{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:19:40Z","timestamp":1790839180428,"version":"4.1.0"},"reference-count":36,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T00:00:00Z","timestamp":1658102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Culture, Research, and Technology","award":["1724\/UN1\/DITLIT\/Dit-Lit\/PT.01.03\/2022"],"award-info":[{"award-number":["1724\/UN1\/DITLIT\/Dit-Lit\/PT.01.03\/2022"]}]},{"name":"Ministry of Education, Culture, Research, and Technology","award":["482\/UN1.P.III\/KPT\/HUKOR\/2022"],"award-info":[{"award-number":["482\/UN1.P.III\/KPT\/HUKOR\/2022"]}]},{"name":"Ministry of Education, Culture, Research, and Technology","award":["KET-1890\/LPDP.4\/2019"],"award-info":[{"award-number":["KET-1890\/LPDP.4\/2019"]}]},{"name":"Directorate of Research Universitas Gadjah Mada","award":["1724\/UN1\/DITLIT\/Dit-Lit\/PT.01.03\/2022"],"award-info":[{"award-number":["1724\/UN1\/DITLIT\/Dit-Lit\/PT.01.03\/2022"]}]},{"name":"Directorate of Research Universitas Gadjah Mada","award":["482\/UN1.P.III\/KPT\/HUKOR\/2022"],"award-info":[{"award-number":["482\/UN1.P.III\/KPT\/HUKOR\/2022"]}]},{"name":"Directorate of Research Universitas Gadjah Mada","award":["KET-1890\/LPDP.4\/2019"],"award-info":[{"award-number":["KET-1890\/LPDP.4\/2019"]}]},{"name":"Indonesian Endowment Fund for Education","award":["1724\/UN1\/DITLIT\/Dit-Lit\/PT.01.03\/2022"],"award-info":[{"award-number":["1724\/UN1\/DITLIT\/Dit-Lit\/PT.01.03\/2022"]}]},{"name":"Indonesian Endowment Fund for Education","award":["482\/UN1.P.III\/KPT\/HUKOR\/2022"],"award-info":[{"award-number":["482\/UN1.P.III\/KPT\/HUKOR\/2022"]}]},{"name":"Indonesian Endowment Fund for Education","award":["KET-1890\/LPDP.4\/2019"],"award-info":[{"award-number":["KET-1890\/LPDP.4\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>In this work, carbonated hydroxyapatite\/titanium alloy (CHA\/Ti) and carbonated hydroxyapatite\/honeycomb\/titanium alloy (CHA\/HCB\/Ti) plates were coated using the electrophoretic deposition dip coating (EP2D) method. Analysis of cell viability and surface morphology of CHA\/Ti and CHA\/HCB\/Ti coatings were carried out using the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and scanning electron microscopy (SEM), respectively. In a previous study, the thickness and average compressive strength values for the CHA\/Ti and CHA\/HCB\/Ti plates were about 63\u201389 \u03bcm and 54\u201375 MPa, respectively. The result for thickness and compressive strength in this research followed the thickness and compressive strength parameters for coating in bone implants. In this work, the cell viability for incubation times during 24 h and 48 h of CHA\/Ti plates is demonstrably superior to that of CHA\/HCB\/Ti plates, respectively, where the cell viability for CHA\/Ti plates increased to ((67 \u00b1 2)%) after incubation for 48 h. According to the one-way analysis of variance (ANOVA), the p-value was &lt;0.05, indicating a significant difference in the average cell viability value across the three groups. Furthermore, the surface of CHA\/Ti is not changed after the coating process. These results will yield many positive biomedical applications, especially in bone implants. Overall, CHA\/Ti and CHA\/HCB\/Ti plates can be considered candidates for biomedical applications based on an analysis of surface morphology and cell viability.<\/jats:p>","DOI":"10.3390\/bioengineering9070325","type":"journal-article","created":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T11:32:23Z","timestamp":1658143943000},"page":"325","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Cell Viability Assay and Surface Morphology Analysis of Carbonated Hydroxyapatite\/Honeycomb\/Titanium Alloy Coatings for Bone Implant Applications"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0056-3252","authenticated-orcid":false,"given":"Mona","family":"Sari","sequence":"first","affiliation":[{"name":"Department of Physics, Faculty of Mathematics and Natural Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Chotimah","sequence":"additional","affiliation":[{"name":"Department of Physics, Faculty of Mathematics and Natural Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9957-4247","authenticated-orcid":false,"given":"Ika Dewi","family":"Ana","sequence":"additional","affiliation":[{"name":"Department of Dental Biomedical Sciences, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9104-9333","authenticated-orcid":false,"given":"Yusril","family":"Yusuf","sequence":"additional","affiliation":[{"name":"Department of Physics, Faculty of Mathematics and Natural Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1016\/j.jmrt.2021.03.009","article-title":"Structural, morphological and biological evaluations of cerium incorporated hydroxyapatite sol gel coatings on Ti-6Al-4V for orthopaedic applications","volume":"12","author":"Priyadarshini","year":"2021","journal-title":"J. Mater. Res. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4160","DOI":"10.1016\/j.msec.2013.06.010","article-title":"Electrophoretic bilayer deposition of zirconia and reinforced bioglass system on Ti6Al4V for implant applications: An in vitro investigation","volume":"33","author":"Ananth","year":"2013","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"137801","DOI":"10.1016\/j.tsf.2020.137801","article-title":"Electrophoretic deposition of hydroxyapatite-iron oxide-chitosan composite coatings on Ti-13Nb-13Zr alloy for biomedical applications","volume":"697","author":"Singh","year":"2020","journal-title":"Thin Solid Films"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.molstruc.2017.08.070","article-title":"Effect of sintering temperatures on the in vitro bioactivity, molecular structure, and mechanical properties of titanium\/carbonated hydroxyapatite nano biocomposites","volume":"1150","author":"Youness","year":"2017","journal-title":"J. Mol. Struct."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1007\/s10856-015-5565-5","article-title":"Compressive mechanical compatibility of anisotropic porous Ti6Al4V alloys in the range of physiological strain rate for cortical bone implant applications","volume":"26","author":"Li","year":"2015","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2714","DOI":"10.1039\/D0BM00269K","article-title":"Influence of the mechanical properties of biomaterials on degradability, cell behaviors and signaling pathways: Current progress and challenges","volume":"8","author":"Wang","year":"2020","journal-title":"Biomater. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Sari, M., Kristianto, N.A., Ana, I.D., and Yusuf, Y. (2021). Carbonated Hydroxyapatite-based honeycomb scaffold coatings on a titanium alloy for bone implant application\u2014Physicochemical and mechanical properties Analysis. Coatings, 11.","DOI":"10.3390\/coatings11080941"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"126594","DOI":"10.1016\/j.surfcoat.2020.126594","article-title":"In vitro study: Bond strength, electrochemical, and biocompatibility evaluations of TiO2\/Al2O3 reinforced hydroxyapatite sol-gel coatings on 316L SS","volume":"405","author":"Ahmadi","year":"2021","journal-title":"Surf. Coat. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.surfcoat.2011.06.034","article-title":"Electrophoretic deposition of hydroxyapatite coatings on titanium from dimethylformamide suspensions","volume":"206","author":"Abdeltawab","year":"2011","journal-title":"Surf. Coat. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/18479804211032851","article-title":"Nano-carbonated hydroxyapatite precipitation from abalone shell (Haliotis asinina) waste as the bioceramics candidate for bone tissue engineering","volume":"11","author":"Permatasari","year":"2021","journal-title":"Nanomater. Nanotechnol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"102135","DOI":"10.1016\/j.mtcomm.2021.102135","article-title":"Porous structure of bioceramics carbonated hydroxyapatite-based honeycomb scaffold for bone tissue engineering","volume":"26","author":"Sari","year":"2021","journal-title":"Mater. Today Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.pmatsci.2019.01.001","article-title":"Electrophoretic deposition of chitosan-based composite coatings for biomedical applications: A review","volume":"103","author":"Avcu","year":"2019","journal-title":"Prog. Mater. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/13588265.2018.1480471","article-title":"Crushing behavior of honeycomb structure: A review","volume":"24","author":"Thomas","year":"2019","journal-title":"Int. J. Crashworthiness"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.foodchem.2017.08.083","article-title":"Honey is cytotoxic towards prostate cancer cells but interacts with the MTT reagent: Considerations for the choice of cell viability assay","volume":"241","author":"Abel","year":"2018","journal-title":"Food.Chem."},{"key":"ref_15","first-page":"11","article-title":"Synthesis and characterization of hydroxyapatite layer on cobalt alloys through dip coating method as a prosthetic bone implant candidate","volume":"7","author":"Aminatun","year":"2015","journal-title":"J. Optoelectron. Biomed. Mater."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"11820","DOI":"10.1016\/j.ceramint.2017.06.026","article-title":"Electrophoretic deposition (EPD) of nanohydroxyapatite-nanosilver coatings on Ti13Zr13Nb alloy","volume":"43","author":"Bartmanski","year":"2017","journal-title":"Ceram. Int."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.matlet.2018.09.066","article-title":"Electrophoretic coatings of hydroxyapatite with various nanocrystal shapes","volume":"234","author":"Patel","year":"2019","journal-title":"Mater. Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.jtice.2021.02.006","article-title":"Effects of pH on inhibitor-doped hybrid protective sol-gel coatings on the copper electrode surface","volume":"119","author":"Balaji","year":"2021","journal-title":"J. Taiwan Inst. Chem. Eng."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"127098","DOI":"10.1016\/j.surfcoat.2021.127098","article-title":"Physical principles of radio-frequency magnetron sputter deposition of calcium-phosphate-based coating with tailored properties","volume":"413","author":"Surmenev","year":"2021","journal-title":"Surf. Coat. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1007\/s11666-016-0421-9","article-title":"Plasma-sprayed hydroxylapatite-based coatings: Chemical, mechanical, microstructural, and biomedical properties","volume":"25","author":"Heimann","year":"2016","journal-title":"J. Therm. Spray Technol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.jmbbm.2018.01.005","article-title":"Characterization and mechanical behavior of reinforced hydroxyapatite coatings deposited by vacuum plasma spray on SS-316L alloy","volume":"79","author":"Singh","year":"2018","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"336","DOI":"10.4028\/www.scientific.net\/KEM.840.336","article-title":"Stainless steel 316 L metal coating with capiz shell hydroxyapatite using electrophoretic deposition method as bone implant candidate","volume":"840","author":"Kriswanto","year":"2020","journal-title":"Key Eng. Mater."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.jascer.2014.05.003","article-title":"Fabrication, bioactivity, in vitro cytotoxicity and cell viability of cryo-treated nanohydroxyapatite\u2014gelatin\u2013polyvinyl alcohol macroporous scaffold","volume":"2","author":"Swain","year":"2014","journal-title":"J. Asian. Ceram. Soc."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Sari, M., Suciati, T., Sari, Y.W., and Yusuf, Y. (2021). Porous carbonated hydroxyapatite-based paraffin wax nanocomposite scaffold for bone tissue engineering: A physicochemical properties and cell viability assay analysis. Coatings, 11.","DOI":"10.3390\/coatings11101189"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"68","DOI":"10.4012\/dmj.2021-102","article-title":"Development of a hydroxyapatite nanoparticle-based gel for enamel remineralization\u2014A physicochemical properties and cell viability assay analysis","volume":"41","author":"Sari","year":"2022","journal-title":"Dent. Mater. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"24134","DOI":"10.1038\/srep24134","article-title":"A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration","volume":"6","author":"Cheng","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3643","DOI":"10.2147\/IJN.S159989","article-title":"Evaluation of highly carbonated hydroxyapatite bioceramic implant coatings with hierarchical micro-\/nanorod topography optimized for osseointegration","volume":"13","author":"Li","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5560891","DOI":"10.1155\/2021\/5560891","article-title":"Bovine hydroxyapatite-based bone scaffold with gentamicin accelerates vascularization and remodeling of bone defect","volume":"2021","author":"Budiatin","year":"2021","journal-title":"Int. J. Biomater."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.actbio.2009.07.038","article-title":"Characterization of calcium phosphate cements modified by addition of amorphous calcium phosphate","volume":"6","author":"Pieters","year":"2010","journal-title":"Acta Biomater."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"153038","DOI":"10.1016\/j.jallcom.2019.153038","article-title":"Triggering the hydroxyapatite deposition on the surface of PEO-coated Tie6Ale4V alloy via the dual incorporation of Zn and Mg ions","volume":"819","author":"Kaseem","year":"2020","journal-title":"J. Alloys Compd."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"8789","DOI":"10.1016\/j.ceramint.2016.02.120","article-title":"Effects of alumina nanoparticles concentration on microstructure and corrosion behavior of coatings formed on titanium substrate via PEO process","volume":"42","author":"Sarbishei","year":"2016","journal-title":"Ceram. Int."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1186\/1754-6834-6-95","article-title":"Transcriptome response to alkane biofuels in Saccharomyces cerevisiae: Identification of efflux pumps involved in alkane tolerance","volume":"6","author":"Ling","year":"2013","journal-title":"Biotech. Biofuels"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1016\/j.tiv.2012.12.018","article-title":"In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells","volume":"27","author":"Hamzeh","year":"2013","journal-title":"Toxicol. In Vitro"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.apsusc.2015.11.086","article-title":"Formation mechanism and adhesive strength of a hydroxyapatite\/TiO2 composite coating on a titanium surface prepared by micro-arc oxidation","volume":"362","author":"Liu","year":"2016","journal-title":"Appl. Surf. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"108758","DOI":"10.1016\/j.matdes.2020.108758","article-title":"Y-doped TiO2 coating with superior bioactivity and antibacterial property prepared via plasma electrolytic oxidation","volume":"192","author":"Zhang","year":"2020","journal-title":"Mater. Des."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s10237-006-0030-5","article-title":"Effect of compressive strain on cell viability in statically loaded articular cartilage","volume":"5","author":"Torzilli","year":"2006","journal-title":"Biomech. Modell. Mechanobiol."}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/9\/7\/325\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:53:13Z","timestamp":1760140393000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/9\/7\/325"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,18]]},"references-count":36,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["bioengineering9070325"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering9070325","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,18]]}}}