{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T09:37:27Z","timestamp":1787996247863,"version":"build-2784847793"},"reference-count":52,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:00:00Z","timestamp":1747180800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["52275498"],"award-info":[{"award-number":["52275498"]}]},{"name":"National Natural Science Foundation of China","award":["52175474"],"award-info":[{"award-number":["52175474"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Tension-free hernioplasty has effectively reduced postoperative recurrence and mitigated complications by employing polymer patches. However, clinically used polymer patches often fall short in terms of the anti-deformation, anti-adhesion, and tissue integration functions, which can result in visceral adhesions and foreign body reactions after implantation. In this study, a Janus three-layer composite patch was developed for abdominal wall defect repair using a combination of 3D printing, electrospraying, and electrospinning technologies. On the visceral side, a dense electrospun polyvinyl alcohol\/sodium hyaluronate (PVA\/HA) scaffold was fabricated to inhibit cell adhesion. The middle layer, composed of polycaprolactone (PCL), provided mechanical support. On the muscle-facing side, a loose and porous electrospun nanofiber scaffold was created through electrospraying and electrospinning, promoting cell adhesion and migration to facilitate tissue regeneration. Mechanical testing demonstrated that the composite patch possessed excellent tensile strength (23.58 N\/cm), surpassing the clinical standard (16 N\/cm). Both in vitro and in vivo evaluations confirmed the patch\u2019s outstanding biocompatibility. Compared with the control PCL patch, the Janus composite patch significantly reduced the visceral adhesion and enhanced the tissue repair in animal models. Collectively, this Janus composite patch integrated anti-deformation, anti-adhesion, and tissue-regenerative properties, providing a promising solution for effective abdominal wall defect repair.<\/jats:p>","DOI":"10.3390\/bioengineering12050522","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T12:22:17Z","timestamp":1747225337000},"page":"522","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Design of a Janus Composite Patch with Anti-Adhesive and Growth-Promoting Functions for Abdominal Wall Defect Repair"],"prefix":"10.3390","volume":"12","author":[{"given":"Qingxi","family":"Hu","sequence":"first","affiliation":[{"name":"Rapid Manufacturing Engineering Center, School of Mechatronical Engineering and Automation, Shanghai University, Shanghai 200444, China"},{"name":"National Demonstration Center for Experimental Engineering Training Education, Shanghai University, Shanghai 200444, China"},{"name":"Shanghai Key Laboratory of Intelligent Manufacturing and Robotics, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyang","family":"Hou","sequence":"additional","affiliation":[{"name":"Rapid Manufacturing Engineering Center, School of Mechatronical Engineering and Automation, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hekai","family":"Shi","sequence":"additional","affiliation":[{"name":"Shanghai Medical College of Fudan University, Fudan University, Shanghai 200032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongteng","family":"Song","sequence":"additional","affiliation":[{"name":"Rapid Manufacturing Engineering Center, School of Mechatronical Engineering and Automation, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Zhou","sequence":"additional","affiliation":[{"name":"Rapid Manufacturing Engineering Center, School of Mechatronical Engineering and Automation, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinli","family":"Hu","sequence":"additional","affiliation":[{"name":"Rapid Manufacturing Engineering Center, School of Mechatronical Engineering and Automation, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9243-1147","authenticated-orcid":false,"given":"Haiguang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Rapid Manufacturing Engineering Center, School of Mechatronical Engineering and Automation, Shanghai University, Shanghai 200444, China"},{"name":"National Demonstration Center for Experimental Engineering Training Education, Shanghai University, Shanghai 200444, China"},{"name":"Shanghai Key Laboratory of Intelligent Manufacturing and Robotics, Shanghai University, Shanghai 200444, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Gu","sequence":"additional","affiliation":[{"name":"Shanghai Medical College of Fudan University, Fudan University, Shanghai 200032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,14]]},"reference":[{"key":"ref_1","first-page":"2","article-title":"Design strategies and applications of biomaterials and devices for hernia repair","volume":"1","author":"Kalaba","year":"2016","journal-title":"Bioact. Mater."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s10029-009-0587-x","article-title":"Hernia repair: The search for ideal meshes","volume":"14","author":"Bringman","year":"2010","journal-title":"Hernia"},{"key":"ref_3","unstructured":"Burger, J. Chapter 7: Possible Gains of Optimizing Surgical Techniques in Preventing Incisional Hernia. Incisional Hernia Etiology, Prevention, Treatment, Erasmus University Rotterdam."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1016\/S0140-6736(18)30298-8","article-title":"Mesh versus suture repair of umbilical hernia in adults: A randomised, double-blind, controlled, multicentre trial","volume":"391","author":"Kaufmann","year":"2018","journal-title":"Lancet"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Costa, A., Adamo, S., Gossetti, F., D\u2019Amore, L., Ceci, F., Negro, P., and Bruzzone, P. (2019). Biological scaffolds for abdominal wall repair: Future in clinical application?. Materials, 12.","DOI":"10.3390\/ma12152375"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"10054","DOI":"10.1039\/D4TB01543F","article-title":"3D printing\/electrospinning of a bilayered composite patch with antibacterial and antiadhesive properties for repairing abdominal wall defects","volume":"12","author":"Hu","year":"2024","journal-title":"J. Mater. Chem. B"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1002\/jbm.b.33586","article-title":"Synthetic surgical meshes used in abdominal wall surgery: Part I\u2014Materials and structural conformation","volume":"105","author":"Todros","year":"2017","journal-title":"J. Biomed. Mater. Res. Part B Appl. Biomater."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s00192-016-3029-1","article-title":"In vivo response to polypropylene following implantation in animal models: A review of biocompatibility","volume":"28","author":"Kelly","year":"2017","journal-title":"Int. Urogynecol. J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2105614","DOI":"10.1002\/adfm.202105614","article-title":"Bio-multifunctional hydrogel patches for repairing full-thickness abdominal wall defects","volume":"31","author":"Yin","year":"2021","journal-title":"Adv. Funct. Mater."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1007\/s00464-005-0706-4","article-title":"Evaluation of new prosthetic meshes for ventral hernia repair","volume":"20","author":"Burger","year":"2006","journal-title":"Surg. Endosc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2108992","DOI":"10.1002\/adma.202108992","article-title":"Peritoneum-inspired Janus porous hydrogel with anti-deformation, anti-adhesion, and pro-healing characteristics for abdominal wall defect treatment","volume":"34","author":"Liang","year":"2022","journal-title":"Adv. Mater."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"119577","DOI":"10.1016\/j.carbpol.2022.119577","article-title":"Study on repair of abdominal wall defect rats with hernia mesh coated with chitosan-based photosensitive hydrogel","volume":"291","author":"Hu","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1038\/nature24660","article-title":"Orthogonal muscle fibres have different instructive roles in planarian regeneration","volume":"551","author":"Scimone","year":"2017","journal-title":"Nature"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"152764","DOI":"10.1016\/j.cej.2024.152764","article-title":"Endogenous stimulation-driven Janus mesh with antibacterial, anti-adhesive and prohealing performances for hernia repair","volume":"494","author":"Sun","year":"2024","journal-title":"Chem. Eng. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.msec.2018.05.068","article-title":"Fabrication and investigation of silica nanofibers via electrospinning","volume":"91","author":"Shahhosseininia","year":"2018","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Hollander, A.P., and Hatton, P.V. (2004). Biopolymer Methods in Tissue Engineering, Springer.","DOI":"10.1385\/159259428X"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Mao, Y., Guidoin, R., Brochu, G., Li, Y., Zhang, Z., Wang, F., and Wang, L. (2021). Facile fabrication of phospholipid-functionalized nanofiber-based barriers with enhanced anti-adhesion efficiency. Colloids Surf. B Biointerfaces, 203.","DOI":"10.1016\/j.colsurfb.2021.111728"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1002\/jbm.a.32609","article-title":"The topographical effect of electrospun nanofibrous scaffolds on the in vivo and in vitro foreign body reaction","volume":"93","author":"Cao","year":"2010","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"111930","DOI":"10.1016\/j.msec.2021.111930","article-title":"Development of electrospun core\u2013shell polymeric mat using poly (ethyl-2) cyanoacrylate\/polyurethane to attenuate biological adhesion on polymeric mesh implants","volume":"122","author":"Chalony","year":"2021","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"6573","DOI":"10.1039\/D3BM00786C","article-title":"An anti-inflammatory chondroitin sulfate-poly (lactic-co-glycolic acid) composite electrospinning membrane for postoperative abdominal adhesion prevention","volume":"11","author":"Gao","year":"2023","journal-title":"Biomater. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.msec.2010.02.001","article-title":"Utilizing NaCl to increase the porosity of electrospun materials","volume":"31","author":"Wright","year":"2011","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1002\/mabi.201200318","article-title":"Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures","volume":"13","author":"Li","year":"2013","journal-title":"Macromol. Biosci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.compositesa.2016.09.015","article-title":"Investigation of microporous composite scaffolds fabricated by embedding sacrificial polyethylene glycol microspheres in nanofibrous membrane","volume":"91","author":"Gao","year":"2016","journal-title":"Compos. Part A Appl. Sci. Manuf."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Song, Y., Hu, Q., Liu, S., Wang, Y., Zhang, H., Chen, J., and Yao, G. (2024). Electrospinning\/3D printing drug-loaded antibacterial polycaprolactone nanofiber\/sodium alginate-gelatin hydrogel bilayer scaffold for skin wound repair. Int. J. Biol. Macromol., 275.","DOI":"10.1016\/j.ijbiomac.2024.129705"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.bmt.2024.10.001","article-title":"Electrospinning drug-loaded polycaprolactone\/polycaprolactone-gelatin multi-functional bilayer nanofibers composite scaffold for postoperative wound healing of cutaneous squamous cell carcinoma","volume":"8","author":"Song","year":"2024","journal-title":"Biomed. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"129038","DOI":"10.1016\/j.matlet.2020.129038","article-title":"Synthesis and characterization of anti-adhesion tricomposite electrospun nanofiber barrier membrane for use in post-surgical adhesion conditions","volume":"285","author":"Sathish","year":"2021","journal-title":"Mater. Lett."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.carbpol.2007.09.018","article-title":"Intermolecular interactions in electrospun collagen\u2013chitosan complex nanofibers","volume":"72","author":"Chen","year":"2008","journal-title":"Carbohydr. Polym."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3501","DOI":"10.2147\/DDDT.S103824","article-title":"Hyaluronic acid and oxidized regenerated cellulose prevent adhesion reformation after adhesiolysis in rat models","volume":"10","author":"Zhang","year":"2016","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"80","DOI":"10.3109\/08941939.2015.1076911","article-title":"Histological analysis of intra-abdominal adhesions treated with sodium hyaluronate and carboxymethylcellulose gel","volume":"29","author":"Jurado","year":"2016","journal-title":"J. Investig. Surg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.morpho.2020.05.002","article-title":"Biomaterials preparation by electrospinning of gelatin and sodium hyaluronate\/gelatin nanofibers with non-toxic solvents","volume":"104","author":"Arbez","year":"2020","journal-title":"Morphologie"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"120172","DOI":"10.1016\/j.ijpharm.2020.120172","article-title":"Electrospun, sepiolite-loaded poly (vinyl alcohol)\/soy protein isolate nanofibers: Preparation, characterization, and their drug release behavior","volume":"594","author":"Gutschmidt","year":"2021","journal-title":"Int. J. Pharm."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.msec.2018.09.033","article-title":"Development and evaluation of antibacterial electrospun pea protein isolate-polyvinyl alcohol nanocomposite mats incorporated with cinnamaldehyde","volume":"94","author":"Maftoonazad","year":"2019","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Hu, Q., Wu, J., Zhang, H., Dong, W., Gu, Y., and Liu, S. (2022). Designing Double-Layer Multimaterial Composite Patch Scaffold with Adhesion Resistance for Hernia Repair. Macromol. Biosci., 22.","DOI":"10.1002\/mabi.202100510"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1959","DOI":"10.1080\/19443994.2014.981864","article-title":"Electrospun nanofibrous composite membranes of chitosan\/polyvinyl alcohol-polyacrylonitrile: Preparation, characterization, and performance","volume":"57","author":"Hejazi","year":"2016","journal-title":"Desalination Water Treat."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1098\/rsif.2005.0039","article-title":"Statistical geometry of pores and statistics of porous nanofibrous assemblies","volume":"2","author":"Eichhorn","year":"2005","journal-title":"J. R. Soc. Interface"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"153662","DOI":"10.1016\/j.cej.2024.153662","article-title":"3D printed biomimetic composite scaffolds with sequential releasing of copper ions and dexamethasone for cascade regulation of angiogenesis and osteogenesis","volume":"496","author":"Song","year":"2024","journal-title":"Chem. Eng. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1111\/j.1747-1567.2009.00597.x","article-title":"3D hybrid ballistic fabric testing using a 3d digital image correlation system","volume":"35","author":"Nader","year":"2011","journal-title":"Exp. Tech."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/j.jmbbm.2017.08.008","article-title":"Biomechanical and histologic evaluation of two application forms of surgical glue for mesh fixation to the abdominal wall","volume":"75","author":"Pascual","year":"2017","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.ijpharm.2019.04.073","article-title":"Fabrication of doxycycline-loaded electrospun PCL\/PEO membranes for a potential drug delivery system","volume":"565","author":"Bulbul","year":"2019","journal-title":"Int. J. Pharm."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.carbpol.2018.03.075","article-title":"Levan based fibrous scaffolds electrospun via co-axial and single-needle techniques for tissue engineering applications","volume":"193","author":"Avsar","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/j.ijbiomac.2020.07.126","article-title":"Electrospun PVA\/hyaluronic acid\/L-arginine nanofibers for wound healing applications: Nanofibers optimization and in vitro bioevaluation","volume":"164","author":"Hussein","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1002\/adv.21820","article-title":"Hydrophilic films: How hydrophilicity affects blood compatibility and cellular compatibility","volume":"37","author":"Kuo","year":"2018","journal-title":"Adv. Polym. Technol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2753","DOI":"10.1039\/C5TB00129C","article-title":"A biocompatible hydrogel with improved stiffness and hydrophilicity for modular tissue engineering assembly","volume":"3","author":"Wei","year":"2015","journal-title":"J. Mater. Chem. B"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Antonov, D.V., Islamova, A.G., and Strizhak, P.A. (2023). Hydrophilic and hydrophobic surfaces: Features of interaction with liquid drops. Materials, 16.","DOI":"10.3390\/ma16175932"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Xu, W., Ma, P., Jiang, G., and Wan, A. (2018). Mechanical properties of polypropylene warp-knitted hernia repair mesh with different pull densities. Polymers, 10.","DOI":"10.3390\/polym10121322"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.jmbbm.2017.05.008","article-title":"Mechanical properties of the abdominal wall and biomaterials utilized for hernia repair","volume":"74","author":"Deeken","year":"2017","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Wurth, J.J., Blumenthal, N.R., and Shastri, V.P. (2014). Hydrophilization of poly (caprolactone) copolymers through introduction of oligo (ethylene glycol) moieties. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0099157"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1002\/jbm.a.36326","article-title":"Novel large-pore lightweight polypropylene mesh has better biocompatibility for rat model of hernia","volume":"106","author":"Qiu","year":"2018","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1080\/08941939.2022.2087240","article-title":"Collagen deposition and inflammatory response associated with macroporous mesh shrinkage in incisional hernia repair: A rat model","volume":"35","author":"Tanprasert","year":"2022","journal-title":"J. Investig. Surg."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Nikfarjam, S., Aldubaisi, Y., Swami, V., Swami, V., Xu, G., Vaughan, M.B., Wolf, R.F., and Khandaker, M. (2023). Polycaprolactone electrospun nanofiber membrane with skin graft containing collagen and bandage containing MgO nanoparticles for wound healing applications. Polymers, 15.","DOI":"10.3390\/polym15092014"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1007\/s10753-007-9043-1","article-title":"The effects of anti-adhesion materials in preventing postoperative adhesion in abdominal cavity (anti-adhesion materials for postoperative adhesions)","volume":"30","author":"Sahin","year":"2007","journal-title":"Inflammation"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"21","DOI":"10.15574\/SP.2022.122.21","article-title":"Clinical effectiveness of sodium hyaluronate gel usage for prevention of postoperative adhesion in children","volume":"2","author":"Kvashnina","year":"2022","journal-title":"Mod. Pediatr. Ukr."}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/12\/5\/522\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:32:48Z","timestamp":1760031168000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/12\/5\/522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,14]]},"references-count":52,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["bioengineering12050522"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering12050522","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,14]]}}}