{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T05:18:35Z","timestamp":1787980715609,"version":"build-2784847793"},"reference-count":65,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>The treatment of articular cartilage defects remains a significant clinical challenge due to the tissue\u2019s limited intrinsic repair capacity. This paper presents a review of clinical experiences with the use of Wharton\u2019s jelly-derived mesenchymal stem cells (WJ-MSCs) as a novel therapeutic option for cartilage regeneration. WJ-MSCs offer key advantages, including high proliferative potential, strong immunomodulatory properties, and low immunogenicity, making them suitable for allogeneic applications. This review describes a single-step, dry-arthroscopic technique that was employed for the implantation of WJ-MSCs embedded in a scaffold directly into cartilage defects. Clinical follow-up, supported by MRI evaluation, demonstrated favorable outcomes with evidence of defect filling, improved cartilage surface quality, and sustained functional improvement in patients. These results suggest that WJ-MSC-based therapies, delivered through minimally invasive surgical techniques, represent a safe and effective strategy for cartilage repair, with the potential to become an important alternative to current standard treatments. Recent advances in artificial intelligence (AI) and multimodal bioprinting are opening new perspectives for standardizing regenerative therapies. Machine learning models can predict bioink performance, optimize scaffold design, and integrate real-time imaging feedback such as optical coherence tomography and photoacoustic imaging. These approaches allow closed-loop quality control and the creation of digital twins to ensure biomechanical fidelity of constructs. Incorporating AI-assisted bioprinting with Wharton\u2019s jelly MSCs could accelerate the translation of laboratory findings into reproducible, patient-specific cartilage implants. This manuscript is structured as a translational review of WJ-MSC-based cartilage repair, with AI-assisted bioprinting presented as a prospective future manufacturing direction rather than current clinical practice.<\/jats:p>","DOI":"10.3390\/bioengineering13090995","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T16:47:13Z","timestamp":1787849233000},"page":"995","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Next-Generation Cartilage Repair: Clinical Use of Wharton\u2019s Jelly MSCs and the Emerging Role of AI-Assisted Bioprinting"],"prefix":"10.3390","volume":"13","author":[{"given":"Bogus\u0142aw","family":"Sadlik","sequence":"first","affiliation":[{"name":"Orthopaedy Facility, College of Physiotherapy in Wroclaw, 54-430 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Matuszewska","sequence":"additional","affiliation":[{"name":"Go on Clinic, 54-430 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wojciech","family":"Klon","sequence":"additional","affiliation":[{"name":"St. Luke\u2019s Hospital, 43-309 Bielsko-Bia\u0142a, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8935-4811","authenticated-orcid":false,"given":"Ewa","family":"Stodolak-Zych","sequence":"additional","affiliation":[{"name":"Department of Biomaterials and Composites, AGH University of Science and Technology, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5212-5484","authenticated-orcid":false,"given":"Kamila","family":"Rawoj\u0107","sequence":"additional","affiliation":[{"name":"Department of Biocybernetics and Biomedical Engineering, AGH University of Science and Technology, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"819","DOI":"10.3928\/0147-7447-19940901-25","article-title":"Biological resurfacing: An alternative to total joint arthroplasty","volume":"17","author":"Goldberg","year":"1994","journal-title":"Orthopedics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/s41536-019-0083-6","article-title":"Mesenchymal stem cell perspective: Cell biology to clinical progress","volume":"4","author":"Pittenger","year":"2019","journal-title":"npj Regen. 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