{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T05:24:04Z","timestamp":1762838644444,"version":"build-2065373602"},"reference-count":35,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T00:00:00Z","timestamp":1762646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2020YFA0803700"],"award-info":[{"award-number":["2020YFA0803700"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32293212","92254301"],"award-info":[{"award-number":["32293212","92254301"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Project for Young Scientists in Basic Research of the Chinese Academy of Sciences","award":["YSBR-075"],"award-info":[{"award-number":["YSBR-075"]}]},{"name":"CAMS Innovation Fund for Medical Sciences","award":["2023-I2M-2-002"],"award-info":[{"award-number":["2023-I2M-2-002"]}]},{"name":"Peking Union Medical College Hospital Outstanding Young Talent Development Program","award":["UBJ11753"],"award-info":[{"award-number":["UBJ11753"]}]},{"name":"National High Level 1072 Hospital Clinical Research Funding","award":["2025-PUMCH-C-042"],"award-info":[{"award-number":["2025-PUMCH-C-042"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>This study presents a protocol for co-culturing pancreatic islet cell clusters derived from pancreatic tissue with human umbilical vein endothelial cells (HUVECs) on Matrigel using a specialized culture medium to form vascularized pancreatic islet organoids. We established a novel culture system for vascularized pancreatic islet organoids and compared the survival and insulin secretion capabilities of pancreatic islet cells in the presence and absence of glucose stimulation. Our results indicate that matrix adhesive materials can effectively facilitate the self-assembly of the vascularized endothelial cell\u2013pancreatic islet organoids complex. Vascularized HUVEC prolongs the survival of pancreatic islet organoids in vitro. Moreover, the interaction between vascularized HUVEC and pancreatic islets significantly enhances the insulin secretion ability in response to glucose stimulation. This study reports a protocol for the long-term in vitro culture of pancreatic islet organoids, offering methods for the vascularization of pancreatic islet organoids on Matrigel. These data contribute to the understanding of how vascularization impacts the fate and function of pancreatic islet organoids, although the specific mechanism still requires further clarification.<\/jats:p>","DOI":"10.3390\/bioengineering12111222","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T08:57:55Z","timestamp":1762765075000},"page":"1222","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Protocol for the In Vitro Culturing of Vascularized Pancreatic Islet Organoids"],"prefix":"10.3390","volume":"12","author":[{"given":"Pengkun","family":"Song","sequence":"first","affiliation":[{"name":"State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junya","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Complex, Severe, and Rare Diseases, Peking Union Medical College Hospital, Beijing 100730, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China"},{"name":"Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6557-7546","authenticated-orcid":false,"given":"Lei","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Regeneration and Reconstruction, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China"},{"name":"Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1590\/2359-3997000000030","article-title":"Human pancreatic islet transplantation: An update and description of the establishment of a pancreatic islet isolation laboratory","volume":"59","author":"Rheinheimer","year":"2015","journal-title":"Arch. 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