{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:31:55Z","timestamp":1789468315799,"version":"build-2803163510"},"reference-count":59,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bauerfeind AG"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>The knee is one of the most frequently injured joints, involving various structures. To prevent reinjury after rehabilitation, braces are commonly used. However, most studies on knee supports focus on subjects with anterior cruciate ligament (ACL) injuries and do not account for muscle fatigue, which typically occurs during prolonged intense training and can significantly increase the risk of injury. Hence, this study investigates the acute effects of wearing a knee brace on biomechanics in subjects with a history of various unilateral knee injuries or pain under muscle fatigue. In total, 50 subjects completed an intense fatigue protocol and then performed counter-movement jumps and running tests on a force plate while tracking kinematics with a marker-based 3D motion analysis system. Additionally, subjects filled out a visual analog scale (VAS) to assess knee pain and stability. Tests were conducted on the injured leg with and without a knee brace (Sports Knee Support, Bauerfeind AG, Zeulenroda-Triebes, Germany) and on the healthy leg. Results indicated that wearing the knee brace stabilized knee movement in the frontal plane, with a significant reduction in maximal medio-lateral knee acceleration and knee abduction moment during running and jumping. The brace also normalized loading on the injured leg. We observed higher maximal knee flexion moments, which were associated with increased vertical ground reaction forces, segment velocities, and knee flexion angles. Subjects reported less pain and greater stability while wearing the knee brace. Therefore, we confirm that wearing a knee brace on the injured leg improves joint biomechanics by enhancing stability and kinematics and reducing pain during running and jumping, even with muscle fatigue. Consequently, wearing a knee brace after a knee joint injury may reduce the risk of reinjury.<\/jats:p>","DOI":"10.3390\/bioengineering12060661","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T10:47:22Z","timestamp":1750070842000},"page":"661","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Running and Jumping After Muscle Fatigue in Subjects with a History of Knee Injury: What Are the Acute Effects of Wearing a Knee Brace on Biomechanics?"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0805-2350","authenticated-orcid":false,"given":"Tobias","family":"He\u00df","sequence":"first","affiliation":[{"name":"Department of Movement Science for Prevention and Rehabilitation, Institute of Human Movement Science and Health, Chemnitz University of Technology, Th\u00fcringer Weg 11, 09126 Chemnitz, Germany"},{"name":"Research Group for Biomechanics and Sensory Function, Institute of Human Movement Science and Health, Chemnitz University of Technology, Th\u00fcringer Weg 11, 09126 Chemnitz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4828-8624","authenticated-orcid":false,"given":"Thomas L.","family":"Milani","sequence":"additional","affiliation":[{"name":"Research Group for Biomechanics and Sensory Function, Institute of Human Movement Science and Health, Chemnitz University of Technology, Th\u00fcringer Weg 11, 09126 Chemnitz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Stoll","sequence":"additional","affiliation":[{"name":"Department of Movement Science for Prevention and Rehabilitation, Institute of Human Movement Science and Health, Chemnitz University of Technology, Th\u00fcringer Weg 11, 09126 Chemnitz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7662-8062","authenticated-orcid":false,"given":"Christian","family":"Mitschke","sequence":"additional","affiliation":[{"name":"Department of Movement Science for Prevention and Rehabilitation, Institute of Human Movement Science and Health, Chemnitz University of Technology, Th\u00fcringer Weg 11, 09126 Chemnitz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1007\/s00264-023-06084-2","article-title":"Risk factors of knee reinjury after anterior cruciate ligament reconstruction","volume":"48","author":"Adhitya","year":"2024","journal-title":"Int. Orthop."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"411","DOI":"10.2165\/00007256-200636050-00004","article-title":"A risk-factor model for anterior cruciate ligament injury","volume":"36","author":"Hughes","year":"2006","journal-title":"Sports Med."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Butler, L., Olander, H.L., Erdman, A., and Ulman, S. (2025). Relationship of knee abduction moment to lower extremity segment accelerations during sport-specific movements in youth anterior cruciate ligament reconstruction patients at return-to-play. Clin. Biomech., 124.","DOI":"10.1016\/j.clinbiomech.2025.106485"},{"key":"ref_4","first-page":"728","article-title":"Mechanism and Prevention of Acute Knee Joint Injury During Exercise","volume":"7","author":"Hou","year":"2024","journal-title":"Trans. Mater. Biotechnol. Life Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1016\/j.jsams.2022.08.016","article-title":"Epidemiology of gradual-onset knee injuries in team ball-sports: A systematic review with meta-analysis of prevalence, incidence, and burden by sex, sport, age, and participation level","volume":"25","author":"Chia","year":"2022","journal-title":"J. Sci. Med. Sport"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1186\/s13018-020-01638-1","article-title":"Prevalence of knee injuries among male college students in Riyadh, Kingdom of Saudi Arabia","volume":"15","author":"Almaawi","year":"2020","journal-title":"J. Orthop. Surg. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1590\/1413-78522014220300475","article-title":"Common injuries in athletes\u2019 knee: Experience of a specialized center","volume":"22","author":"Nicolini","year":"2014","journal-title":"Acta Ortop. Bras."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.knee.2006.01.005","article-title":"Epidemiology of athletic knee injuries: A 10-year study","volume":"13","author":"Majewski","year":"2006","journal-title":"Knee"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1136\/bjsm.34.3.227-a","article-title":"Epidemiology of knee injuries: Diagnosis and triage","volume":"34","author":"Bollen","year":"2000","journal-title":"Br. J. Sports Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1177\/0363546506286866","article-title":"Understanding and preventing noncontact anterior cruciate ligament injuries: A review of the Hunt Valley II meeting, January 2005","volume":"34","author":"Griffin","year":"2006","journal-title":"Am. J. Sports Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1177\/1941738120912846","article-title":"One in 5 Athletes Sustain Reinjury Upon Return to High-Risk Sports After ACL Reconstruction: A Systematic Review in 1239 Athletes Younger Than 20 Years","volume":"12","author":"Noyes","year":"2020","journal-title":"Sports Health"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1002\/ksa.12473","article-title":"More knee reinjuries after ACL reconstruction compared to nonsurgical treatment of the ACL. A 2-year follow-up of the NACOX prospective cohort study","volume":"33","author":"Selin","year":"2025","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"F\u00e4ltstr\u00f6m, A., Asker, M., Weiss, N., Lyberg, V., Wald\u00e9n, M., H\u00e4gglund, M., Tranaeus, U., and Skillgate, E. (2024). Poor knee strength is associated with higher incidence of knee injury in adolescent female football players: The Karolinska football injury cohort. Knee Surg. Sports Traumatol. Arthrosc., ahead of print.","DOI":"10.1002\/ksa.12567"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1002\/ksa.12135","article-title":"Functional knee brace use for 21 h leads to a longer duration to achieve peak vertical ground reaction forces and the removal of the brace after 17.5 h results in faster loading of the knee joint","volume":"32","author":"Rishiraj","year":"2024","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"42","DOI":"10.36082\/jpost.v3i2.1945","article-title":"Effect of Soft Knee Brace on Shank Movement in Running","volume":"3","author":"Kitagawa","year":"2024","journal-title":"J. Prosthet. Orthot. Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Mougin, F., Begon, M., Desmyttere, G., Bleau, J., Nault, M.-L., and Cherni, Y. (2024). Knee Biomechanics during Walking in Individuals with Anterior Cruciate Ligament Repair: The Role of a Custom 3D Printed Knee Brace. bioRxiv, preprints.","DOI":"10.1101\/2024.07.04.601890"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1016\/j.knee.2018.07.017","article-title":"Effect of wearing a knee brace or sleeve on the knee joint and anterior cruciate ligament force during drop jumps: A clinical intervention study","volume":"25","author":"Moon","year":"2018","journal-title":"Knee"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1177\/0363546503262204","article-title":"Immediate effects of a knee brace with a constraint to knee extension on knee kinematics and ground reaction forces in a stop-jump task","volume":"32","author":"Yu","year":"2004","journal-title":"Am. J. Sports Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3526","DOI":"10.1080\/14763141.2024.2409656","article-title":"The effect of knee braces on knee joint kinematics at different exercise speeds","volume":"23","author":"Wang","year":"2024","journal-title":"Sports Biomech."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.ptsp.2016.08.005","article-title":"Effects of prophylactic knee bracing on knee joint kinetics and kinematics during netball specific movements","volume":"23","author":"Sinclair","year":"2017","journal-title":"Phys. Ther. Sport"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2919","DOI":"10.1002\/ksa.12312","article-title":"Knee braces and foot orthoses multimodal 3-month treatment of medial knee osteoarthritis in a randomised crossover trial","volume":"32","author":"Dessery","year":"2024","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e13116","DOI":"10.1016\/j.heliyon.2023.e13116","article-title":"Kinematic study of the overall unloading brace for the knee","volume":"9","author":"Guo","year":"2023","journal-title":"Heliyon"},{"key":"ref_23","first-page":"81","article-title":"Effect of using of brace in three different knee flexion angles on running economy and lower limb muscular co-contraction in individuals with genu varus","volume":"23","author":"Norinasab","year":"2021","journal-title":"J. Arak Univ. Med. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Siebers, H.L., Eschweiler, J., Pinz, J., Tingart, M., and Rath, B. (2020). The effect of a knee brace in dynamic motion-An instrumented gait analysis. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0238722"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Mortaza, N., Ebrahimi, I., Jamshidi, A.A., Abdollah, V., Kamali, M., Abas, W.A.B.W., and Osman, N.A.A. (2012). The effects of a prophylactic knee brace and two neoprene knee sleeves on the performance of healthy athletes: A crossover randomized controlled trial. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0050110"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1007\/s00167-011-1491-3","article-title":"The effect of prophylactic knee bracing on performance: Balance, proprioception, coordination, and muscular power","volume":"19","author":"Baltaci","year":"2011","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"105","DOI":"10.47447\/tjsm.0813","article-title":"The effects of knee brace use on landing error, balance, and crossover hop test in healthy athletes","volume":"59","author":"Arslan","year":"2024","journal-title":"Turk. J. Sports Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1177\/0363546516637178","article-title":"Effects of Prophylactic Knee Bracing on Lower Limb Kinematics, Kinetics, and Energetics During Double-Leg Drop Landing at 2 Heights","volume":"44","author":"Ewing","year":"2016","journal-title":"Am. J. Sports Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1007\/s00402-024-05206-8","article-title":"Does an early post traumatic knee brace reduce the incidence of knee rotational instability?","volume":"144","author":"Murgier","year":"2024","journal-title":"Arch. Orthop. Trauma Surg."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Focke, A., Steingrebe, H., M\u00f6hler, F., Ringhof, S., Sell, S., Potthast, W., and Stein, T. (2020). Effect of Different Knee Braces in ACL-Deficient Patients. Front. Bioeng. Biotechnol., 8.","DOI":"10.3389\/fbioe.2020.00964"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1016\/j.otsr.2019.04.015","article-title":"The effect of knee bracing on the knee function and stability following anterior cruciate ligament reconstruction: A systematic review and meta-analysis of randomized controlled trials","volume":"105","author":"Yang","year":"2019","journal-title":"Orthop. Traumatol. Surg. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s00167-006-0072-3","article-title":"Study on three-dimensional kinematics and electromyography of ACL deficient knee participants wearing a functional knee brace during running","volume":"14","author":"Lamontagne","year":"2006","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1177\/1941738114524910","article-title":"Changes in landing mechanics in patients following anterior cruciate ligament reconstruction when wearing an extension constraint knee brace","volume":"6","author":"Butler","year":"2014","journal-title":"Sports Health"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2325967120944255","DOI":"10.1177\/2325967120944255","article-title":"Physical Performance Improves With Time and a Functional Knee Brace in Athletes After ACL Reconstruction","volume":"8","author":"Dickerson","year":"2020","journal-title":"Orthop. J. Sports Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1055\/s-0032-1304634","article-title":"Effect of brace design on patients with ACL-ruptures","volume":"33","author":"Strutzenberger","year":"2012","journal-title":"Int. J. Sports Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1177\/03635465241233161","article-title":"Rehabilitation and Return to Play Protocols After Anterior Cruciate Ligament Reconstruction in Soccer Players: A Systematic Review","volume":"53","author":"Mayer","year":"2025","journal-title":"Am. J. Sports Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2325967120981641","DOI":"10.1177\/2325967120981641","article-title":"Effect of Prophylactic Knee Bracing on Anterior Cruciate Ligament Agonist and Antagonist Muscle Forces During Perturbed Walking","volume":"9","author":"Haddara","year":"2021","journal-title":"Orthop. J. Sports Med."},{"key":"ref_38","first-page":"159","article-title":"The effects of prophylactic knee bracing on running gait","volume":"30","author":"Liggett","year":"1995","journal-title":"J. Athl. Train."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2088","DOI":"10.1080\/02640414.2024.2312481","article-title":"Physiological, perceptual, and biomechanical differences between treadmill and overground walking in healthy adults: A systematic review and meta-analysis","volume":"41","author":"Bonanno","year":"2023","journal-title":"J. Sports Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.gaitpost.2021.11.009","article-title":"Is treadmill walking biomechanically comparable to overground walking? A systematic review","volume":"92","author":"Semaan","year":"2022","journal-title":"Gait Posture"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1152\/japplphysiol.01380.2006","article-title":"Biomechanics of overground vs. treadmill walking in healthy individuals","volume":"104","author":"Lee","year":"2008","journal-title":"J. Appl. Physiol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Hill, M., Kiesewetter, P., Milani, T.L., and Mitschke, C. (2024). An Investigation of Running Kinematics with Recovered Anterior Cruciate Ligament Reconstruction on a Treadmill and In-Field Using Inertial Measurement Units: A Preliminary Study. Bioengineering, 11.","DOI":"10.3390\/bioengineering11040404"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1080\/17461391.2011.568632","article-title":"Quantification of the manifestations of fatigue during treadmill running","volume":"12","author":"Stirling","year":"2012","journal-title":"Eur. J. Sport Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Koh, J., Zaffagnini, S., Kuroda, R., Longo, U.G., and Amirouche, F. (2021). Anatomy and Biomechanics of the Collateral Ligaments of the Knee. Orthopaedic Biomechanics in Sports Medicine, Springer International Publishing.","DOI":"10.1007\/978-3-030-81549-3"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1097\/JSA.0000000000000279","article-title":"Knee Ligament Anatomy and Biomechanics","volume":"28","author":"Hassebrock","year":"2020","journal-title":"Sports Med. Arthrosc. Rev."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1177\/03635465990270042301","article-title":"Biomechanics of knee ligaments","volume":"27","author":"Woo","year":"1999","journal-title":"Am. J. Sports Med."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Dashti Rostami, K., Alizadeh, M.H., Minoonejad, H., Yazdi, H., and Thomas, A. (2018). Effect of Fatigue on Electromyographic Activity Patterns of the Knee Joint Muscles in Anterior Cruciate Ligament Reconstructed and Deficient Patients during Landing Task. J. Funct. Morphol. Kinesiol., 3.","DOI":"10.3390\/jfmk3020022"},{"key":"ref_48","first-page":"285","article-title":"Clinical Strategies for Addressing Muscle Weakness Following Knee Injury","volume":"34","author":"Pietrosimone","year":"2015","journal-title":"Sports Rehabil."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"610","DOI":"10.4085\/1062-6050-48.3.23","article-title":"Lower extremity muscle strength after anterior cruciate ligament injury and reconstruction","volume":"48","author":"Thomas","year":"2013","journal-title":"J. Athl. Train."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.gaitpost.2018.10.026","article-title":"The effect of proprioceptive knee bracing on knee stability after anterior cruciate ligament reconstruction","volume":"67","author":"Richards","year":"2019","journal-title":"Gait Posture"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"11","DOI":"10.2519\/jospt.2002.32.1.11","article-title":"The Effect of Bracing on Proprioception of Knees with Anterior Cruciate Ligament Injury","volume":"32","author":"Beynnon","year":"2002","journal-title":"J. Orthop. Sports Phys. Ther."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/BF01577413","article-title":"Proprioception and joint stability","volume":"4","author":"Jerosch","year":"1996","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1016\/0959-4388(95)80107-3","article-title":"Proprioceptive regulation of locomotion","volume":"5","author":"Pearson","year":"1995","journal-title":"Curr. Opin. Neurobiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.ptsp.2024.11.005","article-title":"Knee proprioception four months after anterior cruciate ligament reconstruction: Impact of limb dominance, anterolateral procedure, and association with readiness to return to sport","volume":"71","author":"Drigny","year":"2025","journal-title":"Phys. Ther. Sport"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"294","DOI":"10.4103\/0019-5413.80320","article-title":"Proprioception in anterior cruciate ligament deficient knees and its relevance in anterior cruciate ligament reconstruction","volume":"45","author":"Dhillon","year":"2011","journal-title":"Indian J. Orthop."},{"key":"ref_56","first-page":"1168","article-title":"The Effect of Using Brace in Three Different Knee Flexion Angles on Frequency of Muscular Activity During Running in People With Genu Varus","volume":"10","author":"Norinasab","year":"2022","journal-title":"Sarem J. Reprod. Med."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Huang, T., Ruan, M., Huang, S., Fan, L., and Wu, X. (2024). Comparison of kinematics and joint moments calculations for lower limbs during gait using markerless and marker-based motion capture. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1280363"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Camomilla, V., Cereatti, A., Cutti, A.G., Fantozzi, S., Stagni, R., and Vannozzi, G. (2017). Methodological factors affecting joint moments estimation in clinical gait analysis: A systematic review. Biomed. Eng. Online, 16.","DOI":"10.1186\/s12938-017-0396-x"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Cleather, D.J., and Bull, A.M.J. (2010). Influence of inverse dynamics methods on the calculation of inter-segmental moments in vertical jumping and weightlifting. Biomed. Eng. Online, 9.","DOI":"10.1186\/1475-925X-9-74"}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/12\/6\/661\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:52:59Z","timestamp":1760032379000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/12\/6\/661"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,16]]},"references-count":59,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["bioengineering12060661"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering12060661","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,16]]}}}