{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:08:42Z","timestamp":1788944922712,"version":"build-2803163510"},"reference-count":40,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270721"],"award-info":[{"award-number":["82270721"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["82270721"],"award-info":[{"award-number":["82270721"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["7252169"],"award-info":[{"award-number":["7252169"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["7244413"],"award-info":[{"award-number":["7244413"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Medical adhesives have been extensively applied for skin wound repair and hemostasis, while the dynamically humid and complex intraperitoneal environment imposes stringent requirements on tissue adhesives. Few studies have reported the application of biological adhesives for bladder defect repair in urological practice. The GSF composite hydrogel adhesive, composed of gelatin methacryloyl (GelMA), silk fibroin methacryloyl (SilMA), and polyether F127 diacrylate (F127DA), was previously fabricated with favorable injectability, appropriate viscosity and superior mechanical toughness. To further confirm the repair efficacy, GSF was characterized in vitro for the adhesive properties and structural stability under dynamically humid conditions. Corresponding in vivo experiments were conducted on rat models with graded bladder rupture and partial bladder defects to further assess the repair performance of GSF. Results showed that GSF hydrogel retained robust interfacial adhesion and intact structural stability for 8 days under dynamically simulated humid intraperitoneal conditions and cyclic urine filling-emptying environments. GSF exhibited rapid bonding capacity and effectively shortened the operation time. Under moderate bladder injury conditions, the wound closure time of the GSF group was only 28.3% of that of the suture group in cystotomy experiment and 34.5% in cystectomy experiment. It achieved satisfactory repair of mild-to-moderate bladder ruptures and partial bladder defects. Postoperative histological and functional evaluations confirmed that GSF hydrogel facilitated bladder structural reconstruction and functional recovery. These findings highlight the significant potential of GSF hydrogel adhesives for repairing and regenerating injured bladders. Future applications of GSF will extend to adhesive repairs of organs within the humidity conditions of the abdominal cavity.<\/jats:p>","DOI":"10.3390\/bioengineering13091047","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T08:05:43Z","timestamp":1788941143000},"page":"1047","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of Photocrosslinkable Wet-Adhesive Composite Hydrogel Adhesive in Bladder Injury Repair"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6612-3191","authenticated-orcid":false,"given":"Kaipeng","family":"Bi","sequence":"first","affiliation":[{"name":"School of Medicine, Nankai University, Tianjin 300071, China"},{"name":"Senior Department of Urology, Chinese PLA General Hospital, Beijing 100039, 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Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6489","DOI":"10.1007\/s00464-020-08142-x","article-title":"Comparison of gastrojejunostomy techniques and anastomotic complications: A systematic literature review","volume":"35","author":"Fakas","year":"2021","journal-title":"Surg. Endosc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s00384-023-04506-6","article-title":"Impact of powered circular stapler on anastomotic leak after anastomosis to the rectum: A propensity score matched study","volume":"38","author":"Vignali","year":"2023","journal-title":"Int. J. Colorectal Dis."},{"key":"ref_14","first-page":"52","article-title":"An adhesive and resilient hydrogel for the sealing and treatment of gastric perforation","volume":"14","author":"Chen","year":"2022","journal-title":"Bioact. Mater."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6169","DOI":"10.1039\/D1BM00761K","article-title":"Bi-layer silk fibroin skeleton and bladder acellular matrix hydrogel encapsulating adipose-derived stem cells for bladder reconstruction","volume":"9","author":"Xiao","year":"2021","journal-title":"Biomater. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"100485","DOI":"10.1016\/j.mtbio.2022.100485","article-title":"An all-silk-derived bilayer hydrogel for osteochondral tissue engineering","volume":"17","author":"Jiang","year":"2022","journal-title":"Mater. Today Bio"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.actbio.2021.04.010","article-title":"A 3D-printed PRP-GelMA hydrogel promotes osteochondral regeneration through M2 macrophage polarization in a rabbit model","volume":"128","author":"Jiang","year":"2021","journal-title":"Acta Biomater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5307","DOI":"10.1021\/acsbiomaterials.2c01127","article-title":"Cell infiltrative inner connected porous hydrogel improves neural stem cell migration and differentiation for functional repair of spinal cord injury","volume":"8","author":"Shi","year":"2022","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.biomaterials.2015.08.045","article-title":"Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels","volume":"73","author":"Yue","year":"2015","journal-title":"Biomaterials"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"112151","DOI":"10.1016\/j.compositesb.2025.112151","article-title":"Micelle-facilitated gelation kinetics and viscoelasticity of dynamic hyaluronan hydrogels for bioprinting of mimetic constructs and tissue repair","volume":"294","author":"Zhang","year":"2025","journal-title":"Compos. Part B"},{"key":"ref_21","first-page":"409","article-title":"Double-network hydrogel enhanced by SS31-loaded mesoporous polydopamine nanoparticles: Symphonic collaboration of near-infrared photothermal antibacterial effect and mitochondrial maintenance for full-thickness wound healing in diabetes mellitus","volume":"27","author":"Deng","year":"2023","journal-title":"Bioact. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"37301","DOI":"10.1021\/acsami.2c06865","article-title":"Development of enzymatic-resistant and compliant decellularized extracellular matrixes via aliphatic chain modification for bladder tissue engineering","volume":"14","author":"Sharma","year":"2022","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e2100446","DOI":"10.1002\/advs.202100446","article-title":"Toward a Better Regeneration through implant-mediated immunomodulation: Harnessing the immune responses","volume":"8","author":"Zhang","year":"2021","journal-title":"Adv. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.actbio.2022.11.029","article-title":"Porous composite hydrogels with improved MSC survival for robust epithelial sealing around implants and M2 macrophage polarization","volume":"157","author":"Li","year":"2023","journal-title":"Acta Biomater."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"100550","DOI":"10.1016\/j.mtbio.2023.100550","article-title":"Bioprinting a skin patch with dual-crosslinked gelatin (GelMA) and silk fibroin (SilMA): An approach to accelerating cutaneous wound healing","volume":"18","author":"Xu","year":"2023","journal-title":"Mater. Today Bio"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e2309562","DOI":"10.1002\/advs.202309562","article-title":"Harnessing mechanical stress with viscoelastic biomaterials for periodontal ligament regeneration","volume":"11","author":"Zhang","year":"2024","journal-title":"Adv. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1007\/s00345-024-05335-0","article-title":"Use of omentum during robotic-assisted reconstructive urological surgery: A systematic review of the current literature","volume":"42","author":"Anderson","year":"2024","journal-title":"World J. Urol."},{"key":"ref_28","first-page":"387","article-title":"Prevention strategies of postoperative adhesion in soft tissues by applying biomaterials: Based on the mechanisms of occurrence and development of adhesions","volume":"26","author":"Liao","year":"2023","journal-title":"Bioact. Mater."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e10745","DOI":"10.1002\/btm2.10745","article-title":"The BAM-GelMA-ADSCs bilayer patch promotes tissue regeneration and functional recovery after large-area bladder defects in beagles","volume":"10","author":"An","year":"2025","journal-title":"Bioeng. Transl. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1000170","DOI":"10.4172\/2157-7552.1000170","article-title":"A Review on Bladder Wound Healing after Mechanical Injury","volume":"7","author":"Larsson","year":"2016","journal-title":"J. Tissue Sci. Eng."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.actbio.2024.05.036","article-title":"Shape-fixing hydrogel promotes scarless healing of wounds under tension","volume":"183","author":"Fu","year":"2024","journal-title":"Acta Biomater."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.actbio.2022.07.042","article-title":"Mechanomodulatory biomaterials prospects in scar prevention and treatment","volume":"150","author":"Fernandes","year":"2022","journal-title":"Acta Biomater."},{"key":"ref_33","first-page":"1827","article-title":"Procyanidins-crosslinked small intestine submucosa: A bladder patch promotes smooth muscle regeneration and bladder function restoration in a rabbit model","volume":"6","author":"Zhang","year":"2021","journal-title":"Bioact. Mater."},{"key":"ref_34","first-page":"553","article-title":"A biodegradable microgrooved and tissue mechanocompatible citrate-based scaffold improves bladder tissue regeneration","volume":"41","author":"Goedegebuure","year":"2024","journal-title":"Bioact. Mater."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.actbio.2022.09.006","article-title":"Injectable adhesive self-healing biocompatible hydrogel for haemostasis, wound healing, and postoperative tissue adhesion prevention in nephron-sparing surgery","volume":"152","author":"Wan","year":"2022","journal-title":"Acta Biomater."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e2001341","DOI":"10.1002\/adhm.202001341","article-title":"Recent Advances in injectable hydrogels for controlled and local drug delivery","volume":"10","author":"Rizzo","year":"2021","journal-title":"Adv. Healthc. Mater."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s12951-024-02322-w","article-title":"Fabrication of levofloxacin-loaded porcine acellular dermal matrix hydrogel and functional assessment in urinary tract infection","volume":"22","author":"Yang","year":"2024","journal-title":"J. Nanobiotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"rbae083","DOI":"10.1093\/rb\/rbae083","article-title":"A bilayer bioengineered patch with sequential dual-growth factor release to promote vascularization in bladder reconstruction","volume":"11","author":"Zhao","year":"2024","journal-title":"Regen. Biomater."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"7590","DOI":"10.1038\/s41598-026-36959-4","article-title":"Biological welding enables rapid and efficient bladder cystotomy closure and reveals the underlying repair mechanism","volume":"16","author":"Zeng","year":"2026","journal-title":"Sci. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"97","DOI":"10.3892\/etm.2025.12847","article-title":"Tissue adhesion after surgical interventions (Review)","volume":"29","author":"Sturm","year":"2025","journal-title":"Exp. Ther. 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