{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:15:41Z","timestamp":1785402941628,"version":"3.56.0"},"reference-count":30,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science and Technology Innovation 2030, Major Project\u2014Research on Cancer, Cardiovascular, Respiratory and Metabolic Diseases","award":["2025ZD0552402"],"award-info":[{"award-number":["2025ZD0552402"]}]},{"name":"National High level Hospital Clinical Research Funding","award":["2025-PUMCH-C-042"],"award-info":[{"award-number":["2025-PUMCH-C-042"]}]},{"name":"National High level Hospital Clinical Research Funding","award":["PUMCH-H-010"],"award-info":[{"award-number":["PUMCH-H-010"]}]},{"name":"Peking Union Medical College Hospital Outstanding Young Talent Development Program","award":["UBJ11753"],"award-info":[{"award-number":["UBJ11753"]}]},{"award":["UBJ11753"],"award-info":[{"award-number":["UBJ11753"]}],"id":[{"id":"https:\/\/ror.org\/04jztag35","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Beijing High-Level Innovation and Entrepreneurship Talent Support Program Young Backbone Talent Projects","award":["G202532187"],"award-info":[{"award-number":["G202532187"]}]},{"name":"Peking Union Medical College Hospital Talent Cultivation Program","award":["UHB50192"],"award-info":[{"award-number":["UHB50192"]}]},{"award":["UHB50192"],"award-info":[{"award-number":["UHB50192"]}],"id":[{"id":"https:\/\/ror.org\/04jztag35","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Extracellular Vesicles (EVs) have been increasingly recognized as mediators of intercellular communication in pancreatic tissues, carrying molecular cargo reflective of their cellular origin; however, their direct isolation from solid tissue remains technically challenging due to the high enzymatic activity, lipid content, and structural fragility of the pancreas. Here, we establish a pancreas-adapted and reproducible workflow for the isolation of EVs directly from human and mouse pancreatic tissues across tumor and non-tumorous contexts. This approach integrates controlled tissue processing, gentle enzymatic-mechanical dissociation, and sequential centrifugation to enable efficient vesicle recovery while preserving structural integrity and minimizing contamination. The isolated EVs exhibited characteristic size distributions, intact morphology, and enrichment of canonical EV markers, accompanied by depletion of intracellular components. Quantitative analyses demonstrated high reproducibility across independent isolations, with a coefficient of variation of less than 6%. Proteomic profiling revealed selective enrichment of vesicle-associated proteins and preservation of molecular differences between tumor and normal pancreatic tissues, indicating that tissue-derived EVs retain disease-associated protein signatures. Together, these results establish a standardized framework for pancreas-derived EV isolation that supports reproducible downstream analyses and facilitates molecular characterization of pancreatic tissues.<\/jats:p>","DOI":"10.3390\/bioengineering13080879","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T08:09:42Z","timestamp":1785398982000},"page":"879","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and Characterization of Extracellular Vesicles from Human and Mouse Pancreatic Tissue"],"prefix":"10.3390","volume":"13","author":[{"given":"Junya","family":"Peng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Complex and Severe and Rare Diseases, Peking Union Medical College Hospital, Beijing 102602, China"},{"name":"Institute of Clinical Medicine, Peking Union Medical College Hospital, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lulu","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, Peking Union Medical College Hospital, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyang","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China"},{"name":"4 + 4 Medical Doctor Program, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Han","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, Peking Union Medical College Hospital, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenmin","family":"Tian","sequence":"additional","affiliation":[{"name":"Center for Precision Medicine Multi-Omics Research, Institute of Advanced Clinical Medicine, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, Center for Precision Medicine Multi-Omics Research, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yupei","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Complex and Severe and Rare Diseases, Peking Union Medical College Hospital, Beijing 102602, China"},{"name":"Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianlin","family":"Han","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Peking Union Medical College Hospital, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/j.tcb.2023.01.002","article-title":"Extracellular Vesicles and Nanoparticles: Emerging Complexities","volume":"33","author":"Jeppesen","year":"2023","journal-title":"Trends Cell Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"36405","DOI":"10.31083\/FBL36405","article-title":"Extracellular Vesicles: Recent Advances and Perspectives","volume":"30","author":"Ragni","year":"2025","journal-title":"Front. Biosci. Landmark Ed."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4098","DOI":"10.7150\/ijbs.97076","article-title":"Exosomes: Emerging Insights into the Progression of Pancreatic Cancer","volume":"20","author":"Zhou","year":"2024","journal-title":"Int. J. Biol. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Qiao, Y., Yin, H., Zhang, Y., Zhang, J., and Dong, Q. (2025). Domestication and Feedback: Bidirectional Hijacking in Pancreatic Ductal Adenocarcinoma Microenvironment. Front. Immunol., 16.","DOI":"10.3389\/fimmu.2025.1585858"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1080\/14737159.2025.2545968","article-title":"A New Perspective for the Diagnosis of Pancreatic Cancer: Based on the Understanding of Extracellular Vesicle Proteins","volume":"25","author":"Dong","year":"2025","journal-title":"Expert. Rev. Mol. Diagn."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"23712","DOI":"10.1038\/s41598-025-08826-1","article-title":"Radiation Suppresses Liver Metastases via Exosome Secretion in Pancreatic Cancer Cells","volume":"15","author":"Nakaoka","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1245\/s10434-024-16115-w","article-title":"miR-6855-5p Enhances Radioresistance and Promotes Migration of Pancreatic Cancer by Inducing Epithelial-Mesenchymal Transition via Suppressing FOXA1: Potential of Plasma Exosomal miR-6855-5p as an Indicator of Radiosensitivity in Patients with Pancreatic Cancer","volume":"32","author":"Ueda","year":"2025","journal-title":"Ann. Surg. Oncol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2950","DOI":"10.1016\/j.ymthe.2024.06.025","article-title":"Prospects and Challenges of Tissue-Derived Extracellular Vesicles","volume":"32","author":"Lee","year":"2024","journal-title":"Mol. Ther."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e70059","DOI":"10.1002\/jex2.70059","article-title":"Detection and Isolation of Tissue-Specific Extracellular Vesicles From the Blood","volume":"4","author":"Newman","year":"2025","journal-title":"J. Extracell. Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1038\/nrgastro.2013.36","article-title":"The Role of Protein Synthesis and Digestive Enzymes in Acinar Cell Injury","volume":"10","author":"Logsdon","year":"2013","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"E968","DOI":"10.1073\/pnas.1521230113","article-title":"Proteomic Comparison Defines Novel Markers to Characterize Heterogeneous Populations of Extracellular Vesicle Subtypes","volume":"113","author":"Kowal","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"102464","DOI":"10.1016\/j.xpro.2023.102464","article-title":"Protocol to Isolate Human Normal and Neoplastic Pancreatic Cells for Single-Cell Omic Analyses","volume":"4","author":"Chen","year":"2023","journal-title":"STAR Protoc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e12404","DOI":"10.1002\/jev2.12404","article-title":"Minimal Information for Studies of Extracellular Vesicles (MISEV2023): From Basic to Advanced Approaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e70185","DOI":"10.1002\/jev2.70185","article-title":"Recommendations for Studying In Situ Extracellular Vesicles From Solid Tissue","volume":"14","author":"Crescitelli","year":"2025","journal-title":"J. Extracell. Vesicles"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5335","DOI":"10.1038\/s41598-019-41800-2","article-title":"Comparative Analysis of Exosome Isolation Methods Using Culture Supernatant for Optimum Yield, Purity and Downstream Applications","volume":"9","author":"Patel","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.3892\/ijmm.2016.2759","article-title":"Charge-Based Precipitation of Extracellular Vesicles","volume":"38","author":"Deregibus","year":"2016","journal-title":"Int. J. Mol. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.1136\/gutjnl-2021-324994","article-title":"Extracellular Vesicles from Pancreatic Cancer Stem Cells Lead an Intratumor Communication Network (EVNet) to Fuel Tumour Progression","volume":"71","author":"Ruivo","year":"2022","journal-title":"Gut"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1038\/s42003-021-02364-y","article-title":"HLA-B and Cysteinylated Ligands Distinguish the Antigen Presentation Landscape of Extracellular Vesicles","volume":"4","author":"Heck","year":"2021","journal-title":"Commun. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"11239","DOI":"10.1038\/s41598-019-47519-4","article-title":"Keratin 17 Identifies the Most Lethal Molecular Subtype of Pancreatic Cancer","volume":"9","author":"Leiton","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1186\/s13046-022-02516-w","article-title":"LAMC2 Marks a Tumor-Initiating Cell Population with an Aggressive Signature in Pancreatic Cancer","volume":"41","author":"Cave","year":"2022","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.1016\/j.ccell.2026.03.021","article-title":"A Conserved Re-Epithelialization Program Underlies Malignancy in Pancreatic Ductal Adenocarcinoma","volume":"44","author":"Zhuo","year":"2026","journal-title":"Cancer Cell"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1053\/j.gastro.2024.03.007","article-title":"Genetic Deletion of Galectin-3 Inhibits Pancreatic Cancer Progression and Enhances the Efficacy of Immunotherapy","volume":"167","author":"Yang","year":"2024","journal-title":"Gastroenterology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"10543","DOI":"10.1038\/s41467-024-54850-6","article-title":"Epstein-Barr Virus Infection Upregulates Extracellular OLFM4 to Activate YAP Signaling during Gastric Cancer Progression","volume":"15","author":"Wen","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ohkuma, R., Yada, E., Ishikawa, S., Komura, D., Ishizaki, H., Tamada, K., Kubota, Y., Hamada, K., Ishida, H., and Hirasawa, Y. (2020). High Expression of Olfactomedin-4 Is Correlated with Chemoresistance and Poor Prognosis in Pancreatic Cancer. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0226707"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2447","DOI":"10.21037\/jgo-24-374","article-title":"Systematic Review and Meta-Analysis of the Prognostic Role of Fibroblast-Activation Protein in Gastrointestinal Cancers","volume":"15","author":"Sunami","year":"2024","journal-title":"J. Gastrointest. Oncol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/s44164-025-00083-w","article-title":"Pancreatic Cancer Extracellular Vesicles Stimulate Schwann Cell Activation and Perineural Invasion in Vitro via IL-8\/CCL2","volume":"4","author":"Gregory","year":"2025","journal-title":"Vitr. Models"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.pan.2023.04.002","article-title":"Exosomes in Ascites from Patients with Human Pancreatic Cancer Enhance Remote Metastasis Partially through Endothelial-Mesenchymal Transition","volume":"23","author":"Kimoto","year":"2023","journal-title":"Pancreatology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e202400329","DOI":"10.1002\/biot.202400329","article-title":"Comparison of Lung Tissue-Derived Extracellular Vesicles Using Multiple Dissociation Methods for Profiling Protein Biomarkers","volume":"19","author":"Han","year":"2024","journal-title":"Biotechnol. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1038\/s41596-020-00466-1","article-title":"Isolation and Characterization of Extracellular Vesicle Subpopulations from Tissues","volume":"16","author":"Crescitelli","year":"2021","journal-title":"Nat. Protoc."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1348885","DOI":"10.1080\/20013078.2017.1348885","article-title":"A Rigorous Method to Enrich for Exosomes from Brain Tissue","volume":"6","author":"Vella","year":"2017","journal-title":"J. Extracell. 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