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Despite its unique advantages, this method often results in scaffolds with limited mechanical properties, depending on the cell type. This research aimed to enhance the mechanical properties of these constructs by culturing cells derived from various sources, including skin, bladder, urethra, vagina, and adipose tissue, on electrospun scaffolds composed of polycaprolactone and collagen (PCLCOL). The hybrid scaffolds were evaluated using various in vitro assays to assess their structural and functional properties. Results showed that different stromal cells could deposit ECM on the PCLCOL with distinct composition compared to the ECM that was self-assembled on tissue culture plates (TCP). Additionally, cells cultured on PCLCOL exhibited a different growth factor secretion profile compared to those on TCP. Mechanical testing demonstrated that the hybrid scaffolds exhibited high mechanical properties and superior manipulability. These findings suggest that PCLCOL could be a promising platform for developing biomimetic scaffolds that combine enhanced mechanical strength with integrated biological cues for tissue repair.<\/jats:p>","DOI":"10.3390\/bioengineering12101077","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T09:22:47Z","timestamp":1759483367000},"page":"1077","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Electrospun Polycaprolactone\/Collagen Scaffolds Enhance Manipulability and Influence the Composition of Self-Assembled Extracellular Matrix"],"prefix":"10.3390","volume":"12","author":[{"given":"Saeed","family":"Farzamfar","sequence":"first","affiliation":[{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Qu\u00e9bec, QC G1V 0A6, Canada"},{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center, Qu\u00e9bec, QC G1J 1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0389-667X","authenticated-orcid":false,"given":"St\u00e9phane","family":"Chabaud","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center, Qu\u00e9bec, QC G1J 1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1851-5112","authenticated-orcid":false,"given":"Julie","family":"Fradette","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Qu\u00e9bec, QC G1V 0A6, Canada"},{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center, Qu\u00e9bec, QC G1J 1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yannick","family":"Rioux","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center, Qu\u00e9bec, QC G1J 1Z4, Canada"},{"name":"D\u00e9partement de G\u00e9nie M\u00e9canique, Facult\u00e9 de Sciences et de G\u00e9nie, Universit\u00e9 Laval, Qu\u00e9bec, QC G1V 0A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6680-5563","authenticated-orcid":false,"given":"St\u00e9phane","family":"Bolduc","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Medicine, Universit\u00e9 Laval, Qu\u00e9bec, QC G1V 0A6, Canada"},{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center, Qu\u00e9bec, QC G1J 1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Assun\u00e7\u00e3o, M., Dehghan-Baniani, D., Yiu, C.H.K., Sp\u00e4ter, T., Beyer, S., and Blocki, A. 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