{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T07:41:48Z","timestamp":1767339708677,"version":"build-2065373602"},"reference-count":84,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T00:00:00Z","timestamp":1723507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008871","name":"Oxford NIHR Biomedical Research Centre Theme and a Juvenile Diabetes Research Foundation (JDRF)","doi-asserted-by":"publisher","award":["31-2008-617","645991"],"award-info":[{"award-number":["31-2008-617","645991"]}],"id":[{"id":"10.13039\/100008871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Diabetes Research and Wellness Foundation (DRWF)","award":["31-2008-617","645991"],"award-info":[{"award-number":["31-2008-617","645991"]}]},{"name":"European Union\u2019s Horizon 2020","award":["31-2008-617","645991"],"award-info":[{"award-number":["31-2008-617","645991"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Throughout the isolation process, human islets are subjected to destruction of the islet basement membrane (BM) and reduced oxygen supply. Reconstruction of the BM represents an option to improve islet function and survival post-transplant and may particularly be relevant for islet encapsulation devices and scaffolds. In the present study, we assessed whether Perlecan, used alone or combined with the BM proteins (BMPs) Collagen-IV and Laminin-521, has the ability to protect isolated human islets from hypoxia-induced damage. Islets isolated from the pancreas of seven different organ donors were cultured for 4\u20135 days at 2% oxygen in plain CMRL (sham-treated controls) or in CMRL supplemented with BMPs used either alone or in combination. Postculture, islets were characterized regarding survival, in vitro function and production of chemokines and reactive oxygen species (ROS). Individually added BMPs significantly doubled islet survival and increased in vitro function. Combining BMPs did not provide a synergistic effect. Among the tested BMPs, Perlecan demonstrated the significantly strongest inhibitory effect on chemokine and ROS production when compared with sham-treatment (p &lt; 0.001). Perlecan may be useful to improve islet survival prior to and after transplantation. Its anti-inflammatory potency should be considered to optimise encapsulation and scaffolds to protect isolated human islets post-transplant.<\/jats:p>","DOI":"10.3390\/bioengineering11080828","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T03:46:36Z","timestamp":1723607196000},"page":"828","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Perlecan: An Islet Basement Membrane Protein with Protective Anti-Inflammatory Characteristics"],"prefix":"10.3390","volume":"11","author":[{"given":"Daniel","family":"Brandhorst","sequence":"first","affiliation":[{"name":"Islet Transplant Research Group, Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX3 9DU, UK"},{"name":"Oxford Consortium for Islet Transplantation, Oxford Centre for Diabetes, Endocrinology, and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford OX3 7LE, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heide","family":"Brandhorst","sequence":"additional","affiliation":[{"name":"Islet Transplant Research Group, Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX3 9DU, UK"},{"name":"Oxford Consortium for Islet Transplantation, Oxford Centre for Diabetes, Endocrinology, and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford OX3 7LE, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5117-4447","authenticated-orcid":false,"given":"Samuel","family":"Acreman","sequence":"additional","affiliation":[{"name":"Islet Transplant Research Group, Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX3 9DU, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3142-137X","authenticated-orcid":false,"given":"Paul R. V.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Islet Transplant Research Group, Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX3 9DU, UK"},{"name":"Oxford Consortium for Islet Transplantation, Oxford Centre for Diabetes, Endocrinology, and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford OX3 7LE, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/s41574-022-00790-4","article-title":"A spectacular year for islet and stem cell transplantation","volume":"19","author":"Shapiro","year":"2023","journal-title":"Nat. Rev. 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