{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:34:01Z","timestamp":1787027641242,"version":"build-2736575974"},"reference-count":47,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["24K19928"],"award-info":[{"award-number":["24K19928"]}]},{"name":"Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["24K19886"],"award-info":[{"award-number":["24K19886"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>This study investigated the effects of local fibroblast growth factor (FGF)-2 application on periodontal healing in an osteoporotic model, both in vivo and in vitro. Wistar rats were divided into the ovariectomy (OVX) and Control groups. Periodontal defects were created 8 weeks post-OVX and treated with hydroxypropylcellulose (HPC) or FGF-2 + HPC. Healing was evaluated through micro-computed tomography and histological analyses at 2 and 4 weeks. In vitro, bone marrow mesenchymal stromal cells (BMSCs) were cultured with\/without FGF-2 and assessed for cell morphology, viability\/proliferation, and osteoblastic marker expression. Alkaline phosphatase (ALP) staining was also performed. FGF-2-treated defects in both groups showed significantly greater bone volume fraction, trabecular number, and thickness compared to HPC only. Histologically, FGF-2 enhanced new bone formation, with the greatest levels in the Control group. In vitro, OVX BMSCs showed reduced actin staining versus controls. FGF-2 increased cell viability\/proliferation and protrusions in both groups while downregulating Alpl and Bglap expression levels and reducing ALP-positive cells. FGF-2 increased new bone formation in the OVX group, stimulated proliferation of OVX BMSCs, and modulated their differentiation. FGF-2 could enhance periodontal healing even under osteoporotic conditions, albeit to a lesser extent.<\/jats:p>","DOI":"10.3390\/bioengineering12070748","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T13:44:19Z","timestamp":1752241459000},"page":"748","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Therapeutic Potential of Local Application of Fibroblast Growth Factor-2 to Periodontal Defects in a Preclinical Osteoporosis Model"],"prefix":"10.3390","volume":"12","author":[{"given":"Shinta","family":"Mori","sequence":"first","affiliation":[{"name":"Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sho","family":"Mano","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan"},{"name":"Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Miyata","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tasuku","family":"Murakami","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wataru","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6428-3677","authenticated-orcid":false,"given":"Kentaro","family":"Imamura","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan"},{"name":"Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0065-2207","authenticated-orcid":false,"given":"Atsushi","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan"},{"name":"Oral Health Science Center, Tokyo Dental College, Chiyoda-ku, Tokyo 1010061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1186\/s13018-021-02772-0","article-title":"The global prevalence of osteoporosis in the world: A comprehensive systematic review and meta-analysis","volume":"16","author":"Salari","year":"2021","journal-title":"J. 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