{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T12:25:21Z","timestamp":1793535921615,"version":"4.5.0"},"reference-count":43,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,11,5]],"date-time":"2021-11-05T00:00:00Z","timestamp":1636070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Anaerobic digestion for the valorization of organic wastes into biogas is gaining worldwide interest. Nonetheless, the sizing of the biogas plant units require knowledge of the quantity of feedstock, and their associated methane potentials, estimated widely by Biochemical Methane Potential (BMP) tests. Discrepancies exist among laboratories due to variability of protocols adopted and operational factors used. The aim of this study is to verify the influence of some operational factors (e.g., analysis frequency, trace elements and vitamins solution addition and flushing gas), feedstock conservation and the source of inoculum on BMP. Among the operational parameters tested on cellulose degradation, only the type of gas used for flushing headspace of BMP assays had shown a significant influence on methane yields from cellulose. Methane yields of 344 \u00b1 6 NL CH4 kg\u22121 VS and 321 \u00b1 10 NL CH4 kg\u22121 VS obtained from assays flushed with pure N2 and N2\/CO2 (60\/40 v\/v). The origin of inoculum (fed in co-digestion) only significantly affected the methane yields for straw, 253 \u00b1 3 and 333 \u00b1 3 NL CH4 kg\u22121 VS. Finally, freezing\/thawing cycle effect depended of the substrate (tested on biowaste, manure, straw and WWTP sludge) with a possible effect of water content substrate.<\/jats:p>","DOI":"10.3390\/bioengineering8110176","type":"journal-article","created":{"date-parts":[[2021,11,7]],"date-time":"2021-11-07T20:41:14Z","timestamp":1636317674000},"page":"176","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Impact of Operational Factors, Inoculum Origin, and Feedstock Preservation on the Biochemical Methane Potential"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6131-1449","authenticated-orcid":false,"given":"Audrey","family":"Lallement","sequence":"first","affiliation":[{"name":"APESA, P\u00f4le Valorisation, Cap Ecologia, Avenue Fr\u00e9deric Joliot Curie, 64230 Lescar, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Siaud","sequence":"additional","affiliation":[{"name":"APESA, P\u00f4le Valorisation, Cap Ecologia, Avenue Fr\u00e9deric Joliot Curie, 64230 Lescar, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Peyrelasse","sequence":"additional","affiliation":[{"name":"APESA, P\u00f4le Valorisation, Cap Ecologia, Avenue Fr\u00e9deric Joliot Curie, 64230 Lescar, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasad","family":"Kaparaju","sequence":"additional","affiliation":[{"name":"School of Engineering and Built Environment, Nathan Campus, Griffith University, Brisbane, QLD 4111, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blandine","family":"Schraauwers","sequence":"additional","affiliation":[{"name":"APESA, P\u00f4le Valorisation, Cap Ecologia, Avenue Fr\u00e9deric Joliot Curie, 64230 Lescar, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Maunas","sequence":"additional","affiliation":[{"name":"APESA, P\u00f4le Valorisation, Cap Ecologia, Avenue Fr\u00e9deric Joliot Curie, 64230 Lescar, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Monlau","sequence":"additional","affiliation":[{"name":"APESA, P\u00f4le Valorisation, Cap Ecologia, Avenue Fr\u00e9deric Joliot Curie, 64230 Lescar, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.3389\/fenrg.2017.00012","article-title":"Methane production of full-scale anaerobic digestion plants calculated from substrate\u2019s biomethane potentials compares well with the one measured on-site","volume":"5","author":"Holliger","year":"2017","journal-title":"Front. 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