{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:35:02Z","timestamp":1760060102001,"version":"build-2065373602"},"reference-count":72,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:00:00Z","timestamp":1753920000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>(1) Background: Chronic myeloid leukemia (CML) is a myeloproliferative disorder driven by the BCR::ABL oncoprotein. During the chronic phase, Philadelphia chromosome-positive hematopoietic stem cells generate proliferative myeloid cells with various stages of maturation. Despite this expansion, leukemic stem cells (LSCs) retain self-renewal capacity via asymmetric cell divisions, sustaining the stem cell pool. Quiescent LSCs are known to be resistant to tyrosine kinase inhibitors (TKIs), potentially through BCR::ABL-independent signaling pathways. We hypothesize that dysregulation of genes governing asymmetric division in LSCs contributes to disease progression, and that their expression pattern may serve as a prognostic marker during the chronic phase of CML. (2) Methods: Genes related to asymmetric cell division in the context of hematopoietic stem cells were extracted from the PubMed database with the keyword \u201casymmetric hematopoietic stem cell\u201d. The collected relative gene set was tested on two independent bulk transcriptome cohorts and the results were confirmed by single-cell RNA sequencing. (3) Results: The expression of genes involved in asymmetric hematopoietic stem cell division was found to discriminate disease phases during CML progression in the two independent transcriptome cohorts. Concordance between cohorts was observed on asymmetric molecules downregulated during blast crisis (BC) as compared to the chronic phase (CP). This downregulation during the BC phase was confirmed at single-cell level for SELL, CD63, NUMB, HK2, and LAMP2 genes. Single-cell analysis during the CP found that CD63 is associated with a poor prognosis phenotype, with the opposite prediction revealed by HK2 and NUMB expression. The single-cell trajectory reconstitution analysis in CP samples showed CD63 regulation highlighting a trajectory cluster implicating HSPB1, PIM2, ANXA5, LAMTOR1, CFL1, CD52, RAD52, MEIS1, and PDIA3, known to be implicated in hematopoietic malignancies. (4) Conclusion: Regulation of CD63, a tetraspanin involved in the asymmetric division of hematopoietic stem cells, was found to be associated with poor prognosis during CML progression and could be a potential new therapeutic target.<\/jats:p>","DOI":"10.3390\/bioengineering12080830","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T15:09:53Z","timestamp":1754492993000},"page":"830","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Regulation of Tetraspanin CD63 in Chronic Myeloid Leukemia (CML): Single-Cell Analysis of Asymmetric Hematopoietic Stem Cell Division Genes"],"prefix":"10.3390","volume":"12","author":[{"given":"Christophe","family":"Desterke","sequence":"first","affiliation":[{"name":"Faculty of Medicine, University Paris Saclay, INSERM UMRS-1310, 94800 Villejuif, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annelise","family":"Bennaceur-Griscelli","sequence":"additional","affiliation":[{"name":"INSERM UMR-S-1310, University Paris Saclay, 94800 Villejuif, France"},{"name":"Faculty of Medicine, University Paris Saclay, 94270 Le Kremlin Bicetre, France"},{"name":"INGESTEM National iPSC Infrastructure, 94800 Villejuif, France"},{"name":"CITHERA, Centre for IPSC Therapies, INSERM UMS-45, Genopole Campus, 91100 Evry, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4861-0137","authenticated-orcid":false,"given":"Ali G.","family":"Turhan","sequence":"additional","affiliation":[{"name":"INSERM UMR-S-1310, University Paris Saclay, 94800 Villejuif, France"},{"name":"Faculty of Medicine, University Paris Saclay, 94270 Le Kremlin Bicetre, France"},{"name":"INGESTEM National iPSC Infrastructure, 94800 Villejuif, France"},{"name":"CITHERA, Centre for IPSC Therapies, INSERM UMS-45, Genopole Campus, 91100 Evry, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1056\/NEJMra020777","article-title":"Chronic Myeloid Leukemia\u2014Advances in Biology and New Approaches to Treatment","volume":"349","author":"Goldman","year":"2003","journal-title":"N. 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