomaterials.2004.04.042","article-title":"Production and Characterization of ECM Powder: Implications for Tissue Engineering Applications","volume":"26","author":"Gilbert","year":"2005","journal-title":"Biomaterials"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.tibtech.2018.03.003","article-title":"Organ-Derived Decellularized Extracellular Matrix: A Game Changer for Bioink Manufacturing?","volume":"36","author":"Choudhury","year":"2018","journal-title":"Trends Biotechnol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.jacbts.2019.07.012","article-title":"First-in-Man Study of a Cardiac Extracellular Matrix Hydrogel in Early and Late Myocardial Infarction Patients","volume":"4","author":"Traverse","year":"2019","journal-title":"JACC Basic Transl. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.biomaterials.2016.10.026","article-title":"3D Printed Complex Tissue Construct Using Stem Cell-Laden Decellularized Extracellular Matrix Bioinks for Cardiac Repair","volume":"112","author":"Jang","year":"2017","journal-title":"Biomaterials"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1089\/ten.tea.2008.0587","article-title":"Maintenance of Human Hepatocyte Function in Vitro by Liver-Derived Extracellular Matrix Gels","volume":"16","author":"Sellaro","year":"2010","journal-title":"Tissue Eng.-Part A"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1126\/science.1189345","article-title":"Tissue-Engineered Lungs for in Vivo Implantation","volume":"329","author":"Petersen","year":"2010","journal-title":"Science"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4715","DOI":"10.1016\/j.biomaterials.2010.02.046","article-title":"The Use of Decellularized Adipose Tissue to Provide an Inductive Microenvironment for the Adipogenic Differentiation of Human Adipose-Derived Stem Cells","volume":"31","author":"Flynn","year":"2010","journal-title":"Biomaterials"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Bertassoni, L.E., Cardoso, J.C., Manoharan, V., Cristino, A.L., Bhise, N.S., Araujo, W.A., Zorlutuna, P., Vrana, N.E., Ghaemmaghami, A.M., and Dokmeci, M.R. (2014). Direct-Write Bioprinting of Cell-Laden Methacrylated Gelatin Hydrogels. Biofabrication, 6.","DOI":"10.1088\/1758-5082\/6\/2\/024105"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1021\/acsabm.0c00169","article-title":"3D Bioprinting of Methylcellulose\/Gelatin-Methacryloyl (MC\/GelMA) Bioink with High Shape Integrity","volume":"3","author":"Rastin","year":"2020","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1002\/jbm.a.36859","article-title":"3D-Engineered GelMA Conduit Filled with ECM Promotes Regeneration of Peripheral Nerve","volume":"108","author":"Gong","year":"2020","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.biomaterials.2015.08.045","article-title":"Synthesis, Properties, and Biomedical Applications of Gelatin Methacryloyl (GelMA) Hydrogels","volume":"73","author":"Yue","year":"2015","journal-title":"Biomaterials"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Adib, A.A., Sheikhi, A., Shahhosseini, M., Simeunovi\u0107, A., Wu, S., Castro, C.E., Zhao, R., Khademhosseini, A., and Hoelzle, D.J. (2020). Direct-Write 3D Printing and Characterization of a GelMA-Based Biomaterial for Intracorporeal Tissue Engineering. Biofabrication, 12.","DOI":"10.1088\/1758-5090\/ab97a1"},{"key":"ref_23","first-page":"181","article-title":"Stem Cells and Cell Therapies in Lung Biology and Diseases: Conference Report","volume":"12","author":"Fomby","year":"2010","journal-title":"Ann. Am. Thorac. Soc."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"353","DOI":"10.3390\/ma2020353","article-title":"Biodegradable Cellulose-Based Hydrogels: Design and Applications","volume":"2","author":"Sannino","year":"2009","journal-title":"Materials"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.1021\/nn507488s","article-title":"Bioactive Nanoengineered Hydrogels for Bone Tissue Engineering: A Growth-Factor-Free Approach","volume":"9","author":"Xavier","year":"2015","journal-title":"ACS Nano"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1002\/mabi.201200471","article-title":"Gelatin-Methacrylamide Hydrogels as Potential Biomaterials for Fabrication of Tissue-Engineered Cartilage Constructs","volume":"13","author":"Schuurman","year":"2013","journal-title":"Macromol. Biosci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1752","DOI":"10.1021\/acsbiomaterials.6b00149","article-title":"New Visible-Light Photoinitiating System for Improved Print Fidelity in Gelatin-Based Bioinks","volume":"2","author":"Lim","year":"2016","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2282","DOI":"10.1039\/c3tb21280g","article-title":"Development and Characterisation of a New Bioink for Additive Tissue Manufacturing","volume":"2","author":"Melchels","year":"2014","journal-title":"J. Mater. Chem. B"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1002\/term.2058","article-title":"Three-Dimensional Plotting of a Cell-Laden Alginate\/Methylcellulose Blend: Towards Biofabrication of Tissue Engineering Constructs with Clinically Relevant Dimensions","volume":"11","author":"Placht","year":"2017","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1021\/acsapm.8b00165","article-title":"Photopolymerization in 3D Printing","volume":"1","author":"Bagheri","year":"2019","journal-title":"ACS Appl. Polym. Mater."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Klak, M., Gom\u00f3\u0142ka, M., Dobrza\u0144ski, T., Tymicki, G., Cywoniuk, P., Kowalska, P., Kosowska, K., Bryniarski, T., Berman, A., and Dobrzy\u0144, A. (2020). Irradiation with 365 Nm and 405 Nm Wavelength Shows Differences in DNA Damage of Swine Pancreatic Islets. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0235052"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6702","DOI":"10.1016\/j.biomaterials.2009.08.055","article-title":"Photoinitiated Polymerization of PEG-Diacrylate with Lithium Phenyl-2,4,6-Trimethylbenzoylphosphinate: Polymerization Rate and Cytocompatibility","volume":"30","author":"Fairbanks","year":"2009","journal-title":"Biomaterials"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"110625","DOI":"10.1016\/j.msec.2020.110625","article-title":"Fabrication of Liver Microtissue with Liver Decellularized Extracellular Matrix (DECM) Bioink by Digital Light Processing (DLP) Bioprinting","volume":"109","author":"Mao","year":"2020","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2236","DOI":"10.1242\/jcs.163774","article-title":"Autocrine VEGF Maintains Endothelial Survival through Regulation of Metabolism and Autophagy","volume":"128","author":"Domigan","year":"2015","journal-title":"J. Cell Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1007\/s11033-020-06108-9","article-title":"Understanding Angiogenesis and the Role of Angiogenic Growth Factors in the Vascularisation of Engineered Tissues","volume":"48","author":"Omorphos","year":"2021","journal-title":"Mol. Biol. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Yang, G., Mahadik, B., Choi, J.Y., and Fisher, J.P. (2020). Vascularization in Tissue Engineering: Fundamentals and State-of-Art. Prog. Biomed. Eng., 2.","DOI":"10.1088\/2516-1091\/ab5637"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1089\/ten.2006.0225","article-title":"Stimulation of Peri-Implant Vascularization with Bone Marrow-Derived Progenitor Cells: Monitoring by In Vivo EPR Oximetry","volume":"13","author":"Butt","year":"2007","journal-title":"Tissue Eng."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.tibtech.2016.03.002","article-title":"Vascularization and Angiogenesis in Tissue Engineering: Beyond Creating Static Networks","volume":"34","author":"Rouwkema","year":"2016","journal-title":"Trends Biotechnol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1002\/dvdy.385","article-title":"Advances in Tissue Engineering of Vasculature through Three-Dimensional Bioprinting","volume":"250","author":"Zhu","year":"2021","journal-title":"Dev. Dyn."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Tomasina, C., Bodet, T., Mota, C., Moroni, L., and Camarero-espinosa, S. (2019). Despite the Great Advances That the Tissue Engineering Field Has. Materials, 12.","DOI":"10.3390\/ma12172701"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"6221","DOI":"10.1016\/j.biomaterials.2009.07.056","article-title":"Human Microvasculature Fabrication Using Thermal Inkjet Printing Technology","volume":"30","author":"Cui","year":"2009","journal-title":"Biomaterials"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1801102","DOI":"10.1002\/adhm.201801102","article-title":"Coaxial Cell Printing of Freestanding, Perfusable, and Functional In Vitro Vascular Models for Recapitulation of Native Vascular Endothelium Pathophysiology","volume":"7","author":"Gao","year":"2018","journal-title":"Adv. Healthc. Mater."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2026","DOI":"10.1161\/ATVBAHA.117.309196","article-title":"Differentiation and Application of Induced Pluripotent Stem Cell? Derived Vascular Smooth Muscle Cells","volume":"37","author":"Maguire","year":"2017","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2003777","DOI":"10.1002\/adfm.202003777","article-title":"Transient Support from Fibroblasts Is Sufficient to Drive Functional Vascularization in Engineered Tissues","volume":"30","author":"Song","year":"2020","journal-title":"Adv. Funct. Mater."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3791","DOI":"10.1091\/mbc.e11-05-0393","article-title":"The Requirement for Fibroblasts in Angiogenesis: Fibroblast-Derived Matrix Proteins Are Essential for Endothelial Cell Lumen Formation","volume":"22","author":"Newman","year":"2011","journal-title":"Mol. Biol. Cell"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.1089\/ten.tea.2012.0631","article-title":"Prevascularization of a Gas-Foaming Macroporous Calcium Phosphate Cement Scaffold via Coculture of Human Umbilical Vein Endothelial Cells and Osteoblasts","volume":"19","author":"Xu","year":"2013","journal-title":"Tissue Eng. Part A"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1021\/acs.iecr.5b03462","article-title":"Approaches to Fabricating Multiple-Layered Vascular Scaffolds Using Hybrid Electrospinning and Thermally Induced Phase Separation Methods","volume":"55","author":"Mi","year":"2016","journal-title":"Ind. Eng. Chem. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1163\/156856208X386237","article-title":"A Collagen\/Smooth Muscle Cell-Incorporated Elastic Scaffold for Tissue-Engineered Vascular Grafts","volume":"20","author":"Park","year":"2009","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1038\/nmat3357","article-title":"Rapid Casting of Patterned Vascular Networks for Perfusable Engineered Three-Dimensional Tissues","volume":"11","author":"Miller","year":"2012","journal-title":"Nat. Mater."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3124","DOI":"10.1002\/adma.201305506","article-title":"3D Bioprinting of Vascularized, Heterogeneous Cell-Laden Tissue Constructs","volume":"26","author":"Kolesky","year":"2014","journal-title":"Adv. Mater."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2202","DOI":"10.1039\/C4LC00030G","article-title":"Hydrogel Bioprinted Microchannel Networks for Vascularization of Tissue Engineering Constructs","volume":"14","author":"Bertassoni","year":"2014","journal-title":"Lab Chip"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1700015","DOI":"10.1002\/adhm.201700015","article-title":"Bioprinted Osteogenic and Vasculogenic Patterns for Engineering 3D Bone Tissue","volume":"6","author":"Byambaa","year":"2017","journal-title":"Adv. Healthc. Mater."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"020902","DOI":"10.1115\/1.4024398","article-title":"Direct Bioprinting of Vessel-like Tubular Microfluidic Channels","volume":"4","author":"Zhang","year":"2013","journal-title":"J. Nanotechnol. Eng. Med."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Yu, Y., Chen, H., and Ozbolat, I.T. (2013). Characterization of Printable Cellular Micro-Fluidic Channels for Tissue Engineering. Biofabrication, 5.","DOI":"10.1088\/1758-5082\/5\/2\/025004"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"629313","DOI":"10.3389\/fcvm.2021.629313","article-title":"Blood Flow Within Bioengineered 3D Printed Vascular Constructs Using the Porcine Model","volume":"8","author":"Paulsen","year":"2021","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"8092","DOI":"10.1016\/j.biomaterials.2014.05.083","article-title":"Creating Perfused Functional Vascular Channels Using 3D Bio-Printing Technology","volume":"35","author":"Lee","year":"2014","journal-title":"Biomaterials"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.jmst.2020.03.022","article-title":"Biomaterials Affect Cell-Cell Interactions in Vitro in Tissue Engineering","volume":"63","author":"He","year":"2021","journal-title":"J. Mater. Sci. Technol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2007199","DOI":"10.1002\/adfm.202007199","article-title":"Engineering New Microvascular Networks On-Chip: Ingredients, Assembly, and Best Practices","volume":"31","author":"Tronolone","year":"2021","journal-title":"Adv. Funct. Mater."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1089\/ten.tea.2014.0443","article-title":"Fibroblast-Endothelial Partners for Vascularization Strategies in Tissue Engineering","volume":"21","author":"Ramalho","year":"2015","journal-title":"Tissue Eng. Part A"},{"key":"ref_60","first-page":"366","article-title":"Critical Considerations for Regeneration of Vascularized Composite Tissues","volume":"27","author":"Buchen","year":"2020","journal-title":"Tissue Eng. Part B Rev."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Klak, M., \u0141ojszczyk, I., Berman, A., Tymicki, G., Adamiok-Ostrowska, A., Sierakowski, M., Olkowski, R., Szczepankiewicz, A.A., Kami\u0144ski, A., and Dobrzy\u0144, A. (2021). Impact of Porcine Pancreas Decellularization Conditions on the Quality of Obtained Decm. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22137005"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"34845","DOI":"10.1038\/srep34845","article-title":"Bioprinting of 3D Convoluted Renal Proximal Tubules on Perfusable Chips","volume":"6","author":"Homan","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"25096","DOI":"10.1021\/acsami.6b07725","article-title":"A Versatile Method for Fabricating Tissue Engineering Scaffolds with a Three-Dimensional Channel for Prevasculature Networks","volume":"8","author":"Li","year":"2016","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1002\/adhm.201500792","article-title":"Development of 3D Microvascular Networks Within Gelatin Hydrogels Using Thermoresponsive Sacrificial Microfibers","volume":"5","author":"Lee","year":"2016","journal-title":"Adv. Healthc. Mater."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.actbio.2019.02.038","article-title":"3D Bioprinting of Complex Channels within Cell-Laden Hydrogels","volume":"95","author":"Ji","year":"2019","journal-title":"Acta Biomater."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.devcel.2009.01.013","article-title":"Cellular and molecular mechanisms of vascular lumen formation","volume":"16","author":"Davis","year":"2009","journal-title":"Dev. Cell"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"6712","DOI":"10.1073\/pnas.1221526110","article-title":"Biomimetic model to reconstitute angiogenic sprouting morphogenesis in vitro","volume":"110","author":"Nguyen","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"4407","DOI":"10.1016\/j.biomaterials.2009.05.004","article-title":"Crosstalk between Osteoblasts and Endothelial Cells Co-Cultured on a Polycaprolactone-Starch Scaffold and the in Vitro Development of Vascularization","volume":"30","author":"Santos","year":"2009","journal-title":"Biomaterials"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1701521","DOI":"10.1002\/smll.201701521","article-title":"Development of Endothelial Cell Networks in 3D Tissues by Combination of Melt Electrospinning Writing with Cell-Accumulation Technology","volume":"14","author":"Bertlein","year":"2018","journal-title":"Small"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1536","DOI":"10.1039\/C9BM01337G","article-title":"Polymer Microcapsules and Microbeads as Cell Carriers for: In Vivo Biomedical Applications","volume":"8","year":"2020","journal-title":"Biomater. Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1002\/bit.27250","article-title":"ECM Concentration and Cell-Mediated Traction Forces Play a Role in Vascular Network Assembly in 3D Bioprinted Tissue","volume":"117","author":"Zhang","year":"2020","journal-title":"Biotechnol. Bioeng."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Wright, M.E., Yu, J.K., Jain, D., Maeda, A., Yeh, S.C.A., DaCosta, R.S., Lin, C.P., and Santerre, J.P. (2020). Engineering Functional Microvessels in Synthetic Polyurethane Random-Pore Scaffolds by Harnessing Perfusion Flow. Biomaterials, 256.","DOI":"10.1016\/j.biomaterials.2020.120183"},{"key":"ref_73","first-page":"e57826","article-title":"Bioprintable Alginate\/Gelatin Hydrogel 3D in Vitro Model Systems Induce Cell Spheroid Formation","volume":"2018","author":"Jiang","year":"2018","journal-title":"J. Vis. Exp."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"133","DOI":"10.3390\/microarrays4020133","article-title":"3D Cell Culture in Alginate Hydrogels","volume":"4","author":"Andersen","year":"2015","journal-title":"Microarrays"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s42242-018-0028-8","article-title":"Three-Dimensional Bioprinting of Gelatin Methacryloyl (GelMA)","volume":"1","author":"Ying","year":"2018","journal-title":"Bio-Design Manuf."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"6849","DOI":"10.1021\/acsami.7b16059","article-title":"3D Bioprinting of Low-Concentration Cell-Laden Gelatin Methacrylate (GelMA) Bioinks with a Two-Step Cross-Linking Strategy","volume":"10","author":"Yin","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.biomaterials.2013.09.078","article-title":"The 3D Printing of Gelatin Methacrylamide Cell-Laden Tissue-Engineered Constructs with High Cell Viability","volume":"35","author":"Billiet","year":"2014","journal-title":"Biomaterials"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Choi, Y.J., Park, H., Ha, D.H., Yun, H.S., Yi, H.G., and Lee, H. (2021). 3D Bioprinting of in Vitro Models Using Hydrogel-Based Bioinks. Polymers, 13.","DOI":"10.3390\/polym13030366"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"6832","DOI":"10.1039\/C5RA21527G","article-title":"Nano Hydroxyapatite Particles Promote Osteogenesis in a Three-Dimensional Bio-Printing Construct Consisting of Alginate\/Gelatin\/HASCs","volume":"6","author":"Wang","year":"2016","journal-title":"RSC Adv."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Kumar, A., I Matari, I.A., and Han, S.S. (2020). 3D Printable Carboxylated Cellulose Nanocrystal-Reinforced Hydrogel Inks for Tissue Engineering. Biofabrication, 12.","DOI":"10.1088\/1758-5090\/ab736e"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1089\/wound.2014.0561","article-title":"Extracellular Matrix and Dermal Fibroblast Function in the Healing Wound","volume":"5","author":"Tracy","year":"2016","journal-title":"Adv. Wound Care"},{"key":"ref_82","first-page":"1","article-title":"Fibroblasts: The Arbiters of Extracellular Matrix Remodeling","volume":"91\u201392","author":"Barker","year":"2020","journal-title":"Matrix Biol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.actbio.2020.09.033","article-title":"Vascular Bioprinting with Enzymatically Degradable Bioinks via Multi-Material Projection-Based Stereolithography","volume":"117","author":"Thomas","year":"2020","journal-title":"Acta Biomater."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"3150","DOI":"10.1002\/bit.27838","article-title":"3D Bioprinting and Microscale Organization of Vascularized Tissue Constructs Using Collagen-based Bioink","volume":"118","author":"Muthusamy","year":"2021","journal-title":"Biotechnol. Bioeng."},{"key":"ref_85","first-page":"1","article-title":"Bioprinting 3D Angiogenesis Models with Laser Induced Side Transfer","volume":"Volume 11647","author":"Hooker","year":"2021","journal-title":"Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX, Proceedings of the SPIE BiOS 2021, San Francisco, CA, USA, 6\u201311 March 2021"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Mastrullo, V., Cathery, W., Velliou, E., Madeddu, P., and Campagnolo, P. (2020). Angiogenesis in Tissue Engineering: As Nature Intended?. Front. Bioeng. Biotechnol., 8.","DOI":"10.3389\/fbioe.2020.00188"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"123","DOI":"10.3389\/fphar.2014.00123","article-title":"Fibroblasts in Fibrosis: Novel Roles and Mediators","volume":"5","author":"Kendall","year":"2014","journal-title":"Front. Pharmacol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/jre.12799","article-title":"Cellular Ageing of Oral Fibroblasts Differentially Modulates Extracellular Matrix Organization","volume":"56","author":"Atkuru","year":"2021","journal-title":"J. Periodontal Res."},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Guo, F., Carter, D.E., Mukhopadhyay, A., and Leask, A. (2011). Gingival Fibroblasts Display Reduced Adhesion and Spreading on Extracellular Matrix: A Possible Basis for Scarless Tissue Repair?. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0027097"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"6655","DOI":"10.1038\/s41598-021-85742-0","article-title":"Identification of Extracellular Matrix Proteins Secreted by Human Dermal Fibroblasts Cultured in 3D Electrospun Scaffolds","volume":"11","author":"Buzzi","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Xu, Y., Hu, Y., Liu, C., Yao, H., Liu, B., and Mi, S. (2018). A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting Technology. Materials, 11.","DOI":"10.3390\/ma11091581"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1161\/CIRCRESAHA.119.311148","article-title":"The Extracellular Matrix in Ischemic and Nonischemic Heart Failure","volume":"125","author":"Frangogiannis","year":"2019","journal-title":"Circ. Res."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.matbio.2020.03.007","article-title":"Computational Model Predicts Paracrine and Intracellular Drivers of Fibroblast Phenotype after Myocardial Infarction","volume":"91\u201392","author":"Zeigler","year":"2020","journal-title":"Matrix Biol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.matbio.2020.04.002","article-title":"Regulators of Cardiac Fibroblast Cell State","volume":"91\u201392","author":"Bretherton","year":"2020","journal-title":"Matrix Biol."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1126\/science.1176009","article-title":"The extracellular matrix: Not just pretty fibrils","volume":"326","author":"Hynesa","year":"2009","journal-title":"Science"}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/11\/5\/439\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:36:05Z","timestamp":1760106965000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/11\/5\/439"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,29]]},"references-count":95,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["bioengineering11050439"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering11050439","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,29]]}}}