{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T12:45:43Z","timestamp":1782823543021,"version":"3.54.5"},"reference-count":48,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Joint Fund for Regional Innovation and Development","award":["U23A20471"],"award-info":[{"award-number":["U23A20471"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>(1) Background: Postoperative anterior cruciate ligament reconstruction often involves quadriceps strength asymmetry, leading to abnormal lower limb biomechanics during running. While previous studies have examined the relationship between isokinetic strength and walking or jumping, the association between running, a key criterion for return to sport, and lower limb biomechanics remains unclear, particularly regarding isokinetic strength asymmetry at different angular velocities. (2) Methods: Isokinetic quadriceps strength, running kinematic, and kinetic data were collected from 39 ACLR individuals. Paired t-tests compared bilateral differences, and Pearson correlation analysis assessed associations between biomechanical parameters and muscle strength. (3) Results: The injured leg showed significantly weaker Qc at 60\u00b0\/s, 180\u00b0\/s, and 300\u00b0\/s (p &lt; 0.05). Compared to the uninjured leg, the injured leg demonstrated a significantly greater hip flexion angle at initial contact (p &lt; 0.05); the injured leg exhibited significantly reduced knee flexion angle at the time of peak vertical ground reaction force and peak knee flexion angle (p &lt; 0.05); the injured leg exhibited significantly reduced knee flexion moment at PVGRF, peak knee flexion moment, peak knee extension moment (p &lt; 0.05). Both the 60\u00b0\/s Qc and Qe showed moderate negative correlations with knee flexion angles, and 180\u00b0\/s Qc correlated with knee flexion moment at PVGRF (p &lt; 0.05). (4) Conclusions: ACLR patients show quadriceps strength asymmetry and abnormal sagittal knee and hip biomechanics during running. Strength symmetry moderately correlates with knee kinematics and kinetics in a velocity-dependent manner. Rehabilitation should focus on multi-speed and eccentric training with neuromuscular and hip\u2013knee coordination exercises to optimize movement and support safe return to sports.<\/jats:p>","DOI":"10.3390\/bioengineering13040400","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T12:11:44Z","timestamp":1774872704000},"page":"400","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Relationship Between Quadriceps Muscle Strength Asymmetry and Lower Limb Biomechanical Asymmetry During Running in Patients Who Underwent Anterior Cruciate Ligament Reconstruction"],"prefix":"10.3390","volume":"13","author":[{"given":"Xialin","family":"Ge","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of Sport, Tianjin 300381, China"},{"name":"Department of Sports Medicine, Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing 100191, China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries, Beijing 100191, China"},{"name":"Engineering Research Center of Sports Trauma Treatment Technology and Devices, Ministry of Education, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of Sport, Tianjin 300381, China"},{"name":"Department of Sports Medicine, Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing 100191, China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries, Beijing 100191, China"},{"name":"Engineering Research Center of Sports Trauma Treatment Technology and Devices, Ministry of Education, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Tao","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of Sport, Tianjin 300381, China"},{"name":"Department of Sports Medicine, Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing 100191, China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries, Beijing 100191, China"},{"name":"Engineering Research Center of Sports Trauma Treatment Technology and Devices, Ministry of Education, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7032-1002","authenticated-orcid":false,"given":"Longting","family":"Suo","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of Sport, Tianjin 300381, China"},{"name":"Department of Sports Medicine, Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing 100191, China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries, Beijing 100191, China"},{"name":"Engineering Research Center of Sports Trauma Treatment Technology and Devices, Ministry of Education, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9644-6434","authenticated-orcid":false,"given":"Shuang","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing 100191, China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries, Beijing 100191, China"},{"name":"Engineering Research Center of Sports Trauma Treatment Technology and Devices, Ministry of Education, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingfang","family":"Ao","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, Peking University Third Hospital, Institute of Sports Medicine of Peking University, Beijing 100191, China"},{"name":"Beijing Key Laboratory of Research and Translation for Drugs and Medical Devices in Precision Diagnosis and Treatment of Sports Injuries, Beijing 100191, China"},{"name":"Engineering Research Center of Sports Trauma Treatment Technology and Devices, Ministry of Education, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1016\/j.jsams.2008.07.005","article-title":"Incidence of anterior cruciate ligament injury and other knee ligament injuries: A national population-based study","volume":"12","author":"Gianotti","year":"2009","journal-title":"J. Sci. Med. Sport"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1177\/0363546520980079","article-title":"Quadriceps Strength Symmetry Does Not Modify Gait Mechanics After Anterior Cruciate Ligament Reconstruction, Rehabilitation, and Return-to-Sport Training","volume":"49","author":"Arhos","year":"2021","journal-title":"Am. J. Sports Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1177\/0363546510373876","article-title":"Knee function and prevalence of knee osteoarthritis after anterior cruciate ligament reconstruction: A prospective study with 10 to 15 years of follow-up","volume":"38","author":"Oiestad","year":"2010","journal-title":"Am. J. Sports Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1177\/0363546503261709","article-title":"Abnormal rotational knee motion during running after anterior cruciate ligament reconstruction","volume":"32","author":"Tashman","year":"2004","journal-title":"Am. J. Sports Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1249\/MSS.0000000000001560","article-title":"Kinetic Asymmetry during Running at Preferred and Nonpreferred Speeds","volume":"50","author":"Furlong","year":"2018","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"41","DOI":"10.32598\/JROSJ.12.1.857.1","article-title":"Interlimb Asymmetry of Vertical Ground Reaction Force as a Risk Factor for Re-injury and Knee Osteoarthritis Following Anterior Cruciate Ligament Reconstruction: A Systematic Review","volume":"12","author":"Ahmadi","year":"2025","journal-title":"J. Res. Orthop. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2792","DOI":"10.1016\/j.jbiomech.2005.10.003","article-title":"Kinetic asymmetry in female runners with and without retrospective tibial stress fractures","volume":"39","author":"Zifchock","year":"2006","journal-title":"J. Biomech."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.1177\/0363546510376053","article-title":"Biomechanical measures during landing and postural stability predict second anterior cruciate ligament injury after anterior cruciate ligament reconstruction and return to sport","volume":"38","author":"Paterno","year":"2010","journal-title":"Am. J. Sports Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1016\/j.jbiomech.2011.04.037","article-title":"Quadriceps strength and weight acceptance strategies continue to improve two years after anterior cruciate ligament reconstruction","volume":"44","author":"Roewer","year":"2011","journal-title":"J. Biomech."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"535","DOI":"10.4085\/1062-6050-478-15","article-title":"Continued Improvements in Quadriceps Strength and Biomechanical Symmetry of the Knee After Postoperative Anterior Cruciate Ligament Reconstruction Rehabilitation: Is It Time to Reconsider the 6-Month Return-to-Activity Criteria?","volume":"53","author":"Curran","year":"2018","journal-title":"J. Athl. Train."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1249\/MSS.0000000000000254","article-title":"Joint kinetics in rearfoot versus forefoot running: Implications of switching technique","volume":"46","author":"Stearne","year":"2014","journal-title":"Med. Sci. Sports Exerc."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.gaitpost.2019.07.151","article-title":"The relationship between quadriceps strength asymmetry and knee biomechanics asymmetry during walking in individuals with anterior cruciate ligament reconstruction","volume":"73","author":"Shi","year":"2019","journal-title":"Gait Posture"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"23259671231150938","DOI":"10.1177\/23259671231150938","article-title":"Relationship Between Quadriceps Strength and Knee Joint Power During Jumping After ACLR","volume":"11","author":"Graham","year":"2023","journal-title":"Orthop. J. Sports Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2961","DOI":"10.1177\/03635465221112940","article-title":"Bilateral Alterations in Isokinetic Strength and Knee Biomechanics During Side-Cutting 1 Year After Unilateral ACL Reconstruction","volume":"50","author":"Shi","year":"2022","journal-title":"Am. J. Sports Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1177\/0363546516664705","article-title":"Hamstring Strength Asymmetry at 3 Years After Anterior Cruciate Ligament Reconstruction Alters Knee Mechanics During Gait and Jogging","volume":"45","author":"Abourezk","year":"2017","journal-title":"Am. J. Sports Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"7269","DOI":"10.1038\/s41598-017-06601-5","article-title":"Isokinetic angle-specific moments and ratios characterizing hamstring and quadriceps strength in anterior cruciate ligament deficient knees","volume":"7","author":"Huang","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1055\/s-2007-972836","article-title":"Isokinetic muscle performance after anterior cruciate ligament surgery. Long-term results and outcome predicting factors after primary surgery and late-phase reconstruction","volume":"17","author":"Natri","year":"1996","journal-title":"Int. J. Sports Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"60","DOI":"10.2519\/jospt.1994.20.2.60","article-title":"The relationship between subjective knee scores, isokinetic testing, and functional testing in the ACL-reconstructed knee","volume":"20","author":"Wilk","year":"1994","journal-title":"J. Orthop. Sports Phys. Ther."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"101543","DOI":"10.1016\/j.rehab.2021.101543","article-title":"Isokinetic quadriceps symmetry helps in the decision to return to running after anterior cruciate ligament reconstruction","volume":"65","author":"Dauty","year":"2022","journal-title":"Ann. Phys. Rehabil. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"960","DOI":"10.2519\/jospt.2018.8170","article-title":"Bilateral Alterations in Running Mechanics and Quadriceps Function Following Unilateral Anterior Cruciate Ligament Reconstruction","volume":"48","author":"Pamukoff","year":"2018","journal-title":"J. Orthop. Sports Phys. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"750","DOI":"10.2519\/jospt.2012.4194","article-title":"The impact of quadriceps femoris strength asymmetry on functional performance at return to sport following anterior cruciate ligament reconstruction","volume":"42","author":"Schmitt","year":"2012","journal-title":"J. Orthop. Sports Phys. Ther."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.csm.2011.10.001","article-title":"The anatomy and biomechanics of running","volume":"31","author":"Nicola","year":"2012","journal-title":"Clin. Sports Med."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Hill, M., Kiesewetter, P., Milani, T.L., and Mitschke, C. (2024). An Investigation of Running Kinematics with Recovered Anterior Cruciate Ligament Reconstruction on a Treadmill and In-Field Using Inertial Measurement Units: A Preliminary Study. Bioengineering, 11.","DOI":"10.3390\/bioengineering11040404"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/S0268-0033(01)00097-3","article-title":"The effect of insufficient quadriceps strength on gait after anterior cruciate ligament reconstruction","volume":"17","author":"Lewek","year":"2002","journal-title":"Clin. Biomech."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"23259671211063800","DOI":"10.1177\/23259671211063800","article-title":"Relationship Between Isokinetic Knee Strength and Single-Leg Drop Jump Performance 9 Months After ACL Reconstruction","volume":"10","author":"Crotty","year":"2022","journal-title":"Orthop. J. Sports Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1123\/jsr.2020-0479","article-title":"Mechanisms of Arthrogenic Muscle Inhibition","volume":"31","author":"Lepley","year":"2022","journal-title":"J. Sport. Rehabil."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1136\/bjsports-2016-096031","article-title":"Simple decision rules can reduce reinjury risk by 84% after ACL reconstruction: The Delaware-Oslo ACL cohort study","volume":"50","author":"Grindem","year":"2016","journal-title":"Br. J. Sports Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2438","DOI":"10.1177\/0363546520940310","article-title":"A Cross-sectional Examination of Quadriceps Strength, Biomechanical Function, and Functional Performance from 9 to 24 Months After Anterior Cruciate Ligament Reconstruction","volume":"48","author":"Curran","year":"2020","journal-title":"Am. J. Sports Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.csm.2008.02.001","article-title":"Maximizing quadriceps strength after ACL reconstruction","volume":"27","author":"Thomas","year":"2008","journal-title":"Clin. Sports Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.knee.2024.10.010","article-title":"Hip and knee joints mechanics and asymmetries in individuals with a history of anterior cruciate ligament reconstruction during overground running","volume":"52","author":"Shahbazi","year":"2025","journal-title":"Knee"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.1016\/j.jbiomech.2013.06.033","article-title":"Gluteus maximus and soleus compensate for simulated quadriceps atrophy and activation failure during walking","volume":"46","author":"Thompson","year":"2013","journal-title":"J. Biomech."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"515","DOI":"10.5435\/JAAOS-D-16-00532","article-title":"Natural History of Structural Hip Abnormalities and the Potential for Hip Preservation","volume":"26","author":"Wylie","year":"2018","journal-title":"J. Am. Acad. Orthop. Surg."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1007\/s11999-007-0060-z","article-title":"The etiology of osteoarthritis of the hip: An integrated mechanical concept","volume":"466","author":"Ganz","year":"2008","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1007\/s00167-017-4810-5","article-title":"Poor knee function after ACL reconstruction is associated with attenuated landing force and knee flexion moment during running","volume":"26","author":"Perraton","year":"2018","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2607","DOI":"10.1177\/03635465211026665","article-title":"Running Biomechanics Before Injury and 1 Year After Anterior Cruciate Ligament Reconstruction in Division I Collegiate Athletes","volume":"49","author":"Knurr","year":"2021","journal-title":"Am. J. Sports Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"303","DOI":"10.4085\/1062-6050-49.5.06","article-title":"Persistent neuromuscular and corticomotor quadriceps asymmetry after anterior cruciate ligament reconstruction","volume":"50","author":"Kuenze","year":"2015","journal-title":"J. Athl. Train."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1007\/s00221-019-05499-x","article-title":"Quadriceps muscle function following anterior cruciate ligament reconstruction: Systemic differences in neural and morphological characteristics","volume":"237","author":"Lepley","year":"2019","journal-title":"Exp. Brain Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.gaitpost.2020.10.028","article-title":"The effect of cognitive loading on the lower extremity movement coordination variability in patients with anterior cruciate ligament reconstruction","volume":"84","author":"Shi","year":"2021","journal-title":"Gait Posture"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"806","DOI":"10.4085\/1062-6050-49.3.35","article-title":"Osteoarthritis prevalence following anterior cruciate ligament reconstruction: A systematic review and numbers-needed-to-treat analysis","volume":"49","author":"Luc","year":"2014","journal-title":"J. Athl. Train."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1007\/s40279-019-01120-x","article-title":"Running Biomechanics in Individuals with Anterior Cruciate Ligament Reconstruction: A Systematic Review","volume":"49","author":"Willy","year":"2019","journal-title":"Sports Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"871","DOI":"10.2106\/00004623-199072060-00012","article-title":"Gait adaptations by patients who have a deficient anterior cruciate ligament","volume":"72","author":"Berchuck","year":"1990","journal-title":"J. Bone Joint Surg. Am."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.1152\/japplphysiol.00146.2013","article-title":"Eccentric exercise: Mechanisms and effects when used as training regime or training adjunct","volume":"116","author":"Vogt","year":"2014","journal-title":"J. Appl. Physiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1177\/0363546515578252","article-title":"Quadriceps Strength Asymmetry After Anterior Cruciate Ligament Reconstruction Alters Knee Joint Biomechanics and Functional Performance at Time of Return to Activity","volume":"43","author":"Lepley","year":"2015","journal-title":"Am. J. Sports Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1097\/JSM.0000000000000723","article-title":"Neuromuscular Training Improves Biomechanical Deficits at the Knee in Anterior Cruciate Ligament-Reconstructed Athletes","volume":"31","author":"Nagelli","year":"2021","journal-title":"Clin. J. Sport. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.1177\/0363546515602016","article-title":"Young Athletes with Quadriceps Femoris Strength Asymmetry at Return to Sport After Anterior Cruciate Ligament Reconstruction Demonstrate Asymmetric Single-Leg Drop-Landing Mechanics","volume":"43","author":"Ithurburn","year":"2015","journal-title":"Am. J. Sports Med."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1136\/bjsports-2015-094797","article-title":"Knee kinematics and joint moments during gait following anterior cruciate ligament reconstruction: A systematic review and meta-analysis","volume":"50","author":"Hart","year":"2016","journal-title":"Br. J. Sports Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2772","DOI":"10.1177\/0363546518787505","article-title":"Biomechanical Deficits at the Hip in Athletes with ACL Reconstruction Are Ameliorated with Neuromuscular Training","volume":"46","author":"Nagelli","year":"2018","journal-title":"Am. J. Sports Med."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2325967120959347","DOI":"10.1177\/2325967120959347","article-title":"Neuromuscular Training Improves Self-Reported Function and Single-Leg Landing Hip Biomechanics in Athletes After Anterior Cruciate Ligament Reconstruction","volume":"8","author":"Nagelli","year":"2020","journal-title":"Orthop. J. Sports Med."}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/13\/4\/400\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T12:27:13Z","timestamp":1774873633000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/13\/4\/400"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,30]]},"references-count":48,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["bioengineering13040400"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering13040400","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,30]]}}}