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Modulating the sclera\u2019s properties by exogenous crosslinking offers a way to expand the tissue\u2019s range of properties for study of the possible influences on the eye\u2019s behavior and diseases such as glaucoma and myopia. The focus of this work was to evaluate the effects of genipin crosslinking targeting the porcine perilimbal sclera (PLS) since the stiffness of this tissue was previously found in a number of studies to influence the eye\u2019s intraocular pressure (IOP). The work includes experiments on tensile test specimens and whole globes. The specimen tests showed decreased strain-rate dependence and increased relaxation stress due to the cross-linker. Whole globe perfusion experiments demonstrated that eyes treated with genipin in the perilimbal region had increased IOPs compared to the control globes. Migration of the cross-linker from the target tissue to other tissues is a confounding factor in whole globe, biomechanical measurements, with crosslinking. A novel quantitative genipin assay of the trabecular meshwork (TM) was developed to exclude globes where the TM was inadvertently crosslinked. The perfusion study, therefore, suggests that elevated stiffness of the PLS can significantly increase IOP apart from effects of the TM in the porcine eye. These results demonstrate the importance of PLS biomechanics in aqueous outflow regulation and support additional investigations into the distal outflow pathways as a key source of outflow resistance.<\/jats:p>","DOI":"10.3390\/bioengineering11100996","type":"journal-article","created":{"date-parts":[[2024,10,2]],"date-time":"2024-10-02T11:29:24Z","timestamp":1727868564000},"page":"996","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of Genipin Crosslinking of Porcine Perilimbal Sclera on Mechanical Properties and Intraocular Pressure"],"prefix":"10.3390","volume":"11","author":[{"given":"John","family":"Riesterer","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering, University of Michigan-Dearborn, 4901 Evergreen Road, Dearborn, MI 48128, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexus","family":"Warchock","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, University of Michigan-Dearborn, 4901 Evergreen Road, Dearborn, MI 48128, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik","family":"Krawczyk","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, University of Michigan-Dearborn, 4901 Evergreen Road, Dearborn, MI 48128, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linyu","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4351-0321","authenticated-orcid":false,"given":"Wonsuk","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, University of Michigan-Dearborn, 4901 Evergreen Road, Dearborn, MI 48128, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sayoko E.","family":"Moroi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Havener Eye Institute, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA"},{"name":"Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan, Ann Abor, MI 48105, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Argento","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, University of Michigan-Dearborn, 4901 Evergreen Road, Dearborn, MI 48128, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/S0014-4835(03)00212-4","article-title":"Scleral structure, organisation and disease. 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