{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:58:07Z","timestamp":1792789087109,"version":"4.5.0"},"reference-count":52,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:00:00Z","timestamp":1776038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NSERC\/FRQNT NOVA program","award":["ALLRP 577205-2022"],"award-info":[{"award-number":["ALLRP 577205-2022"]}]},{"DOI":"10.13039\/501100002914","name":"Concordia University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002914","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/01qxhf360","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Biosurfactants offer a green, sustainable approach to many environmental bioremediations, especially for oil contamination. In this study, the aim is to evaluate the effectiveness of biosurfactants in accelerating hydrocarbon removal from mature fine tailings under anaerobic conditions. The bacteria were isolated from mature fine tailings and tested for biosurfactant production using different biosurfactant screening methods (i.e., blood agar, cetyltrimethylammonium bromide (CTAB) blue agar, oil displacement, and drop collapse). The most efficient strain showed high similarity to Stutzerimonas stutzeri by 16S rRNA gene sequencing. Results showed that this strain produces rhamnolipids with a critical micelle concentration (CMC) of 600 mg\/L and a minimum surface tension of 38.70 \u00b1 0.08 mN\/m. Moreover, when supplemented with whey, the strain showed a high emulsification index of 24 toward toluene (66%) and hexane (60%). The bioremediation of mature fine tailings (MFTs) was conducted under anaerobic conditions by adding a consortium of the four strains that were positive in biosurfactant screening tests. The results showed 53% removal of n-alkane C9-C30 and a reduction in surface tension from 69 \u00b1 0.5 mN\/m to a minimum of 54.33 \u00b1 0.5 mN\/m. The results suggest the potential successful application of bioaugmentation for in situ biological treatment in the oil sands industry.<\/jats:p>","DOI":"10.3390\/bioengineering13040452","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T10:19:14Z","timestamp":1776075554000},"page":"452","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of the Biosurfactant Produced by Indigenous Bacteria from Mature Fine Tailings"],"prefix":"10.3390","volume":"13","author":[{"given":"Shima","family":"Shojaei","sequence":"first","affiliation":[{"name":"Department of Building, Civil and Environmental Engineering, Concordia University, 1455 de Maisonneuve Blvd. W., Montreal, QC H3G 1M8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5337-4776","authenticated-orcid":false,"given":"Catherine N.","family":"Mulligan","sequence":"additional","affiliation":[{"name":"Department of Building, Civil and Environmental Engineering, Concordia University, 1455 de Maisonneuve Blvd. W., Montreal, QC H3G 1M8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5459","DOI":"10.1021\/es060993m","article-title":"Biodegradation of Short-Chain n-Alkanes in Oil Sands Tailings under Methanogenic Conditions","volume":"40","author":"Siddique","year":"2006","journal-title":"Environ. Sci. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100526","DOI":"10.1016\/j.eti.2019.100526","article-title":"Remediation of Soil and Water Contaminated with Petroleum Hydrocarbon: A Review","volume":"17","author":"Ossai","year":"2020","journal-title":"Environ. Technol. Innov."},{"key":"ref_3","first-page":"1","article-title":"Bioremediation of Gasoline Contaminated Agricultural Soil by Bioaugmentation","volume":"7","author":"Nwankwegu","year":"2017","journal-title":"Environ. Sci. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1007\/s13205-022-03277-1","article-title":"A Review on Biosurfactant Producing Bacteria for Remediation of Petroleum Contaminated Soils","volume":"12","author":"Sah","year":"2022","journal-title":"3 Biotech"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"9010","DOI":"10.1007\/s12010-024-05000-7","article-title":"Production of Lipopeptide Biosurfactant Using Wastewater from Parboiled Paddy Rice and Evaluation of Antifungal Property of the Biosurfactant Against Two Dermatophyte Fungi","volume":"196","author":"Malakar","year":"2024","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"123585","DOI":"10.1016\/j.molliq.2023.123585","article-title":"Exploring the Role of Biosurfactants in the Remediation of Organic Contaminants with a Focus on the Mechanism- a Review","volume":"393","author":"Bose","year":"2024","journal-title":"J. Mol. Liq."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mulligan, C.N., Sharma, S.K., Mudhoo, A., and Makhijani, K. (2014). Green Chemistry and Biosurfactant Research. Biosurfactant Research Trends and Application, CRC press Taylor& Francis Group.","DOI":"10.1201\/b16383-2"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.envpol.2004.06.009","article-title":"Environmental applications for biosurfactants","volume":"133","author":"Mulligan","year":"2005","journal-title":"Environ. Pollut."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Zhou, J., Zheng, Y., Shen, Y., Zhang, X., and Chen, S. (2019). High Di-Rhamnolipid Production by Pseudomonas aeruginosa KT1115 through Fermentation Optimization. Front. Bioeng. Biotechnol., 7.","DOI":"10.3389\/fbioe.2019.00245"},{"key":"ref_10","first-page":"1","article-title":"Selection of Pseudomonas aeruginosa for biosurfactant production and studies of its antimicrobial activity","volume":"23","author":"Doheim","year":"2014","journal-title":"Egypt. J. Pet."},{"key":"ref_11","first-page":"27470","article-title":"Characterization and properties of the biosurfactant produced by PAH-degrading bacteria isolated from contaminated oily sludge environment","volume":"26","author":"Tripathi","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_12","first-page":"49848","article-title":"Isolation, screening and molecular characterization of biosurfactant producing bacteria from soil samples of auto repair shops","volume":"28","author":"Uyar","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_13","first-page":"11","article-title":"Isolation, identification and molecular characterization of crude oil degrading novel Pseudomonas aeruginosa strain MR21 from oil polluted Mumbai Harbor","volume":"4","author":"Mohanram","year":"2022","journal-title":"Int. J. Environ. Health Sci."},{"key":"ref_14","first-page":"2973","article-title":"Anaerobic biodegradation of hydrocarbons in oil sands tailings","volume":"72","author":"Siddique","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"fix034","DOI":"10.1093\/femsec\/fix034","article-title":"The microbiology of oil sands tailings: Past, present and future","volume":"93","author":"Foght","year":"2017","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1139\/W10-029","article-title":"Mature fine tailings from oil sands processing harbour diverse methanogenic communities","volume":"56","author":"Penner","year":"2010","journal-title":"Can. J. Microbiol."},{"key":"ref_17","unstructured":"Rezaeitamijani, M., and Mulligan, C.N. (2022, January 2\u20135). Evaluation of hydrocarbon degradation by indigenous bacteria degrading residual hydrocarbons of mature fine tailings under anaerobic conditions. Proceedings of the GeoCalgary 2022: Reflection on Resources, Calgary, AB, Canada."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1002\/jsde.12021","article-title":"Characterization of Biosurfactant Produced by a Novel Strain of Pseudomonas aeruginosa, Isolate ADMT1","volume":"21","author":"Tiwary","year":"2018","journal-title":"J. Surfactants Deterg."},{"key":"ref_19","first-page":"4035","article-title":"Two alternative methods for anaerobic cultivation of Bacteroides fragilis ATCC 25285 compared to the efficiency of cultivation in a GasPak system","volume":"27","author":"Ermenlieva","year":"2021","journal-title":"J. Int. Med. Assoc. Bulg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/s12934-018-0934-7","article-title":"Bacterial polyextremotolerant bioemulsifiers from arid soils improve water retention capacity and humidity uptake in sandy soil","volume":"17","author":"Raddadi","year":"2018","journal-title":"Microb. Cell Fact."},{"key":"ref_21","unstructured":"Walter, V., Syldatk, C., and Hausmann, R. (2013). Screening concepts for the isolation of biosurfactant-producing microorganisms. Madame Curie Bioscience Database, Landes Bioscience."},{"key":"ref_22","first-page":"311","article-title":"Selection of microbes producing biosurfactants in media without hydrocarbons","volume":"62","author":"Mulligan","year":"1984","journal-title":"J. Ferment. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"211003","DOI":"10.1098\/rsos.211003","article-title":"Production Optimization, Stability and Oil Emulsifying Potential of Biosurfactants from Selected Bacteria Isolated from Oil-Contaminated Sites","volume":"8","author":"Ali","year":"2021","journal-title":"R. Soc. Open Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/BF02438660","article-title":"New Method for Detecting Rhamnolipids Excreted by Pseudomonas Species during Growth on Mineral Agar","volume":"5","author":"Siegmund","year":"1991","journal-title":"Biotechnol. Tech."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.colsurfb.2012.01.043","article-title":"Production and Characterization of Biosurfactant Produced by a Novel Pseudomonas sp. 2B","volume":"95","author":"Aparna","year":"2012","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Silva, T.P., Paixao, S.M., Tavares, J., Gil, C.V., Torres, C.A.V., and Alves, L. (2022). A New Biosurfactant\/Bioemulsifier from Gordonia alkanivorans Strain 1B: Production and Characterization. Processes, 10.","DOI":"10.3390\/pr10050845"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.ejbt.2020.05.001","article-title":"Biosurfactant Produced by Candida Utilis UFPEDA1009 with Potential Application in Cookie Formulation","volume":"46","author":"Ribeiro","year":"2020","journal-title":"Electron. J. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.jbiosc.2011.10.002","article-title":"Biosurfactant-producing bacterium, Pseudomonas aeruginosa MA01 isolated from spoiled apples: Physicochemical and structural characteristics of isolated biosurfactant","volume":"113","author":"Abbasi","year":"2012","journal-title":"J. Biosci. Bioeng."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3637","DOI":"10.1007\/s11756-023-01513-4","article-title":"Degradation of Crude Oil and the Pure Hydrocarbon Fractions by Indigenous Soil Microorganisms","volume":"78","author":"Mohammed","year":"2023","journal-title":"Biologia"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"106707","DOI":"10.1016\/j.mimet.2023.106707","article-title":"Microliter Spotting and Micro-Colony Observation: A Rapid and Simple Approach for Counting Bacterial Colony Forming Units","volume":"207","author":"Bhuyan","year":"2023","journal-title":"J. Microbiol. Methods"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1021\/es9809758","article-title":"Extraction of Petroleum Hydrocarbons from Soil by Mechanical Shaking","volume":"33","author":"Schwab","year":"1999","journal-title":"Environ. Sci. Technol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1080\/15275922.2011.574312","article-title":"Chemical Fingerprints of Alberta Oil Sands and Related Petroleum Products","volume":"12","author":"Yang","year":"2011","journal-title":"Environ. Forensics"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Rani, M., Weadge, J.T., and Jabaji, S. (2020). Isolation and Characterization of Biosurfactant-Producing Bacteria From Oil Well Batteries With Antimicrobial Activities Against Food-Borne and Plant Pathogens. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.00064"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"212974","DOI":"10.1016\/j.geoen.2024.212974","article-title":"Screening of Sustainable Biosurfactant Produced by Indigenous Bacteria Isolated from Egyptian Oil Fields for Microbial Enhanced Oil Recovery","volume":"239","author":"Raouf","year":"2024","journal-title":"Geoenergy Sci. Eng."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.sjbs.2014.11.018","article-title":"Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia","volume":"22","author":"Elazzazy","year":"2015","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/j.ijbiomac.2018.01.209","article-title":"Investigation of the structural, physicochemical properties, and aggregation behavior of lipopeptide biosurfactant produced by Acinetobacter junii B6","volume":"112","author":"Ohadi","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.1039\/D2RA05967C","article-title":"Graphene quantum dots functionalized with rhamnolipid produced from bioconversion of palm kernel oil by Pseudomonas stutzeri BK-AB12MT as a photocatalyst","volume":"13","author":"Habibah","year":"2023","journal-title":"RSC Adv."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.biortech.2012.10.127","article-title":"Coal-induced production of a rhamnolipid biosurfactant by Pseudomonas stutzeri isolated from the formation water of the Jharia coalbed","volume":"128","author":"Singh","year":"2013","journal-title":"Bioresour. Technol."},{"key":"ref_39","first-page":"43","article-title":"Molecular identification and characterization of Pseudomonas stutzeri strain showing potent degradation activity towards pyrene","volume":"33","author":"Ali","year":"2023","journal-title":"J. King Abdulaziz Univ. Sci."},{"key":"ref_40","first-page":"533","article-title":"Cell Biology and Molecular Basis of Denitrification","volume":"61","author":"Zumft","year":"1997","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1016\/S0043-1354(99)00287-0","article-title":"Control of pH during denitrification in subsurface sediment microcosms using encapsulated phosphate buffer","volume":"34","author":"Rust","year":"2000","journal-title":"Water Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"25364","DOI":"10.1038\/s41598-024-77136-9","article-title":"Microbial community acclimation during anaerobic digestion of high-oil food waste","volume":"14","author":"Hu","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1007\/s11274-021-02999-3","article-title":"Effect of consortium bioaugmentation and biostimulation on remediation efficiency and bacterial diversity of diesel-contaminated aged soil","volume":"37","author":"Chaudhary","year":"2021","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3168","DOI":"10.1080\/09593330.2025.2458796","article-title":"Assessment of bioremediation potential of Stutzerimonas stutzeri NL3 and Enterobacter cloacae NL4 isolates from an Algerian oil field","volume":"46","author":"Lenchi","year":"2025","journal-title":"Environ. Technol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1007\/s13205-017-0902-7","article-title":"Biosurfactant and enzyme mediated crude oil degradation by Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3","volume":"7","author":"Parthipan","year":"2017","journal-title":"3 Biotech"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Romantschuk, M., Lahti-Leikas, K., Kontro, M., Galitskaya, P., Talvenm\u00e4ki, H., Simpanen, S., Allen, J.A., and Sinkkonen, A. (2023). Bioremediation of contaminated soil and groundwater by in situ biostimulation. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1258148"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"5892","DOI":"10.1021\/es200649t","article-title":"Anaerobic biodegradation of longer-chain n-alkanes coupled to methane production in oil sands tailings","volume":"45","author":"Siddique","year":"2011","journal-title":"Environ. Sci. Technol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"100507","DOI":"10.1016\/j.jgeb.2025.100507","article-title":"Bioremediation of crude oil and heavy metal pollutants in marine environment by biosurfactant, rhamnolipid isolated from Stutzerimonas stutzeri MW15","volume":"23","author":"Sony","year":"2025","journal-title":"J. Genet. Eng. Biotechnol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.colsurfb.2008.11.018","article-title":"An efficient biosurfactant-producing bacterium Pseudomonas aeruginosa MR01, isolated from oil excavation areas in south of Iran","volume":"69","author":"Shourian","year":"2009","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Zhang, T., and Zhang, H. (2022). Microbial Consortia Are Needed to Degrade Soil Pollutants. Microorganisms, 10.","DOI":"10.3390\/microorganisms10020261"},{"key":"ref_51","unstructured":"Cao, Z., Liu, X., Wang, Q., and Wang, Y. (2022). Construction of microbial consortia for the degradation of environmental pollutants. Front. Microbiol., 13."},{"key":"ref_52","first-page":"2154","article-title":"Reconstruction and evaluation of oil-degrading bacterial consortia for crude oil biodegradation","volume":"11","author":"Ma","year":"2021","journal-title":"Sci. Rep."}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/13\/4\/452\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T04:15:46Z","timestamp":1776226546000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/13\/4\/452"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,13]]},"references-count":52,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["bioengineering13040452"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering13040452","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,13]]}}}