{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:44:47Z","timestamp":1789188287593,"version":"build-2803163510"},"reference-count":47,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Skin diseases are commonly treated with antihistamines, antibiotics, laser therapy, topical medications, local vitamins, or steroids. Since conventional treatments for wound healing (skin allografts, amnion, xenografts, etc.) have disadvantages such as antigenicity of the donor tissue, risk of infection, or lack of basement membrane, skin tissue engineering has become a popular new approach. The current study presents the design and fabrication of a new wound-dressing material by the addition of Juglone (5-hydroxy-1,4-naphthoquinone) to a 25% Polycaprolactone (PCL) scaffold. Juglone (J) is a significant allelochemical found in walnut trees and, in this study is used as a bioactive material. The effects of different amounts of J (1.25, 2.5, 5, 7.5, and 10 mg) on the biocompatibility, mechanical, chemical, thermal, morphological, and antimicrobial properties of the 3D-printed 25% PCL scaffolds were investigated. The addition of J increased the pore diameter of the 25% PCL scaffold. The maximum pore size (290.72 \u00b1 14 \u00b5m) was observed for the highest amount of J (10 mg). The biocompatibility tests on the scaffolds demonstrated biocompatible behavior from the first day of incubation, the 25% PCL\/7.5 J scaffold having the highest viability value (118%) among all of the J-loaded scaffolds. Drug release of J into phosphate buffered saline (PBS) at pH 7.4 showed that J was completely released from all 25% PCL\/J scaffolds within 7 days of incubation.<\/jats:p>","DOI":"10.3390\/bioengineering9090427","type":"journal-article","created":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T21:25:18Z","timestamp":1661894718000},"page":"427","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["3D-Printed PCL Scaffolds Combined with Juglone for Skin Tissue Engineering"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2074-7219","authenticated-orcid":false,"given":"Musa","family":"Ayran","sequence":"first","affiliation":[{"name":"Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Istanbul 34722, Turkey"},{"name":"Department of Metallurgical and Materials Engineering, Institute of Pure and Applied Sciences, Marmara University, Istanbul 34722, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6457-9750","authenticated-orcid":false,"given":"Akif Yahya","family":"Dirican","sequence":"additional","affiliation":[{"name":"Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Istanbul 34722, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elif","family":"Saatcioglu","sequence":"additional","affiliation":[{"name":"Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Istanbul 34722, Turkey"},{"name":"Department of Metallurgical and Materials Engineering, Institute of Pure and Applied Sciences, Marmara University, Istanbul 34722, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8215-1504","authenticated-orcid":false,"given":"Songul","family":"Ulag","sequence":"additional","affiliation":[{"name":"Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Istanbul 34722, Turkey"},{"name":"Department of Metallurgical and Materials Engineering, Faculty of Technology, Marmara University, Istanbul 34722, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5594-1551","authenticated-orcid":false,"given":"Ali","family":"Sahin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Marmara University, Istanbul 34722, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burak","family":"Aksu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Marmara University, Istanbul 34722, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexa-Maria","family":"Croitoru","sequence":"additional","affiliation":[{"name":"Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Gh Polizu Street, 011061 Bucharest, Romania"},{"name":"National Centre for Micro- and Nanomaterials, University Politehnica of Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania"},{"name":"National Centre for Food Safety, University Politehnica of Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denisa","family":"Ficai","sequence":"additional","affiliation":[{"name":"Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Gh Polizu Street, 011061 Bucharest, Romania"},{"name":"National Centre for Micro- and Nanomaterials, University Politehnica of Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania"},{"name":"Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Gh Polizu Street, 011061 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9427-7574","authenticated-orcid":false,"given":"Oguzhan","family":"Gunduz","sequence":"additional","affiliation":[{"name":"Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Istanbul 34722, Turkey"},{"name":"Department of Metallurgical and Materials Engineering, Faculty of Technology, Marmara University, Istanbul 34722, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1777-0525","authenticated-orcid":false,"given":"Anton","family":"Ficai","sequence":"additional","affiliation":[{"name":"Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Gh Polizu Street, 011061 Bucharest, Romania"},{"name":"National Centre for Micro- and Nanomaterials, University Politehnica of Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania"},{"name":"National Centre for Food Safety, University Politehnica of Bucharest, Splaiul Independentei 313, 060042 Bucharest, Romania"},{"name":"Academy of Romanian Scientists, Ilfov Street 3, 50044 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,30]]},"reference":[{"key":"ref_1","unstructured":"McDonald, C. 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