{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:31:10Z","timestamp":1760059870215,"version":"build-2065373602"},"reference-count":70,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Glaucoma is a trans-synaptic neurodegenerative disease, and the pathological increase in intraocular pressure (IOP) is a major risk factor of glaucoma. High IOP alters microstructure and morphologies of the brain tissue. Since mechanical properties of the brain are sensitive to the alteration of the tissue microstructure, we investigate how varying durations of chronic elevated IOP alter brain mechanical properties. A chronic high IOP rat model was induced by episcleral vein cauterization with subconjunctival injection of 5-Fluorouracil. At 2, 4 and 8 weeks after induction, indentation tests were performed on the brain slices to measure mechanical properties in the hippocampus, lateral geniculate nucleus and occipital lobe of both hemispheres. Meanwhile, the brain\u2019s microstructure was assessed via F-actin and myelin staining. Compared to the blank control group, the Young\u2019s modulus decreased in all three brain regions in the highIOP experimental groups. F-actin fluorescence intensity and myelin area fraction were reduced in the hippocampus, while \u03b2-amyloid levels and tau phosphorylation were elevated in the experimental groups. Our study provides insight into Alzheimer\u2019s disease pathogenesis by demonstrating how chronic high IOP alters the brain\u2019s mechanical properties.<\/jats:p>","DOI":"10.3390\/bioengineering12080787","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T11:44:45Z","timestamp":1753184685000},"page":"787","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Change in Mechanical Property of Rat Brain Suffering from Chronic High Intraocular Pressure"],"prefix":"10.3390","volume":"12","author":[{"given":"Yukai","family":"Zeng","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering, Capital Medical University, Beijing 100069, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunya","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Capital Medical University, Beijing 100069, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengyuan","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4267-8728","authenticated-orcid":false,"given":"Xiuqing","family":"Qian","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Capital Medical University, Beijing 100069, China"},{"name":"Beijing Key Laboratory of Intelligent Diagnosis Technology and Equipment for Optic Nerve-Related Eye Diseases, Beijing 100069, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/S0079-6123(08)01132-1","article-title":"Glaucoma of the brain: A disease model for the study of transsynaptic neural degeneration","volume":"173","author":"Yuecel","year":"2008","journal-title":"Prog. 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