{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T05:02:32Z","timestamp":1789448552127,"version":"build-2803163510"},"reference-count":61,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["NRF-2022R1F1A1073895"],"award-info":[{"award-number":["NRF-2022R1F1A1073895"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"award":["NRF-2022R1F1A1073895"],"award-info":[{"award-number":["NRF-2022R1F1A1073895"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Asan Institute for Life Sciences","award":["2025IL0022"],"award-info":[{"award-number":["2025IL0022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Allergic eye disease (AED), including allergic conjunctivitis, is a prevalent immune-mediated disorder of the ocular surface characterized by itching, redness, tearing, and ocular discomfort. Although olopatadine (OLO) is widely used as an antihistamine and mast cell stabilizer, its broader effects on immune-mediated inflammation, neuropeptide-associated responses, and epithelial\u2013mucosal changes in AED remain incompletely understood. This study investigated the multifaceted effects of OLO in an ovalbumin (OVA)-induced AED mouse model. AED was induced in BALB\/c mice by OVA sensitization and topical challenge, followed by OLO treatment during the challenge period. Clinical severity, scratching behavior, serum IgE, inflammatory cell infiltration (PMNs), mast cell degranulation, cytokine expression (IL-4, IL-5, IL-6, TNF-\u03b1, and IL-1\u03b2), macrophage infiltration (F4\/80), iNOS expression, TRPV1, SP, and CGRP expression, ZO-1 expression, goblet cell density, mucin expression (MUC5AC), dysregulated cell proliferation (Ki67), and apoptotic cell death (TUNEL) were assessed. OVA challenge induced marked allergic responses, including elevated clinical scores, increased scratching behavior, elevated serum IgE, mast cell degranulation, inflammatory cell infiltration, and upregulation of Th2- and pro-inflammatory cytokines. In addition, OLO reduced TRPV1, SP, and CGRP expression, preserved ZO-1 expression and goblet cell density, and reduced excessive epithelial cell proliferation. Notably, OLO demonstrated multifaceted protective effects in AED, providing preclinical insights into its effects in experimental AED while acknowledging its established clinical use in humans.<\/jats:p>","DOI":"10.3390\/bioengineering13091060","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T08:10:23Z","timestamp":1789373423000},"page":"1060","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Olopatadine Exerts Multifaceted Protective Effects in an Ovalbumin-Induced Allergic Eye Disease Mouse Model"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0503-8900","authenticated-orcid":false,"given":"Basanta","family":"Bhujel","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul 05505, Republic of Korea"},{"name":"Department of Medical Science, University of Ulsan Graduate School, Seoul 05505, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2598-9375","authenticated-orcid":false,"given":"Kyu Sang","family":"Eah","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul 05505, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Soon Suk","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul 05505, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Ho Seok","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul 05505, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Hun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul 05505, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8571-8350","authenticated-orcid":false,"given":"Jae-Yong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Asan Medical Center, College of Medicine, University of Ulsan, Seoul 05505, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1007\/s11882-002-0062-6","article-title":"Pathophysiology of ocular allergy: The roles of conjunctival mast cells and epithelial cells","volume":"2","author":"Stahl","year":"2002","journal-title":"Curr. Allergy Asthma Rep."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Tariq, F. (2024). Allergic conjunctivitis: Review of current types, treatments, and trends. Life, 14.","DOI":"10.3390\/life14060650"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1111\/all.13786","article-title":"Management of ocular allergy","volume":"74","author":"Leonardi","year":"2019","journal-title":"Allergy"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.jaci.2004.10.042","article-title":"Allergic conjunctivitis: Update on pathophysiology and prospects for future treatment","volume":"115","author":"Ono","year":"2005","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Chigbu, D.I., Karbach, N.J., Abu, S.L., and Hehar, N.K. (2024). Cytokines in allergic conjunctivitis: Unraveling their pathophysiological roles. Life, 14.","DOI":"10.3390\/life14030350"},{"key":"ref_6","first-page":"CD009566","article-title":"Topical antihistamines and mast cell stabilisers for treating seasonal and perennial allergic conjunctivitis","volume":"2015","author":"Castillo","year":"2015","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1517\/14656561003694643","article-title":"Olopatadine: A drug for allergic conjunctivitis targeting the mast cell","volume":"11","author":"Leonardi","year":"2010","journal-title":"Expert Opin. Pharmacother."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1111\/all.15174","article-title":"Epithelial barrier dysfunction in ocular allergy","volume":"77","author":"Singh","year":"2022","journal-title":"Allergy"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/j.jaci.2023.03.013","article-title":"Neuroimmune interactions in atopic and allergic contact dermatitis","volume":"151","author":"Liu","year":"2023","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1007\/s11882-024-01150-0","article-title":"Allergic Conjunctivitis Management: Update on Ophthalmic Solutions","volume":"24","author":"Leonardi","year":"2024","journal-title":"Curr. Allergy Asthma Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1365-2249.2008.03716.x","article-title":"Allergy and the eye","volume":"153","author":"Leonardi","year":"2008","journal-title":"Clin. Exp. Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.jtos.2017.05.002","article-title":"TFOS DEWS II pain and sensation report","volume":"15","author":"Belmonte","year":"2017","journal-title":"Ocul. Surf."},{"key":"ref_13","first-page":"77","article-title":"Comparison of effects of alcaftadine and olopatadine on conjunctival epithelium and eosinophil recruitment in a murine model of allergic conjunctivitis","volume":"5","author":"Ono","year":"2011","journal-title":"Drug Des. Devel. Ther."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"525","DOI":"10.2147\/OPTH.S3294","article-title":"Treatment of allergic conjunctivitis with olopatadine hydrochloride eye drops","volume":"2","author":"Uchio","year":"2008","journal-title":"Clin. Ophthalmol."},{"key":"ref_15","first-page":"809","article-title":"Properties of olopatadine hydrochloride, a new antiallergic\/antihistaminic drug","volume":"54","author":"Ohmori","year":"2004","journal-title":"Arzneimittelforschung"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Zhao, Z., Liang, M., He, H., Wang, X., Zhu, C., Li, L., Liu, B., Zong, R., Jin, Q., and Wu, H. (2022). Ovalbumin-Induced Allergic Inflammation Diminishes Cross-Linked Collagen Structures in an Experimental Rabbit Model of Corneal Cross-Linking. Front. Med., 9.","DOI":"10.3389\/fmed.2022.762730"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1167\/iovs.15-18457","article-title":"Topical application of interleukin-28A attenuates allergic conjunctivitis in an ovalbumin-induced mouse model","volume":"57","author":"Chen","year":"2016","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_18","first-page":"1966","article-title":"Curcumin suppresses ovalbumin-induced allergic conjunctivitis","volume":"18","author":"Chung","year":"2012","journal-title":"Mol. Vis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/imr.12549","article-title":"Modeling T(H) 2 responses and airway inflammation to understand fundamental mechanisms regulating the pathogenesis of asthma","volume":"278","author":"Foster","year":"2017","journal-title":"Immunol. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1242\/dmm.000323","article-title":"Mouse models of allergic asthma: Acute and chronic allergen challenge","volume":"1","author":"Nials","year":"2008","journal-title":"Dis. Model. Mech."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Kishimoto, T., Ishida, W., Nakajima, I., Fukuda, K., and Yamashiro, K. (2022). Aqueous-deficient dry eye exacerbates signs and symptoms of allergic conjunctivitis in mice. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23094918"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Reyes, N.J., Mathew, R., and Saban, D.R. (2018). Induction and characterization of the allergic eye disease mouse model. Type 2 Immunity: Methods and Protocols, Springer.","DOI":"10.1007\/978-1-4939-7896-0_5"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S1081-1206(10)62814-1","article-title":"Evaluation of olopatadine, a new ophthalmic antiallergic agent with dual activity, using the conjunctival allergen challenge model","volume":"81","author":"Abelson","year":"1998","journal-title":"Ann. Allergy Asthma Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3319","DOI":"10.3892\/mmr.2016.4937","article-title":"Treatment with olopatadine and naphazoline hydrochloride reduces allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF","volume":"13","author":"Quan","year":"2016","journal-title":"Mol. Med. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Bhujel, B., Oh, S., Hur, W., Lee, S., Chung, H.S., Lee, H., Park, J.H., and Kim, J.Y. (2024). Effect of Exposure to Particulate Matter on the Ocular Surface in an Experimental Allergic Eye Disease Mouse Model. Bioengineering, 11.","DOI":"10.3390\/bioengineering11050498"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"107225","DOI":"10.1016\/j.vascn.2022.107225","article-title":"A mouse model of allergic conjunctivitis permitting tear eosinophil quantification","volume":"118","author":"Ogura","year":"2022","journal-title":"J. Pharmacol. Toxicol. Methods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/1824-7288-39-18","article-title":"Allergic conjunctivitis: A comprehensive review of the literature","volume":"39","author":"Lionetti","year":"2013","journal-title":"Ital. J. Pediatr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1111\/pai.12197","article-title":"Vernal keratoconjunctivitis: A severe allergic eye disease with remodeling changes","volume":"25","author":"Vichyanond","year":"2014","journal-title":"Pediatr. Allergy Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1136\/bjo.82.7.797","article-title":"Impairment of ocular surface epithelium barrier function in patients with atopic dermatitis","volume":"82","author":"Yokoi","year":"1998","journal-title":"Br. J. Ophthalmol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"59","DOI":"10.4168\/aair.2022.14.1.59","article-title":"Particulate Matter Exposure Aggravates IL-17-Induced Eye and Nose Inflammation in an OVA\/Poly(I:C) Mouse Model","volume":"14","author":"Bae","year":"2022","journal-title":"Allergy Asthma Immunol. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1016\/j.immuni.2015.08.020","article-title":"Induction of Interleukin-9-Producing Mucosal Mast Cells Promotes Susceptibility to IgE-Mediated Experimental Food Allergy","volume":"43","author":"Chen","year":"2015","journal-title":"Immunity"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1710-1492-10-30","article-title":"Enhancement of OVA-induced murine lung eosinophilia by co-exposure to contamination levels of LPS in Asian sand dust and heated dust","volume":"10","author":"Ren","year":"2014","journal-title":"Allergy Asthma Clin. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1159\/000236002","article-title":"Participation of CD11b and F4\/80 molecules in the conjunctival eosinophilia of experimental allergic conjunctivitis","volume":"151","author":"Fukushima","year":"2010","journal-title":"Int. Arch. Allergy Immunol."},{"key":"ref_34","first-page":"1280","article-title":"Conjunctival macrophages act as antigen-presenting cells in the conjunctiva during the development of experimental allergic conjunctivitis","volume":"16","author":"Ishida","year":"2010","journal-title":"Mol. Vis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1097\/00003226-200001000-00017","article-title":"The role of nitric oxide in experimental allergic conjunctivitis","volume":"19","author":"Ko","year":"2000","journal-title":"Cornea"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1185\/030079905X56547","article-title":"Mast cell stabilization and anti-histamine effects of olopatadine ophthalmic solution: A review of pre-clinical and clinical research","volume":"21","author":"Rosenwasser","year":"2005","journal-title":"Curr. Med. Res. Opin."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S1350-9462(02)00006-X","article-title":"Vernal keratoconjunctivitis: Pathogenesis and treatment","volume":"21","author":"Leonardi","year":"2002","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/nm.2755","article-title":"IgE and mast cells in allergic disease","volume":"18","author":"Galli","year":"2012","journal-title":"Nat. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s10792-004-1818-x","article-title":"A review of the use of olopatadine in allergic conjunctivitis","volume":"25","author":"McGill","year":"2004","journal-title":"Int. Ophthalmol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/s11882-001-0051-1","article-title":"Overview of ocular allergy treatment","volume":"1","author":"Friedlaender","year":"2001","journal-title":"Curr. Allergy Asthma Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1097\/ACI.0b013e32830e6b17","article-title":"The role of neuromediators in ocular allergy","volume":"8","author":"Micera","year":"2008","journal-title":"Curr. Opin. Allergy Clin. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Puri, S., Kenyon, B.M., and Hamrah, P. (2022). Immunomodulatory Role of Neuropeptides in the Cornea. Biomedicines, 10.","DOI":"10.3390\/biomedicines10081985"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.it.2016.10.001","article-title":"Nociceptor Sensory Neuron-Immune Interactions in Pain and Inflammation","volume":"38","author":"Verri","year":"2017","journal-title":"Trends Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.jaci.2006.09.013","article-title":"The role of the nervous system in rhinitis","volume":"118","author":"Sarin","year":"2006","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.ejphar.2011.04.027","article-title":"C-fibers, but not the transient potential receptor vanilloid 1 (TRPV1), play a role in experimental allergic airway inflammation","volume":"662","author":"Rogerio","year":"2011","journal-title":"Eur. J. Pharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1016\/S0140-6736(19)32504-8","article-title":"Targeting calcitonin gene-related peptide: A new era in migraine therapy","volume":"394","author":"Charles","year":"2019","journal-title":"Lancet"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Hwang, D.D.-J., Lee, S.-J., Kim, J.-H., and Lee, S.-M. (2021). The role of neuropeptides in pathogenesis of dry eye. J. Clin. Med., 10.","DOI":"10.3390\/jcm10184248"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"L941","DOI":"10.1152\/ajplung.00366.2011","article-title":"TRPV1 induction in airway vagal low-threshold mechanosensory neurons by allergen challenge and neurotrophic factors","volume":"302","author":"Lieu","year":"2012","journal-title":"Am. J. Physiol.-Lung Cell. Mol. Physiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1097\/ACI.0000000000000934","article-title":"Ocular surface itch and pain: Key differences and similarities between the two sensations","volume":"23","author":"Raolji","year":"2023","journal-title":"Curr. Opin. Allergy Clin. Immunol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"349","DOI":"10.18176\/jiaci.0320","article-title":"Neuropathic pain and itch mechanisms underlying allergic conjunctivitis","volume":"29","author":"Kuruvilla","year":"2019","journal-title":"J. Investig. Allergol. Clin. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2353","DOI":"10.1016\/j.pain.2013.07.012","article-title":"Changes in sensory activity of ocular surface sensory nerves during allergic keratoconjunctivitis","volume":"154","author":"Acosta","year":"2013","journal-title":"PAIN\u00ae"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1146\/annurev-cellbio-101011-155833","article-title":"TRP channels and pain","volume":"29","author":"Julius","year":"2013","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/S1542-0124(12)70112-X","article-title":"Nerves and sensations from the eye surface","volume":"2","author":"Belmonte","year":"2004","journal-title":"Ocul. Surf."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.brainres.2006.02.035","article-title":"Peripheral and central distribution of TRPV1, substance P and CGRP of rat corneal neurons","volume":"1085","author":"Murata","year":"2006","journal-title":"Brain Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1097\/ACI.0b013e3283645899","article-title":"The ocular surface epithelial barrier and other mechanisms of mucosal protection: From allergy to infectious diseases","volume":"13","author":"Mauris","year":"2013","journal-title":"Curr. Opin. Allergy Clin. Immunol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"971","DOI":"10.3109\/02713683.2012.700756","article-title":"Structural and functional alteration of corneal epithelial barrier under inflammatory conditions","volume":"37","author":"Schulze","year":"2012","journal-title":"Curr. Eye Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1111\/j.1398-9995.2006.01207.x","article-title":"Reduced structural proteins in the conjunctival epithelium in allergic eye disease","volume":"61","author":"Hughes","year":"2006","journal-title":"Allergy"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1038\/s41467-023-37101-y","article-title":"The protective role of conjunctival goblet cell mucin sialylation","volume":"14","author":"Matsuzawa","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1016\/S0149-2918(02)80035-2","article-title":"Changes in tear function and the ocular surface after topical olopatadine treatment for allergic conjunctivitis: An open-label study","volume":"24","author":"Dogru","year":"2002","journal-title":"Clin. Ther."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1097\/ACI.0000000000000473","article-title":"Topical antihistamines, mast cell stabilizers, and dual-action agents in ocular allergy: Current trends","volume":"18","author":"Solomon","year":"2018","journal-title":"Curr. Opin. Allergy Clin. Immunol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1097\/ICO.0000000000001982","article-title":"Use of Topical Steroids in Conjunctivitis: A Review of the Evidence","volume":"38","author":"Holland","year":"2019","journal-title":"Cornea"}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/13\/9\/1060\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T04:17:37Z","timestamp":1789445857000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/13\/9\/1060"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,12]]},"references-count":61,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["bioengineering13091060"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering13091060","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,12]]}}}