{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T04:54:29Z","timestamp":1772859269268,"version":"3.50.1"},"reference-count":56,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T00:00:00Z","timestamp":1741910400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University College Alberta Gim\u00e9nez (CESAG)","award":["PRO2023-002"],"award-info":[{"award-number":["PRO2023-002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Running-induced fatigue affects several biomechanical parameters, and yet few studies are focused on the acceleration spikes\u2019 asymmetries. This study aimed to evaluate the effects of a 30 min run on lower limbs spikes\u2019 asymmetries. Eighteen recreational runners (35.6 \u00b1 7.5 years; seven women) performed a treadmill running protocol at a moderate speed and acceleration spikes\u2019 asymmetries and kinematic (temporal) parameters were measured via accelerometers\u2014on the tibias and sacrum\u2014and photogrammetry. Acceleration spikes\u2019 parameters were continuously measured and averaged per minute to assess the relationship between fatigue and acceleration spike asymmetries via a linear regression model. Right tibial acceleration spikes increased over time (r = 0.9; p &lt; 0.001) and left tibia spikes decreased (r = 0.78; p &lt; 0.001), with a rise in tibial load asymmetry from 9% to 25% at the end (r = 0.98; p &lt; 0.001). This study suggest that fatigue affects the acceleration spikes of the two legs differently, with increasingly greater acceleration spikes in the right (dominant) leg. These findings should be considered, as greater asymmetries are related to overuse injuries and lower efficiency. Also, in studies focusing on running mechanics with fatigue, it is recommended that researchers collect data from both limbs, and not only from the right (dominant) leg.<\/jats:p>","DOI":"10.3390\/bioengineering12030294","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T07:02:16Z","timestamp":1741935736000},"page":"294","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Does the Fatigue Induced by a 30-Minute Run Affect the Lower Limb Acceleration Spikes\u2019 Asymmetries?"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3429-9755","authenticated-orcid":false,"given":"Gabriel","family":"Delgado-Garc\u00eda","sequence":"first","affiliation":[{"name":"SER Research Group, Physical Activity and Sport Sciences Department, CESAG, Comillas Pontifical University, 07013 Palma de Mallorca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2971-0642","authenticated-orcid":false,"given":"Isabel M.","family":"Mart\u00edn-L\u00f3pez","sequence":"additional","affiliation":[{"name":"SER Research Group, Physical Activity and Sport Sciences Department, CESAG, Comillas Pontifical University, 07013 Palma de Mallorca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8593-4037","authenticated-orcid":false,"given":"Fulgencio","family":"Soto-M\u00e9ndez","sequence":"additional","affiliation":[{"name":"SER Research Group, Physical Activity and Sport Sciences Department, CESAG, Comillas Pontifical University, 07013 Palma de Mallorca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0843-1097","authenticated-orcid":false,"given":"Arturo","family":"Qu\u00edlez-Maim\u00f3n","sequence":"additional","affiliation":[{"name":"SER Research Group, Physical Activity and Sport Sciences Department, CESAG, Comillas Pontifical University, 07013 Palma de Mallorca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8382-0609","authenticated-orcid":false,"given":"Salvador","family":"Boned-G\u00f3mez","sequence":"additional","affiliation":[{"name":"SER Research Group, Physical Activity and Sport Sciences Department, CESAG, Comillas Pontifical University, 07013 Palma de Mallorca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Krishan, K., Kanchan, T., and DiMaggio, J.A. (2010). A Study of Limb Asymmetry and Its Effect on Estimation of Stature in Forensic Case Work. Forensic Sci. Int., 200.","DOI":"10.1016\/j.forsciint.2010.04.015"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/S0966-6362(00)00070-9","article-title":"Symmetry and Limb Dominance in Able-Bodied Gait: A Review","volume":"12","author":"Sadeghi","year":"2000","journal-title":"Gait Posture"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1016\/j.gaitpost.2013.12.007","article-title":"The Effects of Limb Dominance and Fatigue on Running Biomechanics","volume":"39","author":"Brown","year":"2014","journal-title":"Gait Posture"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2792","DOI":"10.1016\/j.jbiomech.2005.10.003","article-title":"Kinetic Asymmetry in Female Runners with and without Retrospective Tibial Stress Fractures","volume":"39","author":"Zifchock","year":"2006","journal-title":"J. Biomech."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1016\/j.humov.2008.03.007","article-title":"Side-to-Side Differences in Overuse Running Injury Susceptibility: A Retrospective Study","volume":"27","author":"Zifchock","year":"2008","journal-title":"Hum. Mov. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2147","DOI":"10.1007\/s00421-018-3939-3","article-title":"Step Time Asymmetry Increases Metabolic Energy Expenditure during Running","volume":"118","author":"Beck","year":"2018","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.gaitpost.2019.12.018","article-title":"Does Fatigue Alter Step Characteristics and Stiffness during Running?","volume":"76","year":"2020","journal-title":"Gait Posture"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1007\/s00421-007-0456-1","article-title":"Preferred and Optimal Stride Frequency, Stiffness and Economy: Changes with Fatigue during a 1-h High-Intensity Run","volume":"100","author":"Hunter","year":"2007","journal-title":"Eur. J. Appl. Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0167-9457(00)00013-0","article-title":"Effect of Fatigue on Leg Kinematics and Impact Acceleration in Long Distance Running","volume":"19","author":"Mizrahi","year":"2000","journal-title":"Hum. Mov. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"M\u00f6hler, F., Fadillioglu, C., and Stein, T. (2021). Fatigue-Related Changes in Spatiotemporal Parameters, Joint Kinematics and Leg Stiffness in Expert Runners During a Middle-Distance Run. Front. Sports Act. Living, 3.","DOI":"10.3389\/fspor.2021.634258"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"M\u00f6hler, F., Stetter, B., M\u00fcller, H., and Stein, T. (2021). Stride-to-Stride Variability of the Center of Mass in Male Trained Runners After an Exhaustive Run: A Three Dimensional Movement Variability Analysis with a Subject-Specific Anthropometric Model. Front. Sports Act. Living, 3.","DOI":"10.3389\/fspor.2021.665500"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1519\/JSC.0000000000001253","article-title":"The Effect of Exertion and Sex on Vertical Ground Reaction Force Variables and Landing Mechanics","volume":"30","author":"Bell","year":"2016","journal-title":"J. Strength Cond. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.gaitpost.2016.11.005","article-title":"Asymmetry between Lower Limbs during Rested and Fatigued State Running Gait in Healthy Individuals","volume":"51","author":"Radzak","year":"2017","journal-title":"Gait Posture"},{"key":"ref_14","first-page":"29","article-title":"Vertical Ground Reaction Force Asymmetry in Prolonged Running","volume":"28","author":"Ryu","year":"2018","journal-title":"Korean J. Appl. Biomech."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Gao, Z., Fekete, G., Baker, J.S., Liang, M., Xuan, R., and Gu, Y. (2022). Effects of Running Fatigue on Lower Extremity Symmetry among Amateur Runners: From a Biomechanical Perspective. Front. Physiol., 13.","DOI":"10.3389\/fphys.2022.899818"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Mtibaa, K., Zarrouk, N., Ryu, J.H., Racinais, S., and Girard, O. (2023). Mechanical Asymmetries Remain Low-to-Moderate during 30 Min of Self-Paced Treadmill Running. Front. Physiol., 14.","DOI":"10.3389\/fphys.2023.1289172"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"310","DOI":"10.3390\/biomechanics3030027","article-title":"Fatigue Effects on Peak Plantar Pressure and Bilateral Symmetry during Gait at Various Speeds","volume":"3","author":"Buxton","year":"2023","journal-title":"Biomechanics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.humov.2017.02.008","article-title":"Lower Limb Mechanical Asymmetry during Repeated Treadmill Sprints","volume":"52","author":"Girard","year":"2017","journal-title":"Hum. Mov. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1080\/14763141.2020.1767188","article-title":"Asymmetries during Repeated Treadmill Sprints in Elite Female Rugby Sevens Players","volume":"22","author":"Girard","year":"2023","journal-title":"Sports Biomech."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1080\/14763141.2021.1900356","article-title":"Gait Asymmetries during Perceptually-Regulated Interval Running in Hypoxia and Normoxia","volume":"23","author":"Girard","year":"2024","journal-title":"Sports Biomech."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Girard, O., Millet, G.P., and Micallef, J.-P. (2024). Constant Low-to-Moderate Mechanical Asymmetries during 800-m Track Running. Front. Sports Act. Living, 6.","DOI":"10.3389\/fspor.2024.1278454"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.jbiomech.2018.08.008","article-title":"Gait Variability and Symmetry Remain Consistent during High-Intensity 10,000 m Treadmill Running","volume":"79","author":"Hanley","year":"2018","journal-title":"J. Biomech."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1080\/02640414.2020.1809176","article-title":"Running after Cycling Induces Inter-Limb Differences in Muscle Activation but Not in Kinetics or Kinematics","volume":"39","author":"Jacques","year":"2021","journal-title":"J. Sports Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Gao, Z., Mei, Q., Fekete, G., Baker, J.S., and Gu, Y. (2020). The Effect of Prolonged Running on the Symmetry of Biomechanical Variables of the Lower Limb Joints. Symmetry, 12.","DOI":"10.3390\/sym12050720"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1080\/02640414.2023.2268374","article-title":"Fatigue Does Not Increase Limb Asymmetry or Induce Proximal Joint Power Shift during Sprinting in Habitual, Multi-Speed Runners","volume":"41","author":"Vial","year":"2023","journal-title":"J. Sports Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1123\/jab.16.3.276","article-title":"Mechanical Modeling of Tibial Axial Accelerations Following Impulsive Heel Impact","volume":"16","author":"Andrews","year":"2000","journal-title":"J. Appl. Biomech."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3074","DOI":"10.1080\/14763141.2022.2086169","article-title":"Relative and Absolute Reliability of Shank and Sacral Running Impact Accelerations over a Short- and Long-Term Time Frame","volume":"23","author":"Burke","year":"2024","journal-title":"Sports Biomech."},{"key":"ref_28","first-page":"392","article-title":"Acceleration Spikes and Attenuation Response in the Trunk in Amateur Tennis Players during Real Game Actions","volume":"238","author":"Vanrenterghem","year":"2023","journal-title":"Proc. Inst. Mech. Eng. Part P J. Sports Eng. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1080\/14763141.2014.909527","article-title":"Effects of Treadmill Running and Fatigue on Impact Acceleration in Distance Running","volume":"13","year":"2014","journal-title":"Sports Biomech."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1080\/0264041031000140383","article-title":"Characteristics of Shock Attenuation during Fatigued Running","volume":"21","author":"Mercer","year":"2003","journal-title":"J. Sports Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1123\/jab.14.3.300","article-title":"Shock Transmission and Fatigue in Human Running","volume":"14","author":"Verbitsky","year":"1998","journal-title":"J. Appl. Biomech."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S0268-0033(98)00030-8","article-title":"Dynamic Loading on the Human Musculoskeletal System\u2014Effect of Fatigue","volume":"13","author":"Voloshin","year":"1998","journal-title":"Clin. Biomech."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.ergon.2009.02.003","article-title":"Assessment of Ventilatory Threshold Using Near-Infrared Spectroscopy on the Gastrocnemius Muscle during Treadmill Running","volume":"40","author":"Karatzanos","year":"2010","journal-title":"Int. J. Ind. Ergon."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1109\/TITB.2012.2201950","article-title":"Monitoring Kinematic Changes with Fatigue in Running Using Body-Worn Sensors","volume":"16","author":"Strohrmann","year":"2012","journal-title":"IEEE Trans. Inform. Technol. Biomed."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Encarnaci\u00f3n-Mart\u00ednez, A., P\u00e9rez-Soriano, P., Sanchis-Sanchis, R., Berenguer-Vidal, R., and Garc\u00eda-Gallart, A. (2021). Modification of Angular Kinematics and Spatiotemporal Parameters during Running after Central and Peripheral Fatigue. Appl. Sci., 11.","DOI":"10.3390\/app11146610"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/S0268-0033(03)00029-9","article-title":"Nonlinear Dynamics Indicates Aging Affects Variability during Gait","volume":"18","author":"Buzzi","year":"2003","journal-title":"Clin. Biomech."},{"key":"ref_37","first-page":"138","article-title":"Changes in Distance Running Kinematics with Fatigue","volume":"7","author":"Williams","year":"1991","journal-title":"J. Appl. Biomech."},{"key":"ref_38","unstructured":"Evans, J.D. (1996). Straightforward Statistics for the Behavioral Sciences, Brooks\/Cole Pub. Co."},{"key":"ref_39","unstructured":"Miller, D. (2020). Measurement by the Physical Educator: Why and How, McGrawHill. [7th ed.]."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Cohen, J., Cohen, P., West, S.G., and Aiken, L.S. (2013). Applied Multiple Regression\/Correlation Analysis for the Behavioral Sciences, Routledge.","DOI":"10.4324\/9780203774441"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1016\/j.jbiomech.2013.06.011","article-title":"Changes in Spring-Mass Behavior and Muscle Activity during an Exhaustive Run at V\u02d9O2max","volume":"46","author":"Rabita","year":"2013","journal-title":"J. Biomech."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2507","DOI":"10.1249\/MSS.0000000000001707","article-title":"Positive Work Contribution Shifts from Distal to Proximal Joints during a Prolonged Run","volume":"50","author":"Sanno","year":"2018","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"11","DOI":"10.4085\/1062-6050-46.1.11","article-title":"Muscle Coactivation Before and After the Impact Phase of Running Following Isokinetic Fatigue","volume":"46","author":"Kellis","year":"2011","journal-title":"J. Athl. Train."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1097\/00005768-200206000-00015","article-title":"Impacts and Kinematic Adjustments during an Exhaustive Run","volume":"34","author":"Derrick","year":"2002","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Chalitsios, C., Nikodelis, T., Mavrommatis, G., and Kollias, I. (2024). Subject-Specific Sensitivity of Several Biomechanical Features to Fatigue during an Exhaustive Treadmill Run. Sci. Rep., 14.","DOI":"10.1038\/s41598-024-51296-0"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1016\/S0021-9290(00)00064-6","article-title":"Stretch-Shortening Cycle: A Powerful Model to Study Normal and Fatigued Muscle","volume":"33","author":"Komi","year":"2000","journal-title":"J. Biomech."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.ptsp.2010.10.004","article-title":"Differences in Muscle Strength in Dominant and Non-Dominant Leg in Females Aged 20\u201339 Years\u2014A Population-Based Study","volume":"12","author":"Lanshammar","year":"2011","journal-title":"Phys. Ther. Sport"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Xiang, L., Gao, Z., Wang, A., Shim, V., Fekete, G., Gu, Y., and Fernandez, J. (2024). Rethinking Running Biomechanics: A Critical Review of Ground Reaction Forces, Tibial Bone Loading, and the Role of Wearable Sensors. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1377383"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Zandbergen, M.A., Ter Wengel, X.J., Van Middelaar, R.P., Buurke, J.H., Veltink, P.H., and Reenalda, J. (2023). Peak Tibial Acceleration Should Not Be Used as Indicator of Tibial Bone Loading during Running. Sports Biomech., 1\u201318.","DOI":"10.1080\/14763141.2022.2164345"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Matijevich, E.S., Branscombe, L.M., Scott, L.R., and Zelik, K.E. (2019). Ground Reaction Force Metrics Are Not Strongly Correlated with Tibial Bone Load When Running across Speeds and Slopes: Implications for Science, Sport and Wearable Tech. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0210000"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1002\/pmrj.12275","article-title":"Tibial Acceleration Measured from Wearable Sensors Is Associated with Loading Rates in Injured Runners","volume":"12","author":"Tenforde","year":"2020","journal-title":"PMR"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.clinbiomech.2018.07.007","article-title":"Accelerations from Wearable Accelerometers Reflect Knee Loading during Running after Anterior Cruciate Ligament Reconstruction","volume":"58","author":"Havens","year":"2018","journal-title":"Clin. Biomech."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/j.jbiomech.2011.12.006","article-title":"Accelerometer Counts and Raw Acceleration Output in Relation to Mechanical Loading","volume":"45","author":"Rowlands","year":"2012","journal-title":"J. Biomech."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.jbiomech.2019.01.039","article-title":"Validity and Reliability of Peak Tibial Accelerations as Real-Time Measure of Impact Loading during over-Ground Rearfoot Running at Different Speeds","volume":"86","author":"Six","year":"2019","journal-title":"J. Biomech."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1007\/s40279-019-01237-z","article-title":"Is Motorized Treadmill Running Biomechanically Comparable to Overground Running? A Systematic Review and Meta-Analysis of Cross-Over Studies","volume":"50","author":"Fuller","year":"2020","journal-title":"Sports Med."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Seminati, E., Nardello, F., Zamparo, P., Ardig\u00f2, L.P., Faccioli, N., and Minetti, A.E. (2013). Anatomically Asymmetrical Runners Move More Asymmetrically at the Same Metabolic Cost. 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