{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T22:40:05Z","timestamp":1783464005146,"version":"3.55.0"},"reference-count":47,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T00:00:00Z","timestamp":1734134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Knut and Alice Wallenberg foundation","award":["2017.0363"],"award-info":[{"award-number":["2017.0363"]}]},{"name":"Knut and Alice Wallenberg foundation","award":["2020-09"],"award-info":[{"award-number":["2020-09"]}]},{"name":"Knut and Alice Wallenberg foundation","award":["2024-03487"],"award-info":[{"award-number":["2024-03487"]}]},{"name":"Knut and Alice Wallenberg foundation","award":["ALFGBG-965535"],"award-info":[{"award-number":["ALFGBG-965535"]}]},{"name":"Jane and Dan Olsson Foundation for Science","award":["2017.0363"],"award-info":[{"award-number":["2017.0363"]}]},{"name":"Jane and Dan Olsson Foundation for Science","award":["2020-09"],"award-info":[{"award-number":["2020-09"]}]},{"name":"Jane and Dan Olsson Foundation for Science","award":["2024-03487"],"award-info":[{"award-number":["2024-03487"]}]},{"name":"Jane and Dan Olsson Foundation for Science","award":["ALFGBG-965535"],"award-info":[{"award-number":["ALFGBG-965535"]}]},{"name":"Swedish research council","award":["2017.0363"],"award-info":[{"award-number":["2017.0363"]}]},{"name":"Swedish research council","award":["2020-09"],"award-info":[{"award-number":["2020-09"]}]},{"name":"Swedish research council","award":["2024-03487"],"award-info":[{"award-number":["2024-03487"]}]},{"name":"Swedish research council","award":["ALFGBG-965535"],"award-info":[{"award-number":["ALFGBG-965535"]}]},{"name":"ALF-agreement","award":["2017.0363"],"award-info":[{"award-number":["2017.0363"]}]},{"name":"ALF-agreement","award":["2020-09"],"award-info":[{"award-number":["2020-09"]}]},{"name":"ALF-agreement","award":["2024-03487"],"award-info":[{"award-number":["2024-03487"]}]},{"name":"ALF-agreement","award":["ALFGBG-965535"],"award-info":[{"award-number":["ALFGBG-965535"]}]},{"name":"Hjalmar Svensson research foundation","award":["2017.0363"],"award-info":[{"award-number":["2017.0363"]}]},{"name":"Hjalmar Svensson research foundation","award":["2020-09"],"award-info":[{"award-number":["2020-09"]}]},{"name":"Hjalmar Svensson research foundation","award":["2024-03487"],"award-info":[{"award-number":["2024-03487"]}]},{"name":"Hjalmar Svensson research foundation","award":["ALFGBG-965535"],"award-info":[{"award-number":["ALFGBG-965535"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Transplantation of decellularized uterus tissue showed promise in supporting regeneration following uterine injury in animal models, suggesting an alternative to complete uterus transplantation for uterine factor infertility treatment. However, most animal studies utilized small grafts, limiting their clinical relevance. Hence, we used larger grafts (20 \u00d7 10 mm), equivalent to nearly one uterine horn in rats, to better evaluate the bioengineering challenges associated with structural support, revascularization, and tissue regeneration. We analyzed histopathology, employed immunohistochemistry, and investigated gene expression discrepancies in growth-related proteins over four months post-transplantation in acellular grafts and those recellularized (RC) with bone marrow-derived mesenchymal stem cells (bmMSCs). RC grafts exhibited less inflammation and faster epithelialization and migration of endogenous cells into the graft compared with acellular grafts. Despite the lack of a significant difference in the density of CD31 positive blood vessels between groups, the RC group demonstrated a better organized myometrial layer and an overall faster regenerative progress. Elevated gene expression for Vegf, Cd44, and Itgb1 correlated with the enhanced tissue regeneration in this group. Elevated Tgfb expression was noted in both groups, potentially contributing to the rapid revascularization. Our findings suggest that large uterine injuries can be regenerated using decellularized tissue, with bmMSCs enhancing the endogenous repair mechanisms.<\/jats:p>","DOI":"10.3390\/bioengineering11121268","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T04:14:52Z","timestamp":1734322492000},"page":"1268","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Uterine Repair Mechanisms Are Potentiated by Mesenchymal Stem Cells and Decellularized Tissue Grafts Through Elevated Vegf, Cd44, and Itgb1 Gene Expression"],"prefix":"10.3390","volume":"11","author":[{"given":"Sara","family":"Bandstein","sequence":"first","affiliation":[{"name":"Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"},{"name":"Department of Obstetrics and Gynecology, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6556-9108","authenticated-orcid":false,"given":"Lucia","family":"De Miguel-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"},{"name":"Department of Obstetrics and Gynecology, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edina","family":"Sehic","sequence":"additional","affiliation":[{"name":"Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"},{"name":"Department of Obstetrics and Gynecology, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emy","family":"Thor\u00e9n","sequence":"additional","affiliation":[{"name":"Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"},{"name":"Department of Obstetrics and Gynecology, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8114-0296","authenticated-orcid":false,"given":"Sara","family":"L\u00f3pez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, IVI Foundation, Instituto de Investigaci\u00f3n Sanitaria La Fe (IIS La Fe), Avenida Fernando Abril Martorell, 106, 46026 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7018-4971","authenticated-orcid":false,"given":"Irene","family":"Cervell\u00f3","sequence":"additional","affiliation":[{"name":"IVIRMA Global Research Alliance, IVI Foundation, Instituto de Investigaci\u00f3n Sanitaria La Fe (IIS La Fe), Avenida Fernando Abril Martorell, 106, 46026 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randa","family":"Akouri","sequence":"additional","affiliation":[{"name":"Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"},{"name":"Department of Obstetrics and Gynecology, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3783-5207","authenticated-orcid":false,"given":"Mihai","family":"Oltean","sequence":"additional","affiliation":[{"name":"Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"},{"name":"Department of Surgery, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-413 45 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6081-9101","authenticated-orcid":false,"given":"Mats","family":"Br\u00e4nnstr\u00f6m","sequence":"additional","affiliation":[{"name":"Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"},{"name":"Department of Obstetrics and Gynecology, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"},{"name":"Stockholm IVF-EUGIN, Hammarby all\u00e9 93, SE-120 63 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3323-5618","authenticated-orcid":false,"given":"Mats","family":"Hellstr\u00f6m","sequence":"additional","affiliation":[{"name":"Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"},{"name":"Department of Obstetrics and Gynecology, Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, SE-405 30 Gothenburg, Sweden"},{"name":"Health Innovation Labs by Sahlgrenska Science Park, Medicinaregatan 9C, SE-413 90 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1038\/s41536-021-00136-0","article-title":"Towards a bioengineered uterus: Bioactive sheep uterus scaffolds are effectively recellularized by enzymatic preconditioning","volume":"6","author":"Padma","year":"2021","journal-title":"NPJ Regen. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1007\/s10439-016-1779-z","article-title":"Biomaterial Scaffolds for Reproductive Tissue Engineering","volume":"45","author":"Peng","year":"2017","journal-title":"Ann. Biomed. Eng."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1093\/humrep\/deae148","article-title":"The importance of the \u2018uterine factor\u2019 in recurrent pregnancy loss: A retrospective cohort study on women screened through 3D transvaginal ultrasound","volume":"39","author":"Busnelli","year":"2024","journal-title":"Hum. Reprod."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.fertnstert.2016.03.048","article-title":"Bioengineered uterine tissue supports pregnancy in a rat model","volume":"106","author":"Bandstein","year":"2016","journal-title":"Fertil. Steril."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8791","DOI":"10.1016\/j.biomaterials.2014.06.052","article-title":"Partial regeneration and reconstruction of the rat uterus through recellularization of a decellularized uterine matrix","volume":"35","author":"Miyazaki","year":"2014","journal-title":"Biomaterials"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Santoso, E.G., Yoshida, K., Hirota, Y., Aizawa, M., Yoshino, O., Kishida, A., Osuga, Y., Saito, S., Ushida, T., and Furukawa, K.S. (2014). Application of detergents or high hydrostatic pressure as decellularization processes in uterine tissues and their subsequent effects on in vivo uterine regeneration in murine models. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0103201"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5034","DOI":"10.1016\/j.actbio.2014.08.018","article-title":"Towards the development of a bioengineered uterus: Comparison of different protocols for rat uterus decellularization","volume":"10","author":"Sihlbom","year":"2014","journal-title":"Acta Biomater."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e87591","DOI":"10.1172\/jci.insight.87591","article-title":"STAT3 accelerates uterine epithelial regeneration in a mouse model of decellularized uterine matrix transplantation","volume":"1","author":"Hiraoka","year":"2016","journal-title":"JCI Insight"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1080\/21691401.2019.1699828","article-title":"Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction","volume":"48","author":"Yao","year":"2020","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.actbio.2019.03.004","article-title":"Tissue-specific decellularized endometrial substratum mimicking different physiological conditions influences in vitro embryo development in a rabbit model","volume":"89","author":"Campo","year":"2019","journal-title":"Acta Biomater."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"105257","DOI":"10.1016\/j.rvsc.2024.105257","article-title":"Development of decellularization protocols for female cat reproductive organs","volume":"173","author":"Sanguansook","year":"2024","journal-title":"Res. Vet. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1095\/biolre\/bio143396","article-title":"De- and recellularization of the pig uterus: A bioengineering pilot study","volume":"96","author":"Campo","year":"2017","journal-title":"Biol. Reprod."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1007\/s10815-019-01463-4","article-title":"Development of an efficient perfusion-based protocol for whole-organ decellularization of the ovine uterus as a human-sized model and in vivo application of the bioscaffolds","volume":"36","author":"Daryabari","year":"2019","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1071\/RDv31n1Ab52","article-title":"Decellularization of goat uterus as a promising 3-dimensional homing matrix of biological scaffold: A pilot study","volume":"31","author":"Ghiringhelli","year":"2019","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s41231-024-00175-x","article-title":"Decellularization and enzymatic preconditioning of bovine uterus for improved recellularization","volume":"9","author":"Sehic","year":"2024","journal-title":"Transl. Med. Commun."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"De Miguel-G\u00f3mez, L., Sehic, E., Thor\u00e9n, E., Ahlstr\u00f6m, J., Rabe, H., Oltean, M., Br\u00e4nnstr\u00f6m, M., and Hellstr\u00f6m, M. (2024). Toward human uterus tissue engineering: Uterine decellularization in a non-human primate species. Acta Obstet. Gynecol. Scand.","DOI":"10.1111\/aogs.15030"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2112","DOI":"10.1089\/ten.tea.2012.0549","article-title":"Allo- and xeno-reassembly of human and rat myometrium from cells and scaffolds","volume":"19","author":"Young","year":"2013","journal-title":"Tissue Eng. Part. A"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1007\/s10815-022-02492-2","article-title":"Whole-organ decellularization of the human uterus and in vivo application of the bio-scaffolds in animal models","volume":"39","author":"Daryabari","year":"2022","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"20417314221118858","DOI":"10.1177\/20417314221118858","article-title":"Mesenchymal stem cells establish a pro-regenerative immune milieu after decellularized rat uterus tissue transplantation","volume":"13","author":"Sehic","year":"2022","journal-title":"J. Tissue Eng."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1002\/term.3217","article-title":"Decellularization protocol-dependent damage-associated molecular patterns in rat uterus scaffolds differentially affect the immune response after transplantation","volume":"15","author":"Padma","year":"2021","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2136","DOI":"10.1039\/D3BM01912H","article-title":"Transplantation of a bioengineered tissue patch promotes uterine repair in the sheep","volume":"12","author":"Sehic","year":"2024","journal-title":"Biomater. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1177\/0300985813485099","article-title":"Principles for valid histopathologic scoring in research","volume":"50","author":"Olivier","year":"2013","journal-title":"Vet. Pathol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Bankhead, P., Loughrey, M.B., Fern\u00e1ndez, J.A., Dombrowski, Y., McArt, D.G., Dunne, P.D., McQuaid, S., Gray, R.T., Murray, L.J., and Coleman, H.G. (2017). QuPath: Open source software for digital pathology image analysis. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-17204-5"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1373\/clinchem.2013.206375","article-title":"The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments","volume":"59","author":"Huggett","year":"2013","journal-title":"Clin. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4888","DOI":"10.1016\/j.biomaterials.2014.02.046","article-title":"Transplantation of bone marrow mesenchymal stem cells on collagen scaffolds for the functional regeneration of injured rat uterus","volume":"35","author":"Ding","year":"2014","journal-title":"Biomaterials"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1007\/s43032-021-00503-8","article-title":"Bioengineering of the Uterus","volume":"28","author":"Yoshimasa","year":"2021","journal-title":"Reprod. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"105657","DOI":"10.1016\/j.isci.2022.105657","article-title":"Biomaterializing the advances in uterine tissue engineering","volume":"25","author":"Wei","year":"2022","journal-title":"iScience"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1186\/s13287-019-1332-8","article-title":"Exosomes derived from mesenchymal stem cells reverse EMT via TGF-\u03b21\/Smad pathway and promote repair of damaged endometrium","volume":"10","author":"Yao","year":"2019","journal-title":"Stem Cell Res. Ther."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Jin, X., Dai, Y., Xin, L., Ye, Z., Chen, J., He, Q., Chen, X., Xu, X., Song, G., and Yu, X. (2023). ADSC-derived exosomes-coupled decellularized matrix for endometrial regeneration and fertility restoration. Mater. Today Bio, 23.","DOI":"10.1016\/j.mtbio.2023.100857"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.reprotox.2016.11.004","article-title":"The chick embryo chorioallantoic membrane (CAM) assay","volume":"70","author":"Ribatti","year":"2017","journal-title":"Reprod. Toxicol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Trigo, C.M., Rodrigues, J.S., Cam\u00f5es, S.P., Sol\u00e1, S., and Miranda, J.P. (2024). Mesenchymal stem cell secretome for regenerative medicine: Where do we stand?. J. Adv. Res., in press.","DOI":"10.1016\/j.jare.2024.05.004"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"13125","DOI":"10.1096\/fj.202000380RR","article-title":"The role of CD44 in pathological angiogenesis","volume":"34","author":"Chen","year":"2020","journal-title":"FASEB J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41392-022-01259-6","article-title":"Targeting integrin pathways: Mechanisms and advances in therapy","volume":"8","author":"Pang","year":"2023","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.1002\/jcsm.12767","article-title":"TMEM182 interacts with integrin beta 1 and regulates myoblast differentiation and muscle regeneration","volume":"12","author":"Luo","year":"2021","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1038\/cr.2008.326","article-title":"TGF-\u03b2 signaling in vascular biology and dysfunction","volume":"19","author":"Goumans","year":"2009","journal-title":"Cell Res."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Tie, Y., Tang, F., Peng, D., Zhang, Y., and Shi, H. (2022). TGF-beta signal transduction: Biology, function and therapy for diseases. Mol. Biomed., 3.","DOI":"10.1186\/s43556-022-00109-9"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1007\/s12265-024-10545-3","article-title":"HnRNPA1 Prevents Endothelial-to-mesenchymal Transition-induced VSMC Activation and Neointimal Hyperplasia in Vein Grafts","volume":"17","author":"Liu","year":"2024","journal-title":"J. Cardiovasc. Transl. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.jss.2012.11.009","article-title":"Creation of a female rabbit model for intrauterine adhesions using mechanical and infectious injury","volume":"183","author":"Liu","year":"2013","journal-title":"J. Surg. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/j.gendis.2022.08.009","article-title":"Endometrial stem\/progenitor cells: Properties, origins, and functions","volume":"10","author":"Hong","year":"2023","journal-title":"Genes Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1111\/wrr.12729","article-title":"Leukemia inhibitory factor promotes the regeneration of rat uterine horns with full-thickness injury","volume":"27","author":"Xue","year":"2019","journal-title":"Wound Repair Regen."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Gargett, C.E., and Hapangama, D. (2022). Endometrial Stem\/Progenitor Cells: Prospects and Challenges. J. Pers. Med., 12.","DOI":"10.3390\/jpm12091466"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1644","DOI":"10.1038\/s41591-020-1040-z","article-title":"Single-cell transcriptomic atlas of the human endometrium during the menstrual cycle","volume":"26","author":"Wang","year":"2020","journal-title":"Nat. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1186\/s13287-021-02354-1","article-title":"Single-cell RNA sequencing of cultured human endometrial CD140b(+)CD146(+) perivascular cells highlights the importance of in vivo microenvironment","volume":"12","author":"Cao","year":"2021","journal-title":"Stem Cell Res. Ther."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Boro\u0144, D., Zmarz\u0142y, N., Wierzbik-Stro\u0144ska, M., Rosi\u0144czuk, J., Mieszcza\u0144ski, P., and Grabarek, B.O. (2022). Recent Multiomics Approaches in Endometrial Cancer. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23031237"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"eadi6488","DOI":"10.1126\/sciadv.adi6488","article-title":"Promotion of uterine reconstruction by a tissue-engineered uterus with biomimetic structure and extracellular matrix microenvironment","volume":"9","author":"Zhao","year":"2023","journal-title":"Sci. Adv."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Juul, N., Amoushahi, M., Willacy, O., Ji, M., Villa, C., Ajalloueian, F., Chamorro, C., and Fossum, M. (2024). Autologous micrografting improves regeneration of tissue-engineered urinary conduits in vivo. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-72876-0"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e25275","DOI":"10.1016\/j.heliyon.2024.e25275","article-title":"A perioperative layered autologous tissue expansion graft for hollow organ repair","volume":"10","author":"Willacy","year":"2024","journal-title":"Heliyon"}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/11\/12\/1268\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:55:10Z","timestamp":1760115310000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/11\/12\/1268"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,14]]},"references-count":47,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["bioengineering11121268"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering11121268","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,14]]}}}