{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:07:40Z","timestamp":1793117260105,"version":"4.5.0"},"reference-count":40,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T00:00:00Z","timestamp":1667865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Society for Blood Concentrates and Biomaterials, SBCB e.V.","award":["2021-21-1"],"award-info":[{"award-number":["2021-21-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioengineering"],"abstract":"<jats:p>Systematic evaluations regarding the influence of PRF in ridge sealing are still lacking. To the best of our knowledge, this is the first systemic randomized, controlled, clinical approach dealing with the potential of a systematic applied solid PRF on soft tissue socket healing of molar and premolar extraction sockets with evaluation for up to 90 days. Qualitative and quantitative image analysis showed that PRF contributed to a significantly faster ridge sealing, within the period of 7\u201310 days in both tooth types. This led to a visibly less contraction at the PRF-treated group sites at day 90. Patients\u2019 pain perception demonstrated no statistic significance between both groups (PRF vs. natural healing), but the patients in PRF group seemed to have had less pain throughout the observational period. It becomes evident that PRF is able to serve as a promotor of the secondary wound healing cascade. The guiding capacity of PRF accelerating the process of open ridge healing makes it possible to act as a natural growth factor drug delivery system, providing a more predictable guided open wound healing of the ridge with less contraction of the soft tissue, the latter being a key factor for the subsequent successful dental implantation and oral rehabilitation.<\/jats:p>","DOI":"10.3390\/bioengineering9110661","type":"journal-article","created":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T11:46:43Z","timestamp":1667908003000},"page":"661","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Solid PRF Serves as Basis for Guided Open Wound Healing of the Ridge after Tooth Extraction by Accelerating the Wound Healing Time Course\u2014A Prospective Parallel Arm Randomized Controlled Single Blind Trial"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7897-0854","authenticated-orcid":false,"given":"Shahram","family":"Ghanaati","sequence":"first","affiliation":[{"name":"FORM-Lab, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Goethe University, 60590 Frankfurt am Main, Germany"},{"name":"Department of Dental Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-027 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"\u015amieszek-Wilczewska","sequence":"additional","affiliation":[{"name":"Department of Dental Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-027 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Al-Maawi","sequence":"additional","affiliation":[{"name":"FORM-Lab, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Goethe University, 60590 Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Neff","sequence":"additional","affiliation":[{"name":"FORM-Lab, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Goethe University, 60590 Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Homayoun H.","family":"Zadeh","sequence":"additional","affiliation":[{"name":"FORM-Lab, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Goethe University, 60590 Frankfurt am Main, Germany"},{"name":"VISTA Institute for Therapeutic Innovations, Woodland Hills, CA 91367, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Sader","sequence":"additional","affiliation":[{"name":"FORM-Lab, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Goethe University, 60590 Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6799-8325","authenticated-orcid":false,"given":"Anja","family":"Heselich","sequence":"additional","affiliation":[{"name":"FORM-Lab, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Goethe University, 60590 Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James L.","family":"Rutkowski","sequence":"additional","affiliation":[{"name":"Restorative Dentistry, School of Dental Medicine, State University of New York, Buffalo, NY 14214, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.5051\/jpis.2020.50.2.97","article-title":"Clinical and radiographic assessment of narrow-diameter and regular-diameter implants in the anterior and posterior jaw: 2 to 6 years of follow-up","volume":"50","author":"Alrabiah","year":"2020","journal-title":"J. Periodontal Implant Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"6590202","DOI":"10.1155\/2020\/6590202","article-title":"The Influence of Immediately Loaded Basal Implant Treatment on Patient Satisfaction","volume":"2020","author":"Awadalkreem","year":"2020","journal-title":"Int. J. Dent."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1016\/j.jcms.2019.07.020","article-title":"Biomaterial-based bone regeneration and soft tissue management of the individualized 3D-titanium mesh: An alternative concept to autologous transplantation and flap mobilization","volume":"47","author":"Ghanaati","year":"2019","journal-title":"J. Cranio-Maxillofac. Surg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"78","DOI":"10.4103\/2231-0746.186143","article-title":"Success rate of implants placed in autogenous bone blocks versus allogenic bone blocks: A systematic literature review","volume":"6","author":"Motamedian","year":"2016","journal-title":"Ann. Maxillofac. Surg."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1111\/prd.12082","article-title":"Alveolar socket healing: What can we learn?","volume":"68","author":"Araujo","year":"2015","journal-title":"Periodontology 2000"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/j.ijom.2008.04.017","article-title":"Dimensional ridge alterations following socket preservation using a nanocrystalline hydroxyapatite paste: A histomorphometrical study in dogs","volume":"37","author":"Rothamel","year":"2008","journal-title":"Int. J. Oral Maxillofac. Surg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/0022-3913(71)90069-2","article-title":"Reduction of residual ridges: A major oral disease entity","volume":"26","author":"Atwood","year":"1971","journal-title":"J. Prosthet. Dent."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/S0901-5027(88)80047-X","article-title":"A classification of the edentulous jaws","volume":"17","author":"Cawood","year":"1988","journal-title":"Int. J. Oral Maxillofac. Surg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1563\/aaid-joi-D-14-00138","article-title":"Advanced platelet-rich fibrin: A new concept for cell-based tissue engineering by means of inflammatory cells","volume":"40","author":"Ghanaati","year":"2014","journal-title":"J. Oral Implant."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1007\/s11999-015-4192-2","article-title":"Platelet-rich concentrates differentially release growth factors and induce cell migration in vitro","volume":"473","author":"Schar","year":"2015","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2353","DOI":"10.1007\/s00784-016-1719-1","article-title":"Comparative release of growth factors from PRP, PRF, and advanced-PRF","volume":"20","author":"Kobayashi","year":"2016","journal-title":"Clin. Oral Investig."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1080\/09537104.2018.1445835","article-title":"A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: An experimental study in vivo","volume":"30","author":"Kubesch","year":"2019","journal-title":"Platelets"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1007\/s10856-017-5992-6","article-title":"Reduction of the relative centrifugal force influences cell number and growth factor release within injectable PRF-based matrices","volume":"28","author":"Wend","year":"2017","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"4551","DOI":"10.1016\/j.biomaterials.2007.06.037","article-title":"The potential impact of the preparation rich in growth factors (PRGF) in different medical fields","volume":"28","author":"Anitua","year":"2007","journal-title":"Biomaterials"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4956","DOI":"10.1016\/j.biomaterials.2009.05.055","article-title":"The use of platelet-rich plasma in bone reconstruction therapy","volume":"30","author":"Intini","year":"2009","journal-title":"Biomaterials"},{"key":"ref_16","first-page":"2","article-title":"PRGF plasma rich growth factors","volume":"3","author":"Anitua","year":"2004","journal-title":"Dialogue"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e37","DOI":"10.1016\/j.tripleo.2005.07.008","article-title":"Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution","volume":"101","author":"Dohan","year":"2006","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e56","DOI":"10.1016\/j.tripleo.2005.07.011","article-title":"Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part IV: Clinical effects on tissue healing","volume":"101","author":"Choukroun","year":"2006","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s00068-017-0767-9","article-title":"Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients\u2019 own inflammatory cells, platelets and growth factors: The first introduction to the low speed centrifugation concept","volume":"44","author":"Choukroun","year":"2018","journal-title":"Eur. J. Trauma Emerg. Surg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1186\/s40729-021-00393-0","article-title":"Efficacy of platelet-rich fibrin in promoting the healing of extraction sockets: A systematic review","volume":"7","author":"Becker","year":"2021","journal-title":"Int. J. Implant Dent."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e794","DOI":"10.1097\/SCS.0000000000004818","article-title":"Novel Application of Platelet-Rich Fibrin as a Wound Healing Enhancement in Extraction Sockets of Patients Who Smoke","volume":"29","author":"Asmael","year":"2018","journal-title":"J. Craniofac. Surg."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.jcms.2020.02.020","article-title":"The use of leukocyte- and platelet-rich fibrin in the management of soft tissue healing and pain in post-extraction sockets: A randomized clinical trial","volume":"48","author":"Javid","year":"2020","journal-title":"J. Craniomaxillofac. Surg."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"369273","DOI":"10.1155\/2015\/369273","article-title":"Influence of Leukocyte- and Platelet-Rich Fibrin (L-PRF) in the Healing of Simple Postextraction Sockets: A Split-Mouth Study","volume":"2015","author":"Marenzi","year":"2015","journal-title":"BioMed Res. Int."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.jobcr.2020.06.012","article-title":"Influence of platelet-rich fibrin on wound healing and bone regeneration after tooth extraction: A clinical and radiographic study","volume":"10","author":"Sharma","year":"2020","journal-title":"J. Oral Biol. Craniofac. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"28","DOI":"10.4103\/ams.ams_153_17","article-title":"Wound Healing and Bone Regeneration in Postextraction Sockets with and without Platelet-rich Fibrin","volume":"8","author":"Srinivas","year":"2018","journal-title":"Ann. Maxillofac. Surg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.jormas.2019.09.005","article-title":"Evaluation of different platelet-rich concentrates effects on early soft tissue healing and socket preservation after tooth extraction","volume":"121","author":"Ustaoglu","year":"2020","journal-title":"J. Stomatol. Oral Maxillofac. Surg."},{"key":"ref_27","first-page":"77","article-title":"Dental extractions for patients on oral antiplatelet: A within-person randomised controlled trial comparing haemostatic plugs, advanced-platelet-rich fibrin (A-PRF+) plugs, leukocyte- and platelet-rich fibrin (L-PRF) plugs and suturing alone","volume":"12","author":"Giudice","year":"2019","journal-title":"Int. J. Oral Implant."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1097\/SCS.0b013e31828b6dc3","article-title":"Influence of platelet-rich fibrin on alveolar ridge preservation","volume":"24","author":"Suttapreyasri","year":"2013","journal-title":"J. Craniofac. Surg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s10006-018-0680-3","article-title":"To assess the efficacy of socket plug technique using platelet rich fibrin with or without the use of bone substitute in alveolar ridge preservation: A prospective randomised controlled study","volume":"22","author":"Kumar","year":"2018","journal-title":"Oral Maxillofac. Surg."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Choukroun, J., and Ghanaati, S. (2017). Introducing the Low-Speed Centrifugation Concept. Platelet Rich Fibrin in Regenerative Dentistry: Biological Background and Clinical Indications. Platelet Rich Fibrin in Regenerative Dentistry: Biological Background and Clinical Indications, John Wiley & Sons Ltd.","DOI":"10.1002\/9781119406792.ch3"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1007\/s00068-017-0785-7","article-title":"Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: A proof of concept of LSCC (low speed centrifugation concept)","volume":"45","author":"Kubesch","year":"2019","journal-title":"Eur. J. Trauma Emerg. Surg."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1007\/s00784-017-2133-z","article-title":"Use of platelet-rich fibrin in regenerative dentistry: A systematic review","volume":"21","author":"Miron","year":"2017","journal-title":"Clin. Oral Investig."},{"key":"ref_34","unstructured":"Wallace, H.A., Basehore, B.M., and Zito, P.M. (2021). Wound Healing Phases, StatPearls Publishing."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1159\/000339613","article-title":"Wound repair and regeneration","volume":"49","author":"Reinke","year":"2012","journal-title":"Eur. Surg. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/bs.ai.2015.09.001","article-title":"Mechanism of Diapedesis: Importance of the Transcellular Route","volume":"129","author":"Filippi","year":"2016","journal-title":"Adv. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"407","DOI":"10.12968\/jowc.2013.22.8.407","article-title":"Wound healing and the role of fibroblasts","volume":"22","author":"Bainbridge","year":"2013","journal-title":"J. Wound Care"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S0094-1298(20)32467-6","article-title":"Physiology of the acute wound","volume":"25","author":"Lawrence","year":"1998","journal-title":"Clin. Plast. Surg."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1152\/physrev.00067.2017","article-title":"Wound Healing: A Cellular Perspective","volume":"99","author":"Rodrigues","year":"2019","journal-title":"Physiol. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"100035","DOI":"10.1016\/j.dentre.2022.100035","article-title":"Management of Dry Socket: New regenerative techniques emerge while old treatment prevails","volume":"2","author":"Kamal","year":"2022","journal-title":"Dent. Rev."}],"container-title":["Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-5354\/9\/11\/661\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:12:18Z","timestamp":1760145138000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-5354\/9\/11\/661"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,8]]},"references-count":40,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["bioengineering9110661"],"URL":"https:\/\/doi.org\/10.3390\/bioengineering9110661","relation":{},"ISSN":["2306-5354"],"issn-type":[{"value":"2306-5354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,8]]}}}